isolation of human adipose derived stem cells from lipoaspirates

... (2004) Isolation of mesenchymal stem cells of fetal or maternal origin from human placenta Stem Cells 22, 1338–1345 Bieback K, Kern S, Kluter H & Eichler H (2004) Critical parameters for the isolation ... differentiation of human umbilical cord blood -derived mesenchymal stem cells into hepatocytelike cells Biochem Biophys Res Commun 330, 1153–1161 Seo MJ, Suh SY, Bae YC & Jung JS (2005) Differentiation of human ... system will provide a valuable tool, in addition to primary hepatocytes, for study of the molecular basis of the regenerative and developmental processes of hepatic cells in vitro Isolation of...

Ngày tải lên: 30/03/2014, 04:20

... Immunophenotype of human adiposederived cells: temporal changes in stromal-associated and stem cell-associated markers Stem Cells 2006, 24, 376-385 26 Muir WW 3rd, Wiese AJ, March PA Effects of morphine, ... HE Stem cell therapy for neurologic disorders: therapeutic potential of adipose- derived stem cells Curr Drug Targets 2005, 6, 57-62 33 Satake K, Lou J, Lenke LG Migration of mesenchymal stem cells ... with isoflurane Am J Vet Res 2003, 64, 1155-1160 27 Ogawa R The importance of adipose- derived stem cells and vascularized tissue regeneration in the field of tissue transplantation Curr Stem Cell...

Ngày tải lên: 07/08/2014, 23:22

... good source of multipotent adult stem cells is the adipose tissue Cells isolated from these, known as ADSCs (Adipose Derived Stromal Cells) have been shown to possess the property of being multipotent ... there are two well defined types of stem cells – the embryonic stem cells and the adult stem cells The embryonic stem cells, which are found within inner cell mass of the embryonic blastocyst, is ... of stem cells and precursor cells for this purpose The two types of stem cells that are being targeted for use in tissue regeneration are the Embryonic stem cells and the Adult stem cells The...

Ngày tải lên: 08/11/2015, 17:01

... cryopreserved human adipose- derived stem cells Cryobiology 2008, 57:18-24 Chen MY, Lie PC, Li ZL, Wei X: Endothelial differentiation of Wharton’s jelly -derived mesenchymal stem cells in comparison ... endothelial cells enhance osteogenesis and vascularization of mesenchymal stem cells Cells Tissues Organs 2009, 190:185-193 Rodbell M: The metabolism of isolated fat cells IV Regulation of release of ... High-potential human mesenchymal stem cells Stem Cells Dev 2005, 14:70-80 Aust L, Devlin B, Foster SJ, Halvorsen YD, Hicok K, du Laney T, Sen A, Willingmyre GD, Gimble JM: Yield of human adipose- derived...

Ngày tải lên: 10/08/2014, 10:20

... dilutions of DCs, from 10,000 cells per well to 313 cells per well, with × 105 allogeneic CD4 T cells in a 96-well U-bottomed plate in a total volume of 200 µl for days The proliferation of T cells ... modulation of DC immunity by LV expression of different cytokines or siRNA molecules Materials and Methods Generation of monocyte -derived dendritic cells Peripheral blood mononuclear cells (PBMCs) from ... expression profile of DCs, we suspected that LV infection might also affect DC activation of T cells To test this, we set up an in vitro immunity assay using co-culture of human DCs and naïve T cells...

Ngày tải lên: 13/08/2014, 13:20

... analysis and design SL performed the stem cell isolations from benign samples PB performed stem cell isolations from tumors CH performed culturing and isolation of stem cells, and NF-κB experiments JLL ... cancer cells PTL induces apoptosis in primitive cancer cells Percent viability of prostate cancer cells and cells from a patient with BPH treated with increasing concentrations of PTL Cells were ... stellate cells highlighted the importance of culture microenvironment and the appropriate use of feeder cells in co-culture Comparison of transcriptional profiles from hepatic stellate cells cultured...

Ngày tải lên: 14/08/2014, 08:21

... 2.2 Stem cells in tissue regeneration 2.2.1 Embryonic stem cells 2.2.2 Adult stem cells 2.2.3 Adipose derived stem cells Chapter Chapter v 2.2.4 Hepatic differentiation of adipose derived stem ... of stemness marker genes on gelatin 49 microspheres 4.2.4 Adipogenic and osteogenic differentiation of 49 adipose derived stem cells 4.2.5 Hepatic differentiation of adipose derived stem 51 cells ... 3.2.2 Adipose derived stem cells isolation and culture 31 3.2.3 Cell seeding on gelatin microspheres 32 3.2.4 Total DNA quantification assay 32 3.2.5 Differetiation of adipose derived stem cells...

