methods for the purification and characterization of human adipose derived stem cells

Báo cáo khoa học: A comparative analysis of the transcriptome and signal pathways in hepatic differentiation of human adipose mesenchymal stem cells doc

Ngày tải lên : 30/03/2014, 04:20

... cost and time Considerable emphasis has therefore been placed on the development of in vitro test systems Although primary human hepatocytes are the best source of cells for such systems, their ... known for 12 441 Others are unknown c The signiﬁcance of the appearance of the GO term (biological process) in the up-regulated and down-regulated genes was calculated as a P value by the software ... 0.0064 a Of the genes analyzed, the GO biological process is known for 739 up-regulated genes and 215 down-regulated genes Others are unknown b Of the genes in the mother population, the GO biological...

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Báo cáo khoa học: "Functional recovery and neural differentiation after transplantation of allogenic adipose-derived stem cells in a canine model of acute spinal cord injury" potx

Ngày tải lên : 07/08/2014, 23:22

... up to the end of the study Scores for the ASC group were significantly higher than those for the C and V groups at weeks (p

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Investigation of osteogenic characteristics of human adipose derived stromal cells

Ngày tải lên : 08/11/2015, 17:01

... damage Presently there are two well defined types of stem cells – the embryonic stem cells and the adult stem cells The embryonic stem cells, which are found within inner cell mass of the embryonic ... targeted for use in tissue regeneration are the Embryonic stem cells and the Adult stem cells The embryonic stem cells, being restricted by ethical issues regarding their use, are being sidelined and ... Lipoaspirate (PLA), adipose tissue derived progenitor cells, adipose derived stem cells etc, all denoting a lack of consensus among the taxonomists These cells are isolated from the adipose tissue...

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Báo cáo y học: " High efficient isolation and systematic identification of human adipose-derived mesenchymal stem cells." ppsx

Ngày tải lên : 10/08/2014, 10:20

... capacity of protein synthesis and nutrients uptake as reported before [24] Most cells were in resting period of cell cycle agreeing with the characteristics of human bone marrow -derived mesenchymal stem ... differentiation of cryopreserved human adipose- derived stem cells Cryobiology 2008, 57:18-24 Chen MY, Lie PC, Li ZL, Wei X: Endothelial differentiation of Wharton’s jelly -derived mesenchymal stem cells ... endothelial cells enhance osteogenesis and vascularization of mesenchymal stem cells Cells Tissues Organs 2009, 190:185-193 Rodbell M: The metabolism of isolated fat cells IV Regulation of release of...

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Characterization of human adipose tissue derived adult multipotent precursor cells

Ngày tải lên : 12/09/2015, 09:55

... Procurement of adipose tissue and their viability (Chapter 5) The two general methods of providing the suction pressure, necessary for the aspiration of adipose tissue, either through the vacuum ... volume of adipose tissue and at a faster rate Therefore this chapter sought to compare the viability and adipogenic potential of cells of the adipose tissue obtained by the pump with that by the ... regions of the skin For this study, the in vivo environment was provided at the subcutaneous region of dorsum of athymic rats There are two possible models of osteogenic induction of the cells; the...

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Báo cáo y học: " Alteration of T cell immunity by lentiviral transduction of human monocyte-derived dendritic cells" pdf

Ngày tải lên : 13/08/2014, 13:20

... added and the cells were cultured at 37°C and 5% CO2 On Day 3, ml of fresh AIM-V medium containing 560 u/ml of GM-CSF and 25 ng/ml of IL-4 was added to the culture On Day 5, the non-adherent cells ... characteristics and the percentage of positive cells and the mean fluorescence intensity (MFI) of the population were determined FACS sort of lacZ-positive cells The lentiviral siRNA vector-transduced cells ... disturb the loosely adherent cells, and ml per well of new AIM-V medium containing 560 u/ml of recombinant human GM-CSF (Research Diagnostic Inc., Flanders, NJ) and 25 ng/ml of IL-4 (R&D Systems,...

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Báo cáo y học: " Gene expression profiling of human prostate cancer stem cells reveals a pro-inflammatory phenotype and the importance of extracellular matrix interactions" pps

Ngày tải lên : 14/08/2014, 08:21

... data analysis and design SL performed the stem cell isolations from benign samples PB performed stem cell isolations from tumors CH performed culturing and isolation of stem cells, and NF-κB experiments ... (IGFBP)-4 and IGFBP-5 Proteolysis of IGFBPs regulates the bioavailability of IGFs, and because of the association between IGF levels and prostate cancer [33], strategies for the direct inhibition of ... University of Aarhus, Denmark, for his gift of antisera against the PAPPA protein and the staff of the Biology Technology Facility, University of York Apoptosis assay Unselected cells were treated for...

