trans activation of cellular genes by hepatitis b virus proteins a possible mechanism of hepatocarcinogenesis

Báo cáo y học: "A novel nucleotide insertion in S gene of hepatitis B virus in a chronic carrier" pps

Ngày tải lên : 12/08/2014, 04:20

... insertion between aa121 and aa122 [20], 3-aa insertion between aa118 and aa119 [18], 2-aa deletion from aa110 to aa111 [17], 4-aa deletion from aa119 to aa122 [17], 3-aa deletion from aa113 to aa115 ... Chinese carriers Hepatology 1995, 21:273-278 Grethe S, Monazahian M, Bohme I, Thomssen R: Characterization of unusual escape variants of hepatitis B virus isolated from a hepatitis B surface antigen-negative ... Guestas ML, Mathet VL, Ruiz V, Minassian ML, Rivero C, Sala A, Corach D, Alessio A, Pozzati M, Frider B, Oubina JR: Unusual naturally occurring humoral and cellular mutated epitopes of hepatitis B...

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Báo cáo sinh học: " Cellular apoptosis induced by replication of hepatitis B virus: possible link between viral genotype and clinical outcome" pptx

Ngày tải lên : 18/06/2014, 18:20

... KS, Tan K, Leong AL, Tan GS: Molecular characterization of hepatitis B virus surface antigen mutants in Singapore patients with hepatocellular carcinoma and hepatitis B virus carriers negative ... cytometry analysis of apoptotic effect by HBV genome A, B, and C Flow cytometry analysis of apoptotic effect by HBV genome A, B, and C HepG2 cells were transiently transfected with HBV genome A, B, and ... Western blot analysis HBSP polyclonal anti-serum was acquired by boosting rabbit using an internal HBSP peptide: CDLNLGQDQQQPVRD (Biogenes, Germany) HBSP protein was detected by primary anti-HBSP antibody...

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Báo cáo hóa học: " Cellular apoptosis induced by replication of hepatitis B virus: possible link between viral genotype and clinical outcome" docx

Ngày tải lên : 20/06/2014, 01:20

... KS, Tan K, Leong AL, Tan GS: Molecular characterization of hepatitis B virus surface antigen mutants in Singapore patients with hepatocellular carcinoma and hepatitis B virus carriers negative ... cytometry analysis of apoptotic effect by HBV genome A, B, and C Flow cytometry analysis of apoptotic effect by HBV genome A, B, and C HepG2 cells were transiently transfected with HBV genome A, B, and ... Western blot analysis HBSP polyclonal anti-serum was acquired by boosting rabbit using an internal HBSP peptide: CDLNLGQDQQQPVRD (Biogenes, Germany) HBSP protein was detected by primary anti-HBSP antibody...

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Tài liệu Báo cáo khoa học: Limited suppression of the interferon-b production by hepatitis C virus serine protease in cultured human hepatocytes pptx

Ngày tải lên : 18/02/2014, 16:20

... Kojima H, Tawaraya H, Miyajima T, Kamimura T, Asakura H, Satoh A, Hirose S, Hijikata M, Kato N & Shimotohno K (1990) Prevalence of antibody against non -A, non -B hepatitis virus in Japanese patients ... thank Toshiko Maeta and Takashi Nakamura for their technical assistance Drs Kazuhito Naka (Kanazawa University) and Yasuo Ariumi (Okayama University) are also thanked for their valuable input in ... Medical and Biological Laboratories, Nagoya, Japan) or anti -b- actin serum (AC-15; Sigma, St Louis, MO, USA) was used in this study as a primary antibody Immunocomplexes were detected by a Renaissance...

