the hepatitis b virus

... binding could be inhibited by recombinant HBs but not by the < /b> recombinant LHBs The < /b> binding of SHBs with human hepatocytes was further supported by the < /b> observation from Bruin et al [39] They have found ... of the < /b> two types [3, 4] The < /b> life cycle of HBV is believed to begin when the < /b> virus attaches to the < /b> host cell membrane via its envelope proteins Then, the < /b> viral membrane fuses with the < /b> cell membrane ... growing body of data, recently most of the < /b> published information about the < /b> early stages of hepadnavirus infection is derived from duck hepatitis < /b> B virus (DHBV), since robust DHBV infectable tissue...

Ngày tải lên: 03/11/2012, 10:09

... calibrator = - ΔΔCt In this study, the < /b> reference gene was beta-globin, the < /b> target gene was the < /b> cccDNA of HBV co-transfected with siRNA, and the < /b> calibrator was cells transfected with only the < /b> HBV ... Inhibition of hepatitis < /b> B virus in mice by RNA interference Nat Biotechnol 2003, 21:639-644 Peng J, Zhao Y, Mai J, Pang WK, Wei X, Zhang P, Xu Y: Inhibition of hepatitis < /b> B virus replication by ... characterization of the < /b> interaction between human La and hepatitis < /b> B virus RNA J Biol Chem 2004, 279:43437-43447 Donello JE, Beeche AA, Smith GJ, Lucero GR, Hope TJ: The < /b> hepatitis < /b> B virus posttranscriptional...

Ngày tải lên: 12/08/2014, 01:21

... mothers with HBV infection, and liver transplant recipients treated with hepatitis < /b> B immunoglobulin (HBIg) therapy [3-5] Recognition of the < /b> ’a’ determinant by antibody against HBsAg (anti-HBs) ... Virology In Hepatitis < /b> B Virus Edited by: Lai CL, Locarnini S London, International Medical Press Ltd; 2002:9-22 Weber B: Diagnostic Impact of the < /b> Genetic Variability of the < /b> Hepatitis < /b> B Virus Surface ... recognition of one of the < /b> ’a’ determinant epitopes by monoclonal anti-HBs [7] Hence, the < /b> substitution site is important because it may disturb the < /b> disulphide bonds, leading to the < /b> alteration of loop...

Ngày tải lên: 12/08/2014, 02:20

... Serology Subjects were tested for HBV core antibody (anti-HBc) and if indicated HBV surface antigen (HBsAg) and HBV e antigen (HBeAg) Anti-HBc was measured by radioimmunoassay (RIA) (Sorin Biochemica, ... antigen (HBsAg) in serum HBV e antigen (HBeAg) is generally used as secondary marker to indicate high levels of virus in the < /b> blood The < /b> minority of chronic HBV carriers in whom HBeAg can be detected ... load, even before they lose HBeAg from the < /b> blood As shown in this study there is a significant decline in viral load with age in both HBeAg positive and HBeAg negative carriers Discussion The < /b> results...

Ngày tải lên: 20/06/2014, 01:20

... Figure The < /b> genetic characteristic of HBV isolated different countries Based on the < /b> subtypes of HBV, the < /b> plots for the < /b> subtype adw were generally divided into two groups, while the < /b> other three subtypes ... HBV were downloaded from the < /b> National Center for Biotechnology Information (NCBI) (http://www.ncbi.nlm.nih.gov/Genbank/) and detailed information about the < /b> viruses were listed in Table Table The < /b> ... a cloned hepatitis < /b> B virus genome, subtype ayr: comparison with genomes of the < /b> other three subtypes J Gen Virol 1986, 67 ( Pt 11):2305-2314 10 Bartholomeusz A, Schaefer S: Hepatitis < /b> B virus genotypes:...

