deep sequencing of the hepatitis b virus genome analysis of multiple samples by implementation of the illumina platform
Methods in molecular biology vol 1540 hepatitis b virus methods and protocols
... intracellular calcium metabolism by the Bouchard lab; detection, cloning, and sequencing < /b> of HBV markers in laboratory-generated and clinical samples by Dandri, Huang, Jilbert, Weiland, and Tong ... Goddard, Lale Bildrici-Ertekin, Xiaohe Wang, and Andrea Cuconati 17 Deep < /b> Sequencing < /b> of the Hepatitis B Virus Genome: Analysis of Multiple Samples by Implementation of the Illumina Platform ... discovery by Tavis and Arbuthnot groups; novel and already established animal and in vivo-derived models detailed by the groups of Chen, Lu, Menne, Ou, and Su; and methods contributed by the Robek lab...
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Báo cáo khoa học: E2A participates in a fine control of pre-mature B-cell apoptosis mediated by B-cell receptor signaling via transcriptional regulation of survivin, IAP2 and caspase-8 genes pot
... activities [24] Based on these results, in this study, as a first step in elucidating the participation of B cell-specific factors in pre-mature B- cell apoptosis mediated by BCR stimulation by analyzing ... mediated by PMA/ionomycin treatment, but also that their own transcription is not influenced by PMA/ionomycin Therefore, the apoptotic cell death of DT40 mediated by PMA/ionomycin treatment must be ... up to 24 h, and subjected to SDS–PAGE followed by immunoblotting Antibody binding was detected using secondary antibodies conjugated to horseradish peroxidase, and then data analysis was performed...
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Báo cáo khoa học: Arg143 and Lys192 of the human mast cell chymase mediate the preference for acidic amino acids in position P2¢ of substrates pdf
... (VVLFSGVL), the efficiency of cleavage by HC dropped by a factor of 4–5 (Fig 6B) In contrast, the HC double mutant preferred this substrate over the HC consensus site by a factor of 2–5 (Fig 6C and data ... determined by phage display analysis, and the rat granzyme B (LIETDSGL) [14] and EK consensus sequences (LDDDDKGL) Neither the granzyme B, the EK nor the thrombin substrate was cleaved at all by HC ... followed by a His6-tag in the C-terminus of capsid protein 10 Thereby, the phages display a random nonamer on their surface, and by interactions of the His6-tag the phages can be immobilized on...
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Báo cáo y học: "Specific eradication of HIV-1 from infected cultured cells" doc
... virus production was estimated using MAGI assay on TZM-bl cells at 48 h PI (c) Same as (a) but cells viability was estimated by the MTT assay as described in [28] (dI) and (dII) Same as (a) but ... viral integration by the INS and INrs peptides, combined with the prevention of virion production by the protease inhibitor, not only resulted in blocking of HIV-1 infection but also in extermination ... can be seen (Fig 1c) about 40% of the cultured cells died by the eighth day PI following the addition of the Mix This percentage may represent the relative amount of virus infected cells, probably...
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Báo cáo sinh học: "The combined transduction of copper, zincsuperoxide dismutase and catalase mediated by cell-penetrating peptide, PEP-1, to protect myocardium from ischemia-reperfusion injury" ppt
... PEP-1-CAT on Bcl-2 and Bax expression Bcl-2 and Bax expression was detected by Western blot as described in Materials and Methods (A), and quantified by normalization to tubulin (B and C) Bcl-2/Bax ratio ... lyses buffer Western blot analysis was performed using procedures established in our laboratory The following antibodies were used: rabbit anti-Bax (Santa Cruz Biotechnology), mouse anti-Bcl-2 ... Bcl-2/Bax ratio while reduced Bax levels Combination of PEP-1-SOD1 and PEP-1-CAT further increased Bcl-2 level and Bcl-2/ Bax ratio, suggesting that PEP-1-SOD1 and PEP-1-CAT combination may better...