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Báo cáo khoa học: Betulinic acid-mediated inhibitory effect on hepatitis B virus by suppression of manganese superoxide dismutase expression pot

Ngày tải lên : 16/03/2014, 01:20

... molecular mechanism of Tax transactivation Mol Cell Biol 17, 5156–5164 44 Uprichard SL, Boyd B, Althage A & Chisari FV (2005) Clearance of hepatitis B virus from the liver of transgenic mice by short ... BetA/CREB siCREB 100 * * CTL BetA BetA/CREB siCREB F 200 * * (Arbitrary units) 100 pCREB level by Western blot (Arbitrary units) 200 pCREB (Ser133) level by Western blot BetA/CREB 200 siCREB C BetA ... its antiamoebic, antibacterial and antitrichomonal activities [3] Recently, this herb was used for the treatment of a hepatitis B patient, according to an old recipe in a speciﬁc area of China...

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Báo cáo y học: "Hepatitis B virus core protein with hot-spot mutations inhibit MxA gene transcription but has no effect on inhibition of virus replication by interferon a" pptx

Ngày tải lên : 12/08/2014, 01:22

... detected by real time-PCR (a) ; the extracellular HBsAg level was assessed by abbot analysis (b) and the extra- and intracellular HBV DNA level (c and d respectively) was measured by real-time ... promoter activity was also examined by luciferase assay (b) and the level of Flag-tagged HBc protein was analyzed by Western blot using an anti-Flag antibody (d) In the stably-transfected Huh7 cells, ... MxA gene transcription by WT HBc or its hot-spot mutants has no impact on inhibition of HBV replication by IFN -a, indicating MxA may be independent of inhibition of HBV DNA replication by IFN-a...

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Báo cáo y học: " Prediction of conformational changes by single mutation in the hepatitis B virus surface antigen (HBsAg) identified in HBsAg-negative blood donors" ppsx

Ngày tải lên : 12/08/2014, 02:20

... clinical significance of surface antigen variants of hepatitis B virus J Viral Hepat 1997, 4(Suppl 1):11-20 26 Ireland JH, O’Donnell B, Basuni AA, Kean JD, Wallace LA, Lau GKK, Carman WF: Reactivity ... Selection of variants is usually indicated by certain serological markers, such as isolated anti-HBc, co-occurrence of both HBsAg and anti-HBs, and inconsistent HBsAg assay results [34] The presence of ... reported in association with medical and public health issues (problems in diagnostic assays and/or escape to vaccine/HBIg therapy) from published articles and GenBank database, focusing on variants...

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Báo cáo y học: "Epidemiology and Prevention of Hepatitis B Virus Infection"

Ngày tải lên : 02/11/2012, 11:12

... India B (Ba, Bj) C D E F G Southern China (Ba), Taiwan (Ba), Vietnam (Ba), Asians in the USA, Japan (Bj) China (Mainland and Taiwan), Japan, Thailand, Asians in the USA White Caucasians (Southern ... Table Geographic Distribution of HBV Genotypes Genotypes Distributions A (Aa, Ae) White Caucasians in Europe, Black Americans in US (Ae), Black Africans, South Africa (Aa), Asia (Aa), India B ... East Asia, China, sub-Saharan Africa and the Amazon Basin, where at least 8% of the population are HBV chronic carrier In these areas, 70–95% of the population shows past or present serological...

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Báo cáo y học: "Natural History and Clinical Consequences of Hepatitis B Virus Infection"

Ngày tải lên : 02/11/2012, 11:17

... the stage of reactivation, majority of patients remain HBeAg negative with positive HBeAb and their clinical presentation can be HBeAg negative chornic hepatitis B, but some patients may have seroreversion ... serology markers like HBeAg, HBeAb and HBsAb can be positive or negative except HBsAg and HBcAb (IgG form) remain positive HBeAg positive chronic hepatitis B Age at the time of infection is a strong ... program in newborns and the completion of a mass vaccination catch up program [10] In Taiwan, the universal hepatitis B vaccination in newborns has both dramatically decreased prevalence of HBsAg...

