detection of hepatocyte clones containing integrated hepatitis b virus dna using inverse nested pcr
Methods in molecular biology vol 1540 hepatitis b virus methods and protocols
... of HBV cccDNA Kristie Bloom, Abdullah Ely, and Patrick Arbuthnot Detection < /b> of Hepatocyte < /b> Clones < /b> Containing < /b> Integrated < /b> Hepatitis < /b> B Virus DNA Using Inverse Nested ... Avihepadnaviruses infecting birds Members of the Orthohepadnaviruses including WHV (Woodchuck Hepatitis < /b> virus) [1], GSHV (Ground squirrel hepatitis < /b> virus) [2], WMHBV (woolly monkey hepatitis < /b> B virus) ... is hepatitis < /b> B virus (HBV), a virus that straddles the line between DNA and RNA viruses, with a DNA genome that replicates by reverse transcription HBV and its relations in the family hepadnaviridae...
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Báo cáo y học: "Epidemiology and Prevention of Hepatitis B Virus Infection"
... characteristics, such as HBV DNA or ALT levels [41] Occult Hepatitis < /b> B Occult hepatitis < /b> B is defined by the presence of HBV DNA in serum or liver in the absence of HBsAg [42,43] Serum HBV level is usually ... progress HBeAg-negative CHB HBeAg-negative chronic hepatitis < /b> B (e-CHB), characterized by HBV DNA levels detectable by nonamplified assays and continued necroinflammation in the liver, has been reported ... Southeast Asia Before HBV vaccine was integrated < /b> into the routine immunization program, the proportion of babies that become HBV carriers is about 10-30% for mothers who are HBsAg-positive but HBeAg-negative...
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Báo cáo y học: "Natural History and Clinical Consequences of Hepatitis B Virus Infection"
... infection establishes, the serology markers like HBeAg, HBeAb and HBsAb can be positive or negative except HBsAg and HBcAb (IgG form) remain positive HBeAg positive chronic hepatitis < /b> B Age at the ... hepatitis < /b> B virus infection Gastroenterology 1987;92:1839-43 15 Bortolotti F, Cadrobbi P, Crivellaro C, et al Long-term outcome of chronic type B hepatitis < /b> in patients who acquire hepatitis < /b> B ... begins to drop With clearance of the infection, HBsAg and HBeAg disappear, and free HBsAb become detectable Presence of HBsAg, HBeAg, and high DNA level for greater than months implies progression...
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Báo cáo khoa học: "Use of dried blood samples for monitoring hepatitis B virus infection" ppsx
... virus variants circulating in Mexico List of abbreviations HBV: hepatitis < /b> B virus; DBS: dried blood spots; HBsAg: Hepatitis < /b> B surface antigen; HBeAg: Hepatitis < /b> B e antigen; HBcAg: Hepatitis < /b> B ... (Amplificasa® BIOGENICA, Mexico) PCR amplification was performed using HBV1-sense and HBV2- antisense outer primers as described previously [13] A 322 bp product was visualized by ethidium bromide ... a PCR product of 322 bp from the HBV determinant "a" genome was amplified Concordance between DBS stored at different temperatures and plasma samples for HBV -PCR amplification was optimal, because...
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siRNA, miRNA and their possible roles in molecular therapy of chronic human hepatitis b virus infection and hepatocellular carcinoma
... OF ABBREVIATIONS AFP Alpha-fetoprotein AMV Avian Myeloblastosis Virus anti-HBcAg Antibody to hepatitis < /b> B core antigen anti-HBeAg Antibody to hepatitis < /b> B e antigen anti-HBsAg Antibody to hepatitis < /b> ... HBcAg Hepatitis < /b> B core antigen HBeAg Hepatitis < /b> e antigen HBsAg Hepatitis < /b> B surface antigen HBV Hepatitis < /b> B virus HCC Hepatocellular carcinoma HCV Hepatitis < /b> C virus HIV Human immunodeficiency virus ... to hepatitis < /b> B core antigen (anti-HBcAg) Another clinical useful antigen-antibody system that has been identified for hepatitis < /b> B is hepatitis < /b> B e antigen (HBeAg)/antibody to HBeAg (anti-HBe)...
