generation of replication competent hepatitis b virus genome from blood samples for functional characterization
Methods in molecular biology vol 1540 hepatitis b virus methods and protocols
... Quan-Xin Long, Jie-Li Hu, and Ai-Long Huang 18 Generation < /b> of Replication-< /b> Competent < /b> Hepatitis < /b> B Virus Genome from Blood Samples for Functional Characterization Yanli Qin, ... Avihepadnaviruses infecting birds Members of the Orthohepadnaviruses including WHV (Woodchuck Hepatitis < /b> virus) [1], GSHV (Ground squirrel hepatitis < /b> virus) [2], WMHBV (woolly monkey hepatitis < /b> B virus) ... crosslinked membrane in a hybridization tube to perform prehybridization in 5 mL PerfectHyb™ plus hybridization buffer for 1 h at 65 °C and then overnight hybridization in 5 mL fresh hybridization buffer...
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Báo cáo khoa học: E2A participates in a fine control of pre-mature B-cell apoptosis mediated by B-cell receptor signaling via transcriptional regulation of survivin, IAP2 and caspase-8 genes pot
... PLCc2, etc), via mainly PKCd, PKCe and PKCf, to NF-jBs (probably c-Rel and NFp50) This activated signal mediated by BCR signaling is probably transducted separately into two apoptotic pathways, ... E2A)/) is suppressed by collaboration of both BCR signaling and E2A depletion, mainly via moderate changes in amounts of the inhibitors survivin, IAP2 and ICAD (and probably FLIP) Thus, E2A is ... Reviews and Protocols in DT40 Research, (Buerstedde JM & Takeda S., eds) pp 189–205 Springer-Verlag, Berlin, Germany Riley RL, Blomberg BB & Frasca D (2005) B cells, E2A, and aging Immunol Rev 205,...
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Báo cáo khoa học: Arg143 and Lys192 of the human mast cell chymase mediate the preference for acidic amino acids in position P2¢ of substrates pdf
... (elastaselike) A large number of in vitro substrates have been identified for the chymases However, the absolute majority of these have never been shown to also be substrates in vivo [3] Therefore, ... site, which are indicated at the bottom of (A) (B, D) The cleavage of a number of substrates by HC The names and sequences of the different substrates are indicated above the pictures of the gels ... The cleavage of the same substrates as for HC in (B) and (D), but now after cleavage with the HC double mutant (F) The cleavage of a number of the above selected substrates with OC On the right...
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Báo cáo y học: "Specific eradication of HIV-1 from infected cultured cells" doc
... virus production was estimated using MAGI assay on TZM-bl cells at 48 h PI (c) Same as (a) but cells viability was estimated by the MTT assay as described in [28] (dI) and (dII) Same as (a) but ... can be seen (Fig 1c) about 40% of the cultured cells died by the eighth day PI following the addition of the Mix This percentage may represent the relative amount of virus infected cells, probably ... INS, INrs or both, a significant decrease in p24 as well as increase in apoptotic cell death could be observed (Fig 3) It should be noted that the possibility in which a low number integrated...
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Báo cáo sinh học: "The combined transduction of copper, zincsuperoxide dismutase and catalase mediated by cell-penetrating peptide, PEP-1, to protect myocardium from ischemia-reperfusion injury" ppt
... PEP-1-CAT on Bcl-2 and Bax expression Bcl-2 and Bax expression was detected by Western blot as described in Materials and Methods (A), and quantified by normalization to tubulin (B and C) Bcl-2/Bax ratio ... lyses buffer Western blot analysis was performed using procedures established in our laboratory The following antibodies were used: rabbit anti-Bax (Santa Cruz Biotechnology), mouse anti-Bcl-2 ... Bcl-2/Bax ratio while reduced Bax levels Combination of PEP-1-SOD1 and PEP-1-CAT further increased Bcl-2 level and Bcl-2/ Bax ratio, suggesting that PEP-1-SOD1 and PEP-1-CAT combination may better...