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Engineering and characterization of human renal proximal tubular cells for applications in vitro toxicology and bioartifical kidneys

Ngày tải lên : 09/09/2015, 10:19

... cell type-system enables the study of cells in an environment more similar to that in the human body In the kidney, for example, the endothelial cells of the peritubular capillaries and the renal ... vitro culture of primary cells (50, 54, 57-59) These different variables can have an impact on the sensitivity of the cells, and thus, thorough characterization of the cells is essential for applications ... tubular cells The functional unit of the kidney is the nephron (1) The essential parts of the nephron include the renal corpuscle (glomerulus and Bowman’ capsule), the proximal tubule, the thin and...

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ENGINEERING THREE DIMENSIONAL CULTURE PLATFORMS FOR HUMAN ADIPOSE DERIVED STEM CELL THERAPY

Ngày tải lên : 09/09/2015, 11:18

... Embryonic stem cells 2.2.2 Adult stem cells 2.2.3 Adipose derived stem cells Chapter Chapter v 2.2.4 Hepatic differentiation of adipose derived stem 10 cells 2.2.5 Characterization methods for adipogenic, ... osteogenic differentiation of 49 adipose derived stem cells 4.2.5 Hepatic differentiation of adipose derived stem 51 cells 4.2.6 Pro-angiogenic activity of adipose derived stem 53 cell – gelatin ... Injectable deliverey systems for stem cell therapy 20 2.4.1 Effect of mechanical cues on stem cells 21 2.4.2 Effect of biomolecular cues on stem cells 22 2.4.3 Hydrogels for stem cell therapy 23 2.4.4...

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Báo cáo khoa học: "Regeneration of human bones in hip osteonecrosis and human cartilage in knee osteoarthritis with autologous adipose-tissue-derived stem cells: a case series" docx

Ngày tải lên : 10/08/2014, 23:21

... proceed with stem cell treatment Before the procedure, a repeat MRI of the hip was performed and a diagnosis of osteonecrosis of the femoral head, stage 4, was confirmed He then underwent the same ... and the range of motion also further improved (Tables and 6) A post-treatment MRI taken at week 12 showed a significant increase in the thickness of meniscus cartilage on the medial side of the ... non-surgical therapy is available for the treatment of osteonecrosis and osteoarthritis Thus, stem cell therapy may significantly improve current treatment strategies for the treatment of knee osteoarthritis...

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Báo cáo sinh học: "Functional characterization of human Cd33+ And Cd11b+ myeloid-derived suppressor cell subsets induced from peripheral blood mononuclear cells co-cultured with a diverse set of human tumor cell lines" docx

Ngày tải lên : 18/06/2014, 19:20

... analyses From these investigations two major subsets of MDSC have been identified that will help elucidate the role of these cells in the ontogeny, spread, and treatment of cancer Page of 20 Methods ... by the persistence of therapeutic effects in the presence of prostaglandin E2 rescue, efficacy of analog DMC with low to absent COX inhibitory action, and the absence of effect seen with the ... important pre-clinical tool for other investigators to examine other aspects of human MDSC biology and the development of MDSCdirected therapies Furthermore, from these analyses two simplified...

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Báo cáo y học: "Identification and isolation of embryonic stem cells in reproductive endocrinology: theoretical protocols for conservation of human embryos derived from in vitro fertilization" ppsx

Ngày tải lên : 13/08/2014, 23:20

... presents a substantial ethical obstacle for the advancement of human ESC research With the vast therapeutic promise of human ESC seen against the destruction of human embryos required to realize such ... obtained for further analysis Refinement and increased efficiency of these two ESC protocols brings the potential to offer a reliable supply of embryonic stem cells without production of whole ... effective methods to verify stemness and monitor quality in such cells are also needed to bring the full range of therapeutic possibility into focus In an effort to develop an assessment system for...

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Immunological characterization of human umbilical cord lining derived cells and their therapeutic application in a diabetic mouse model

Ngày tải lên : 11/09/2015, 10:02

... fetal/neonatal and adult I will review first embryonic stem cells, induced pluripotent stem cells and then the adult bone marrow derived stem cells, which are well known and best studied for their immune ... either for stem transplantation or for supportive immune modulation during other types of therapies The current challenges still lie with the understanding of the underlying mechanisms and the ... The uNK cells are members of the maternal immune system The EVTs are derived from the trophectoderm of the embryo and invade into the decidualised uterus during placentation These two types of...

