KẾT LUẬN VÀ KIẾN NGHỊ 4.1. Kết luận
1. Tách dịng thành cơng 3 gene mã hóa con đường sinh tổng hợp vanillin từ axit ferulic, bao gồm: gltA, ech và fcs.
2. Thiết kế thành công 2 vector biểu hiện mang các gene gltA, ech và fcs là:
pRSET-GEF và pET22-GEF.
3. Đã cảm ứng biểu hiện các gen đích để sản xuất vanillin từ axit ferulic sử dụng vật chủ E. coli BL21(DE3).
4. Phân tích lđược hiệu suất sinh tổng hợp vanillin tạo thành của E. coli tái tổ hợp bằng phương pháp HPLC. Hệ thống pET22-GEF và pRSET-GEF đạt hiệu suất lần lượt là 1.44 g/L và 1.17 g/L.
4.2. Kiến nghị
Tiếp tục nghiên cứu quy trình lên men E.coli sinh tổng hợp vanillin từ axit ferulic để thu hồi vanillin thành phẩm.
Tiếp tục nghiên cứu tăng cường năng suất sinh tổng hợp vanillin theo các hướng sau:
- Bất hoạt con đường trao đổi chất cạnh tranh để dồn nguồn năng lượng và vật chất cho con đường sinh tổng hợp vanillin
- Giảm độc cho tế bào vật chủ bằng cách (1) sử dụng các chất hấp phụ; (2) chọn lọc các chủng đột biến kháng vanillin/axit ferulic
- Thiết lập các quy trình lên men hiệu quả hơn như lên men đáp ứng bề mặt hoặc lên men liên tục.
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