intracytoplasmic transport of hepatitis b virus capsids
Methods in molecular biology vol 1540 hepatitis b virus methods and protocols
... crosslinked membrane in a hybridization tube to perform prehybridization in 5 mL PerfectHyb™ plus hybridization buffer for 1 h at 65 °C and then overnight hybridization in 5 mL fresh hybridization buffer ... manual Cold Spring Harbor Laboratory Press, USA Chapter Intracytoplasmic < /b> Transport < /b> of Hepatitis < /b> B Virus Capsids Quentin Osseman and Michael Kann Abstract The early steps of HBV entry remain largely ... Analysis of HBV Myr-PreS1 Peptide Binding and Uptake in NTCP-GFP Expressing HepG2 Cells Alexander König and Dieter Glebe Intracytoplasmic < /b> Transport < /b> of Hepatitis < /b> B Virus Capsids ...
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Báo cáo y học: "Epidemiology and Prevention of Hepatitis B Virus Infection"
... Southeast Asia Before HBV vaccine was integrated into the routine immunization program, the proportion of babies that become HBV carriers is about 10-30% for mothers who are HBsAg-positive but HBeAg-negative ... progress HBeAg-negative CHB HBeAg-negative chronic hepatitis B (e-CHB), characterized by HBV DNA levels detectable by nonamplified assays and continued necroinflammation in the liver, has been reported ... hepatitis B virus to gibbons by exposure to human saliva containing hepatitis B surface antigen J Infect Dis 1977;135:79-85 Sevens CE, Neurath RA, Beasly RP, Szmuness W HBeAg and anti-HBe detection by...
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Báo cáo y học: "Natural History and Clinical Consequences of Hepatitis B Virus Infection"
... infection establishes, the serology markers like HBeAg, HBeAb and HBsAb can be positive or negative except HBsAg and HBcAb (IgG form) remain positive HBeAg positive chronic hepatitis B Age at the ... infection, HBsAg becomes detectable after to 10 weeks of incubation period, followed by antibodies against the core antigen (HBcAb) in IgM form during early period [2] Viremia is well established by the ... and liver begins to drop With clearance of the infection, HBsAg and HBeAg disappear, and free HBsAb become detectable Presence of HBsAg, HBeAg, and high DNA level for greater than months implies...
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Báo cáo khoa học: "Use of dried blood samples for monitoring hepatitis B virus infection" ppsx
... for the transportation of clinical plasma or serum samples, and to perform monitoring of the virus variants circulating in Mexico List of abbreviations HBV: hepatitis B virus; DBS: dried blood ... protocol Blood samples for the study and basic demographic data were obtained under informed consent from each subject (Table 1) Plasma and DBS samples Two tubes of EDTA-anticoagulated whole blood ... 6:153 Background Blood samples dried on filter paper have been successfully used to diagnose and monitor several infectious diseases The dried blood spots (DBS) have been used to detect antibodies,...
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siRNA, miRNA and their possible roles in molecular therapy of chronic human hepatitis b virus infection and hepatocellular carcinoma
... LIST OF ABBREVIATIONS AFP Alpha-fetoprotein AMV Avian Myeloblastosis Virus anti-HBcAg Antibody to hepatitis B core antigen anti-HBeAg Antibody to hepatitis B e antigen anti-HBsAg Antibody to hepatitis ... HBcAg Hepatitis B core antigen HBeAg Hepatitis e antigen HBsAg Hepatitis B surface antigen HBV Hepatitis B virus HCC Hepatocellular carcinoma HCV Hepatitis C virus HIV Human immunodeficiency virus ... hepatitis B core antigen (anti-HBcAg) Another clinical useful antigen-antibody system that has been identified for hepatitis B is hepatitis B e antigen (HBeAg)/antibody to HBeAg (anti-HBe) system...
