... evidence of transdifferentiation by MET in the process of hepatic differentiationof AT-MSCs No previous report has demonstrated evidence of transdifferentiation of committed adult stemcells In ... vitro differentiationofhuman umbilical cord blood -derived mesenchymal stemcellsinto hepatocytelike cells Biochem Biophys Res Commun 330, 1153–1161 Seo MJ, Suh SY, Bae YC & Jung JS (2005) Differentiation ... Isolation of mesenchymal stemcellsof fetal or maternal origin from human placenta StemCells 22, 1338–1345 Bieback K, Kern S, Kluter H & Eichler H (2004) Critical parameters for the isolation of mesenchymal...
... Immunophenotype ofhuman adiposederived cells: temporal changes in stromal-associated and stem cell-associated markers StemCells 2006, 24, 376-385 26 Muir WW 3rd, Wiese AJ, March PA Effects of morphine, ... HE Stem cell therapy for neurologic disorders: therapeutic potential of adipose- derivedstemcells Curr Drug Targets 2005, 6, 57-62 33 Satake K, Lou J, Lenke LG Migration of mesenchymal stemcells ... with isoflurane Am J Vet Res 2003, 64, 1155-1160 27 Ogawa R The importance of adipose- derivedstemcells and vascularized tissue regeneration in the field of tissue transplantation Curr Stem Cell...
... there are two well defined types ofstemcells – the embryonic stemcells and the adult stemcells The embryonic stem cells, which are found within inner cell mass of the embryonic blastocyst, is ... good source of multipotent adult stemcells is the adipose tissue Cells isolated from these, known as ADSCs (Adipose Derived Stromal Cells) have been shown to possess the property of being multipotent ... ofstemcells and precursor cells for this purpose The two types ofstemcells that are being targeted for use in tissue regeneration are the Embryonic stemcells and the Adult stemcells The...
... triggers differentiationof HUMSCs intocardiomyocytes and maturation of HUMSCs derivedcardiomyocytes Admittedly, the detailed mechanism(s) of above effects of S1P on differentiationof and maturation ... Review: Human Umbilical Cord Stroma with Regard to the Source of Fetus -Derived StemCellsStemcells 2007, 25:2886-2895 Wu KH, Mo XM, Zhou B, Lu SH, Yang SG, Liu YL, Han ZC: Cardiac potential ofstem ... cardiac myocyte and cardiomyocytes sheets from differentiated human umbilical cord mesenchymal stemcells We assessed viability of the cellsof sheets at room temperature No indication of cell apoptosis...
... function of DCs Polarization of naïve Th cellsinto Th1 cells is critical for the induction of cellular immunity against intracellular pathogens and cancer cells The observed impairment of the ... dilutions of DCs, from 10,000 cells per well to 313 cells per well, with × 105 allogeneic CD4 T cells in a 96-well U-bottomed plate in a total volume of 200 µl for days The proliferation of T cells ... expression profile of DCs, we suspected that LV infection might also affect DC activation of T cells To test this, we set up an in vitro immunity assay using co-culture ofhuman DCs and naïve T cells...
... in human prostate cancer, defined by the phenotype CD133+/α2β1hi (high expression of α2β1 integrin) and comprising less than 0.1% of the tumor mass, has many of the properties of cancer stemcells ... 2008, Volume 9, Issue 5, Article R83 (b) Differentiation signature Combined signature StemcellsStemcells (c) Committed basal Committed basal Stem Committed cells basal (d) Malignancy signature ... cancer cells PTL induces apoptosis in primitive cancer cells Percent viability of prostate cancer cells and cells from a patient with BPH treated with increasing concentrations of PTL Cells were...
... approximately 40 cm3 of packed adipose tissue, fibrous tissue, red blood cells and a small number of nucleated cells An equal volume of digestive enzyme, 0.07% collagenase type 1, composed of several collagenases, ... mechanism of regeneration is not yet clear It could be through direct differentiationofstemcells that were introduced to the diseased joints Alternatively, it could be due to the tropic effects of ... Mesenchymal stem cell therapy in joint disease Novartis Found Symp 2003, 249:86-96 Zhang HN, Li L, Leng P, Wang YZ, Lv CY: Uninduced adipose- derivedstemcells repair the defect of full-thickness...
... evaluate adipogenic potential of amniotic fluid -derived human mesenchymal stemcellsStemCells Dev 2006, 15:719-726 55 Montaln H, Schichor C, Lah TT: Human mesenchymal stemcells and their use in cell-based ... infection ofhuman bone marrow stromal fibroblasts Blood 1990, 76:317-322 40 Wang L, Mondal D, La Russa VF, Agrawal KC: Suppression of clonogenic potential ofhuman bone marrow mesenchymal stemcells ... 0.05 smooth muscle and endothelial cells To study the effects of HIV-1 on the differentiationof these cells, the interaction of HIV-1 and recombinant gp120 on MSC differentiation to adipogenic and...
... stemcells (mESCs) can be derived and manipulated has made them an ideal model system for the study of developmental biology In vitro differentiationof mESCs has successfully given rise to cells ... stimulation of regulatory T (Treg) cells in the host This mechanism in part can be attributed to the differentiationof the mixed pool of donor haematopoietic cellsinto allogeneic antigen-presenting cells ... analysis of developmental events was made possible with the isolation of embryonic stemcells Extensive investigation of the properties and capabilities of these cells has led to the development of...
