hepatitis b virus and hepatitis d virus

... Interference between non-A, non -B and hepatitis < /b> B virus infection in chimpanzees J Med Virol 1983;11:191-205 Bradley DW, Maynard JE, McCaustland KA, Murphy BL, Cook EH, Ebert JW Non-A, non -B hepatitis < /b> ... acute hepatitis < /b> A virus and chronic hepatitis < /b> B virus infections J Med Virol 1983;11:207-213 Pontisso P, Gerotto M, Benvegnu L, Chemello L, Alberti A Coinfection by hepatitis < /b> B virus and hepatitis < /b> ... and by hepatitis < /b> C virus core protein J Hepatol 2002;37:855-862 45 Weltman MD, Brotodihardjo A, Crewe EB, Farrell GC, Bilous M, Grierson JM, Liddle C Coinfection with hepatitis < /b> B and C or B, C and...

Ngày tải lên: 02/11/2012, 09:51

... population based samples of adequate size on a consensus definition of eCHB and using standard HBV DNA assays is needed to estimate the true prevalence of e-CHB and its associated HBV variants and the ... (HBIG) is a sterile solution of ready-made antibodies against hepatitis < /b> B HBIG is prepared from human blood from selected donors who already have a high level of antibodies to hepatitis < /b> B and ... transmission of HBV, a large-scale, random-control study was conducted in China [67] In this study, nine hundred and eighty HBsAg carrier pregnant women were randomly divided into HBIG group and control...

Ngày tải lên: 02/11/2012, 11:12

... begins to drop With clearance of the infection, HBsAg and HBeAg disappear, and free HBsAb become detectable Presence of HBsAg, HBeAg, and high DNA level for greater than months implies progression ... infection establishes, the serology markers like HBeAg, HBeAb and HBsAb can be positive or negative except HBsAg and HBcAb (IgG form) remain positive HBeAg positive chronic hepatitis < /b> B Age at the ... Europe, India, and Africa Genotype B and C are common in Asia; genotype D, in southern Europe, the Middle East, and India; genotype E, in West Africa and South Africa; genotype F, in South and Central...

Ngày tải lên: 02/11/2012, 11:17

... cells and the HBx and HBV core mRNA levels and then the FXRa mRNA levels were determined via RT-PCR The RT-PCR bands were quantified and normalized relative to the b- actin mRNA control band using ... and distribution of lipid soluble vitamins and dietary fats, but also as signaling molecules that activate NRs and regulate bile acid and cholesterol metabolism [14,19] Additionally, it has been ... presence of different concentrations of CDCA for 24 h Total DNA was prepared from the cells and the HBV DNA levels was detected by PCR The DNA bands were quantified with ImageJ, version 1.3 5d (National...

Ngày tải lên: 22/03/2014, 21:21

... 52 HBV genomes was calculated and the graphic representation was created by WebLogo [47] The HP1 and HP2 regions are indicated, with stems being marked by cyan bars and the RNase-accessible joint ... carried out using a PTB1-specific shRNA and a PTB1 cDNA expressed from pSuper-neo and pcDNA3, respectively [34] PTB protein levels in treated and control cells were analyzed by western blot (bottom) ... named HP1 and HP2 (Fig 2B) HP1 contains two stems (S1 and S2) and a hexanucleotide loop (L1), whereas HP2 contains a 9-bp stem (S3) and a 25-nucleotide large loop (L2) HP1 and HP2 are joined by...

Ngày tải lên: 28/03/2014, 23:20

... infections Five cases had both genotypes A and D, cases had C and D, other cases had B and D and cases had genotypes B and C The distribution of mixed genotypes among AH and CAH patients is shown ... classified into four different subtypes that were afterward subdivided according to the antigenic determinants of HBsAg in adw (adw2 and adw4), ayw (ayw1, ayw2, ayw3, and ayw4), adr (adrq+ adrq-), and ... suffering from hepatitis < /b> and were diagnosed as HBV infection HBV was diagnosed based on clinical data, liver function tests, HBV serum markers and HBV DNA by PCR Serum samples were collected from patients...

Ngày tải lên: 18/06/2014, 18:20

... The data were entered in computer and double checked before analysis by SPSS for windows The means, percentages were calculated and compared between the sero-positive and sero-negative (HBVsAg) ... between our study and the others' should be taken cautiously Firstly different methods had been applied, in our study we aimed to detect antibodies using ELSIA, while some of these studies, DNA ... jaundice and blood transfusion, respectively 69(9%) and 21(3%) had traditional scares and tattooing, respectively One third of these women (231) had history of dental maneuvers HBsAg was detected...