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Báo cáo y học: "Enhanced surveillance for childhood hepatitis B virus infection in Canada, 1999-2003"
... 2005 of HBV infection was based on positive HBsAg The case definition for confirmed acute HBV infection includes the presence of HBsAg combined with IgM antibody to the HBV core (anti-HBc) A child ... Tcell lymphotropic viruses in blood donors in Canada, 1990-2000 CMAJ 2003; 169: 767-773 Duval B, Boulianne N, De Serres G, et al Should children with isolated anti-HBs or anti-HBc be immunized against ... term between age group and birthplace, and an interaction term between calendar year and birthplace on the RR scale That is, the effect of both age and calendar year depended on a child’s birthplace...
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Báo cáo khoa học: " Selective receptor expression restricts Nipah virus infection of endothelial cells" pot
... revealed that EB3 is not only expressed in brain parenchyma but also in brain EC and might therefore be used as alternate receptor in this cell type in the absence of EB2 However, EB3 expression ... SFB 593 TP B1 1) References Figure NiV infection of EB2 expressing PAE cells (PAEC-EB2) NiV infection of EB2 expressing PAE cells (PAECEB2) (A) PAEC and PAEC-EB2 cells were immunostained for EB2 ... virion binding Virology 2008, 373(1):189-201 Simmons G, Reeves JD, Grogan CC, Vandenberghe LH, Baribaud F, Whitbeck JC, Burke E, Buchmeier MJ, Soilleux EJ, Riley JL, et al.: DCSIGN and DC-SIGNR bind...
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Pharmacoeconomics and health outcomes research for hepatitis b virus infection in singapore
... 159 Table 6.4 Summary results of base-case analysis for HBeAg +ve and –ve CHB patients at years………………………………………… 166 Table 6.5 Summary results of base-case analysis for a combination of HBeAg +ve ... function tests (LFTs) Chronic hepatitis B (CHB) HBsAg +ve with abnormal LFTs and increased HBV DNA Compensated cirrhosis (CC) Cirrhosis based on liver biopsy or obvious findings on ultrasound imaging ... hepatitis B virus (HBV) infection (McMahon, 1997; Lok, 2002) Symptoms of acute HBV infection are well documented but those of chronic HBV infection are less clear Little is known otherwise about symptoms...
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báo cáo hóa học:" High Prevalence of Hepatitis B Virus Markers in Romanian Adolescents With Human Immunodeficiency Virus Infection" ppt
... hepatitis B core antigen (HBcAg), for antibody to hepatitis B surface antigen (HBsAg), and for antibody against HCV Sera positive for anti-HBc antibody were tested for HBsAg; sera positive for anti-HBs ... HBsAg and HBeAg All subjects without HBe antigen had undetectable levels of HBV DNA, while the HBeAg presence was accompanied by high viral load values (range, 1.54 × 107 copies/mL) Chronic HBsAg ... cells/mcL) Subjects of all immunologic strata were similarly exposed to HBV, as shown by the prevalence of anti-HBc antibody, but fewer adolescents with severe immunosuppression cleared HBsAg and HBeAg...
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Báo cáo y học: " Prevalence of hepatitis delta virus infection among hepatitis b virus surface antigen positive patients circulating in the largest province of pakistan" pptx
... factors responsible for the spread of this dual HDV/HBV infection Abbreviations HDV: hepatitis delta virus; HBV: hepatitis B virus; HCC: hepatocellular carcinoma; HBSAG: hepatitis B surface antigen; ... Ayub Med Coll Abbottabad 2009, 21:1-3 Seetlani NK, Abbas Z, Raza S, Yakoob J, Jafri W: Prevalence of Hepatitis D in HBsAg positive patients visiting liver clinics JPMA 2009, 59:434 Zuberi BF, ... Immediate publication on acceptance • Inclusion in PubMed, CAS, Scopus and Google Scholar • Research which is freely available for redistribution Submit your manuscript at www.biomedcentral.com/submit...