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báo cáo hóa học:" Human fallopian tube: a new source of multipotent adult mesenchymal stem cells discarded in surgical procedures" potx

Ngày tải lên : 18/06/2014, 15:20

... research group in the identification, and characterization of potential sources of adult stem cells [7,10,1215], the aim of this study was to isolate, expand, characterize and assess the differentiation ... integrity of the tubal epithelium are of paramount importance for the development of a unique microenvironment required for optimal fertilization and early embryo development They are therefore essential ... chondrogenic, and osteogenic tissues in vitro (figure 4) Together, these results confirmed the mesenchymal nature of the isolated cells and their multipotency Page of 10 (page number not for citation...

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Báo cáo y học: "Human infrapatellar fat pad-derived stem cells express the pericyte marker 3G5 and show enhanced chondrogenesis after expansion in fibroblast growth factor-2" potx

Ngày tải lên : 09/08/2014, 10:23

... staining of IPFPderived cells It was therefore interesting that they showed a pattern of expression on a high proportion of the IPFP -derived cells and of epitopes commonly abundant on adult stem cells ... stem cells [48] FGF2 is routinely used in the culture of bone marrow -derived mesenchymal stem cells, and we have determined a baseline for the use of FGF-2 in the culture of fat pad -derived cells ... enhanced for cells expanded in the presence of FGF-2 Although cell aggregates derived from cells expanded in the presence of FGF-2 stained for collagen type I, the immunostaining was increased at the...

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Báo cáo y học: "Isolation and characterization of human cells resistant to retrovirus infection" pdf

Ngày tải lên : 13/08/2014, 05:22

... infection in cells of differing types and species [11,12] Therefore, to build upon and extend the rodent cell studies, and to identify cellular factors in human cells required for the early phase of infection ... and mutant cells and extent of HIV Growth Growth rates of parental and mutant cells and extent of HIV integration (A) Growth rates Parental and mutant 30-2 and 42-7 cells were seeded and growth ... fluorescence microscope and comparing cell clones to wild-type cells and to each other Two clones (30 and 42) were chosen for further analysis on the basis of their resistance to infection and growth rates...

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Báo cáo y học: "Characterization of Human Erythrocytes as Potential Carrier for Pravastatin: An In Vitro Study"

Ngày tải lên : 25/10/2012, 11:10

... administration of statins with certain juices [6] or inhibition of Pgp [3] Unfortunately these strategies are frequently associated with increase the risk of side effects of the statins [3,7].Therefore the ... recovery The percentage ratio of the hematocrite value of the final loaded cells to that of the initial packed cells, both measured using equal suspension volumes In vitro characterization of pravastatin ... entrapped in 0.1 ml of the final packed erythrocytes Efficiency of entrapment The percentage of the loaded amount of pravastatin to the total amount of that added during the entire loading process...

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Tài liệu Báo cáo khoa học: Tissue expression and biochemical characterization of human 2-amino 3-carboxymuconate 6-semialdehyde decarboxylase, a key enzyme in tryptophan catabolism pptx

Ngày tải lên : 19/02/2014, 02:20

... alignment of human and bacterial ACMSD and the comparison of their three-dimensional structures, the presence of the metallocofactor in the human enzyme can be reliably predicted Indeed, in the present ... probe set in the presence of increasing molar amounts of the other isoform (1 : 1, : 1000 and : 10 000) Detection of ACMSD I was not inﬂuenced by the presence of up to Recombinant human ACMSD ... on human ACMSD The presence of the metallocofactor in human ACMSD might also be inferred by our study on the enzyme pH dependence, showing that the increase of the concentrations of succinate and...

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Tài liệu Báo cáo khoa học: Characterization of human deoxyribonuclease I gene (DNASE1) promoters reveals the utilization of two transcription-starting exons and the involvement of Sp1 in its transcriptional regulation ppt

Ngày tải lên : 19/02/2014, 06:20

... and quantitative real-time RT-PCR, the locations of the primers DN)105 and DN)9 used in ChIP assays, the locations of the primers DN+232 and DN+254 utilized in 5¢-RACE, and the locations of the ... coding sequence of the human DNASE1 message by using an Expanded High Fidelity PCR system (Roche) with the total RNA from QGP-1 cells and a primer set of the forward primer DN-5R and the reverse ... only the 5¢-UTR of the mRNA [15,16] However, comparison of the sequence of the 5¢-UTR of human pancreatic DNASE1 mRNA reported previously [15] with the human genomic sequence showed that the 5¢-terminal...

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Tài liệu Báo cáo khoa học: Crystal structure of an ascomycete fungal laccase from Thielavia arenaria – common structural features of asco-laccases ppt

Ngày tải lên : 14/02/2014, 18:20

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