differentiation of human adipose derived stem cells into cardiomyocytes

... evidence of transdifferentiation by MET in the process of hepatic differentiation of AT-MSCs No previous report has demonstrated evidence of transdifferentiation of committed adult stem cells In ... vitro differentiation of human umbilical cord blood -derived mesenchymal stem cells into hepatocytelike cells Biochem Biophys Res Commun 330, 1153–1161 Seo MJ, Suh SY, Bae YC & Jung JS (2005) Differentiation ... Isolation of mesenchymal stem cells of fetal or maternal origin from human placenta Stem Cells 22, 1338–1345 Bieback K, Kern S, Kluter H & Eichler H (2004) Critical parameters for the isolation of mesenchymal...

Ngày tải lên: 30/03/2014, 04:20

... Immunophenotype of human adiposederived cells: temporal changes in stromal-associated and stem cell-associated markers Stem Cells 2006, 24, 376-385 26 Muir WW 3rd, Wiese AJ, March PA Effects of morphine, ... HE Stem cell therapy for neurologic disorders: therapeutic potential of adipose- derived stem cells Curr Drug Targets 2005, 6, 57-62 33 Satake K, Lou J, Lenke LG Migration of mesenchymal stem cells ... with isoflurane Am J Vet Res 2003, 64, 1155-1160 27 Ogawa R The importance of adipose- derived stem cells and vascularized tissue regeneration in the field of tissue transplantation Curr Stem Cell...

Ngày tải lên: 07/08/2014, 23:22

... there are two well defined types of stem cells – the embryonic stem cells and the adult stem cells The embryonic stem cells, which are found within inner cell mass of the embryonic blastocyst, is ... good source of multipotent adult stem cells is the adipose tissue Cells isolated from these, known as ADSCs (Adipose Derived Stromal Cells) have been shown to possess the property of being multipotent ... of stem cells and precursor cells for this purpose The two types of stem cells that are being targeted for use in tissue regeneration are the Embryonic stem cells and the Adult stem cells The...

Ngày tải lên: 08/11/2015, 17:01

... triggers differentiation of HUMSCs into cardiomyocytes and maturation of HUMSCs derived cardiomyocytes Admittedly, the detailed mechanism(s) of above effects of S1P on differentiation of and maturation ... Review: Human Umbilical Cord Stroma with Regard to the Source of Fetus -Derived Stem Cells Stem cells 2007, 25:2886-2895 Wu KH, Mo XM, Zhou B, Lu SH, Yang SG, Liu YL, Han ZC: Cardiac potential of stem ... cardiac myocyte and cardiomyocytes sheets from differentiated human umbilical cord mesenchymal stem cells We assessed viability of the cells of sheets at room temperature No indication of cell apoptosis...

Ngày tải lên: 10/08/2014, 05:21

... cryopreserved human adipose- derived stem cells Cryobiology 2008, 57:18-24 Chen MY, Lie PC, Li ZL, Wei X: Endothelial differentiation of Wharton’s jelly -derived mesenchymal stem cells in comparison ... DR: Multilineage potential of adult human mesenchymal stem cells Science 1999, 284:143-147 Pittenger MF, Mosca JD, McIntosh KR: Human mesenchymal stem cells: progenitor cells for cartilage, bone, ... endothelial cells enhance osteogenesis and vascularization of mesenchymal stem cells Cells Tissues Organs 2009, 190:185-193 Rodbell M: The metabolism of isolated fat cells IV Regulation of release of...

Ngày tải lên: 10/08/2014, 10:20

... function of DCs Polarization of naïve Th cells into Th1 cells is critical for the induction of cellular immunity against intracellular pathogens and cancer cells The observed impairment of the ... dilutions of DCs, from 10,000 cells per well to 313 cells per well, with × 105 allogeneic CD4 T cells in a 96-well U-bottomed plate in a total volume of 200 µl for days The proliferation of T cells ... expression profile of DCs, we suspected that LV infection might also affect DC activation of T cells To test this, we set up an in vitro immunity assay using co-culture of human DCs and naïve T cells...

Ngày tải lên: 13/08/2014, 13:20

... in human prostate cancer, defined by the phenotype CD133+/α2β1hi (high expression of α2β1 integrin) and comprising less than 0.1% of the tumor mass, has many of the properties of cancer stem cells ... 2008, Volume 9, Issue 5, Article R83 (b) Differentiation signature Combined signature Stem cells Stem cells (c) Committed basal Committed basal Stem Committed cells basal (d) Malignancy signature ... cancer cells PTL induces apoptosis in primitive cancer cells Percent viability of prostate cancer cells and cells from a patient with BPH treated with increasing concentrations of PTL Cells were...