Ngày tải lên: 11/08/2014, 21:22

... to the < /b> risk factors responsible for the < /b> spread of this dual HDV/HBV infection Abbreviations HDV: hepatitis < /b> delta virus; HBV: hepatitis < /b> B virus; HCC: hepatocellular carcinoma; HBSAG: hepatitis < /b> B ... Ayub Med Coll Abbottabad 2009, 21:1-3 Seetlani NK, Abbas Z, Raza S, Yakoob J, Jafri W: Prevalence of Hepatitis < /b> D in HBsAg positive patients visiting liver clinics JPMA 2009, 59:434 Zuberi BF, ... the < /b> dual infection of HDV and HBV is available from Punjab, Pakistan that is the < /b> largest province of this country Therefore, the < /b> aim of the < /b> current study was to estimate the < /b> co-infection of hepatitis...

Ngày tải lên: 12/08/2014, 01:22

... contagiousness, as they were HBsAg, HBeAg and anti-HBc positive (Table 2) Although HBsAg in donor was nega- tive (Table 2), HBV DNA testing proved there were few virus copies (Fig 1B) The < /b> major HBV strains ... proves the < /b> high mutation rate of HBV A DL2000 10 11 281bp 132bp 109bp Counts of samples B B C D Genotype of HBV Figure of HBV reactive blood donors Genotyping Genotyping of HBV reactive blood ... available sequences from the < /b> GenBank database were used to construct the < /b> tree with the < /b> Clustal W v1.83 program Figures in the < /b> lower part of the < /b> tree are the < /b> blood donors' numbers; characters in the...

Ngày tải lên: 12/08/2014, 04:20

... [27] The < /b> maturation stage of the < /b> HBV has To explain the < /b> effect of mutations of core protein in the < /b> biological process of the < /b> cell, we predicted the < /b> major functions of the < /b> core protein of hepatitis < /b> ... core protein were associated with more severe liver fibrosis Abbreviations used in this paper (HBV): Hepatitis < /b> B virus; (CHB): Chronic hepatitis < /b> B; (CTL): Cytotoxic T lymphocyte Competing interests ... evaluated Twenty nine male subjects with detectable HBV DNA and candidate for liver biopsy were enrolled for the < /b> analysis of the < /b> prevalence of HBc mutations The < /b> assessed laboratory parameters were...

Ngày tải lên: 12/08/2014, 04:21

... anti-HBe xuất muộn Sự có mặt kháng thể anti-HBe tác dụng chống tái nhiễm HBV, mà biểu mắc HBV B ng Các dấu ấn thò nhiễm HBV Dấu ấn HBsAg HBeAg anti-HBs anti-HBc IgM anti-HBc IgG anti-HBe HBV DNA ... HBeAg(+) HBsAg(+) tỷ lệ lây nhiễm cao, đặc biệt phụ nữ có thai, HBsAg(+) cần theo dõi HBeAg, HBeAg(+) hầu hết họ b lây nhiễm - Kháng nguyên C (HbcAg) : HbcAg kháng nguyên lõi (core) lớp vỏ HbsAg ... thực b ớc biến nạp nhằm đưa sản phẩm nối vào chủng biểu E coli BL21(DE3) 3.7.8 Biến nạp vector tái tổ hợp vào tế b o chủ E coli a Chuẩn b tế b o khả nạp - Cấy 50µl E coli BL21(DE3) (dùng để biến...

Ngày tải lên: 09/02/2015, 04:05

... acute hepatitis < /b> A virus and chronic hepatitis < /b> B virus infections J Med Virol 1983;11:207-213 Pontisso P, Gerotto M, Benvegnu L, Chemello L, Alberti A Coinfection by hepatitis < /b> B virus and hepatitis < /b> ... Xing Bing Xue Za Zhi 1999 ;20:141-143 Zarski JP, Bohn B, Bastie A, Pawlotsky JM, Baud M, Bost-Bezeaux F, Tran van Nhieu J, et al Characteristics of patients with dual infection by hepatitis < /b> B and ... Interference between non-A, non -B and hepatitis < /b> B virus infection in chimpanzees J Med Virol 1983;11:191-205 Bradley DW, Maynard JE, McCaustland KA, Murphy BL, Cook EH, Ebert JW Non-A, non -B hepatitis...

