hepatitis b virus infection of heparg cells heparg hntcp cells and primary human hepatocytes
Methods in molecular biology vol 1540 hepatitis b virus methods and protocols
... Hepatitis < /b> B Virus Infection of HepaRG Cells, HepaRG- hNTCP Cells, and Primary Human Hepatocytes Yi Ni and Stephan Urban Abstract Investigations of virus- host interactions rely on suitable in vitro ... Peng, and Wenhui Li Hepatitis < /b> B Virus Infection of HepaRG Cells, HepaRG- hNTCP Cells, and Primary Human Hepatocytes Yi Ni and Stephan Urban Live Cell Imaging Confocal ... (1997) Inducible expression of human hepatitis < /b> B virus (HBV) in stably transfected hepatoblastoma cells: a novel system for screening potential inhibitors of HBV replication Antimicrob Agents Chemother...
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Báo cáo y học: "Epidemiology and Prevention of Hepatitis B Virus Infection"
... die of HBV infection annually [4,5] Because of the high HBV-related morbidity and mortality, the global disease burden of HB is substantial Epidemiology The prevalence of chronic HBV infection ... progress HBeAg-negative CHB HBeAg-negative chronic hepatitis < /b> B (e-CHB), characterized by HBV DNA levels detectable by nonamplified assays and continued necroinflammation in the liver, has been reported ... ;142:67-71 Bancroft WH, Snitbhan R, Scott RM, Tingpalapong M, Watson WT, Tanticharoenyos P, et al Transmission of hepatitis < /b> B virus to gibbons by exposure to human saliva containing hepatitis < /b> B surface...
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Báo cáo y học: "Natural History and Clinical Consequences of Hepatitis B Virus Infection"
... chronic infection When persistent infection establishes, the serology markers like HBeAg, HBeAb and HBsAb can be positive or negative except HBsAg and HBcAb (IgG form) remain positive HBeAg positive ... with or without HBeAg seroreversion Clinical Spectrum of HBV Infection Primary Infection –Subclinical Infection and Acute hepatitis < /b> B Majority of HBV infection in children are subclinical versus ... hepatitis < /b> B virus infection Gastroenterology 1987;92:1839-43 15 Bortolotti F, Cadrobbi P, Crivellaro C, et al Long-term outcome of chronic type B hepatitis < /b> in patients who acquire hepatitis < /b> B...
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Báo cáo khoa học: "Use of dried blood samples for monitoring hepatitis B virus infection" ppsx
... virus variants circulating in Mexico List of abbreviations HBV: hepatitis < /b> B virus; DBS: dried blood spots; HBsAg: Hepatitis < /b> B surface antigen; HBeAg: Hepatitis < /b> B e antigen; HBcAg: Hepatitis < /b> B ... variation of hepatitis < /b> B surface antigen coding region among infants with chronic hepatitis < /b> B virus infection J Med Virol 2002, 68:319-327 Barbi M, Binda S, Caroppo S: Diagnosis of congenital CMV infection ... obtain, stable for long periods of time, and can be transported to a reference laboratory at minimal cost [68] Hepatitis < /b> B virus (HBV) infection is a problem in several regions of the world with limited...
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siRNA, miRNA and their possible roles in molecular therapy of chronic human hepatitis b virus infection and hepatocellular carcinoma
... HBcAg Hepatitis < /b> B core antigen HBeAg Hepatitis < /b> e antigen HBsAg Hepatitis < /b> B surface antigen HBV Hepatitis < /b> B virus HCC Hepatocellular carcinoma HCV Hepatitis < /b> C virus HIV Human immunodeficiency virus ... OF ABBREVIATIONS AFP Alpha-fetoprotein AMV Avian Myeloblastosis Virus anti-HBcAg Antibody to hepatitis < /b> B core antigen anti-HBeAg Antibody to hepatitis < /b> B e antigen anti-HBsAg Antibody to hepatitis < /b> ... to hepatitis < /b> B core antigen (anti-HBcAg) Another clinical useful antigen-antibody system that has been identified for hepatitis < /b> B is hepatitis < /b> B e antigen (HBeAg)/antibody to HBeAg (anti-HBe)...