Ngày tải lên: 18/06/2014, 18:20

... HBSP A BH3 HBSP B BH3 HBSP C BH3 21 31 32 35 LEEELPRLADADLHR LEEELPRLADEDLNH LEEELPRLADEGLNR Figure Alignment of BH3 domains of HBSPs Alignment of BH3 domains of HBSPs Alignment of the BH3 domains ... effect by HBV genome A, B, and C Flow cytometry analysis of apoptotic effect by HBV genome A, B, and C HepG2 cells were transiently transfected with HBV genome A, B, and C and incubated for 24 ... Germany) HBSP protein was detected by primary anti-HBSP antibody in 1:1000 dilution and secondary anti-rabbit antibody conjugated with horseradish peroxidase (Pierce, USA) in 1:5000 dilution Following...

Ngày tải lên: 18/06/2014, 18:20

... infections Five cases had both genotypes A and D, cases had C and D, other cases had B and D and cases had genotypes B and C The distribution of mixed genotypes among AH and CAH patients is shown ... classified into four different subtypes that were afterward subdivided according to the antigenic determinants of HBsAg in adw (adw2 and adw4), ayw (ayw1, ayw2, ayw3, and ayw4), adr (adrq+ adrq-), and ... suffering from hepatitis < /b> and were diagnosed as HBV infection HBV was diagnosed based on clinical data, liver function tests, HBV serum markers and HBV DNA by PCR Serum samples were collected from patients...

Ngày tải lên: 20/06/2014, 01:20

... The data were entered in computer and double checked before analysis by SPSS for windows The means, percentages were calculated and compared between the sero-positive and sero-negative (HBVsAg) ... between our study and the others' should be taken cautiously Firstly different methods had been applied, in our study we aimed to detect antibodies using ELSIA, while some of these studies, DNA ... jaundice and blood transfusion, respectively 69(9%) and 21(3%) had traditional scares and tattooing, respectively One third of these women (231) had history of dental maneuvers HBsAg was detected...

Ngày tải lên: 20/06/2014, 01:20

... HBSP A BH3 HBSP B BH3 HBSP C BH3 21 31 32 35 LEEELPRLADADLHR LEEELPRLADEDLNH LEEELPRLADEGLNR Figure Alignment of BH3 domains of HBSPs Alignment of BH3 domains of HBSPs Alignment of the BH3 domains ... effect by HBV genome A, B, and C Flow cytometry analysis of apoptotic effect by HBV genome A, B, and C HepG2 cells were transiently transfected with HBV genome A, B, and C and incubated for 24 ... Germany) HBSP protein was detected by primary anti-HBSP antibody in 1:1000 dilution and secondary anti-rabbit antibody conjugated with horseradish peroxidase (Pierce, USA) in 1:5000 dilution Following...

Ngày tải lên: 20/06/2014, 01:20

... are separately described using the standard numbering [32] Annotated DNA elements Two Direct Repeat DNA primer-binding sites – DR1 and DR2 – are involved in replication and may be preferential ... research into HBV [14-17] We describe here a genome-based public domain database for the Hepadnaviridae The database contains data on individual sequences and groups of sequences and facilitates ... analysis, design of basis for HBVRegDB (e.g content and structure) and drafted the manuscript SKR developed web interface and a comparative genomic analysis tool with an integrated underlying HBV viral...

Ngày tải lên: 20/06/2014, 01:20

... antibody to HBVsAg and incubated for 60 minutes at 37°C After washing, 100µL of peroxidase-conjugated mouse monoclonal antibodies against HBsAg was added to each well and the plate was incubated ... exposed to aflatoxin in their diets and examined the association of vitamins A and E concentrations, HIV status, AF-ALB levels and hepatitis < /b> B virus (HBV) infection Methods Study location, design and ... association between aflatoxin B1 albumin (AF-ALB) adduct levels and vitamins A and E in Ghanaians Obuseh et al [26] found a significant inverse relationship between AF-ALB and vitamin A and a non-significant...

