the hepatitis b virus

Báo cáo y học: "Study of the early steps of the Hepatitis B Virus life cycle"

Ngày tải lên : 03/11/2012, 10:09

... binding could be inhibited by recombinant HBs but not by the recombinant LHBs The binding of SHBs with human hepatocytes was further supported by the observation from Bruin et al [39] They have found ... of the two types [3, 4] The life cycle of HBV is believed to begin when the virus attaches to the host cell membrane via its envelope proteins Then, the viral membrane fuses with the cell membrane ... growing body of data, recently most of the published information about the early stages of hepadnavirus infection is derived from duck hepatitis B virus (DHBV), since robust DHBV infectable tissue...

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Báo cáo y học: "Identification of an effective siRNA target site and functional regulatory elements, within the hepatitis B virus posttranscriptional regulatory element" ppt

Ngày tải lên : 12/08/2014, 01:21

... calibrator = - ΔΔCt In this study, the reference gene was beta-globin, the target gene was the cccDNA of HBV co-transfected with siRNA, and the calibrator was cells transfected with only the HBV ... Inhibition of hepatitis B virus in mice by RNA interference Nat Biotechnol 2003, 21:639-644 Peng J, Zhao Y, Mai J, Pang WK, Wei X, Zhang P, Xu Y: Inhibition of hepatitis B virus replication by ... characterization of the interaction between human La and hepatitis B virus RNA J Biol Chem 2004, 279:43437-43447 Donello JE, Beeche AA, Smith GJ, Lucero GR, Hope TJ: The hepatitis B virus posttranscriptional...

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Báo cáo y học: " Prediction of conformational changes by single mutation in the hepatitis B virus surface antigen (HBsAg) identified in HBsAg-negative blood donors" ppsx

Ngày tải lên : 12/08/2014, 02:20

... mothers with HBV infection, and liver transplant recipients treated with hepatitis B immunoglobulin (HBIg) therapy [3-5] Recognition of the ’a’ determinant by antibody against HBsAg (anti-HBs) ... Virology In Hepatitis B Virus Edited by: Lai CL, Locarnini S London, International Medical Press Ltd; 2002:9-22 Weber B: Diagnostic Impact of the Genetic Variability of the Hepatitis B Virus Surface ... recognition of one of the ’a’ determinant epitopes by monoclonal anti-HBs [7] Hence, the substitution site is important because it may disturb the disulphide bonds, leading to the alteration of loop...

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Báo cáo hóa học: " Application of real-time PCR to quantify hepatitis B virus DNA in chronic carriers in The Gambia" pptx

Ngày tải lên : 20/06/2014, 01:20

... Serology Subjects were tested for HBV core antibody (anti-HBc) and if indicated HBV surface antigen (HBsAg) and HBV e antigen (HBeAg) Anti-HBc was measured by radioimmunoassay (RIA) (Sorin Biochemica, ... antigen (HBsAg) in serum HBV e antigen (HBeAg) is generally used as secondary marker to indicate high levels of virus in the blood The minority of chronic HBV carriers in whom HBeAg can be detected ... load, even before they lose HBeAg from the blood As shown in this study there is a significant decline in viral load with age in both HBeAg positive and HBeAg negative carriers Discussion The results...

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Báo cáo y học: " The characteristics of the synonymous codon usage in hepatitis B virus and the effects of host on the virus in codon usage pattern" ppsx

Ngày tải lên : 11/08/2014, 21:22

... Figure The genetic characteristic of HBV isolated different countries Based on the subtypes of HBV, the plots for the subtype adw were generally divided into two groups, while the other three subtypes ... HBV were downloaded from the National Center for Biotechnology Information (NCBI) (http://www.ncbi.nlm.nih.gov/Genbank/) and detailed information about the viruses were listed in Table Table The ... a cloned hepatitis B virus genome, subtype ayr: comparison with genomes of the other three subtypes J Gen Virol 1986, 67 ( Pt 11):2305-2314 10 Bartholomeusz A, Schaefer S: Hepatitis B virus genotypes:...