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Báo cáo y học: "Enhanced surveillance for childhood hepatitis B virus infection in Canada, 1999-2003"
... 2005 of HBV infection was based on positive HBsAg The case definition for confirmed acute HBV infection includes the presence of HBsAg combined with IgM antibody to the HBV core (anti-HBc) A child ... transmission of hepatitis < /b> B virus in Okinawa, Japan Am J Epidemiol 1988; 127: 783-787 Tables Table Rate ratio of newly identified HBV infection in children, EHSSS, 1999-2003 Variable Conflict of ... rates of residual risk for HIV, hepatitis < /b> C, hepatitis < /b> B and human Tcell lymphotropic viruses in blood donors in Canada, 1990-2000 CMAJ 2003; 169: 767-773 Duval B, Boulianne N, De Serres G, et al...
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Báo cáo khoa học: " Selective receptor expression restricts Nipah virus infection of endothelial cells" pot
... assumed to be expressed on EC in vivo, expression of EB3 in our model EC was analyzed by an EB3-specific RT -PCR [33] Fig 2C shows that besides Vero cells, only PBMEC express small amounts of EB3 mRNA ... revealed that EB3 is not only expressed in brain parenchyma but also in brain EC and might therefore be used as alternate receptor in this cell type in the absence of EB2 However, EB3 expression ... were visualized by DAPI staining Magnification, ×100 (B) mRNA was extracted from × 105 cells by standard procedures and subjected to RT -PCR with EB2-specific primers (C) RT -PCR with EB3-specific...
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Pharmacoeconomics and health outcomes research for hepatitis b virus infection in singapore
... function tests (LFTs) Chronic hepatitis < /b> B (CHB) HBsAg +ve with abnormal LFTs and increased HBV DNA Compensated cirrhosis (CC) Cirrhosis based on liver biopsy or obvious findings on ultrasound ... be caused by a variety of different viruses such as hepatitis < /b> A, B, C, D and E (Roger, 2002) Of the many viral causes of human hepatitis,< /b> few are of greater global importance than hepatitis < /b> B ... hepatitis < /b> B virus (HBV) infection (McMahon, 1997; Lok, 2002) Symptoms of acute HBV infection are well documented but those of chronic HBV infection are less clear Little is known otherwise about...
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báo cáo hóa học:" High Prevalence of Hepatitis B Virus Markers in Romanian Adolescents With Human Immunodeficiency Virus Infection" ppt
... hepatitis < /b> B core antigen (HBcAg), for antibody to hepatitis < /b> B surface antigen (HBsAg), and for antibody against HCV Sera positive for anti-HBc antibody were tested for HBsAg; sera positive for anti-HBs ... HBsAg and HBeAg All subjects without HBe antigen had undetectable levels of HBV DNA, while the HBeAg presence was accompanied by high viral load values (range, 1.54 × 107 copies/mL) Chronic HBsAg ... with hepatitis < /b> B e antigen-positive chronic hepatitis < /b> B Ann Intern Med 1993, 119:312-323 Abstract Bessesen M, Ives D, Condreay L, Lawrence S, Sherman KE: Chronic active hepatitis < /b> B exacerbations...
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Báo cáo y học: " Prevalence of hepatitis delta virus infection among hepatitis b virus surface antigen positive patients circulating in the largest province of pakistan" pptx
... factors responsible for the spread of this dual HDV/HBV infection Abbreviations HDV: hepatitis < /b> delta virus; HBV: hepatitis < /b> B virus; HCC: hepatocellular carcinoma; HBSAG: hepatitis < /b> B surface antigen; ... detection < /b> of HDV cDNA The qualitative detection < /b> of HDV-cDNA was carried out by nested PCR using four μl of synthesized HDV-cDNA The first round PCR was done in a tube containing < /b> 20 μl of PCR reaction ... of HBV and HDV co-infection using Lamivudine J Ayub Med Coll Abbottabad 2009, 21:1-3 Seetlani NK, Abbas Z, Raza S, Yakoob J, Jafri W: Prevalence of Hepatitis < /b> D in HBsAg positive patients visiting...
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Báo cáo y học: " Seropositivity of Hepatitis B virus and Hepatitis C virus dual Infection among blood donors in Nyala Teaching Hospital" pot
... occult hepatitis < /b> B virus (HBV), single occult hepatitis < /b> C virus (HCV) and occult HBV and HCV dual infection Journal of Medical Virology 2007, 79:236-241 Liu Z, Hou J: Hepatitis < /b> B virus (HBV) and hepatitis < /b> ... A: Hepatitis < /b> C virus infection in chronic hepatitis < /b> B virus carriers Journal of Infectious Diseases 1991, 163:400-402 Abou MA, Eltahir YM, Ali AS: Seroprevalence of Hepatitis < /b> B virus and Hepatitis < /b> ... approved the final manuscript References Devi KS, Singh NB, Mara J, Singh TB, Singh YM: Seroprevalence of Hepatitis < /b> B virus and Hepatitis < /b> C virus among hepatic disorders and injecting users in Manipur-A...
