trans activation of growth factor receptors

... (2002) Overexpression of ganglioside GM1 results in the dispersion of platelet-derived growth factor receptor from glycolipid-enriched microdomains and in the suppression of cell growth signals J ... regulation of growth factor receptors Nat Cell Biol 4, E75–E76 Wang, X.Q., Sun, P & Paller, A.S (2002) Ganglioside induces caveolin-1 redistribution and interaction with the epidermal growth factor ... ceramide glucosyltransferase and hence of the synthesis of complex glycolipids (Fig 1, CHOK1/PPPP) [24] Exposure of cells to lM PPPP in the culture medium for days led to a 95% decrease of GM3 content...

Ngày tải lên: 16/03/2014, 16:20

... via activation of NF-jB Attenuation of LXF-289 cell proliferation by ATP is mediated by the activation of the MEK ⁄ ERK1 ⁄ 2, PI3K and p38 MAPK pathways Activation and translocation of the transcription ... proliferation of LXF-289 cells by regulation of cell cycle progression through activation of several signal transduction pathways The delay in cell cyle progression through activation of P2Y receptors ... by activation of the NF-jB1 isoform of transcription factor NF-jB, the MAPKs ERK1 ⁄ and p38 and PI3K Thus, extracellular nucleotides need to be added to the class of the critical regulators of...

Ngày tải lên: 07/03/2014, 12:20

... U/pg), proving the activation of p38 MAPK by S100A8/A9 Involvement of NF-κB activation in S100A8/A9 stimulation The transcription factor NF-κB plays a prominent role in the activation of multiple inflammatory ... value of Data were expressed as the mean value ± standard error of the Figure Detection of p38 MAPK activation To detect the activation of p38 MAPK by S100A8/A9, monocytes, at a density of × 106 ... Therefore, NF-κB activation may be essential for the transcriptional activation of many cytokine genes in monocytes stimulated by S100A8/A9 To explore the interaction of p38 MAPK with NF-κB activation...

Ngày tải lên: 09/08/2014, 07:20

... Sustained activation of the mitogen-activated protein (MAP) kinase cascade may be required for differentiation of PC12 cells Comparison of the effects of nerve growth factor and epidermal growth factor ... stimulation [5] Analysis of VEGF165-induced signal transduction in differentiated apoptotic PC12 cells demonstrated activation of ERK1/2 and Akt The transient nature of VEGF165-triggered ERK1/2 ... systems of cerebral ischemia, no comparison of VEGF and NGF activation kinetics was performed Our results on growth factor stimulated PC12 cells show that both the anti-apoptotic effect and the activation...

Ngày tải lên: 11/08/2014, 08:20

... Korsmeyer SJ & Streuli CH (2002) Activation of BAD by therapeutic inhibition of epidermal growth factor receptor and transactivation by insulin-like growth factor receptor J Biol Chem 277, 27643–27650 ... for all of the experiments PC-9 cells were plated 24 h before transfection and co-transfected with 8.5 lg of pcDL-SRa 296JNK2(VPF) or pcMKP-1 and 1.5 lg of pBabePuro by using the Lipofectamine ... the intensity of the dye is proportional to the number of the viable cells Briefly, 200 lL of a suspension of PC-9 cells was seeded into each well of a 96-well plate at a density of 2000 cellsÆwell)1...

Ngày tải lên: 18/02/2014, 13:20

... targets Transcription factors activated by ERK-PP that induce expression of genes involved in cell cycle progression include Elk1, c-fos, c-Jun and c-myc [9,15] The duration of ERK activation (transient ... of target genes expressed [16], and also affects the type of cellular response that is evoked [17,18] Activation of ERK is required for proliferation of fibroblasts [19] and constitutive ERK activation ... from the growth factor receptor complex Thus, as the local Sos concentration at the inner surface of the membrane decreases, Ras activation is impaired as well as subsequent activation of downstream...

Ngày tải lên: 19/02/2014, 16:20

... proteolytic activation of pro-hepatocyte growth factor by plasma kallikrein and coagulation factor XIa J Biol Chem 277, 47804–47809 Lee SL, Dickson RB & Lin CY (2000) Activation of hepatocyte growth factor ... (1993) Activation of the zymogen of hepatocyte growth factor activator by thrombin J Biol Chem 268, 22927–22932 Miyazawa K, Shimomura T & Kitamura N (1996) Activation of hepatocyte growth factor ... tissue injury and localized activation of HGF growth factor- binding proteins Transforming growth factor- b is produced and secreted as a latent precursor form composed of the N-terminal ‘latency-associated...

