current animal applications of adipose derived stem cells

... HE Stem cell therapy for neurologic disorders: therapeutic potential of adipose -derived stem cells Curr Drug Targets 2005, 6, 57-62 33 Satake K, Lou J, Lenke LG Migration of mesenchymal stem cells ... G, Wu X, Gimble JM Immunophenotype of human adiposederived cells: temporal changes in stromal-associated and stem cell-associated markers Stem Cells 2006, 24, 376-385 26 Muir WW 3rd, Wiese AJ, ... YH Soluble factors-mediated immunomodulatory effects of canine adipose tissue -derived mesenchymal stem cells Stem Cells Dev 2008, 17, 681-693 Kang SK, Lee DH, Bae YC, Kim HK, Baik SY, Jung JS...

Ngày tải lên: 07/08/2014, 23:22

... [23] Besides, although bone marrowderived MSC is the major source of stem cells in these studies [22,24-27], the therapeutic role of adiposederived mesenchymal stem cells (ADMSCs) in acute IR injury ... Laboratory Animals (NIH publication No Page of 13 85-23, National Academy Press, Washington, DC, USA, revised 1996) Animal Grouping and Isolation of Adipose -Derived Mesenchymal Stem Cells Pathogen-free, ... endotoxin-induced systemic response by bone marrow -derived mesenchymal stem cells in mice Am J Physiol Lung Cell Mol Physiol 2007, 293:L131-141 23 Salgar S, Manning E, Ruiz P, Pham S: Mesenchymal Stem Cell...

Ngày tải lên: 18/06/2014, 22:20

... 367(Suppl):S244-S253 Barry FP: Mesenchymal stem cell therapy in joint disease Novartis Found Symp 2003, 249:86-96 Zhang HN, Li L, Leng P, Wang YZ, Lv CY: Uninduced adipose -derived stem cells repair the defect of ... autologous mesenchymal stem cells Pain Physician 2008, 11:343-353 Lee JS, Lee JS, Roh HL, Kim CH, Jung JS, Suh KT: Alterations in the differentiation ability of mesenchymal stem cells in patients ... multipotent stem cells Mol Biol Cell 2002, 13:4279-4295 13 Peterkofsky B: Bacterial collagenase Methods Enzymol 1982, 82:453-471 14 Salem H, Thiemermann C: Mesenchymal stromal cells: current understanding...

Ngày tải lên: 10/08/2014, 23:21

... mesenchymal stem cells of fetal or maternal origin from human placenta Stem Cells 22, 1338–1345 Bieback K, Kern S, Kluter H & Eichler H (2004) Critical parameters for the isolation of mesenchymal stem cells ... tissue -derived mesenchymal stem cells (AT-MSCs) differentiate into hepatocyte-like cells upon treatment with hepatocyte growth factor (HGF), oncostatin M and dimethyl sulfoxide [13] These cells ... blood -derived mesenchymal stem cells into hepatocytelike cells Biochem Biophys Res Commun 330, 1153–1161 Seo MJ, Suh SY, Bae YC & Jung JS (2005) Differentiation of human adipose stromal cells...

Ngày tải lên: 30/03/2014, 04:20

... cardiac damage Presently there are two well defined types of stem cells – the embryonic stem cells and the adult stem cells The embryonic stem cells, which are found within inner cell mass of the embryonic ... is currently being focused on the use of stem cells and precursor cells for this purpose The two types of stem cells that are being targeted for use in tissue regeneration are the Embryonic stem ... adult stem cells is the adipose tissue Cells isolated from these, known as ADSCs (Adipose Derived Stromal Cells) have been shown to possess the property of being multipotent and can give rise to cells...

Ngày tải lên: 08/11/2015, 17:01

... efficient and safe xeno-free cryopreservation method for the storage of human embryonic stem cells Stem Cells 2004;22(5):779-89 10 Speicher MR, Ballard SG, Ward DC Karyotyping human chromosomes ... Marshall VS, Jones JM Embryonic stem cell lines derived from human blastocysts Science 1998;282(5391):1145-7 Reubinoff BE, Pera MF, Fong CY, Trounson A, Bongso A Embryonic stem cell lines from human ... some cells detached after exposure to low temperature Virtually all of the detached cells were determined to be non-viable with tryphan blue staining (data not shown) All of the detached cells...