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Tài liệu Báo cáo khoa học: Properties of ecdysteroid receptors from diverse insect species in a heterologous cell culture system – a basis for screening novel insecticidal candidates docx
... VP16-USP ⁄ DDBD on MurA-inducible transcriptional activity at 2.5 lM (A) DmEcRB1 and DmEcRB2 with VP16-DmUSP and VP16-DmUSP ⁄ DDBD (B) LdEcRA and LdEcRB with VP16-LdUSP and VP16-LdUSP ⁄ DDBD (C) Cross-species ... The LdEcRB fragment was removed from pBluescriptKS + LdEcRB [31], and the vector pcDNA3.1- (Invitrogen, Carlsbad, CA, USA) was linearized by restriction digestion with XbaI and BamHI Both restriction ... unknown, although the ability of JHIII to potentiate ecdysteroid inducibility has also been observed with polychlorinated biphenyls, whose activity is associated with members of the basic helix–loop–helix...
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Báo cáo khoa học: Characterization of a second proliferating cell nuclear antigen (PCNA2) from Drosophila melanogaster docx
... was followed by dialysis in NaCl ⁄ Pi at °C for h The proteins bound to GST Sepharose- 4B beads were analyzed by western blotting with anti-T7 or anti-GST serum (B) In vivo interaction between DmPCNA2 ... three times with the same buffer, solubilized in SDS ⁄ PAGE sample buffer, and analyzed by western blotting For generation < /b> of antibodies to DNA polymerase d, the purified recombinant DNA Fractionation ... Pharmacia Biotech, Piscataway, NJ) Animals were fed water and standard rabbit food and maintained on a 12 h light/dark cycle Polyclonal antiserum to the peptide was raised in rabbits by subcutaneous...
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báo cáo hóa học:" Regulatory activity of azabisphosphonate-capped dendrimers on human CD4+ T cell proliferation enhances ex-vivo expansion of NK cells from PBMCs for immunotherapy" potx
... Malmberg KJ, Bryceson YT, Carlsten M, Andersson S, Bjorklund A, Bjorkstrom NK, Baumann BC, Fauriat C, Alici E, Dilber MS, Ljunggren HG: NK cell-mediated targeting of human cancer and possibilities ... washed twice with PBS before culture For anti-CD3/ CD28 stimulation of PBMCs or purified CD4+ T cells, 5.104 CFSE labelled cells were mixed 1.2.103 anti-CD3/ CD28 mAb-coated Dynabeads (Invitrogen) ... μg/ml in PBS and 18 μl of a RNAse solution for 30 RT and washed with PBS containing 5% fetal calf serum before flow cytometry analysis Equilibrium binding assay Cells in triplicates were incubated...
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báo cáo hóa học: " Quality of life among adolescents with sickle cell disease: mediation of pain by internalizing symptoms and parenting stress" pdf
... report of BASC anxiety, BASC depression http://www.hqlo.com/content/6/1/60 Table 2: Description of variables under study Variables Mean SD Range Parent PPQ Pain Frequency BASC Anxiety BASC Depression ... included in the model) Subsequently, where appropriate, regression models were computed to test mediation based on a procedure described by Baron and Kenny[32] as were followup Sobel's tests of the ... in SCD is not firmly established In the biobehavioral model of pain, a number of variables, in addition to disease severity, influence pain perception.[9] These variables, such as functional...
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Báo cáo khoa học " Structural characterization of immunostimulating polysaccharide from cultured mycelia of Cordyceps militaris " doc
... refractionated by gel filtration with Sepharose CL- 6B (B) Molecular weights of standard dextrans and CPMN Fr III determined by Sepharose CL- 6B gel filtration chromatography dards The data summarized in Table ... 6) -b- D-glucans Carbohydrate Research, 231, 293–307 Blumenkrantz, N., & Asboe-Hansen, G (1973) New method for quantitative determination of uronic acids Analytical Biochemistry, 54, 484–489 Bradford, ... standards (Fig 3B) 3.5 Identification of helix-coil transition A shift in the visible absorption maximum of Congo red is induced by the presence of polysaccharides and can thus be used to provide...
