... (2004) Isolationof mesenchymal stemcellsof fetal or maternal origin fromhuman placenta StemCells 22, 1338–1345 Bieback K, Kern S, Kluter H & Eichler H (2004) Critical parameters for the isolation ... differentiation ofhuman umbilical cord blood -derived mesenchymal stemcells into hepatocytelike cells Biochem Biophys Res Commun 330, 1153–1161 Seo MJ, Suh SY, Bae YC & Jung JS (2005) Differentiation ofhuman ... system will provide a valuable tool, in addition to primary hepatocytes, for study of the molecular basis of the regenerative and developmental processes of hepatic cells in vitro Isolation of...
... Immunophenotype ofhuman adiposederived cells: temporal changes in stromal-associated and stem cell-associated markers StemCells 2006, 24, 376-385 26 Muir WW 3rd, Wiese AJ, March PA Effects of morphine, ... HE Stem cell therapy for neurologic disorders: therapeutic potential of adipose- derivedstemcells Curr Drug Targets 2005, 6, 57-62 33 Satake K, Lou J, Lenke LG Migration of mesenchymal stemcells ... with isoflurane Am J Vet Res 2003, 64, 1155-1160 27 Ogawa R The importance of adipose- derivedstemcells and vascularized tissue regeneration in the field of tissue transplantation Curr Stem Cell...
... good source of multipotent adult stemcells is the adipose tissue Cells isolated from these, known as ADSCs (Adipose Derived Stromal Cells) have been shown to possess the property of being multipotent ... there are two well defined types ofstemcells – the embryonic stemcells and the adult stemcells The embryonic stem cells, which are found within inner cell mass of the embryonic blastocyst, is ... ofstemcells and precursor cells for this purpose The two types ofstemcells that are being targeted for use in tissue regeneration are the Embryonic stemcells and the Adult stemcells The...
... cryopreserved human adipose- derivedstemcells Cryobiology 2008, 57:18-24 Chen MY, Lie PC, Li ZL, Wei X: Endothelial differentiation of Wharton’s jelly -derived mesenchymal stemcells in comparison ... endothelial cells enhance osteogenesis and vascularization of mesenchymal stemcellsCells Tissues Organs 2009, 190:185-193 Rodbell M: The metabolism of isolated fat cells IV Regulation of release of ... High-potential human mesenchymal stemcellsStemCells Dev 2005, 14:70-80 Aust L, Devlin B, Foster SJ, Halvorsen YD, Hicok K, du Laney T, Sen A, Willingmyre GD, Gimble JM: Yield ofhuman adipose- derived...
... dilutions of DCs, from 10,000 cells per well to 313 cells per well, with × 105 allogeneic CD4 T cells in a 96-well U-bottomed plate in a total volume of 200 µl for days The proliferation of T cells ... modulation of DC immunity by LV expression of different cytokines or siRNA molecules Materials and Methods Generation of monocyte -derived dendritic cells Peripheral blood mononuclear cells (PBMCs) from ... expression profile of DCs, we suspected that LV infection might also affect DC activation of T cells To test this, we set up an in vitro immunity assay using co-culture ofhuman DCs and naïve T cells...
... analysis and design SL performed the stem cell isolations from benign samples PB performed stem cell isolations from tumors CH performed culturing and isolationofstem cells, and NF-κB experiments JLL ... cancer cells PTL induces apoptosis in primitive cancer cells Percent viability of prostate cancer cells and cellsfrom a patient with BPH treated with increasing concentrations of PTL Cells were ... stellate cells highlighted the importance of culture microenvironment and the appropriate use of feeder cells in co-culture Comparison of transcriptional profiles from hepatic stellate cells cultured...
... transfer [2] Homogenous human ESC colonies may be derivedfrom subsequent isolation and re-plating of the ICM cells, which are then screened for stemness by a variety of recognized markers Once ... stage, a human embryo at 2–3d post fertilization consists of just 4–8 cells, all of which are totipotent In contrast to the pluripotent cells obtained from the blastocyst ICM, one or two of these ... death and the creation ofhuman embryonic stemcells J Clin Invest 2004, 114:1184-1186 Voullaire L, Slater H, Williamson R, Wilton L: Chromosome analysis of blastomeres fromhuman embryos by using...
... annulus cells adiposederived mesenchymal stemof AD-MSCfor 3D co cultured alone Increase in separate culture cells (AD-MSC) and annulus cells comderived mesenchymal stemcells (AD-MSC) and annulus cells ... synthesis of proteoglycans after pellet co-culture of disc cells with BM-MSC [20] Stimulation of disc cells with stemcells or CM could enhance the success of autologous implantation of disc cells ... Chondrogenic potential ofadipose tissue -derived stromal cells in vitro and in vivo Biochem Biophys Res Commun 2002, 290:763-769 Gimble JM, Guilak F: Adipose- derived adult stem cells: isolation, characterization,...
... approximately 40 cm3 of packed adipose tissue, fibrous tissue, red blood cells and a small number of nucleated cells An equal volume of digestive enzyme, 0.07% collagenase type 1, composed of several collagenases, ... Mesenchymal stem cell therapy in joint disease Novartis Found Symp 2003, 249:86-96 Zhang HN, Li L, Leng P, Wang YZ, Lv CY: Uninduced adipose- derivedstemcells repair the defect of full-thickness ... Alfonso ZC, Fraser JK, Benhaim P, Hedrick MH: Humanadipose tissue is a source of multipotent stemcells Mol Biol Cell 2002, 13:4279-4295 13 Peterkofsky B: Bacterial collagenase Methods Enzymol...