Ngày tải lên: 31/10/2012, 16:57

... ES cells and in human hematopoietic progenitor cells Discussion B Fig Transcription of LCR hypersensitive site in undifferentiated murine embryonic stem cells and in human CD 133+ bone marrow cells ... Rex-1 gene, primer sets span introns Day cells are composed of ES cells and MEF cells grown in ES media containing leukemia inhibitory factor (LIF) These cells express the Rex-1 and b-actin genes ... differentiation of murine embryonic stem cells PCR analysis of DNase I treated and reverse-transcribed total RNA extracted from differentiating embryonic stem cells at the indicated time points...

Ngày tải lên: 07/03/2014, 12:20

... pluripotent stem cells (Takahashi and Yamanaka, 2006) To realize the full potential of embryonic and induced pluripotent stem cells, technologies, and especially those related to stem cell-based ... Chapter Methods for the Derivation of Human Embryonic Stem Cell Lines Abstract Human embryonic stem cells (hESCs) are pluripotent cells derived from the inner cell mass (ICM) of the developing ... Stice S (2003) Human embryonic stem cell lines derived from discarded embryos Stem Cells 21:521–526 Reubinoff BE, Pera MF, Fong C, Trounson A, Bongso A (2000) Embryonic stem cell lines from human...

Ngày tải lên: 22/03/2014, 09:20

... human [16,17] and mouse embryonic stem cells [18-20] The unique patterns of miRNA expression in embryonic stem cells suggest they are involved in maintaining "stemness" Identifying mRNAs that ... miRNA upregulated in hES cells, 31 upregulated in EB cells, and 35 upregulated in adult cells (Figure 2) The 20 miRNAs most highly expressed in hES cells, EB, and adult cells respectively were ... supervised clustering (A) Adult cells and EB shared genes (B) hES cells upregulated genes (C) EB upregulated genes (D) Adult cells upregulated genes hESC cells EB adult cells Figure supervised hierarchical...

Ngày tải lên: 18/06/2014, 15:20

... activity of patient -derived PBMNC derived from L1 to L6 and L7 to L9 against K562 cells (A) and autologous In vitro (B) In vitro cytolytic activity of patient -derived PBMNC derived from L1 to ... lymphocyte subpopulations such as CD3+, CD3+/CD4+and CD3+/CD8+ T cells, CD19+ B cells, CD3+/CD16/56+ NKlike T cells, and CD3-/CD16/56+ NK cells in the peripheral blood were within normal levels throughout ... nuclear cells, lymphocytes, NK cells in the leukapheresis products 1.1 see 1.5 Total nuclear cells (×1010) 7.6 8.5 Total lymphocytes (×109) Lymphocytes (%) (69) (57) 1.8 1.3 Total NK cells (×109)...

Ngày tải lên: 18/06/2014, 15:20

... erythroid cells The cultivation of erythroid cells derived from HSCs/CD34+ cells was performed according to a procedure previously described [13] with some modifications HSCs/CD34 + cells were ... relatively pure populations of these cells for in vitro study of the cellular processes in thalassemic stem cells While previous attempts have focused on the cell culture system, in this study the phosphoproteome ... of apoptosis and the relevant signaling pathways in hematopoietic stem cells are still poorly understood Hematopoietic stem cells (HSCs) contain specific markers on their surface such as CD34...

Ngày tải lên: 18/06/2014, 19:20

... trabecular bone area); fat cells diameter = the total diameter of fat cells in selected field/the number of fat cells in selected field; fat cells area = the area of all fat cells in selected field/(the ... mean fat cells size and fat cells area would be measured with imaging process software Image-Pro Plus 5.1 (Media Cybernetics Inc., Silver Spring, MD) Fat cells density = marrow fat cells number ... of marrow mesenchymal stem cells (MSCs) [7] Results of a previous study showed increased number of small size fat cells in the early steroid-associated ON, that might be derived from the adipogenic...