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Báo cáo y học: " Monocytes are essential for inhibition of synovial T-cell glucocorticoid-mediated apoptosis in rheumatoid arthritis" docx
... protein-conjugated anti-CD14 antibody (340585; BD Biosciences) and with mouse IgG1 phycoerythrin-conjugated anti-CD3 antibody (HIT3a; BD Biosciences), followed by incubation with Annexin V (TA5532; ... allophycocyanine-conjugated antiCD3 antibody (555335; BD Biosciences) and incubated with Annexin V and 7-amino-actinomycin D (7-AAD) as specified by the manufacturer (559763; BD Biosciences) and analyzed by flow cytometry ... Biotec, Bergisch Gladbach, Germany) that resulted in a cell purity of more than 90% as tested by flow cytometry with a phycoerythrin-conjugated IgG1 mouse anti-human CD3 antibody (HIT3a; BD Biosciences)...
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Báo cáo y học: "CD14 mediates the innate immune responses to arthritopathogenic peptidoglycan–polysaccharide complexes of Gram-positive bacterial cell walls" docx
... Wang J, Sartor RB, Zhang C, Bender D, Dalldorf FG, Schwab JH: Reactivation of arthritis induced by small bowel bacterial overgrowth in rats: role of cytokines, bacteria, and bacterial polymers ... mice were backcrossed to a susceptible BALB/c genetic background [22] Because mice not develop chronic arthritis, we were restricted to examination of acute arthritis, which is driven by the innate ... strongly inhibited but not abolished (Fig 6) It is possible that resident nonphagocytic cells within joints can be activated by PG-PS via CD14-independent mechanisms In this regard, Kyburz and coworkers...
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báo cáo khoa học: "A Review of Duodenal Metastases from Squamous Cell Carcinoma of the Cervix Presenting as an Upper Gastrointestinal Bleed" potx
... As the bacterial counts in the small bowel are absent or considerably lower than the large bowel, there is minimal exposure to the potentially carcinogenic chemical products of bacterial breakdown ... entire small bowel [13,14] The most common presenting symptom of small bowel lesions is a partial or complete bowel obstruction and less commonly, bowel perforation, persistent abdominal pain, ... identified by this strategy were searched with the selection of additional relevant publications for review and analysis Table provides a comprehensive review of the published cases of small bowel...
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Báo cáo khoa học: "In vitro characterization of cells derived from chordoma cell line U-CH1 following treatment with X-rays, heavy ions and chemotherapeutic drugs" pdf
... treatment of chordoma by carbon ions at our institute may be attributed to such characteristics even SOBP carbon ions are not as high RBE or low OER as monoenergetic high LET carbon beam [23,40] Conclusion ... information from the biology side because of the difficulty in obtaining basic cell biological data from the two currently available chordoma lines; the first cell line has been available for the last ... Greensboro, NC, USA U87-MG and HeLa cell lines were obtained from ATCC, USA Cells were cultured in MEM-alpha (Gibco, Japan) supplemented with 10% fetal bovine serum (FBS, Sigma, Japan) and 1% antibiotics...
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Báo cáo y học: "Monocytes are essential for inhibition of synovial T-cell glucocorticoid-mediated apoptosis in rheumatoid arthritis" pdf
... protein-conjugated anti-CD14 antibody (340585; BD Biosciences) and with mouse IgG1 phycoerythrin-conjugated anti-CD3 antibody (HIT3a; BD Biosciences), followed by incubation with Annexin V (TA5532; ... allophycocyanine-conjugated antiCD3 antibody (555335; BD Biosciences) and incubated with Annexin V and 7-amino-actinomycin D (7-AAD) as specified by the manufacturer (559763; BD Biosciences) and analyzed by flow cytometry ... Biotec, Bergisch Gladbach, Germany) that resulted in a cell purity of more than 90% as tested by flow cytometry with a phycoerythrin-conjugated IgG1 mouse anti-human CD3 antibody (HIT3a; BD Biosciences)...