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Báo cáo y học: " Prevalence of hepatitis delta virus infection among hepatitis b virus surface antigen positive patients circulating in the largest province of pakistan" pptx

Ngày tải lên : 12/08/2014, 01:22

... to the risk factors responsible for the spread of this dual HDV/HBV infection Abbreviations HDV: hepatitis delta virus; HBV: hepatitis B virus; HCC: hepatocellular carcinoma; HBSAG: hepatitis B ... Ayub Med Coll Abbottabad 2009, 21:1-3 Seetlani NK, Abbas Z, Raza S, Yakoob J, Jafri W: Prevalence of Hepatitis D in HBsAg positive patients visiting liver clinics JPMA 2009, 59:434 Zuberi BF, ... the dual infection of HDV and HBV is available from Punjab, Pakistan that is the largest province of this country Therefore, the aim of the current study was to estimate the co-infection of hepatitis...

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Báo cáo khoa học: " Hepatitis B virus genotypes and evolutionary profiles from blood donors from the northwest region of China" docx

Ngày tải lên : 12/08/2014, 04:20

... contagiousness, as they were HBsAg, HBeAg and anti-HBc positive (Table 2) Although HBsAg in donor was negative (Table 2), HBV DNA testing proved there were few virus copies (Fig 1B) The major HBV strains ... proves the high mutation rate of HBV A DL2000 10 11 281bp 132bp 109bp Counts of samples B B C D Genotype of HBV Figure of HBV reactive blood donors Genotyping Genotyping of HBV reactive blood ... available sequences from the GenBank database were used to construct the tree with the Clustal W v1.83 program Figures in the lower part of the tree are the blood donors' numbers; characters in the...

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Báo cáo khoa học: " The role of mutations in core protein of hepatitis B virus in liver fibrosis" pdf

Ngày tải lên : 12/08/2014, 04:21

... [27] The maturation stage of the HBV has To explain the effect of mutations of core protein in the biological process of the cell, we predicted the major functions of the core protein of hepatitis ... core protein were associated with more severe liver fibrosis Abbreviations used in this paper (HBV): Hepatitis B virus; (CHB): Chronic hepatitis B; (CTL): Cytotoxic T lymphocyte Competing interests ... evaluated Twenty nine male subjects with detectable HBV DNA and candidate for liver biopsy were enrolled for the analysis of the prevalence of HBc mutations The assessed laboratory parameters were...

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Báo cáo khoa học Tạo kháng nguyên bề mặt của hbv (hepatitis b virus) bằng kỹ thuật gen và tạo kháng thể đa tròng

Ngày tải lên : 09/02/2015, 04:05

... anti-HBe xuất muộn Sự có mặt kháng thể anti-HBe tác dụng chống tái nhiễm HBV, mà biểu mắc HBV B ng Các dấu ấn thò nhiễm HBV Dấu ấn HBsAg HBeAg anti-HBs anti-HBc IgM anti-HBc IgG anti-HBe HBV DNA ... HBeAg(+) HBsAg(+) tỷ lệ lây nhiễm cao, đặc biệt phụ nữ có thai, HBsAg(+) cần theo dõi HBeAg, HBeAg(+) hầu hết họ b lây nhiễm - Kháng nguyên C (HbcAg) : HbcAg kháng nguyên lõi (core) lớp vỏ HbsAg ... thực b ớc biến nạp nhằm đưa sản phẩm nối vào chủng biểu E coli BL21(DE3) 3.7.8 Biến nạp vector tái tổ hợp vào tế b o chủ E coli a Chuẩn b tế b o khả nạp - Cấy 50µl E coli BL21(DE3) (dùng để biến...

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Báo cáo y học: "Hepatitis B Virus (HBV) and Hepatitis C Virus (HCV) Dual Infection"

Ngày tải lên : 02/11/2012, 09:51

... acute hepatitis A virus and chronic hepatitis B virus infections J Med Virol 1983;11:207-213 Pontisso P, Gerotto M, Benvegnu L, Chemello L, Alberti A Coinfection by hepatitis B virus and hepatitis ... Xing Bing Xue Za Zhi 1999 ;20:141-143 Zarski JP, Bohn B, Bastie A, Pawlotsky JM, Baud M, Bost-Bezeaux F, Tran van Nhieu J, et al Characteristics of patients with dual infection by hepatitis B and ... Interference between non-A, non -B and hepatitis B virus infection in chimpanzees J Med Virol 1983;11:191-205 Bradley DW, Maynard JE, McCaustland KA, Murphy BL, Cook EH, Ebert JW Non-A, non -B hepatitis...