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Tài liệu Báo cáo khoa học: Properties of ecdysteroid receptors from diverse insect species in a heterologous cell culture system – a basis for screening novel insecticidal candidates docx
... The LdEcRB fragment was removed from pBluescriptKS + LdEcRB [31], and the vector pcDNA3.1- (Invitrogen, Carlsbad, CA, USA) was linearized by restriction digestion with XbaI and BamHI Both restriction ... VP16-USP ⁄ DDBD on MurA-inducible transcriptional activity at 2.5 lM (A) DmEcRB1 and DmEcRB2 with VP16-DmUSP and VP16-DmUSP ⁄ DDBD (B) LdEcRA and LdEcRB with VP16-LdUSP and VP16-LdUSP ⁄ DDBD (C) Cross-species ... EcRE as a labeled probe Asterisk designates shift band All extracts were equilibrated by b- galactosidase activity prior to loading Densitometry readings corresponding to designated shift bands are...
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Báo cáo khoa học: Characterization of a second proliferating cell nuclear antigen (PCNA2) from Drosophila melanogaster docx
... was followed by dialysis in NaCl ⁄ Pi at °C for h The proteins bound to GST Sepharose- 4B beads were analyzed by western blotting with anti-T7 or anti-GST serum (B) In vivo interaction between DmPCNA2 ... Pharmacia Biotech, Piscataway, NJ) Animals were fed water and standard rabbit food and maintained on a 12 h light/dark cycle Polyclonal antiserum to the peptide was raised in rabbits by subcutaneous ... three times with the same buffer, solubilized in SDS ⁄ PAGE sample buffer, and analyzed by western blotting For generation of antibodies to DNA polymerase d, the purified recombinant DNA Fractionation...
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báo cáo hóa học:" Regulatory activity of azabisphosphonate-capped dendrimers on human CD4+ T cell proliferation enhances ex-vivo expansion of NK cells from PBMCs for immunotherapy" potx
... μg/ml in PBS and 18 μl of a RNAse solution for 30 RT and washed with PBS containing 5% fetal calf serum before flow cytometry analysis Equilibrium binding assay Cells in triplicates were incubated ... anti-CD3/CD28 coated beads, +/- 3a-G1 (Fig 3c) In http://www.translational-medicine.com/content/7/1/82 order to reject the possibility that our CFSE analysis could be biased by the exclusion of ... but most of the cells maintained their DNA integrity Conversely, the proportion of mitotic events were reduced by 72% (15.8% to 4.2%) (Fig 3c, bottom) Given the fact that 3aG1 by itself is able...
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báo cáo hóa học: " Quality of life among adolescents with sickle cell disease: mediation of pain by internalizing symptoms and parenting stress" pdf
... English was not their first language Eligible patients were contacted by clinic staff during clinic visits or by telephone about participation Of 71 eligible participants who were contacted regarding ... included in the model) Subsequently, where appropriate, regression models were computed to test mediation based on a procedure described by Baron and Kenny[32] as were followup Sobel's tests of the ... report of BASC anxiety, BASC depression http://www.hqlo.com/content/6/1/60 Table 2: Description of variables under study Variables Mean SD Range Parent PPQ Pain Frequency BASC Anxiety BASC Depression...
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Báo cáo khoa học " Structural characterization of immunostimulating polysaccharide from cultured mycelia of Cordyceps militaris " doc
... refractionated by gel filtration with Sepharose CL- 6B (B) Molecular weights of standard dextrans and CPMN Fr III determined by Sepharose CL- 6B gel filtration chromatography dards The data summarized in Table ... to be fully methylated, as indicated by the disappearance of the band at 3400 cmÀ1 Fig Immunostimulating effects of polysaccharide, CPMN Fr III, purified by DEAE cellulose and Sepharose CL- 6B chromatography ... then was quenched by addition of sodium bicarbonate The organic layer was separated with a syringe and products were isolated and acetylated Glycosidic linkage was analyzed by GC–MS on a Micromass...
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Báo cáo y học: " Monocytes are essential for inhibition of synovial T-cell glucocorticoid-mediated apoptosis in rheumatoid arthritis" docx
... allophycocyanine-conjugated antiCD3 antibody (555335; BD Biosciences) and incubated with Annexin V and 7-amino-actinomycin D (7-AAD) as specified by the manufacturer (559763; BD Biosciences) and analyzed by flow cytometry ... protein-conjugated anti-CD14 antibody (340585; BD Biosciences) and with mouse IgG1 phycoerythrin-conjugated anti-CD3 antibody (HIT3a; BD Biosciences), followed by incubation with Annexin V (TA5532; ... antibody (555335; BD Biosciences), followed by incu- bation with Annexin V and 7-AAD as specified by the manufacturer (559763; BD Biosciences) and analyzed by flow cytometry T cells were gated as...