Ngày tải lên: 02/11/2012, 09:51

... 2005 of HBV infection was based on positive HBsAg The < /b> case definition for confirmed acute HBV infection includes the < /b> presence of HBsAg combined with IgM antibody to the < /b> HBV core (anti-HBc) A child ... Canadianborn if the < /b> child’s birthplace was reported to be in Canada, or if the < /b> date of the < /b> mother’s immigration to Canada preceded the < /b> child’s date of birth A person who did not meet these criteria ... newly diagnosed HB cases in children were reported to the < /b> EHSSS Of these, clinically recognized HB cases with neither HBsAg nor IgM anti-HBc results were excluded Thus, 195 HB cases in children...

Ngày tải lên: 02/11/2012, 11:08

... Southeast Asia Before HBV vaccine was integrated into the < /b> routine immunization program, the < /b> proportion of babies that become HBV carriers is about 10-30% for mothers who are HBsAg-positive but ... Quantitative analysis of HBV DNA level and HBeAg titer in hepatitis < /b> B surface antigen positive mothers and their babies: HBeAg passage through the < /b> placenta and the < /b> rate of decay in babies J Med Virol ... Occult Hepatitis < /b> B Occult hepatitis < /b> B is defined by the < /b> presence of HBV DNA in serum or liver in the < /b> absence of HBsAg [42,43] Serum HBV level is usually less than 104 copies/ml Although occult HBV...

Ngày tải lên: 02/11/2012, 11:12

... infection establishes, the < /b> serology markers like HBeAg, HBeAb and HBsAb can be positive or negative except HBsAg and HBcAb (IgG form) remain positive HBeAg positive chronic hepatitis < /b> B Age at the < /b> time ... knowledge about the < /b> HBV behavior in these individuals There is much more to be understood about mutations and their impacts on the < /b> clinical course and long-term outcome of hepatitis < /b> B infection ... as HBsAg carrier * During the < /b> stage of reactivation, majority of patients remain HBeAg negative with positive HBeAb and their clinical presentation can be HBeAg negative chornic hepatitis < /b> B, but...

Ngày tải lên: 02/11/2012, 11:17

... clear HBV infection? Of the < /b> three antibodies against HBV, anti-HBc develops first, whereas anti-HBs antibody is detected last The < /b> reason for this sequence remains unknown The < /b> HBsAg is the < /b> most abundantly ... the < /b> anti-HBs antibody indicate recovery from infection With the < /b> cloning of the < /b> HBV genome, it became apparent that the < /b> viremia titer (number of infectious virus particles) is highest during the < /b> ... had no effect on the < /b> in vivo infectivity of the < /b> duck hepatitis < /b> B virus, but curtailed infection for the < /b> woodchuck hepatitis < /b> virus (which is more closely related to the < /b> human virus) [10, 11, 29]...

Ngày tải lên: 03/11/2012, 09:46

... trạng b nh lý phổ biến thương tổn tế b o gan Có nhiều nguyên nhân gây VG như: vi khuẩn, rượu, hóa chất vài loại thuốc vô số loại virus, có virus viêm gan B (VRVGB) [20] VRVGB hay Hepatitis < /b> B virus ... kết virus vào tế b o ký chủ; Xâm nhập virus vào tế b o (sự nhập b o); Phóng thích gen virus; Biểu sản phẩm gen virus; Sao chép gen virus; Lắp ráp hạt virion (virus hoàn chỉnh); Phóng thích virus ... virus  Gắn kết virus vào tế b o ký chủ Sự gắn kết HBV vào tế b o chủ b ớc then chốt xác định tính hướng quan virus nhờ vào điểm gắn virus thụ thể tương ứng b mặt tế b o [6] Hạt HBV hoàn chỉnh...