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Báo cáo y học: "Enhanced surveillance for childhood hepatitis B virus infection in Canada, 1999-2003"
... 2005 of HBV infection was based on positive HBsAg The case definition for confirmed acute HBV infection includes the presence of HBsAg combined with IgM antibody to the HBV core (anti-HBc) A child ... it would be interpreted that the age of acquiring HBV infection between the two groups of children may exist differently In this study, it was not possible to estimate the number of HBV infections ... transmission of hepatitis < /b> B virus in Okinawa, Japan Am J Epidemiol 1988; 127: 783-787 Tables Table Rate ratio of newly identified HBV infection in children, EHSSS, 1999-2003 Variable Conflict of...
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Báo cáo khoa học: " Selective receptor expression restricts Nipah virus infection of endothelial cells" pot
... (PAEC-EB2) NiV infection of EB2 expressing PAE cells (PAECEB2) (A) PAEC and PAEC-EB2 cells were immunostained for EB2 as described in the legend to Fig 2B Magnification, ×100 (B) PAEC and PAEC-EB2 were ... 926 cells derived from human umbilical vein endothelial cells [29] As a control, Vero cells (permissive to NiV infection) and non-permissive HeLa cells were used [30] For infection studies, cells ... PAEC-EB2 cells This work was supported by grants of the German Research Foundation (DFG) to A.M (GK 1216 and SFB 593 TP B1 1) References Figure NiV infection of EB2 expressing PAE cells (PAEC-EB2)...
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Pharmacoeconomics and health outcomes research for hepatitis b virus infection in singapore
... hepatitis < /b> B virus (HBV) infection (McMahon, 1997; Lok, 2002) Symptoms of acute HBV infection are well documented but those of chronic HBV infection are less clear Little is known otherwise about ... be caused by a variety of different viruses such as hepatitis < /b> A, B, C, D and E (Roger, 2002) Of the many viral causes of human hepatitis,< /b> few are of greater global importance than hepatitis < /b> B ... function tests (LFTs) Chronic hepatitis < /b> B (CHB) HBsAg +ve with abnormal LFTs and increased HBV DNA Compensated cirrhosis (CC) Cirrhosis based on liver biopsy or obvious findings on ultrasound...
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báo cáo hóa học:" High Prevalence of Hepatitis B Virus Markers in Romanian Adolescents With Human Immunodeficiency Virus Infection" ppt
... hepatitis < /b> B core antigen (HBcAg), for antibody to hepatitis < /b> B surface antigen (HBsAg), and for antibody against HCV Sera positive for anti-HBc antibody were tested for HBsAg; sera positive for anti-HBs ... HBsAg and HBeAg All subjects without HBe antigen had undetectable levels of HBV DNA, while the HBeAg presence was accompanied by high viral load values (range, 1.54 × 107 copies/mL) Chronic HBsAg ... with hepatitis < /b> B e antigen-positive chronic hepatitis < /b> B Ann Intern Med 1993, 119:312-323 Abstract Bessesen M, Ives D, Condreay L, Lawrence S, Sherman KE: Chronic active hepatitis < /b> B exacerbations...
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Báo cáo y học: " Prevalence of hepatitis delta virus infection among hepatitis b virus surface antigen positive patients circulating in the largest province of pakistan" pptx
... Ayub Med Coll Abbottabad 2009, 21:1-3 Seetlani NK, Abbas Z, Raza S, Yakoob J, Jafri W: Prevalence of Hepatitis < /b> D in HBsAg positive patients visiting liver clinics JPMA 2009, 59:434 Zuberi BF, ... hepatitis < /b> delta virus; HBV: hepatitis < /b> B virus; HCC: hepatocellular carcinoma; HBSAG: hepatitis < /b> B surface antigen; ELISA: enzyme linked immunosorbent assay; CDNA: complementary DNA; RNA: ribonucleic ... C, Rios P, Martinez J, Alarcon Bottecchia M: Does treatment of hepatitis < /b> B virus (HBV) infection reduce hepatitis < /b> delta virus HDV replication in HIV-HBV-HDV Coinfection patients? Antivir Ther...