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Báo cáo y học: "CD14 mediates the innate immune responses to arthritopathogenic peptidoglycan–polysaccharide complexes of Gram-positive bacterial cell walls" docx
... NF- B is an essential component of TLR signaling [8] The activity of NF- B is controlled by interaction an inhibitory molecule known as I B Cell stimulation induces degradation of I B, thereby ... Wang J, Sartor RB, Zhang C, Bender D, Dalldorf FG, Schwab JH: Reactivation of arthritis induced by small bowel bacterial overgrowth in rats: role of cytokines, bacteria, and bacterial polymers ... strongly inhibited but not abolished (Fig 6) It is possible that resident nonphagocytic cells within joints can be activated by PG-PS via CD14-independent mechanisms In this regard, Kyburz and coworkers...
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báo cáo khoa học: "A Review of Duodenal Metastases from Squamous Cell Carcinoma of the Cervix Presenting as an Upper Gastrointestinal Bleed" potx
... identified by this strategy were searched with the selection of additional relevant publications for review and analysis Table provides a comprehensive review of the published cases of small bowel ... As the bacterial counts in the small bowel are absent or considerably lower than the large bowel, there is minimal exposure to the potentially carcinogenic chemical products of bacterial breakdown ... small bowel (Table 1) The most common site of metastases was the ileum (3 cases) followed by the duodenum (2 cases) and the jejunum (1 case) Indirect small bowel involvement was also noted by Ewing...
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Báo cáo khoa học: "In vitro characterization of cells derived from chordoma cell line U-CH1 following treatment with X-rays, heavy ions and chemotherapeutic drugs" pdf
... treatment of chordoma by carbon ions at our institute may be attributed to such characteristics even SOBP carbon ions are not as high RBE or low OER as monoenergetic high LET carbon beam [23,40] Conclusion ... (approximate 3.6 kb and 1.5 kb) were subjected to sequencing < /b> reaction using the p53 exon-specific primers supplied by Nippon Gene (Toyama, Japan) and Big-Dye Terminator Cycle Sequencing < /b> FS Ready ... PI-stained 10,000 cells were analyzed by BD FACSCalibur (Beckton Dickinson, Japan) to obtain the DNA content histogram Cell cycle characteristics were analyzed by Modfit program on Mac OS The DNA...
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Báo cáo y học: "Monocytes are essential for inhibition of synovial T-cell glucocorticoid-mediated apoptosis in rheumatoid arthritis" pdf
... allophycocyanine-conjugated antiCD3 antibody (555335; BD Biosciences) and incubated with Annexin V and 7-amino-actinomycin D (7-AAD) as specified by the manufacturer (559763; BD Biosciences) and analyzed by flow cytometry ... protein-conjugated anti-CD14 antibody (340585; BD Biosciences) and with mouse IgG1 phycoerythrin-conjugated anti-CD3 antibody (HIT3a; BD Biosciences), followed by incubation with Annexin V (TA5532; ... antibody (555335; BD Biosciences), followed by incu- bation with Annexin V and 7-AAD as specified by the manufacturer (559763; BD Biosciences) and analyzed by flow cytometry T cells were gated as...
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báo cáo khoa học: "Enhancive effects of Lewis y antigen on CD44-mediated adhesion and spreading of human ovarian cancer cell line RMG-I" pps
... of Biotechnology; SABC kit from Beijing Zhongshan Golden Bridge Biotechnology Co., Ltd; HA from Hefei Bomei Biotechnology Co., Ltd; DMEM culture medium from Gibco Co.; fetal bovine serum (FBS) ... anti-rabbit TRITC-labeled secondary antibody and goat anti-mouse FITC-labeled secondary antibody were diluted to 1:200 Cells were blocked by normal goat serum for 30 min, added with primary antibody ... were treated by the one-step immunofluorescence duallabeling method In brief, mouse anti-human Lewis y antibody and rabbit anti-human CD44 antibody were diluted to 1:100 as primary antibody solutions;...