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Báo cáo y học: "Study of the early steps of the Hepatitis B Virus life cycle"

Ngày tải lên : 03/11/2012, 10:09

... by Hepatitis B Foundation of America; an Appropriation from the Commonwealth of Pennsylvania USA, Nucleonic Inc (PA USA) Drs Satishchandran C and Cathy Pachuk (Nucleonics Inc, PA, USA), Baohua ... the rat hepatocytes This binding could be inhibited by recombinant HBs but not by the recombinant LHBs The binding of SHBs with human hepatocytes was further supported by the observation from Bruin ... The Hepatitis B virus (HBV), which belongs to the hepadnavirus family, is a small circular DNA virus containing a nucleocapsid and an envelope HBV nucleocapsid contains a relatively small and...

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Báo cáo khoa học: Fidelity of hepatitis B virus polymerase pptx

Ngày tải lên : 31/03/2014, 01:20

... 2226 A: M13mp18: Primer 5385T: M13mp18: Primer 1212C: M13mp18: 5¢-GTT TAT CAG CTT GCT TT-CAA ATA GTC GAA CGA AAG5¢-TGA TAT TCA CAA ACG AA-ACT ATA AGT GTT TGC TTA5¢-TTT TAG ACA GGA ACG GT-AAA ATC ... dNTP), and were incubated at 37 °C The reaction was terminated by the addition of EDTA to a ﬁnal concentration of 50 mM in 95% formamide buffer Reaction products were denatured by incubating at 95 ... SDS/PAGE and immunoblot analysis The partially puriﬁed proteins were separated by 7.5% SDS/PAGE For immunoblot analysis, proteins were electrophoretically transferred to a PVDF blotting membrane...

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Báo cáo sinh học: " Common Genotypes of Hepatitis B virus prevalent in Injecting drug abusers (addicts) of North West Frontier Province of Pakistan" doc

Ngày tải lên : 18/06/2014, 18:20

... the association of unsafe injection practices and the transmission of blood borne viruses such as hepatitis B and C, Ebola, Lassa virus infections and malaria [34] A simple mass-action model was ... province Peshawar is located at the gateway of the transit trade route from Afghanistan easy availability of drugs at cheap prices are a permanent risk for Peshawar's youngster's and Pakistan's young ... awareness about the transmission of major communicable diseases like Hepatitis B virus, Hepatitis C virus and Human Immunodeficiency Virus The seropositivity rate of Hepatitis B surface antigen (HBsAg)...

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Báo cáo sinh học: " Serum levels of preS antigen (HBpreSAg) in chronic hepatitis B virus infected patients" potx

Ngày tải lên : 18/06/2014, 18:20

... carrier-state, HBeAg-positive, and HBeAg-negative chronic hepatitis B The inactive HBsAg carrier-state is characterized by the presence of HBsAg and anti-HBe, normal aminotransferase (ALT) level and ... top of each vertical bar The white bars represent the number of HBpreSAg negative patients and the black bars represent that of HBpreSAg positive patients Table 1: Average changes of HBpreSAg and ... HBpreSAg, hepatitis B pre-surface antigen; HBeAg, hepatitis B e antigen, anti-HBs, antibody to HBsAg; anti-preS, antibody to hepatitis B preS antigen; IFN-α, interferonalpha; ALT, alanine aminotransferase...

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Báo cáo sinh học: " Hepatitis B virus X protein interacts with β5 subunit of heterotrimeric guanine nucleotide binding protei" pptx

Ngày tải lên : 19/06/2014, 08:20

... was retained for ELISA analysis ELISA Analysis The HBV DNA level in the culture medium of the transfected HepG2 cells was analysed by ELISA (Murex HBsAg Version 3, ABBOTT) Page of (page number ... Journal 2005, 2:76 http://www.virologyj.com/content/2/1/76 Table 1: Production of HBV in Transfected HepG2 Cells by ELISA Analysis Wells Samples Absorbance at 450 nm (A4 50) A1 A2 A3 A4 A5 A6 A7 A8 ... yeast strain Y187 to test if the bait alone led to transcriptional activation α-galactosidase assay screening indicate negative interaction between bait and library Colonies remained white F3 and...