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Báo cáo y học: " Seropositivity of Hepatitis B virus and Hepatitis C virus dual Infection among blood donors in Nyala Teaching Hospital" pot
... occult hepatitis < /b> B virus (HBV), single occult hepatitis < /b> C virus (HCV) and occult HBV and HCV dual infection Journal of Medical Virology 2007, 79:236-241 Liu Z, Hou J: Hepatitis < /b> B virus (HBV) and hepatitis < /b> ... A: Hepatitis < /b> C virus infection in chronic hepatitis < /b> B virus carriers Journal of Infectious Diseases 1991, 163:400-402 Abou MA, Eltahir YM, Ali AS: Seroprevalence of Hepatitis < /b> B virus and Hepatitis < /b> ... receiving multiple blood transfusions Indian Journal of Gastroenterology 2002, 21:183-184 Liaw YF: Hepatitis < /b> C virus superinfection in patients with chronic Hepatitis < /b> B virus infection Journal...
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Báo cáo y học: "Hepatitis B Virus (HBV) and Hepatitis C Virus (HCV) Dual Infection"
... al Hepatitis < /b> C virus antibody and hepatitis < /b> C virus replication in chronic hepatitis < /b> B patients J Hepatol 1994;21:509-514 Liaw YF Role of hepatitis < /b> C virus in dual and triple hepatitis < /b> virus infection ... Xing Bing Xue Za Zhi 1999 ;20:141-143 Zarski JP, Bohn B, Bastie A, Pawlotsky JM, Baud M, Bost-Bezeaux F, Tran van Nhieu J, et al Characteristics of patients with dual infection by hepatitis < /b> B and ... 1983;11:191-205 Bradley DW, Maynard JE, McCaustland KA, Murphy BL, Cook EH, Ebert JW Non-A, non -B hepatitis < /b> in chimpanzees: interference with acute hepatitis < /b> A virus and chronic hepatitis < /b> B virus infections...
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Báo cáo y học: "Study of the early steps of the Hepatitis B Virus life cycle"
... SHBs was able to bind specifically to human hepatocytes, human fibroblasts and human blood mononuclear cells but not the rat hepatocytes This binding could be inhibited by recombinant HBs but ... recombinant HBs protein, they have performed binding studies with adult human hepatocytes, rat hepatocytes, human fibroblasts, human peripheral blood mononuclear cells and plasma membranes derived ... not by the recombinant LHBs The binding of SHBs with human hepatocytes was further supported by the observation from Bruin et al [39] They have found that the particles only composed by SHBs can...
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Báo cáo khoa học: Betulinic acid-mediated inhibitory effect on hepatitis B virus by suppression of manganese superoxide dismutase expression pot
... FEBS 2603 Betulinic acid inhibits hepatitis < /b> B virus D Yao et al D 200 100 * 50 100 BetA/CREB CTL E 300 (Arbitrary units) siCREB 200 * 100 * CTL BetA BetA/CREB siCREB 100 * * CTL BetA BetA/CREB ... siCREB F 200 * * (Arbitrary units) 100 pCREB level by Western blot (Arbitrary units) 200 pCREB (Ser133) level by Western blot BetA/CREB 200 siCREB C BetA 300 SOD2 protein level by Western blot ... 2009 FEBS 2607 Betulinic acid inhibits hepatitis < /b> B virus B 150 CTL BetA Enzyme activity (Arbitrary units) HBV expression (Arbitrary units) A D Yao et al PC 100 * 50 * * * * * HBsAg HBeAg HBV-DNA...
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Báo cáo khoa học: Expression studies of the core+1 protein of the hepatitis C virus 1a in mammalian cells pot
... core, b- gal and GFP are indicated by arrows colocalize with the mitotic spindle microtubules at different phases of mitosis, by double immunolabeling with anti-myc mAb and polyclonal anti-(a-tubulin) ... microtubules was also detected in interphase cells (Fig 4Ag–i, part 1) by double immunolabeling with the anti-(core+1) serum and an anti(a tubulin) mAb In addition, the protein was detected FEBS ... pHPI-1705 (B lane 2), pHPI-1706 (B lane 3) or pHPI-1748 (C lane 1) and treated with MG-132 Cell lysates were analyzed by western blotting with anti-(core+1) serum (Ba,C) or anti-core mAb (Bb) The...