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Báo cáo y học: " Seropositivity of Hepatitis B virus and Hepatitis C virus dual Infection among blood donors in Nyala Teaching Hospital" pot
... B virus (HBV), single occult hepatitis C virus (HCV) and occult HBV and HCV dual infection Journal of Medical Virology 2007, 79:236-241 Liu Z, Hou J: Hepatitis B virus (HBV) and hepatitis C virus ... chronic hepatitis B virus carriers Journal of Infectious Diseases 1991, 163:400-402 Abou MA, Eltahir YM, Ali AS: Seroprevalence of Hepatitis B virus and Hepatitis C virus among blood donors in ... in PubMed and archived on PubMed Central yours — you keep the copyright BioMedcentral Submit your manuscript here: http://www.biomedcentral.com/info/publishing_adv.asp Page of (page number not...
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Báo cáo y học: "Hepatitis B Virus (HBV) and Hepatitis C Virus (HCV) Dual Infection"
... suggested that anti-HCV positive, anti-HBc-positive patients who lack both HBsAg and antiHBs might be a group of patients with a multiple HBV and HCV infection HBV DNA by PCR was detected in 40.8% of ... exists References 10 11 12 13 14 Table Coinfection with HBV and HCV and risk of HCC Variables Case number HCC case No HBV(-)HCV(-) HBV(+)HCV(-) HBV(-)HCV(+) HBV(+)HCV(+) 118 184 21 11.2 42.9 42.8 ... M, Wu D Study of superinfection of HBV and HCV Zhonghua Liu Xing Bing Xue Za Zhi 1999 ;20:141-143 Zarski JP, Bohn B, Bastie A, Pawlotsky JM, Baud M, Bost-Bezeaux F, Tran van Nhieu J, et al Characteristics...
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Báo cáo y học: "Study of the early steps of the Hepatitis B Virus life cycle"
... SHBs was able to bind specifically to human hepatocytes, human fibroblasts and human blood mononuclear cells but not the rat hepatocytes This binding could be inhibited by recombinant HBs but ... misfolded MHBs seems to be able to interfere with the assembly of HBV virions in the persistently producing virus cell line HepG2.215 [29, 30] Anti-preS2 antibody (that recognizes MHBs) can block HBV ... not by the recombinant LHBs The binding of SHBs with human hepatocytes was further supported by the observation from Bruin et al [39] They have found that the particles only composed by SHBs can...
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Báo cáo khoa học: Betulinic acid-mediated inhibitory effect on hepatitis B virus by suppression of manganese superoxide dismutase expression pot
... FEBS 2603 Betulinic acid inhibits hepatitis B virus D Yao et al D 200 100 * 50 100 BetA/CREB CTL E 300 (Arbitrary units) siCREB 200 * 100 * CTL BetA BetA/CREB siCREB 100 * * CTL BetA BetA/CREB ... siCREB F 200 * * (Arbitrary units) 100 pCREB level by Western blot (Arbitrary units) 200 pCREB (Ser133) level by Western blot BetA/CREB 200 siCREB C BetA 300 SOD2 protein level by Western blot ... (Arbitrary units) B BetA * * CTL CREB protein level by Western blot * SOD2 mRNA level by qPCR (Arbitrary units) 150 (Arbitrary units) qPCR on SOD2 promoter by CREB ChIP A * 100 *¶ CTL BetA BetA/CREB...
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Báo cáo khoa học: Expression studies of the core+1 protein of the hepatitis C virus 1a in mammalian cells pot
... core, b- gal and GFP are indicated by arrows colocalize with the mitotic spindle microtubules at different phases of mitosis, by double immunolabeling with anti-myc mAb and polyclonal anti-(a-tubulin) ... pHPI-1705 (B lane 2), pHPI-1706 (B lane 3) or pHPI-1748 (C lane 1) and treated with MG-132 Cell lysates were analyzed by western blotting with anti-(core+1) serum (Ba,C) or anti-core mAb (Bb) The ... polyclonal anti-(a-tubulin) and Alexa Fluor 647-conjugated goat anti(rabbit IgG) in the case of anti-myc and anti-(a-tubulin) double labeling, or with anti-(a-tubulin) mAb and Alexa Fluor 546-conjugated...