Ngày tải lên: 20/06/2014, 08:20

... samples SS and NK designed the HIV antigen and generated the monoclonal antibodies TS, NK and KP conceived and designed the experiments SS, TS, NK and KP interpreted the data and prepared the manuscript ... mAb 2 1B (BiomAb 2 1B) , immobilized on SA-coated microtiter wells, to capture anti-HIV-1 antibodies and HBsAg, respectively Captured anti-HIV-1 antibodies were detected with Tb3+ chelate-labeled ... the absence and presence of anti-HIV-1 antibody binders The corresponding LLOD for anti-HIV-1 antibody detection cannot be designated for the reason mentioned above Nevertheless, it is evident...

Ngày tải lên: 11/08/2014, 00:22

... relationship between codon usage pattern of HBV and its host Result Synonymous coodn usage in HBV The C% and U% were higher than A% and G%, and C3% and U3% were higher than A3% and G3% in HBV (Table 1) ... of HBV were downloaded from the National Center for Biotechnology Information (NCBI) (http://www.ncbi.nlm.nih.gov/Genbank/) and detailed information about the viruses were listed in Table Table ... alignment and drafted the manuscript., MLW and FXZ participated in the sequence alignment SDZ, GL and YW participated in the design of the study and performed the statistical analysis XQH conceived of...

Ngày tải lên: 11/08/2014, 21:22

... cccDNA standard plasmid from 10 to 10 10 copies/μL were detected by real-time PCR assay and used to prepare the standard curve for quantitation of HBV cccDNA The standard curve of HBV cccDNA ... and electrophoretically transferred onto polyvinylidene difluoride (PVDF) membrane (Hybond-P, Amersham Pharmacia Biotech) Blots were blocked with 5% of skim milk in TBS-T buffer for h and subsequently ... (nucleotides 1151-1684, of Accession number AM282986) were analyzed using results of CDS-Plotcon and Alidot programmes provided by the database HBVRegDB [23] In brief, a set of 32 completed HBV genomes...

Ngày tải lên: 12/08/2014, 01:21

... and 4) HBS1R (positions 668 to 691) Serum samples were treated as described above and subjected to two rounds of amplification sequentially with outer (FHBS1 and RHBS1) and inner (FHBS2 and RHBS2) ... genotype, subtype, viral load, and HBeAg antibody status and examined those and other clinical characteristics (including age and gender) of the patients included in our study to determine if ... determinants of HBsAg in adw (adw2 and adw4), ayw (ayw1, ayw2, ayw3, and ayw4), adr (adrq adrq), and ayr Subtype a is common to the majority of viruses and is related to a neutralizing epitope Divergence...

Ngày tải lên: 12/08/2014, 04:20

... nothing in Dar Fur region particularly Thus the current study aimed to determine the seroprevalence and the possible risk factors for HBV and HCV among blood donors in Nyala Materials and methods This ... = 400) attending Nyala Teaching Hospital and randomly selected as study subjects and enrolled after interviewing questionnaire include the demographic information (age, sex and residence), history ... willingly donate their blood Results showed that the seroprevalence of HBV and HCV among blood donors in Nyala was (6.25%) and (0.65%) respectively, this indicates an intermediate HBV seroprevalence...

Ngày tải lên: 12/08/2014, 04:20

... reactive blood donors, representing about 9% of all the recruited donors Next, HBV DNA load in the positive donors was measured As shown in Figures 1B and 1C, donors 3, 4, and had several hundred virus ... research papers will be: available free of charge to the entire biomedical community peer reviewed and published immediately upon acceptance cited in PubMed and archived on PubMed Central yours — ... showed that donors and were more contagiousness, as they were HBsAg, HBeAg and anti-HBc positive (Table 2) Although HBsAg in donor was nega- tive (Table 2), HBV DNA testing proved there were few virus...

Ngày tải lên: 12/08/2014, 04:20

... three viruses of HIV, HBV, and HCV on 2167 blood samples taken from blood donors, the rates for HBV, HCV, and HIV were 4.6%, 2.9%, and 6%, respectively [22] Many studies have been conducted in ... findings and revised the manuscript EGH obtained and organized the clinical samples from HIV positive, FE performed analyses of data, HB carried out ELISA test, and YP performed wrote and editing ... entire biomedical community peer reviewed and published immediately upon acceptance cited in PubMed and archived on PubMed Central yours — you keep the copyright BioMedcentral Submit your manuscript...

Ngày tải lên: 12/08/2014, 04:20