Ngày tải lên: 14/08/2014, 08:21

... 2.2 Stem cells in tissue regeneration 2.2.1 Embryonic stem cells 2.2.2 Adult stem cells 2.2.3 Adipose derived stem cells Chapter Chapter v 2.2.4 Hepatic differentiation of adipose derived stem ... of stemness marker genes on gelatin 49 microspheres 4.2.4 Adipogenic and osteogenic differentiation of 49 adipose derived stem cells 4.2.5 Hepatic differentiation of adipose derived stem 51 cells ... Characterization of mechanical properties of 63 Col-GM scaffolds 5.2.2 Adipose derived stem cell culture in Col-GM 65 scaffolds 5.2.3 Osteogenic differentiation of adipose derived 67 stem cells in Col-GM...

Ngày tải lên: 09/09/2015, 11:18

... vitro differentiation of size-sieved stem cells into electrically active neural cells Stem Cells 2002, 20(6):522-529 Tzeng SF, Tsai MJ, Hung SC, Cheng H: Neuronal morphological change of size-sieved ... infected cells for 48 hours Results FN and LAM enhance differentiation of MSCs into insulin producing cells To examine the effects of ECM, FN and LAM on IPC differentiation by MSCs, a four-stage differentiation ... H, Fishman B, Ziskind A, Shulman M, Itskovitz-Eldor J: Differentiation of human embryonic stem cells into insulin-producing clusters Stem Cells 2004, 22(3):265-274 Chang CF, Hsu KH, Chiou SH,...

Ngày tải lên: 10/08/2014, 05:21

... approximately 40 cm3 of packed adipose tissue, fibrous tissue, red blood cells and a small number of nucleated cells An equal volume of digestive enzyme, 0.07% collagenase type 1, composed of several collagenases, ... mechanism of regeneration is not yet clear It could be through direct differentiation of stem cells that were introduced to the diseased joints Alternatively, it could be due to the tropic effects of ... Mesenchymal stem cell therapy in joint disease Novartis Found Symp 2003, 249:86-96 Zhang HN, Li L, Leng P, Wang YZ, Lv CY: Uninduced adipose- derived stem cells repair the defect of full-thickness...

Ngày tải lên: 10/08/2014, 23:21

... evaluate adipogenic potential of amniotic fluid -derived human mesenchymal stem cells Stem Cells Dev 2006, 15:719-726 55 Montaln H, Schichor C, Lah TT: Human mesenchymal stem cells and their use in cell-based ... infection of human bone marrow stromal fibroblasts Blood 1990, 76:317-322 40 Wang L, Mondal D, La Russa VF, Agrawal KC: Suppression of clonogenic potential of human bone marrow mesenchymal stem cells ... 0.05 smooth muscle and endothelial cells To study the effects of HIV-1 on the differentiation of these cells, the interaction of HIV-1 and recombinant gp120 on MSC differentiation to adipogenic and...

Ngày tải lên: 13/08/2014, 01:20

... stem cells (mESCs) can be derived and manipulated has made them an ideal model system for the study of developmental biology In vitro differentiation of mESCs has successfully given rise to cells ... stimulation of regulatory T (Treg) cells in the host This mechanism in part can be attributed to the differentiation of the mixed pool of donor haematopoietic cells into allogeneic antigen-presenting cells ... analysis of developmental events was made possible with the isolation of embryonic stem cells Extensive investigation of the properties and capabilities of these cells has led to the development of...

Ngày tải lên: 04/10/2015, 16:03

... expression pattern of undifferentiated and differentiated human embryonic stem cells Stem Cells Dev 2007, 16(6):1003-1016 Page 15 of 17 (page number not for citation purposes) Journal of Translational ... The tested adult cells were Human Microvascular Endothelial Cells (HMVEC), Human Umbilical Vein Endothelial Cells (HUVEC), Umbilical Artery Smooth Muscle Cells (UASMC), Normal human astrocytes ... 13 The expression of miR-92 has been reported in human embryonic stem (ES) cells [16,26], mouse ES cells[ 20] or Page 12 of 17 (page number not for citation purposes) Journal of Translational Medicine...