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báo cáo khoa học: "Enhancive effects of Lewis y antigen on CD44-mediated adhesion and spreading of human ovarian cancer cell line RMG-I" pps
... of Biotechnology; SABC kit from Beijing Zhongshan Golden Bridge Biotechnology Co., Ltd; HA from Hefei Bomei Biotechnology Co., Ltd; DMEM culture medium from Gibco Co.; fetal bovine serum (FBS) ... anti-rabbit TRITC-labeled secondary antibody and goat anti-mouse FITC-labeled secondary antibody were diluted to 1:200 Cells were blocked by normal goat serum for 30 min, added with primary antibody ... were treated by the one-step immunofluorescence duallabeling method In brief, mouse anti-human Lewis y antibody and rabbit anti-human CD44 antibody were diluted to 1:100 as primary antibody solutions;...
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báo cáo khoa học: " Effect of filtration on morphine and particle content of injections prepared from slow-release oral morphine tablets" ppt
... particle-free, and this will be described under the combination filtration procedure Hot extraction, unfiltered The tablet could only be coarsely crushed in the crucible, but everything melted or ... appeared to be clear and melted wax could be seen floating on top The mixture was then stood on the bench until it felt cold to touch (about min) before further treatment as described for the ... mixtures was that of water to enable the volumes to be calculated Aliquots of each rinse were taken for morphine analysis Cotton ball filter A cotton ball (Home Brand Cotton Balls, Australia) was cut...
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Báo cáo y học: " Analysis of Prototype Foamy Virus particle-host cell interaction with autofluorescent retroviral particles" ppt
... stomatitis virus J Virol 2004, 78:10920-10926 35 Lampe M, Briggs JA, Endress T, Glass B, Riegelsberger S, Kräusslich HG, Lamb DC, Bräuchle C, Müller B: Double-labelled HIV-1 particles for study of virus- cell ... T, Lampe M, Briggs JA, Krausslich HG, Brauchle C, Muller B, Lamb DC: HIV-1-cellular interactions analyzed by single virus tracing Eur Biophys J 2008, 37:1291-1301 22 Jouvenet N, Bieniasz PD, ... are not released from the cell as virus- like-particles as observed for other retroviruses [reviewed in [3]] Similar to Hepatitis < /b> B virus (HBV), FV particle budding and release are instead dependent...
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Báo cáo y học: "Adoptive transfer of splenocytes to study cell-mediated immune responses in hepatitis C infection using HCV transgenic mice" pptx
... Walker BD: Broad specificity of virus- specific CD4+ T-helpercell responses in resolved hepatitis < /b> C virus infection J Virol 2002, 76:12584-12595 Radziewicz H, Ibegbu CC, Hon H, Osborn MK, Obideen ... or CD4 + (Caltag Laboratories, Hornby, ON) The cells were washed as above, fixed and permeabilized using Caltag reagent A and B fixation-permeabilization solutions (Caltag Laboratories) The cells ... were depleted of red blood cells (RBC) using RBCs lysis buffer (155 mM NH4Cl, 10 mM KHCO3, and 0.1 mM EDTA) The cellular suspension was washed three times by adding 0.1% BSA in PBS and centrifuged...
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Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn xác định thời lượng học về mặt lí thuyết và thực tế tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ hệ số công suất cosp fi p2 đặc tuyến hiệu suất h fi p2 đặc tuyến tốc độ rôto n fi p2 động cơ điện không đồng bộ một pha sự cần thiết phải đầu tư xây dựng nhà máy phần 3 giới thiệu nguyên liệu chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008 chỉ tiêu chất lượng 9 tr 25