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Báo cáo khoa học: Fidelity of hepatitis B virus polymerase pptx
... Seeger, C & Mason, W.S (2000) Hepatitis < /b> B virus biology Microbiol Mol Biol Rev 64, 51–68 Robinson, W.S., Clayton, D.A & Greenman, R.L (1974) DNA of a human hepatitis < /b> B virus candidate J Virol 14, ... insect cells using the recombinant baculovirus expression system Sf-9 cells were infected with the FPolE or FPolE/D551A baculovirus and were harvested 48 h postinfection From the infected cells, ... mechanism of hepatitis < /b> B virus polymerase resistance to lamivudine (3TC) and emtricitabine (FTC) J Virol 75, 4771–4779 Zoulim, F (1999) Therapy of chronic hepatitis < /b> B virus infection: inhibition of...
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Báo cáo sinh học: " Common Genotypes of Hepatitis B virus prevalent in Injecting drug abusers (addicts) of North West Frontier Province of Pakistan" doc
... transmission of major communicable diseases like Hepatitis < /b> B virus, Hepatitis < /b> C virus and Human Immunodeficiency Virus The seropositivity rate of Hepatitis < /b> B surface antigen (HBsAg) varies in the different ... genotype specific bands in patients infected with hepatitis < /b> B virus M: 50 bp marker; Lane 2–3: HBV genotype A specific 68 bp band; Lane 4–5: HBV genotype D specific 119 bp band; Lane 6–10: genotype ... of blood borne viruses such as hepatitis < /b> B and C, Ebola, Lassa virus infections and malaria [34] A simple mass-action model was applied to world census which showed that about 8– 16 million HBV,...
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Báo cáo sinh học: " Hepatitis B virus (HBV) genotypes in Egyptian pediatric cancer patients with acute and chronic active HBV infection" pptx
... markers for HBV (hepatitis < /b> B surface antigen [HBsAg], hepatitis < /b> Be antigen [HBeAg] and antibodies to hepatitis < /b> B core antigen [anti-HBc]) (enzyme immunoassay [EIA]; Adaltis, Italy) infection were ... http://www.virologyj.com/content/4/1/74 Table 1: Primer First PCR P1 S1-2 Second PCR Mix A B2 BA1R BB1R BC1R Mix B BD1 BE1 BF1 B2 R 15.70% 2.90% 10% A Sequence a(position, specificity, and polarity) 25.70% B C D 5'-TCA CCA ... Detection of HBV Precore and Core Promoter Variants J Clin Microbiol 2003, 41:3699-3705 Mizokamia M, Nakanob T, Oritob E, Tanakab Y, Sakugawac H, Mukaided M, H Robertsone B: Hepatitis < /b> B virus genotype...
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Báo cáo sinh học: " Serum levels of preS antigen (HBpreSAg) in chronic hepatitis B virus infected patients" potx
... given by the manufacture 10 11 Abbreviations HBV, hepatitis < /b> B virus; HBsAg, hepatitis < /b> B surface antigen; HBpreSAg, hepatitis < /b> B pre-surface antigen; HBeAg, hepatitis < /b> B e antigen, anti-HBs, antibody ... Table 2: Comparisons of serological markers for HBV replication versus HBVDNA copies in serum specimens HBV DNA+ HBpreSAg+ HBpreSAgn = 149 HBeAg+ HBeAg- 48 23 HBV DNA+ 43 28 n=149 HBpreSAg+ HBpreSAgn=154 ... increase of HBpreSAg binding to polyclonal anti-preS (black circle) before HBsAg could bring any interference based on the absorbance measurements using recombinant S protein Thus when it is below 20...
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Báo cáo sinh học: " Cellular apoptosis induced by replication of hepatitis B virus: possible link between viral genotype and clinical outcome" pptx
... References HBSP A BH3 HBSP B BH3 HBSP C BH3 21 31 32 35 LEEELPRLADADLHR LEEELPRLADEDLNH LEEELPRLADEGLNR Figure Alignment of BH3 domains of HBSPs Alignment of BH3 domains of HBSPs Alignment of the BH3 ... apoptotic effect by HBV genome A, B, and C Flow cytometry analysis of apoptotic effect by HBV genome A, B, and C HepG2 cells were transiently transfected with HBV genome A, B, and C and incubated for ... characterization of hepatitis < /b> B virus surface antigen mutants in Singapore patients with hepatocellular carcinoma and hepatitis < /b> B virus carriers negative for HBsAg but positive for anti-HBs and anti-HBc J Gastroenterol...
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