Ngày tải lên: 16/03/2013, 09:26

... 105 for the < /b> 1C zymogen and 13 for the < /b> 2C zymogen Overall, the < /b> disulde bond formed between the < /b> cysteine residues in the < /b> linker of the < /b> 2C zymogen seems to be detrimental to the < /b> ability of the < /b> linker ... peptide bond in natural zymogens leads to their activation by enabling the < /b> binding of substrate [38], altering the < /b> conformation of active-site residues [3], or constituting the < /b> substrate binding ... termini are labeled The < /b> sequence of the < /b> linker is given with exible residues in black, the < /b> NS5A 5B cleavage sequence in red, and the < /b> scissile bond designated with a solidus ( ) FEBS Journal 273...

Ngày tải lên: 07/03/2014, 11:20

... (Fig 2C) The < /b> solubilizing properties of NusA have already been described in the < /b> literature [29] However, it was surprising to observe MBP fusion proteins in the < /b> insoluble fractions, as the < /b> MBP carrier ... Together with the < /b> absence of consensus in the < /b> backbone carbon chemical shift differences, these observations suggest a lack of secondary structure for HCV- 1b Core+1/S This conclusion is further ... Stier G, Blandin S & de Marco A (2004) The < /b> solubility and stability of recombinant proteins are increased by their fusion to NusA Biochem Biophys Res Commun 322, 766–771 Golovanov AP, Hautbergue...

Ngày tải lên: 15/03/2014, 09:20

... BetA/CREB siCREB 100 * * CTL BetA BetA/CREB siCREB F 200 * * (Arbitrary units) 100 pCREB level by Western blot (Arbitrary units) 200 pCREB (Ser133) level by Western blot BetA/CREB 200 siCREB C BetA ... basal expression and higher accessibility to BetA On the < /b> other hand, in brain tissue, the < /b> circulating BetA can hardly gain access, owing to the < /b> protection of the < /b> blood–brain barrier, so the < /b> brain ... level by Western blot (Arbitrary units) B BetA * * CTL CREB protein level by Western blot * SOD2 mRNA level by qPCR (Arbitrary units) 150 (Arbitrary units) qPCR on SOD2 promoter by CREB ChIP...

Ngày tải lên: 16/03/2014, 01:20

... may be elevated Bile acid metabolism and HBV gene expression and not only might HBV gene expression consequently be increased, but also the < /b> anti-HBV effects of IFNs might be reduced These observations ... that the < /b> physiological regulation of HBV biosynthesis by bile acids in the < /b> liver will depend on both FXRa ⁄ JNKc-Jun pathway levels and the < /b> relative inhibition of SHP in the < /b> context of HBV gene ... with the < /b> hepatitis < /b> B virus enhancer Mol Cell Biol 9, 1804–1809 Honigwachs J, Faktor O, Dikstein R, Shaul Y & Laub O (1989) Liver-specific expression of hepatitis < /b> B virus is determined by the < /b> combined...

Ngày tải lên: 22/03/2014, 21:21

... each ISS The < /b> bold line in the < /b> box represents the < /b> median of the < /b> MFE values The < /b> box covers 50% of the < /b> MFE values The < /b> broken lines indicate MFEs inside the < /b> normal distribution, whereas the < /b> outliers ... ISSs The < /b> score was obtained by analyzing 52 HBV genomes on the < /b> RNAz webserver (http://rna.tbi.univie.ac.at/cgi-bin/RNAz.cgi) The < /b> double asterisk indicates P < 0.001 in a t-test between the < /b> HBV ... had no effect These results strongly support the < /b> conclusion that the < /b> M4 substitution compromises the < /b> silencer activity by destabilizing the < /b> S1 and S3 stems, but not by changing the < /b> sequence identity...

Ngày tải lên: 28/03/2014, 23:20