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Báo cáo khoa học: Fidelity of hepatitis B virus polymerase pptx
... C & Mason, W.S (2000) Hepatitis B virus biology Microbiol Mol Biol Rev 64, 51–68 Robinson, W.S., Clayton, D.A & Greenman, R.L (1974) DNA of a human hepatitis B virus candidate J Virol 14, 384–391 ... antibody, separated by 7.5% SDS/PAGE, and stained with Coomassie blue (A) For immunoblot analysis, proteins were electrophoretically transferred to a PVDF blotting membrane (Millipore), probed ... exact contribution of the mutations to the natural course of HBV infection remains to be elucidated, but the genetic variations of HBV are possibly related to the infidelity of the HBV polymerase...
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Báo cáo sinh học: " Common Genotypes of Hepatitis B virus prevalent in Injecting drug abusers (addicts) of North West Frontier Province of Pakistan" doc
... testing of HBV surface antigen (HBsAg) using AxSym HBsAg MEIA, Abbott Laboratories, IL, USA Molecular analysis Serological positive samples for HBsAg were further processed for HBV genotypes by the ... specific bands in patients infected 2.5% agarose hepatitis B virus 2.5% agarose gel showing genotype specific bands in patients infected with hepatitis B virus M: 50 bp marker; Lane 2–3: HBV genotype ... marker; Lane 2–3: HBV genotype A specific 68 bp band; Lane 4–5: HBV genotype D specific 119 bp band; Lane 6–10: genotype A and D specific 68 bp and 119 bp bands in patients with mixed genotype infections...
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Báo cáo sinh học: " Hepatitis B virus (HBV) genotypes in Egyptian pediatric cancer patients with acute and chronic active HBV infection" pptx
... number not for citation purposes) Virology Journal 2007, 4:74 http://www.virologyj.com/content/4/1/74 Table 1: Primer First PCR P1 S1-2 Second PCR Mix A B2 BA1R BB1R BC1R Mix B BD1 BE1 BF1 B2 R ... Serological markers Serologic markers for HBV (hepatitis B surface antigen [HBsAg], hepatitis Be antigen [HBeAg] and antibodies to hepatitis B core antigen [anti-HBc]) (enzyme immunoassay [EIA]; Adaltis, ... (1,063 bases) B2 was used as the inner primer (sense) with a combination called mix A for genotypes A, B, and C Mix A consisted of antisense primers BA1R (type A specific), BB1R (type B specific),...
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Báo cáo sinh học: " Serum levels of preS antigen (HBpreSAg) in chronic hepatitis B virus infected patients" potx
... given by the manufacture 10 11 Abbreviations HBV, hepatitis B virus; HBsAg, hepatitis B surface antigen; HBpreSAg, hepatitis B pre-surface antigen; HBeAg, hepatitis B e antigen, anti-HBs, antibody ... Table 2: Comparisons of serological markers for HBV replication versus HBVDNA copies in serum specimens HBV DNA+ HBpreSAg+ HBpreSAgn = 149 HBeAg+ HBeAg- 48 23 HBV DNA+ 43 28 n=149 HBpreSAg+ HBpreSAgn=154 ... increase of HBpreSAg binding to polyclonal anti-preS (black circle) before HBsAg could bring any interference based on the absorbance measurements using recombinant S protein Thus when it is below 20...
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Báo cáo sinh học: " Cellular apoptosis induced by replication of hepatitis B virus: possible link between viral genotype and clinical outcome" pptx
... References HBSP A BH3 HBSP B BH3 HBSP C BH3 21 31 32 35 LEEELPRLADADLHR LEEELPRLADEDLNH LEEELPRLADEGLNR Figure Alignment of BH3 domains of HBSPs Alignment of BH3 domains of HBSPs Alignment of the BH3 ... apoptotic effect by HBV genome A, B, and C Flow cytometry analysis of apoptotic effect by HBV genome A, B, and C HepG2 cells were transiently transfected with HBV genome A, B, and C and incubated for ... 30 incubation http://www.virologyj.com/content/4/1/117 Western blot analysis HBSP polyclonal anti-serum was acquired by boosting rabbit using an internal HBSP peptide: CDLNLGQDQQQPVRD (Biogenes,...
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