Ngày tải lên: 09/09/2015, 11:18

... transfer [2] Homogenous human ESC colonies may be derived from subsequent isolation and re-plating of the ICM cells, which are then screened for stemness by a variety of recognized markers Once ... stage, a human embryo at 2–3d post fertilization consists of just 4–8 cells, all of which are totipotent In contrast to the pluripotent cells obtained from the blastocyst ICM, one or two of these ... death and the creation of human embryonic stem cells J Clin Invest 2004, 114:1184-1186 Voullaire L, Slater H, Williamson R, Wilton L: Chromosome analysis of blastomeres from human embryos by using...

Ngày tải lên: 13/08/2014, 23:20

... annulus cells adiposederived mesenchymal stem of AD-MSCfor 3D co cultured alone Increase in separate culture cells (AD-MSC) and annulus cells comderived mesenchymal stem cells (AD-MSC) and annulus cells ... synthesis of proteoglycans after pellet co-culture of disc cells with BM-MSC [20] Stimulation of disc cells with stem cells or CM could enhance the success of autologous implantation of disc cells ... Chondrogenic potential of adipose tissue -derived stromal cells in vitro and in vivo Biochem Biophys Res Commun 2002, 290:763-769 Gimble JM, Guilak F: Adipose- derived adult stem cells: isolation, characterization,...

Ngày tải lên: 09/08/2014, 10:23

... approximately 40 cm3 of packed adipose tissue, fibrous tissue, red blood cells and a small number of nucleated cells An equal volume of digestive enzyme, 0.07% collagenase type 1, composed of several collagenases, ... Mesenchymal stem cell therapy in joint disease Novartis Found Symp 2003, 249:86-96 Zhang HN, Li L, Leng P, Wang YZ, Lv CY: Uninduced adipose- derived stem cells repair the defect of full-thickness ... Alfonso ZC, Fraser JK, Benhaim P, Hedrick MH: Human adipose tissue is a source of multipotent stem cells Mol Biol Cell 2002, 13:4279-4295 13 Peterkofsky B: Bacterial collagenase Methods Enzymol...

Ngày tải lên: 10/08/2014, 23:21

... tnecseroulf gniwollof ehT SBP htiw semit eerht desnir erew selpmas ehT Co73 ta h rof sllec eht htiw detabucni erew seidobitna yramirp ehT ylevitcepser ,PAFG rof 002 : dna ESN rof 002 : ta ares ... 001-X notirT %4.0 neht dna ,nim rof enoteca %001 ,nim 01 rof lonahtem %001 dloc eci htiw detaert neht erew yehT erutarepmet moor ta nim 01 rof SBP ni edyhedlamrof %7.3 htiw dexif neht dna SBP ... llec gnimrofrep dna esu lacinilc rof SBF gnisu tuohtiw oviv xe sCSM dnapxe ot sdohtem dnif ot deen ew dna ,hcraeser ruo rof SBF desu ew ,yduts tneserp ni ,revewoH noitarefilorp sCSM rof noitidnoc...

Ngày tải lên: 07/08/2014, 18:21

... that cells actually thrive in One of the promising adult stem cell sources is a culture plastic-adherent cell population from the human adipose tissue In order to bring adipose derived stem cells ... characterization of other potential sources of stem cells which might avoid these issues altogether 1.1.2.1 Adult stem cells Stem cells are loosely defined as self-renewing progenitor cells that can ... CHARACTERIZATION OF HUMAN ADIPOSE DERIVED ADULT MULTIPOTENT PRECURSOR CELLS LEONG TAI WEI DAVID (Bachelor of Chemical Engineering, Hons) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY DEPARTMENT OF...

Ngày tải lên: 12/09/2015, 09:55

... Vainzof M, Zatz M: SJL dystrophic mice express a significant amount of human muscle proteins following systemic delivery of human adipose- derived stromal cells without immunosuppression Stem Cells ... new potential source of pluripotent cells for regenerative medicine The identification of niches of tissue-specific stem cells capable of replacing damaged differentiated cells in the hFTs may ... characterization of potential sources of adult stem cells [7,10,1215], the aim of this study was to isolate, expand, characterize and assess the differentiation potential of MSCs from hFTs Methods Human...