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Báo cáo hóa học: " Common Genotypes of Hepatitis B virus prevalent in Injecting drug abusers (addicts) of North West Frontier Province of Pakistan" potx

Ngày tải lên : 20/06/2014, 01:20

... the association of unsafe injection practices and the transmission of blood borne viruses such as hepatitis B and C, Ebola, Lassa virus infections and malaria [34] A simple mass-action model was ... province Peshawar is located at the gateway of the transit trade route from Afghanistan easy availability of drugs at cheap prices are a permanent risk for Peshawar's youngster's and Pakistan's young ... awareness about the transmission of major communicable diseases like Hepatitis B virus, Hepatitis C virus and Human Immunodeficiency Virus The seropositivity rate of Hepatitis B surface antigen (HBsAg)...

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Báo cáo hóa học: " HBVRegDB: Annotation, comparison, detection and visualization of regulatory elements in hepatitis B virus sequences" potx

Ngày tải lên : 20/06/2014, 01:20

... of alignments is available using Gbrowse For HBVRegDB the GBrowse software package was chosen because of its flexible configuration and efficient handling of large amounts of data, although a ... statistical track data, and textual information The five core tables of the HBVRegDB MySQL database contain additional taxonomic information, virus group relationships, web page search related data, and ... Hirahata M, Abe T, Tanaka N, Kuwana Y, Shigemoto Y, Miyazaki S, Suzuki Y, Sugawara H: Genome Information Broker for Viruses (GIB-V): database for comparative analysis of virus genomes Nucleic Acids...

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Báo cáo hóa học: " Elective caesarean section versus vaginal delivery for preventing mother to child transmission of hepatitis B virus – a systematic review" pot

Ngày tải lên : 20/06/2014, 01:20

... (morbidities related to the actual method of delivery) 14 NEONATAL MORBIDITY 15 MATERNAL MORTALITY 16 MATERNAL MORBIDITY (1)Maternal morbidity: types of maternal morbidity evaluated includes: febrile ... (graded C) According to the quality criteria listed above, we considered each included study was at high risk of bias and graded as category C Assessment of publication bias There was an insufficient ... http://www.virologyj.com/content/5/1/100 medical Literature Database (CBM) (1975 to 2008), China National Knowledge Infrastructure (CNKI) (1979 to 2008), VIP database (1989 to 2008) We also searched additional trials by scanning the...

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Báo cáo hóa học: " Application of real-time PCR to quantify hepatitis B virus DNA in chronic carriers in The Gambia" pptx

Ngày tải lên : 20/06/2014, 01:20

... to participate Thanks to Adam Jeng-Barry and Alasana Bah for laboratory assistance, Joseph Bass, Yusupha Bah, Lamin Giana and Mansour Nyang for field assistance We would like to thank Adrian V ... direct comparison to the International HBV DNA standard Data management The data obtained in the ABi real time machine after the PCR amplification and quantification of DNA was exported as an Excel ... hepatitis B: a randomised trial Lancet 2005, 365:123-129 Barbaro G, Zechini F, Pellicelli AM, Francavilla R, Scotto G, Bacca D, Bruno M, Babudieri S, Annese M, Matarazzo F, Di Stefano G, Barbarini...

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báo cáo hóa học:" Hepatitis B virus X protein interacts with β5 subunit of heterotrimeric guanine nucleotide binding protein" docx

Ngày tải lên : 20/06/2014, 04:20

... was retained for ELISA analysis ELISA Analysis The HBV DNA level in the culture medium of the transfected HepG2 cells was analysed by ELISA (Murex HBsAg Version 3, ABBOTT) Page of (page number ... Journal 2005, 2:76 http://www.virologyj.com/content/2/1/76 Table 1: Production of HBV in Transfected HepG2 Cells by ELISA Analysis Wells Samples Absorbance at 450 nm (A4 50) A1 A2 A3 A4 A5 A6 A7 A8 ... yeast strain Y187 to test if the bait alone led to transcriptional activation α-galactosidase assay screening indicate negative interaction between bait and library Colonies remained white F3 and...

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