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Báo cáo y học: "Hepatitis B Virus (HBV) and Hepatitis C Virus (HCV) Dual Infection"
... et al Hepatitis < /b> C virus antibody and hepatitis < /b> C virus replication in chronic hepatitis < /b> B patients J Hepatol 1994;21:509-514 Liaw YF Role of hepatitis < /b> C virus in dual and triple hepatitis < /b> virus ... that anti-HCV positive, anti-HBc-positive patients who lack both HBsAg and antiHBs might be a group of patients with a multiple HBV and HCV infection HBV DNA by PCR was detected in 40.8% of 71 ... Xing Bing Xue Za Zhi 1999 ;20:141-143 Zarski JP, Bohn B, Bastie A, Pawlotsky JM, Baud M, Bost-Bezeaux F, Tran van Nhieu J, et al Characteristics of patients with dual infection by hepatitis < /b> B and...
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Báo cáo y học: "Study of the early steps of the Hepatitis B Virus life cycle"
... SHBs was able to bind specifically to human hepatocytes, human fibroblasts and human blood mononuclear cells but not the rat hepatocytes This binding could be inhibited by recombinant HBs but ... 21-33 22 Introduction The Hepatitis < /b> B virus (HBV), which belongs to the hepadnavirus family, is a small circular DNA virus containing < /b> a nucleocapsid and an envelope HBV nucleocapsid contains a ... misfolded MHBs seems to be able to interfere with the assembly of HBV virions in the persistently producing virus cell line HepG2.215 [29, 30] Anti-preS2 antibody (that recognizes MHBs) can block HBV...
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Báo cáo khoa học: Betulinic acid-mediated inhibitory effect on hepatitis B virus by suppression of manganese superoxide dismutase expression pot
... FEBS 2603 Betulinic acid inhibits hepatitis < /b> B virus D Yao et al D 200 100 * 50 100 BetA/CREB CTL E 300 (Arbitrary units) siCREB 200 * 100 * CTL BetA BetA/CREB siCREB 100 * * CTL BetA BetA/CREB ... siCREB F 200 * * (Arbitrary units) 100 pCREB level by Western blot (Arbitrary units) 200 pCREB (Ser133) level by Western blot BetA/CREB 200 siCREB C BetA 300 SOD2 protein level by Western blot ... (Arbitrary units) B BetA * * CTL CREB protein level by Western blot * SOD2 mRNA level by qPCR (Arbitrary units) 150 (Arbitrary units) qPCR on SOD2 promoter by CREB ChIP A * 100 *¶ CTL BetA BetA/CREB...
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Báo cáo khoa học: Expression studies of the core+1 protein of the hepatitis C virus 1a in mammalian cells pot
... core, b- gal and GFP are indicated by arrows colocalize with the mitotic spindle microtubules at different phases of mitosis, by double immunolabeling with anti-myc mAb and polyclonal anti-(a-tubulin) ... pHPI-1705 (B lane 2), pHPI-1706 (B lane 3) or pHPI-1748 (C lane 1) and treated with MG-132 Cell lysates were analyzed by western blotting with anti-(core+1) serum (Ba,C) or anti-core mAb (Bb) The ... polyclonal anti-(a-tubulin) and Alexa Fluor 647-conjugated goat anti(rabbit IgG) in the case of anti-myc and anti-(a-tubulin) double labeling, or with anti-(a-tubulin) mAb and Alexa Fluor 546-conjugated...
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Báo cáo khoa học: Fidelity of hepatitis B virus polymerase pptx
... Seeger, C & Mason, W.S (2000) Hepatitis < /b> B virus biology Microbiol Mol Biol Rev 64, 51–68 Robinson, W.S., Clayton, D.A & Greenman, R.L (1974) DNA of a human hepatitis < /b> B virus candidate J Virol 14, ... and extension efficiency on both RNAand DNA- templated DNA synthesis reactions by HBV DNA polymerase, given the possible role of replication infidelity in generating mutant viruses As the present study ... a PVDF blotting membrane The membranes were probed with M2 monoclonal antibody and resuspended in NaCl/Pi containing < /b> 0.5% skimmed milk and 0.3% Tween-20 The immunoblots were then incubated with...