Ngày tải lên: 18/06/2014, 15:20

... the isolation of relatively pure populations of these cells for in vitro study of the cellular processes in thalassemic stem cells While previous attempts have focused on the cell culture system, ... The isolated HSCs/CD34+ cells were harvested for phosphoproteomic analysis and cultivation of erythroid cells The cultivation of erythroid cells derived from HSCs/CD34+ cells was performed according ... culture is capable of sustaining a large number of cells, which can confer specific advantages and disadvantages The use of freshly isolated human hematopoietic stem cells from thalassemic patients...

Ngày tải lên: 18/06/2014, 19:20

... the isolation of relatively pure populations of these cells for in vitro study of the cellular processes in thalassemic stem cells While previous attempts have focused on the cell culture system, ... The isolated HSCs/CD34+ cells were harvested for phosphoproteomic analysis and cultivation of erythroid cells The cultivation of erythroid cells derived from HSCs/CD34+ cells was performed according ... culture is capable of sustaining a large number of cells, which can confer specific advantages and disadvantages The use of freshly isolated human hematopoietic stem cells from thalassemic patients...

Ngày tải lên: 20/06/2014, 04:20

... extract fatty acid from the bacterial cell-wall and identified with microbial identification system (MIDI, Inc., USA) Isolation of bacteria: One hundred strains of LAB were isolated from selective ... without bacterial culture Isolation of cholesterol-lowering lactic acid bacteria 393 Table Number of acid- and bile-tolerant lactic acid bacteria isolated from the human intestine for probiotic ... (sodium salt of taurodeoxycholic acid) and 0.37% g/l CaCl2; B, MRS as control; Bile-salt hydrolysis positive was represented by black arrow Fig Schematic diagrams for the isolation of human intestinal...

Ngày tải lên: 07/08/2014, 18:20

... access, cells from the IPFP are of interest as a potential source of cells for the repair of focal cartilage defects in the knee [5] In previous work we demonstrated the ability of IPFPderived cells ... of GAG per cell These results with IPFP cells are comparable to those from a previous study on stem cells derived from other human liposuction -derived adipose tissue, in which 5% oxygen was reported ... reported in 2% oxygen [36] The effects of hypoxia on the propagation of human bone marrow -derived stem cells suggested that 2% oxygen favoured more primitive stem cells with higher colony-forming unit...

Ngày tải lên: 09/08/2014, 10:20

... staining of IPFPderived cells It was therefore interesting that they showed a pattern of expression on a high proportion of the IPFP -derived cells and of epitopes commonly abundant on adult stem cells ... characterisation of infrapatellar fat padderived cells The rate of proliferation of the IPFP -derived cells in monolayer culture was significantly increased by FGF-2 A comparison of their proliferation ... tissues [19], and indeed many of the tissues from which stem cells have been isolated have good vascularisation A minor population of bone marrow -derived mesenchymal stem cells has also been found...

Ngày tải lên: 09/08/2014, 10:23

... induced cells (A) Cells in G0–G1 phases were gated under R1 and cells blocked in M phase under R2 Propidium iodide fluorescence of control cells is shown by filled bars, paclitaxel induced cells ... carcinoma tissue sections and in HeLa cells Total protein of fresh milk obtained from human volunteers was extracted between five and eight times with chloroform/ methanol (1 : and : 1, v/v) at ... proteinÆmL)1 For isolation of MLGM-associated proteins, MLGM fractions were separated from milk by sucrose gradient centrifugation, and then proteins were further separated from lipids by chloroform/methanol...

Ngày tải lên: 23/03/2014, 21:20

... http://www.translational-medicine.com/content/7/1/6 Materials and methods Isolation and characterization of human CD34+ cells from the umbilical cord blood CD34+ stem cells from human umbilical cord were obtained from healthy babies, born ... after transdifferentiation of adherent cells obtained from prior CD34+ stem cells Nc) normal control (human skeletal muscle) SC) CD34+ stem cells aSC) adherent stem cells (prior CD34+) Emery AEH: ... differentiation of mesenchymal stem cells isolated from human umbilical cord blood Stem Cells 2004, 22(4):617-624 Nunes VA, Cavaçana N, Canovas M, Strauss BE, Zatz M: Stem cells from umbilical...

Ngày tải lên: 18/06/2014, 15:20