Ngày tải lên: 20/06/2014, 00:20

... erythroid cells The cultivation of erythroid cells derived from HSCs/CD34+ cells was performed according to a procedure previously described [13] with some modifications HSCs/CD34 + cells were ... relatively pure populations of these cells for in vitro study of the cellular processes in thalassemic stem cells While previous attempts have focused on the cell culture system, in this study the phosphoproteome ... of apoptosis and the relevant signaling pathways in hematopoietic stem cells are still poorly understood Hematopoietic stem cells (HSCs) contain specific markers on their surface such as CD34...

Ngày tải lên: 20/06/2014, 04:20

... endogenous neural progenitor or stem cells and the transplantation of adult -derived neural progenitor and stem cells [39] Self-renewing multipotent neural progenitor and stem cells have been isolated ... the nervous system Adult -derived neural progenitor and stem cells grafted in the brain promote neuroprotection, by an immunomodulatory mechanism Grafted neural progenitor and stem cells interact ... brain originate from residual stem cells The existence of stem cells in the adult brain suggests that it has the potential for self-repair and that newborn neuronal cells may contribute to the...

Ngày tải lên: 08/08/2014, 17:20

... Myogenesis Most investigations of myogenesis in adult stem cells are based on a small population of skeletal muscle -derived stem cells, or satellite cells A recent study showed the highly successful ... techniques can be used to label stem cells [79] In other systems, asymmetric division has been shown to be integral to stem cell self-renewal This unique property of stem cells has been exploited to ... for self-renewal of human multipotent adipose -derived stem cells Stem Cells 2006, 24:2412-2419 Kleber M, Sommer L: Wnt signaling and the regulation of stem cell function Curr Opin Cell Biol 2004,...

Ngày tải lên: 09/08/2014, 10:20

... of cells was determined spectrophotometrically entiation of human mesenchymal transcripts Expression of BMP-2 and chordinstem cells during osteogenic differentiation of human mesenchymal stem cells ... assay at 3, and days of culture After and days, cells treated with chordin siRNA had decreased proliferation, as compared with untreated cells, and cells treated with transfection reagent only or ... factor and bone morphogenetic protein-2 on osteogenic differentiation of rat bone marrow -derived mesenchymal stem cells J Bone Miner Res 1997, 12:1606-1614 Ogasawara T, Kawaguchi H, Jinno S, Hoshi...

Ngày tải lên: 09/08/2014, 10:23

... IPFPderived cells It was therefore interesting that they showed a pattern of expression on a high proportion of the IPFP -derived cells and of epitopes commonly abundant on adult stem cells derived ... was derived from haematopoetic, neural, or myogenic progenitors or stem cells Evidence for pericytes in the IPFP tissue and IPFPderived cells 3G5 distinctively stains pericytes and these cells ... marrow -derived mesenchymal stem cells, and we have determined a baseline for the use of FGF-2 in the culture of fat pad -derived cells Conclusion The present study showed that IPFP tissue contained cells...

Ngày tải lên: 09/08/2014, 10:23

... use of bone marrow -derived MSCs and other effects may be seen when different cells are employed Orthotopic BMP-4 gene delivery via retrovirus transduction of muscle -derived stem cells was shown ... label viable cells This combination allows the differentiation among early apoptotic cells (Ann5 positive, 6-CFDA positive), necrotic cells (Ann5 positive, 6-CFDA negative), and viable cells (Ann5 ... mesenchymal stem cells for osteoarthritis therapy Nat Clin Pract Rheumatol 2008, 4:371-380 Estes BT, Wu AW, Guilak F: Potent induction of chondrocytic differentiation of human adipose -derived adult stem...

Ngày tải lên: 09/08/2014, 14:22

... size-sieved stem cells into electrically active neural cells Stem Cells 2002, 20(6):522-529 Tzeng SF, Tsai MJ, Hung SC, Cheng H: Neuronal morphological change of size-sieved stem cells induced ... mesenchymal stem cells into insulin producing cells J Biomed Mater Res A 2008, 86(4):1097-1105 Chao KC, Chao KF, Fu YS, Liu SH: Islet-like clusters derived from mesenchymal stem cells in Wharton’s ... cells on supporting endogenous cells to regenerate However, the potential of bone marrow stem cells or MSCs to differentiate into IPCs has been demonstrated in vitro and in vivo Bone marrow cells...

Ngày tải lên: 10/08/2014, 05:21