... expression pattern of undifferentiated and differentiated human embryonic stemcellsStemCells Dev 2007, 16(6):1003-1016 Page 15 of 17 (page number not for citation purposes) Journal of Translational ... The tested adult cells were Human Microvascular Endothelial Cells (HMVEC), Human Umbilical Vein Endothelial Cells (HUVEC), Umbilical Artery Smooth Muscle Cells (UASMC), Normal human astrocytes ... 13 The expression of miR-92 has been reported in human embryonic stem (ES) cells [16,26], mouse ES cells[ 20] or Page 12 of 17 (page number not for citation purposes) Journal of Translational Medicine...
... number ofcells was determined spectrophotometrically entiation ofhuman mesenchymal transcripts Expression of BMP-2 and chordinstem cells during osteogenic differentiationofhuman mesenchymal stem ... Lecanda F, Avioli LV, Cheng SL: Regulation of bone matrix protein expression and induction ofdifferentiationofhuman osteoblasts and human bone marrow stromal cells by bone morphogenetic protein-2 ... Kassem M: Inhibition of osteoblast differentiation but not adipocyte differentiationof mesenchymal stemcells by sera obtained from aged females Bone 2006, 39:181-188 Page of (page number not...
... mesenchymal stemcells for osteoarthritis therapy Nat Clin Pract Rheumatol 2008, 4:371-380 Estes BT, Wu AW, Guilak F: Potent induction of chondrocytic differentiationofhuman adipose- derived adult stem ... Chondrogenic differentiationof cultured human mesenchymal stemcells from marrow Tissue Eng 1998, 4:415-428 35 Mueller MB, Tuan RS: Functional characterization of hypertrophy in chondrogenesis ofhuman ... arcades of Benninghoff [29] and by the appearance of hypertrophic cells Analyses of lacunae surface areas in the different growth plate zones revealed mean lacunae surface areas ± SD of 100.8...
... transfer [2] Homogenous human ESC colonies may be derived from subsequent isolation and re-plating of the ICM cells, which are then screened for stemness by a variety of recognized markers Once ... substantial ethical obstacle for the advancement ofhuman ESC research With the vast therapeutic promise ofhuman ESC seen against the destruction ofhuman embryos required to realize such aspirations, ... stage, a human embryo at 2–3d post fertilization consists of just 4–8 cells, all of which are totipotent In contrast to the pluripotent cells obtained from the blastocyst ICM, one or two of these...
... NANOFIBERS FOR OSTEOGENIC DIFFERENTIATIONOFHUMAN MESENCHYMAL STEMCELLS NGUYEN THI HIEN LUONG (B.Eng., Ho Chi Minh city University of Technology, Vietnam) A THESIS SUBMITTED FOR THE DEGREE OF ... be loaded into nanofibrous scaffolds by electrospinning, and the sustained release of this drug can induce the osteogenic differentiationof MSCs e) Incorporation of Type I Col into nanofibrous ... the osteogenic differentiationof MSCs investigate the influences of prolonged culture as well as the nanofibrous structure on osteogenic differentiation function of the stemcells To produce...
... osteogenic differentiationofhuman mesenchymal stemcells Exp Cell Res (1):22-37 101 Salasznyk RM, Klees RF, Boskey A, et al 2007;Activation of FAK is necessary for the osteogenic differentiationofhuman ... differentiationof rat bone marrow -derived mesenchymal stemcells J Bone Miner Res (10):16061614 59 Matsubara T, Tsutsumi S, Pan H, et al 2004;A new technique to expand human mesenchymal stemcells using ... 2009;Mesenchymal stem cell modification of endothelial matrix regulates their vascular differentiation J Cell Biochem (4):706-713 104 Kuhn NZ, Tuan RS 2010;Regulation of stemness and stem cell niche of mesenchymal...
... Casey K Chan Effects of mechanical stimulation in osteogenic and chondrogenic differentiationof bone marrow -derived mesenchymal stemcells on nanofibrous scaffolds Journal of Bioactive and Compactable ... Types ofcells used in bone tissue engineering for osteogenic differentiation 59 2.3.1 Potential of mesenchymal stemcells (MSCs) for bone healing 60 2.3.2 Potential of bone marrow derived ... 2.3.2 Potential of bone marrow derived mesenchymal stemcells (BM-MSCs) 61 2.3.3 Potential of adipose- derivedstemcells 63 2.3.4 MSCs derived from other sources 64 2.4 Cell-material...
... CHARACTERIZATION OFHUMANADIPOSEDERIVED ADULT MULTIPOTENT PRECURSOR CELLS LEONG TAI WEI DAVID (Bachelor of Chemical Engineering, Hons) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY DEPARTMENT OF ... characterization of other potential sources ofstemcells which might avoid these issues altogether 1.1.2.1 Adult stemcellsStemcells are loosely defined as self-renewing progenitor cells that can ... that cells actually thrive in One of the promising adult stem cell sources is a culture plastic-adherent cell population from the humanadipose tissue In order to bring adiposederivedstem cells...