Ngày tải lên: 15/03/2014, 11:20

... Cytokine Growth Factor Rev 11, 97–102 Kim, S.J., Park, K., Rudkin, B.B., Dey, B.R., Sporn, M.B & Roberts, A.B (1994) Nerve growth factor induces transcription of transforming growth factor- b1 ... stimulation of PC12 cells results in activation of the Smad pathway independently of TGF-b1 This activation is direct and results in nuclear translocation of Smad3 within only 30 of NGF treatment ... epidermal growth factor and hepatocyte growth factor, which lead to the phosphorylation of Smad1 in the linker region and thus prevent Smad1 nuclear translocation [28] Direct effects of signaling...

Ngày tải lên: 16/03/2014, 16:20

... and activation of p38 of the SAPK2 subfamily from the sponge Suberites domuncula Biol Cell 29, 95–104 29 Laemmli, U.K (1970) Cleavage of structural proteins during the assembly of the head of ... site-directed mutagenesis of epidermal growth factor gene Biochemistry 27, 7289–7295 52 Engler, D.A., Montelione, G.T & Niyogi, S.K (1990) Human epidermal growth factor Distinct role of tyrosine 37 and ... expression level of the glucanbinding protein at the beginning of the experiments is low However, after only day of incubation in the presence of 10 lgÆmL)1 curdlan, a strong upregulation of the expression...

Ngày tải lên: 16/03/2014, 16:20

... analysis of the cDNA for a human serine protease responsible for activation of hepatocyte growth factor J Biol Chem 268, 10024–10028 Lee SL, Dickson RB & Lin CY (2000) Activation of hepatocyte growth ... Activation of the zymogen of hepatocyte growth factor activator by thrombin J Biol Chem 268, 22927–22932 11 Miyazawa K, Shimomura T & Kitamura N (1996) Activation of hepatocyte growth factor in ... by hepatocyte growth factor activator J Biol Chem 271, 3615–3618 12 Kataoka H, Hamasuna R, Itoh H, Kitamura N & Koono M (2000) Activation of hepatocyte growth factor ⁄ scatter factor in colorectal...

Ngày tải lên: 23/03/2014, 07:20

... TGF-β1 activation index: an in vitro measure of the in vivo efficiency of TGF-β1 activation To examine the ability of SLE patients and control individuals to activate TGF-β1, a ratio of levels of ... levels of TGFbeta1 act synergistically to promote activation of the vascular endothelium and formation of lipid lesions J Cell Sci 2000, 113:2355-2361 Mallat Z, Tedgui A: The role of transforming growth ... PE: Transforming growth factor beta in renal allograft recipients Transplantation 1994, 57:1727-1731 16 Coupes BM, Williams S, Roberts IS, Short CD, Brenchley PE: Plasma transforming growth factor...

Ngày tải lên: 09/08/2014, 08:22

... Relationship of epidermal growth factor receptors to airway goblet cell production Am J Respir Crit Care Med 2001, 163:1–6 Elovic A, Wong DTW, Weller PF, Matossian K, Galli SJ: Expresα sion of transforming ... T, Ueki IF, Nadel JA: Oxidative stress causes mucin synthesis via transactivation of epidermal growth factor receptor: role of neutrophils J Immunol 2000, 164:1546–1552 Blyth DI, Pedrick MS, ... Munakata M , Nasuhara Y, Sato A, Takahashi T, Homma Y, Kawakami Y: Expression of epidermal growth factor and epidermal growth factor receptor immunoreactivity in the asthmatic human airway Am J Respir...

Ngày tải lên: 12/08/2014, 18:20

... Roles of epidermal growth factor family in the regulation of postnatal somatic growth Endocr Rev 28, 284–296 Henson ES & Gibson SB (2006) Surviving cell death through epidermal growth factor ... indicate that Itch can efficiently induce tBid degradation after activation of caspase by activation of tumour necrosis factor family receptors Second, Itch also lies on the pathway activated by ... reduction of Bid expression [13] EGF treatment triggers an intricate signalling network, which leads to the activation of several kinases [34] In HEK-293T cells, EGF triggers robust activation of JNK...