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báo cáo khoa học: " Effect of filtration on morphine and particle content of injections prepared from slow-release oral morphine tablets" ppt
... mixtures was that of water to enable the volumes to be calculated Aliquots of each rinse were taken for morphine analysis Cotton ball filter A cotton ball (Home Brand Cotton Balls, Australia) was cut ... with 20× objective) and the other a linear scale (0-10 by 0.1 unit) whose length was 400 μm with 20× objective The rectangle and scale were calibrated using the ruled lines of a Neubauer blood cell ... particle-free, and this will be described under the combination filtration procedure Hot extraction, unfiltered The tablet could only be coarsely crushed in the crucible, but everything melted or...
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Báo cáo y học: " Analysis of Prototype Foamy Virus particle-host cell interaction with autofluorescent retroviral particles" ppt
... stomatitis virus J Virol 2004, 78:10920-10926 35 Lampe M, Briggs JA, Endress T, Glass B, Riegelsberger S, Kräusslich HG, Lamb DC, Bräuchle C, Müller B: Double-labelled HIV-1 particles for study of virus- cell ... determined by flow cytometry 72 - 96 h after infection as described above Biochemical analysis of PFV particles Biochemical analysis of purified PFV particles was essentially performed as described ... T, Lampe M, Briggs JA, Krausslich HG, Brauchle C, Muller B, Lamb DC: HIV-1-cellular interactions analyzed by single virus tracing Eur Biophys J 2008, 37:1291-1301 22 Jouvenet N, Bieniasz PD,...
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Báo cáo y học: "Adoptive transfer of splenocytes to study cell-mediated immune responses in hepatitis C infection using HCV transgenic mice" pptx
... were depleted of red blood cells (RBC) using RBCs lysis buffer (155 mM NH4Cl, 10 mM KHCO3, and 0.1 mM EDTA) The cellular suspension was washed three times by adding 0.1% BSA in PBS and centrifuged ... or CD4 + (Caltag Laboratories, Hornby, ON) The cells were washed as above, fixed and permeabilized using Caltag reagent A and B fixation-permeabilization solutions (Caltag Laboratories) The cells ... or recombinant HCV poly vaccinia Page of 13 labeled lymphocytes were incubated for 15 at 37°C The staining was quenched by adding volumes ice-cold complete RPMI media followed by a incubation...
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- xây dựng quy trình phát hiện đột biến rta181vt và rtn236t kháng adefovir của virus viêm gan b hepatitis b virus bằng kỹ thuật realtime pcr
- hepatitis b virus and hepatitis d virus
- —hiv hepatitis b virus coinfection
- hepatitis b virus genetic diversity disease pathogenesis
- trans activation of cellular genes by hepatitis b virus proteins a possible mechanism of hepatocarcinogenesis
- the hepatitis b virus
- validation of a sensitive and speci fi c real time pcr for detection and quantitation of hepatitis b virus covalently closed circular dna in plasma of chronic hepatitis b patients
- natural history of hepatitis b virus in hiv infected patients
- promoter precore and core regions of hepatitis b virus and their clinical signifi cance
- chiang lean teik ng li teh liu den en shieh chun ching lin 2003 cytotoxicity and anti hepatitis b virus activities of saikosaponins from bupleurum species planta med 69 8 pp 705 709
- generation of replication competent hepatitis b virus genome from blood samples for functional characterization
- detection of hepatitis b virus particles released from cultured cells by particle gel assay
- detection of hepatocyte clones containing integrated hepatitis b virus dna using inverse nested pcr
- intracytoplasmic transport of hepatitis b virus capsids
- hepatitis b virus infection of heparg cells heparg hntcp cells and primary human hepatocytes
Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các mục tiêu của chương trình xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản khảo sát chương trình đào tạo gắn với các giáo trình cụ thể tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn hệ số công suất cosp fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến tốc độ rôto n fi p2 thông tin liên lạc và các dịch vụ phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng 9 tr 25