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Báo cáo y học: "Enhanced surveillance for childhood hepatitis B virus infection in Canada, 1999-2003"

Ngày tải lên : 02/11/2012, 11:08

... 2005 of HBV infection was based on positive HBsAg The case definition for confirmed acute HBV infection includes the presence of HBsAg combined with IgM antibody to the HBV core (anti-HBc) A child ... Canadianborn if the child’s birthplace was reported to be in Canada, or if the date of the mother’s immigration to Canada preceded the child’s date of birth A person who did not meet these criteria ... newly diagnosed HB cases in children were reported to the EHSSS Of these, clinically recognized HB cases with neither HBsAg nor IgM anti-HBc results were excluded Thus, 195 HB cases in children...

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Báo cáo y học: "Epidemiology and Prevention of Hepatitis B Virus Infection"

Ngày tải lên : 02/11/2012, 11:12

... Southeast Asia Before HBV vaccine was integrated into the routine immunization program, the proportion of babies that become HBV carriers is about 10-30% for mothers who are HBsAg-positive but ... Quantitative analysis of HBV DNA level and HBeAg titer in hepatitis B surface antigen positive mothers and their babies: HBeAg passage through the placenta and the rate of decay in babies J Med Virol ... Occult Hepatitis B Occult hepatitis B is defined by the presence of HBV DNA in serum or liver in the absence of HBsAg [42,43] Serum HBV level is usually less than 104 copies/ml Although occult HBV...

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Báo cáo y học: "Natural History and Clinical Consequences of Hepatitis B Virus Infection"

Ngày tải lên : 02/11/2012, 11:17

... infection establishes, the serology markers like HBeAg, HBeAb and HBsAb can be positive or negative except HBsAg and HBcAb (IgG form) remain positive HBeAg positive chronic hepatitis B Age at the time ... knowledge about the HBV behavior in these individuals There is much more to be understood about mutations and their impacts on the clinical course and long-term outcome of hepatitis B infection ... as HBsAg carrier * During the stage of reactivation, majority of patients remain HBeAg negative with positive HBeAb and their clinical presentation can be HBeAg negative chornic hepatitis B, but...

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Báo cáo y học: "Hepatitis B Virus e Antigen Variants"

Ngày tải lên : 03/11/2012, 09:46

... clear HBV infection? Of the three antibodies against HBV, anti-HBc develops first, whereas anti-HBs antibody is detected last The reason for this sequence remains unknown The HBsAg is the most abundantly ... the anti-HBs antibody indicate recovery from infection With the cloning of the HBV genome, it became apparent that the viremia titer (number of infectious virus particles) is highest during the ... had no effect on the in vivo infectivity of the duck hepatitis B virus, but curtailed infection for the woodchuck hepatitis virus (which is more closely related to the human virus) [10, 11, 29]...

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XÂY DỰNG QUY TRÌNH PHÁT HIỆN ĐỘT BIẾN rtA181V/T VÀ rtN236T KHÁNG ADEFOVIR CỦA VIRUS VIÊM GAN B (Hepatitis B Virus) BẰNG KỸ THUẬT REAL-TIME PCR

Ngày tải lên : 16/03/2013, 09:26

... trạng b nh lý phổ biến thương tổn tế b o gan Có nhiều nguyên nhân gây VG như: vi khuẩn, rượu, hóa chất vài loại thuốc vô số loại virus, có virus viêm gan B (VRVGB) [20] VRVGB hay Hepatitis B virus ... kết virus vào tế b o ký chủ; Xâm nhập virus vào tế b o (sự nhập b o); Phóng thích gen virus; Biểu sản phẩm gen virus; Sao chép gen virus; Lắp ráp hạt virion (virus hoàn chỉnh); Phóng thích virus ... virus  Gắn kết virus vào tế b o ký chủ Sự gắn kết HBV vào tế b o chủ b ớc then chốt xác định tính hướng quan virus nhờ vào điểm gắn virus thụ thể tương ứng b mặt tế b o [6] Hạt HBV hoàn chỉnh...