... that cells actually thrive in One of the promising adult stem cell sources is a culture plastic-adherent cell population from the humanadipose tissue In order to bring adiposederivedstemcells ... characterization of other potential sources ofstemcells which might avoid these issues altogether 1.1.2.1 Adult stemcellsStemcells are loosely defined as self-renewing progenitor cells that can ... CHARACTERIZATION OFHUMANADIPOSEDERIVED ADULT MULTIPOTENT PRECURSOR CELLS LEONG TAI WEI DAVID (Bachelor of Chemical Engineering, Hons) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY DEPARTMENT OF...
... Vainzof M, Zatz M: SJL dystrophic mice express a significant amount ofhuman muscle proteins following systemic delivery ofhuman adipose- derived stromal cells without immunosuppression StemCells ... new potential source of pluripotent cells for regenerative medicine The identification of niches of tissue-specific stemcells capable of replacing damaged differentiated cells in the hFTs may ... characterization of potential sources of adult stemcells [7,10,1215], the aim of this study was to isolate, expand, characterize and assess the differentiation potential of MSCs from hFTs Methods Human...
... the isolationof relatively pure populations of these cells for in vitro study of the cellular processes in thalassemic stemcells While previous attempts have focused on the cell culture system, ... The isolated HSCs/CD34+ cells were harvested for phosphoproteomic analysis and cultivation of erythroid cells The cultivation of erythroid cellsderivedfrom HSCs/CD34+ cells was performed according ... culture is capable of sustaining a large number of cells, which can confer specific advantages and disadvantages The use of freshly isolated human hematopoietic stemcellsfrom thalassemic patients...
... the isolationof relatively pure populations of these cells for in vitro study of the cellular processes in thalassemic stemcells While previous attempts have focused on the cell culture system, ... The isolated HSCs/CD34+ cells were harvested for phosphoproteomic analysis and cultivation of erythroid cells The cultivation of erythroid cellsderivedfrom HSCs/CD34+ cells was performed according ... culture is capable of sustaining a large number of cells, which can confer specific advantages and disadvantages The use of freshly isolated human hematopoietic stemcellsfrom thalassemic patients...
... extract fatty acid from the bacterial cell-wall and identified with microbial identification system (MIDI, Inc., USA) Isolationof bacteria: One hundred strains of LAB were isolated from selective ... without bacterial culture Isolationof cholesterol-lowering lactic acid bacteria 393 Table Number of acid- and bile-tolerant lactic acid bacteria isolated from the human intestine for probiotic ... (sodium salt of taurodeoxycholic acid) and 0.37% g/l CaCl2; B, MRS as control; Bile-salt hydrolysis positive was represented by black arrow Fig Schematic diagrams for the isolationofhuman intestinal...
... access, cellsfrom the IPFP are of interest as a potential source ofcells for the repair of focal cartilage defects in the knee [5] In previous work we demonstrated the ability of IPFPderived cells ... of GAG per cell These results with IPFP cells are comparable to those from a previous study on stemcellsderivedfrom other human liposuction -derived adipose tissue, in which 5% oxygen was reported ... reported in 2% oxygen [36] The effects of hypoxia on the propagation ofhuman bone marrow -derived stemcells suggested that 2% oxygen favoured more primitive stemcells with higher colony-forming unit...
... staining of IPFPderived cells It was therefore interesting that they showed a pattern of expression on a high proportion of the IPFP -derived cells and of epitopes commonly abundant on adult stemcells ... characterisation of infrapatellar fat padderived cells The rate of proliferation of the IPFP -derived cells in monolayer culture was significantly increased by FGF-2 A comparison of their proliferation ... tissues [19], and indeed many of the tissues from which stemcells have been isolated have good vascularisation A minor population of bone marrow -derived mesenchymal stemcells has also been found...
... induced cells (A) Cells in G0–G1 phases were gated under R1 and cells blocked in M phase under R2 Propidium iodide fluorescence of control cells is shown by filled bars, paclitaxel induced cells ... carcinoma tissue sections and in HeLa cells Total protein of fresh milk obtained fromhuman volunteers was extracted between five and eight times with chloroform/ methanol (1 : and : 1, v/v) at ... proteinÆmL)1 For isolationof MLGM-associated proteins, MLGM fractions were separated from milk by sucrose gradient centrifugation, and then proteins were further separated from lipids by chloroform/methanol...
... http://www.translational-medicine.com/content/7/1/6 Materials and methods Isolation and characterization ofhuman CD34+ cellsfrom the umbilical cord blood CD34+ stemcellsfromhuman umbilical cord were obtained from healthy babies, born ... after transdifferentiation of adherent cells obtained from prior CD34+ stemcells Nc) normal control (human skeletal muscle) SC) CD34+ stemcells aSC) adherent stemcells (prior CD34+) Emery AEH: ... differentiation of mesenchymal stemcells isolated fromhuman umbilical cord blood StemCells 2004, 22(4):617-624 Nunes VA, Cavaçana N, Canovas M, Strauss BE, Zatz M: Stemcellsfrom umbilical...