Ngày tải lên: 18/06/2014, 15:20

... number of cells was determined spectrophotometrically entiation of human mesenchymal transcripts Expression of BMP-2 and chordinstem cells during osteogenic differentiation of human mesenchymal stem ... Lecanda F, Avioli LV, Cheng SL: Regulation of bone matrix protein expression and induction of differentiation of human osteoblasts and human bone marrow stromal cells by bone morphogenetic protein-2 ... Kassem M: Inhibition of osteoblast differentiation but not adipocyte differentiation of mesenchymal stem cells by sera obtained from aged females Bone 2006, 39:181-188 Page of (page number not...

Ngày tải lên: 09/08/2014, 10:23

... mesenchymal stem cells for osteoarthritis therapy Nat Clin Pract Rheumatol 2008, 4:371-380 Estes BT, Wu AW, Guilak F: Potent induction of chondrocytic differentiation of human adipose- derived adult stem ... Chondrogenic differentiation of cultured human mesenchymal stem cells from marrow Tissue Eng 1998, 4:415-428 35 Mueller MB, Tuan RS: Functional characterization of hypertrophy in chondrogenesis of human ... arcades of Benninghoff [29] and by the appearance of hypertrophic cells Analyses of lacunae surface areas in the different growth plate zones revealed mean lacunae surface areas ± SD of 100.8...

Ngày tải lên: 09/08/2014, 14:22

... transfer [2] Homogenous human ESC colonies may be derived from subsequent isolation and re-plating of the ICM cells, which are then screened for stemness by a variety of recognized markers Once ... substantial ethical obstacle for the advancement of human ESC research With the vast therapeutic promise of human ESC seen against the destruction of human embryos required to realize such aspirations, ... stage, a human embryo at 2–3d post fertilization consists of just 4–8 cells, all of which are totipotent In contrast to the pluripotent cells obtained from the blastocyst ICM, one or two of these...

Ngày tải lên: 13/08/2014, 23:20

... NANOFIBERS FOR OSTEOGENIC DIFFERENTIATION OF HUMAN MESENCHYMAL STEM CELLS NGUYEN THI HIEN LUONG (B.Eng., Ho Chi Minh city University of Technology, Vietnam) A THESIS SUBMITTED FOR THE DEGREE OF ... be loaded into nanofibrous scaffolds by electrospinning, and the sustained release of this drug can induce the osteogenic differentiation of MSCs e) Incorporation of Type I Col into nanofibrous ... the osteogenic differentiation of MSCs investigate the influences of prolonged culture as well as the nanofibrous structure on osteogenic differentiation function of the stem cells To produce...

Ngày tải lên: 09/09/2015, 10:07

... osteogenic differentiation of human mesenchymal stem cells Exp Cell Res (1):22-37 101 Salasznyk RM, Klees RF, Boskey A, et al 2007;Activation of FAK is necessary for the osteogenic differentiation of human ... differentiation of rat bone marrow -derived mesenchymal stem cells J Bone Miner Res (10):16061614 59 Matsubara T, Tsutsumi S, Pan H, et al 2004;A new technique to expand human mesenchymal stem cells using ... 2009;Mesenchymal stem cell modification of endothelial matrix regulates their vascular differentiation J Cell Biochem (4):706-713 104 Kuhn NZ, Tuan RS 2010;Regulation of stemness and stem cell niche of mesenchymal...

Ngày tải lên: 11/09/2015, 09:16

... Casey K Chan Effects of mechanical stimulation in osteogenic and chondrogenic differentiation of bone marrow -derived mesenchymal stem cells on nanofibrous scaffolds Journal of Bioactive and Compactable ... Types of cells used in bone tissue engineering for osteogenic differentiation 59 2.3.1 Potential of mesenchymal stem cells (MSCs) for bone healing 60 2.3.2 Potential of bone marrow derived ... 2.3.2 Potential of bone marrow derived mesenchymal stem cells (BM-MSCs) 61 2.3.3 Potential of adipose- derived stem cells 63 2.3.4 MSCs derived from other sources 64 2.4 Cell-material...

Ngày tải lên: 11/09/2015, 09:57

... CHARACTERIZATION OF HUMAN ADIPOSE DERIVED ADULT MULTIPOTENT PRECURSOR CELLS LEONG TAI WEI DAVID (Bachelor of Chemical Engineering, Hons) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY DEPARTMENT OF ... characterization of other potential sources of stem cells which might avoid these issues altogether 1.1.2.1 Adult stem cells Stem cells are loosely defined as self-renewing progenitor cells that can ... that cells actually thrive in One of the promising adult stem cell sources is a culture plastic-adherent cell population from the human adipose tissue In order to bring adipose derived stem cells...

Ngày tải lên: 12/09/2015, 09:55