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Báo cáo khoa học: A ribonuclease zymogen activated by the NS3 protease of the hepatitis C virus potx

Ngày tải lên : 07/03/2014, 11:20

... 105 for the 1C zymogen and 13 for the 2C zymogen Overall, the disulde bond formed between the cysteine residues in the linker of the 2C zymogen seems to be detrimental to the ability of the linker ... peptide bond in natural zymogens leads to their activation by enabling the binding of substrate [38], altering the conformation of active-site residues [3], or constituting the substrate binding ... termini are labeled The sequence of the linker is given with exible residues in black, the NS5A 5B cleavage sequence in red, and the scissile bond designated with a solidus ( ) FEBS Journal 273...

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Báo cáo khoa học: Prevalence of intrinsic disorder in the hepatitis C virus ARFP/Core+1/S protein doc

Ngày tải lên : 15/03/2014, 09:20

... (Fig 2C) The solubilizing properties of NusA have already been described in the literature [29] However, it was surprising to observe MBP fusion proteins in the insoluble fractions, as the MBP carrier ... Together with the absence of consensus in the backbone carbon chemical shift differences, these observations suggest a lack of secondary structure for HCV- 1b Core+1/S This conclusion is further ... Stier G, Blandin S & de Marco A (2004) The solubility and stability of recombinant proteins are increased by their fusion to NusA Biochem Biophys Res Commun 322, 766–771 Golovanov AP, Hautbergue...

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Báo cáo khoa học: Betulinic acid-mediated inhibitory effect on hepatitis B virus by suppression of manganese superoxide dismutase expression pot

Ngày tải lên : 16/03/2014, 01:20

... BetA/CREB siCREB 100 * * CTL BetA BetA/CREB siCREB F 200 * * (Arbitrary units) 100 pCREB level by Western blot (Arbitrary units) 200 pCREB (Ser133) level by Western blot BetA/CREB 200 siCREB C BetA ... basal expression and higher accessibility to BetA On the other hand, in brain tissue, the circulating BetA can hardly gain access, owing to the protection of the blood–brain barrier, so the brain ... level by Western blot (Arbitrary units) B BetA * * CTL CREB protein level by Western blot * SOD2 mRNA level by qPCR (Arbitrary units) 150 (Arbitrary units) qPCR on SOD2 promoter by CREB ChIP...

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Báo cáo khoa học: Bile acids increase hepatitis B virus gene expression and inhibit interferon-a activity pot

Ngày tải lên : 22/03/2014, 21:21

... may be elevated Bile acid metabolism and HBV gene expression and not only might HBV gene expression consequently be increased, but also the anti-HBV effects of IFNs might be reduced These observations ... that the physiological regulation of HBV biosynthesis by bile acids in the liver will depend on both FXRa ⁄ JNKc-Jun pathway levels and the relative inhibition of SHP in the context of HBV gene ... with the hepatitis B virus enhancer Mol Cell Biol 9, 1804–1809 Honigwachs J, Faktor O, Dikstein R, Shaul Y & Laub O (1989) Liver-speciﬁc expression of hepatitis B virus is determined by the combined...

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Báo cáo khoa học: A structured RNA in hepatitis B virus post-transcriptional regulatory element represses alternative splicing in a sequence-independent and position-dependent manner pot

Ngày tải lên : 28/03/2014, 23:20

... each ISS The bold line in the box represents the median of the MFE values The box covers 50% of the MFE values The broken lines indicate MFEs inside the normal distribution, whereas the outliers ... ISSs The score was obtained by analyzing 52 HBV genomes on the RNAz webserver (http://rna.tbi.univie.ac.at/cgi-bin/RNAz.cgi) The double asterisk indicates P < 0.001 in a t-test between the HBV ... had no effect These results strongly support the conclusion that the M4 substitution compromises the silencer activity by destabilizing the S1 and S3 stems, but not by changing the sequence identity...

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