Ngày tải lên: 16/02/2014, 09:20

... investigation of the increased neurotrophic activity of the AD brain revealed that it correlated with the loss of a specific neuroinhibitory factor, rather than the presence of a neurotrophic factor This factor ... Potential role of metal-binding/exchange properties of GIF in AD One of the primary pathological hallmarks of AD is the formation of b-amyloid (Ab) plaques, composed primarily of Ab(1–40) and ... survival of cultured cortical neurons [31] The neuroinhibitory action of GIF is very specific to this MT isoform and can be attributed to the small differences in the protein sequence of this isoform...

Ngày tải lên: 16/02/2014, 15:20

... activity of these FVIIa variants is presumably linked to a more stable burial of the N-terminus of the protease domain in the activation pocket of the activation domain [15] This event is (part of) ... involving Gly372 in factor VIIa E Persson and O H Olsen A B Fig Structure of FVIIa and model of G372A-FVIIa (A) Energyminimized structure of FVIIa Representation of the part of FVIIa discussed ... regulation of the cofactor-dependent serine protease coagulation factor VIIa Trends Cardiovasc Med 8, 350356 Pike ACW, Brzozowski AM, Roberts SM, Olsen OH & Persson E (1999) Structure of human factor...

Ngày tải lên: 18/02/2014, 08:20

... Activities of vascular endothelial growth factor (VEGF) family members PlGF, placenta growth factor; T.F svVEGF, Trimeresurus flavoviridis, snake-venom vascular endothelial growth factor Ligands ... region of the brain called the ‘penumbra’ [14] Therefore, degree of angiogenesis appears to correlate with rate of recovery from stroke A variety of angiogenic factors such as VEGF, fibroblast growth ... the role of VEGFR-1 signaling in angiogenesis and tumor growth in glioma, Kerber et al [27] recently studied the growth rate of intracranially transplanted glioma cells in bone marrow-transplanted...

Ngày tải lên: 18/02/2014, 11:20

... Determination of the structure of 8K-BLP MALDI-TOF MS analysis of the purified material from the final HPLC yielded a monoisotopic mass of 7409.2 ([M + H]+) (Fig 3A) Amino acid sequence analysis of the ... of the structure of 8K-BLP (A) MALDI-TOF MS analysis of the purified peptide (B) The N-terminal sequence of 8K-BLP as determined by sequence analysis X, unidentified residues (C) The sequence of ... al Members of the insulin-like peptide (ILP) family are present in a wide variety of metazoans In vertebrates, insulin and insulin-like growth factors (IGFs) regulate metabolism, growth, and...

Ngày tải lên: 18/02/2014, 13:20

... mutations of the p53 tumor suppressor gene, and overexpression of oncogenes [9,10] In addition, several studies indicate that growth factors such as nerve growth factor, insulin-like growth factor, ... a suitable signal such as growth factors, the expression of c-Fos, one of the Fos family proteins, is induced through MAP kinase activation, allowing transactivation of genes containing AP1-binding ... on mitochondria Nerve growth factor, insulin-like growth factor, and fibroblast growth factor have been reported to transmit survival signals through the phosphorylation of Bad [34] Another target...

Ngày tải lên: 19/02/2014, 06:20

... case of IGFBPrP1, because IGFBP-rP1 has a far lower affinity for IGFs than IGFBPs The high affinity binding of IGFs to IGFBPs limits the interaction of the growth factors with the cell surface receptors ... Insulin-like growth factor binding proteins Vitam Horm Rev 47, 1–114 Kim HS, Nagalla SR, Oh Y, Wilson E, Roberts CT Jr & Rosenfeld RG (1997) Identification of a family of low-affinity insulin-like growth factor ... H & Miyazaki K (1996) Synergistic growth stimulation of mouse fibroblasts by tumor-derived adhesion factor with insulin-like growth factors and insulin Cell Growth Differ 7, 1671–1677 10 Ahmed...

Ngày tải lên: 19/02/2014, 07:20

... protein levels of MAP kinases remain unchanged throughout the course of stimulation, dephosphorylation by phsophatases would be the key factor in the type of pattern of activation of the MAP kinase ... several transcription factors that play important roles in growth factor regulation of gene expression, namely AP-1, NF-jB, GATA-4 and serum response factor (SRF) [15,38] Interestingly, treatment of ... protein cofactor for these transcription factors [39] The exact mechanism by which RhoA activates these transcription factors is just beginning to be elucidated In particular, RhoA-mediated SRF activation...

Ngày tải lên: 19/02/2014, 16:20