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Báo cáo sinh học: " Common Genotypes of Hepatitis B virus prevalent in Injecting drug abusers (addicts) of North West Frontier Province of Pakistan" doc
... transmission of major communicable diseases like Hepatitis < /b> B virus, Hepatitis < /b> C virus and Human Immunodeficiency Virus The seropositivity rate of Hepatitis < /b> B surface antigen (HBsAg) varies in the different ... specific bands in patients infected 2.5% agarose hepatitis < /b> B virus 2.5% agarose gel showing genotype specific bands in patients infected with hepatitis < /b> B virus M: 50 bp marker; Lane 2–3: HBV genotype ... testing of HBV surface antigen (HBsAg) using AxSym HBsAg MEIA, Abbott Laboratories, IL, USA Molecular analysis Serological positive samples for HBsAg were further processed for HBV genotypes by the...
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Báo cáo sinh học: " Hepatitis B virus (HBV) genotypes in Egyptian pediatric cancer patients with acute and chronic active HBV infection" pptx
... number not for citation purposes) Virology Journal 2007, 4:74 http://www.virologyj.com/content/4/1/74 Table 1: Primer First PCR P1 S1-2 Second PCR Mix A B2 BA1R BB1R BC1R Mix B BD1 BE1 BF1 B2 R ... Serological markers Serologic markers for HBV (hepatitis < /b> B surface antigen [HBsAg], hepatitis < /b> Be antigen [HBeAg] and antibodies to hepatitis < /b> B core antigen [anti-HBc]) (enzyme immunoassay [EIA]; Adaltis, ... and Detection < /b> of HBV Precore and Core Promoter Variants J Clin Microbiol 2003, 41:3699-3705 Mizokamia M, Nakanob T, Oritob E, Tanakab Y, Sakugawac H, Mukaided M, H Robertsone B: Hepatitis < /b> B virus...
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Báo cáo sinh học: " Serum levels of preS antigen (HBpreSAg) in chronic hepatitis B virus infected patients" potx
... given by the manufacture 10 11 Abbreviations HBV, hepatitis < /b> B virus; HBsAg, hepatitis < /b> B surface antigen; HBpreSAg, hepatitis < /b> B pre-surface antigen; HBeAg, hepatitis < /b> B e antigen, anti-HBs, antibody ... Table 2: Comparisons of serological markers for HBV replication versus HBVDNA copies in serum specimens HBV DNA+ HBpreSAg+ HBpreSAgn = 149 HBeAg+ HBeAg- 48 23 HBV DNA+ 43 28 n=149 HBpreSAg+ HBpreSAgn=154 ... positive cases, HBpreSAg could not be detected In four HBpreSAg positive patients HBV DNA was below the detection < /b> limit by PCR For the patients showed positive in both HBpreSAg and HBV DNA, the correlation...
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Báo cáo sinh học: " Cellular apoptosis induced by replication of hepatitis B virus: possible link between viral genotype and clinical outcome" pptx
... References HBSP A BH3 HBSP B BH3 HBSP C BH3 21 31 32 35 LEEELPRLADADLHR LEEELPRLADEDLNH LEEELPRLADEGLNR Figure Alignment of BH3 domains of HBSPs Alignment of BH3 domains of HBSPs Alignment of the BH3 ... 30 incubation http://www.virologyj.com/content/4/1/117 Western blot analysis HBSP polyclonal anti-serum was acquired by boosting rabbit using an internal HBSP peptide: CDLNLGQDQQQPVRD (Biogenes, ... characterization of hepatitis < /b> B virus surface antigen mutants in Singapore patients with hepatocellular carcinoma and hepatitis < /b> B virus carriers negative for HBsAg but positive for anti-HBs and anti-HBc J Gastroenterol...
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Tìm thêm: xác định các mục tiêu của chương trình xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản khảo sát chương trình đào tạo gắn với các giáo trình cụ thể xác định thời lượng học về mặt lí thuyết và thực tế tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam nội dung cụ thể cho từng kĩ năng ở từng cấp độ xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ hệ số công suất cosp fi p2 đặc tuyến hiệu suất h fi p2 động cơ điện không đồng bộ một pha phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose