orthopedic use of adipose derived stem cells

... HE Stem cell therapy for neurologic disorders: therapeutic potential of adipose- derived stem cells Curr Drug Targets 2005, 6, 57-62 33 Satake K, Lou J, Lenke LG Migration of mesenchymal stem cells ... Immunophenotype of human adiposederived cells: temporal changes in stromal-associated and stem cell-associated markers Stem Cells 2006, 24, 376-385 26 Muir WW 3rd, Wiese AJ, March PA Effects of morphine, ... with isoflurane Am J Vet Res 2003, 64, 1155-1160 27 Ogawa R The importance of adipose- derived stem cells and vascularized tissue regeneration in the field of tissue transplantation Curr Stem Cell...

Ngày tải lên: 07/08/2014, 23:22

... and Use of Laboratory Animals (NIH publication No Page of 13 85-23, National Academy Press, Washington, DC, USA, revised 1996) Animal Grouping and Isolation of Adipose- Derived Mesenchymal Stem Cells ... therapeutic role of adiposederived mesenchymal stem cells (ADMSCs) in acute IR injury of the lung has not been investigated Recently, ADMSCs have been reported to have the distinct advantages of being ... parenchyma from IR damage Figure Impact of Adipose- Derived Mesenchymal Stem Cells (ADMSC) Transplantation on the Severity of IR-Induced Lung Parenchymal Injury Number of alveolar sacs and crowded area...

Ngày tải lên: 18/06/2014, 22:20

... approximately 40 cm3 of packed adipose tissue, fibrous tissue, red blood cells and a small number of nucleated cells An equal volume of digestive enzyme, 0.07% collagenase type 1, composed of several collagenases, ... made active use of her bilateral knee joints With a diagnosis of osteoarthritis of Pak Journal of Medical Case Reports 2011, 5:296 http://www.jmedicalcasereports.com/content/5/1/296 Page of Table ... Mesenchymal stem cell therapy in joint disease Novartis Found Symp 2003, 249:86-96 Zhang HN, Li L, Leng P, Wang YZ, Lv CY: Uninduced adipose- derived stem cells repair the defect of full-thickness...

Ngày tải lên: 10/08/2014, 23:21

... mesenchymal stem cells of fetal or maternal origin from human placenta Stem Cells 22, 1338–1345 Bieback K, Kern S, Kluter H & Eichler H (2004) Critical parameters for the isolation of mesenchymal stem cells ... evidence of transdifferentiation by MET in the process of hepatic differentiation of AT-MSCs No previous report has demonstrated evidence of transdifferentiation of committed adult stem cells In ... development of in vitro test systems Although primary human hepatocytes are the best source of cells for such systems, their use for this purpose is limited Transcriptome in hepatic induction of AT-MSCs...

Ngày tải lên: 30/03/2014, 04:20

... being focused on the use of stem cells and precursor cells for this purpose The two types of stem cells that are being targeted for use in tissue regeneration are the Embryonic stem cells and ... there are two well defined types of stem cells – the embryonic stem cells and the adult stem cells The embryonic stem cells, which are found within inner cell mass of the embryonic blastocyst, is ... good source of multipotent adult stem cells is the adipose tissue Cells isolated from these, known as ADSCs (Adipose Derived Stromal Cells) have been shown to possess the property of being multipotent...

Ngày tải lên: 08/11/2015, 17:01

... adsorption of the antibodies After another wash in PBS (3×), the cells were incubated with a mixture of diluted primary antibodies against SSEA-3 (mouse IgM, 10 μg/ml) and TRA-1-81 (mouse IgG, ... and m-FISH (Figure 3) of metaphase spreads Hence, the results of this study could have useful implications for the salvage of hESC culture during infrequent occurrences of incubator break-down ... bioreactors Conflicts of interest The authors have declared that no conflict of interest exists References Becker EW The roles of ATP in the dynamics of the actin filaments of the cytoskeleton...

Ngày tải lên: 31/10/2012, 16:57

... cases except that of the Rex-1 gene, primer sets span introns Day cells are composed of ES cells and MEF cells grown in ES media containing leukemia inhibitory factor (LIF) These cells express the ... the differentiation of hematopoietic stem cells to erythroid cells Transcription of HS3 could be important in maintaining this accessible structure, particularly in light of the fact that RNA ... differentiation of mouse embryonic stem cells to hematopoietic cells on an OP9 stromal cell monolayer Methods Enzymol 365, 72–83 21 Strahl BD, Ohba R, Cook RG & Allis CD (1999) Methylation of histone...

Ngày tải lên: 07/03/2014, 12:20

... expansion of human embryonic stem cells, and more recently, of human induced pluripotent stem cells The exploration and realization of the incredible potential of these pluripotent stem cells for ... the field of stem cells It is also our hope that the methods, descriptions and images provided in this atlas facilitate the realization of the enormous potential of human pluripotent stem cells and ... Methods for the Derivation of Human Embryonic Stem Cell Lines Abstract Human embryonic stem cells (hESCs) are pluripotent cells derived from the inner cell mass (ICM) of the developing embryo They...

Ngày tải lên: 22/03/2014, 09:20

... reported in human [16,17] and mouse embryonic stem cells [18-20] The unique patterns of miRNA expression in embryonic stem cells suggest they are involved in maintaining "stemness" Identifying mRNAs ... 13 The expression of miR-92 has been reported in human embryonic stem (ES) cells [16,26], mouse ES cells[ 20] or Page 12 of 17 (page number not for citation purposes) Journal of Translational Medicine ... hES cells and adult cells was also significant (P < 0.05) In particular, the expression of miR-519b was 8-fold greater in hES cells than in EB cells and it was not even detected in adult cells...

Ngày tải lên: 18/06/2014, 15:20

... The presence of CD3+ and CD45+ cells was used as an indicator of T cell infiltration and the prevalence of CD4+, CD8+, CD56+ cells as indicators of T helper, T cytotoxic and NK cells, respectively ... cytolytic activity of patient -derived PBMNC derived from L1 to L6 and L7 to L9 against K562 cells (A) and autologous In vitro (B) In vitro cytolytic activity of patient -derived PBMNC derived from L1 ... A- Photomicrograph view of the patient -derived cell line of the anastomotic relapse A- Photomicrograph view of the patient -derived cell line of the anastomotic relapse Cells were cultured and...

Ngày tải lên: 18/06/2014, 15:20

... isolation of relatively pure populations of these cells for in vitro study of the cellular processes in thalassemic stem cells While previous attempts have focused on the cell culture system, in ... erythroid cells The cultivation of erythroid cells derived from HSCs/CD34+ cells was performed according to a procedure previously described [13] with some modifications HSCs/CD34 + cells were ... hematopoietic stem cells for studying cell signaling in the apoptotic pathway because of the limited amount of sample from patients and normal donors On the other hand, in vitro culture is capable of sustaining...

Ngày tải lên: 18/06/2014, 19:20

... of the adipogenesis of marrow mesenchymal stem cells (MSCs) [7] Results of a previous study showed increased number of small size fat cells in the early steroid-associated ON, that might be derived ... trabecular bone area); fat cells diameter = the total diameter of fat cells in selected field/the number of fat cells in selected field; fat cells area = the area of all fat cells in selected field/(the ... mean fat cells size and fat cells area would be measured with imaging process software Image-Pro Plus 5.1 (Media Cybernetics Inc., Silver Spring, MD) Fat cells density = marrow fat cells number...

Ngày tải lên: 20/06/2014, 00:20

... isolation of relatively pure populations of these cells for in vitro study of the cellular processes in thalassemic stem cells While previous attempts have focused on the cell culture system, in ... erythroid cells The cultivation of erythroid cells derived from HSCs/CD34+ cells was performed according to a procedure previously described [13] with some modifications HSCs/CD34 + cells were ... hematopoietic stem cells for studying cell signaling in the apoptotic pathway because of the limited amount of sample from patients and normal donors On the other hand, in vitro culture is capable of sustaining...

Ngày tải lên: 20/06/2014, 04:20

... stimulation of endogenous neural progenitor or stem cells and the transplantation of adult -derived neural progenitor and stem cells [39] Self-renewing multipotent neural progenitor and stem cells have ... brain originate from residual stem cells The existence of stem cells in the adult brain suggests that it has the potential for self-repair and that newborn neuronal cells may contribute to the ... progenitor or stem cells locally would represent a strategy to promote regeneration of the diseased and injured nervous system Alternatively, new neuronal cells are generated at sites of degeneration...

Ngày tải lên: 08/08/2014, 17:20

... the induction of MSC adipogenesis Myogenesis Most investigations of myogenesis in adult stem cells are based on a small population of skeletal muscle -derived stem cells, or satellite cells A recent ... critical for self-renewal of human multipotent adipose- derived stem cells Stem Cells 2006, 24:2412-2419 Kleber M, Sommer L: Wnt signaling and the regulation of stem cell function Curr Opin Cell Biol ... mesenchymal stem cells Stem Cells 2004, 22:849-860 Gronthos S, Franklin DM, Leddy HA, Robey PG, Storms RW, Gimble JM: Surface protein characterization of human adipose tissue -derived stromal cells...

Ngày tải lên: 09/08/2014, 10:20

... number of cells was determined spectrophotometrically entiation of human mesenchymal transcripts Expression of BMP-2 and chordinstem cells during osteogenic differentiation of human mesenchymal stem ... extrapolated to cells from a large population of donors because there is consistently a significant and large increase in expression of osteogenic markers, as a result of chordin knockdown in cells from ... suppression of chordin led to an increase in the bioavailability of endogenously produced BMP-2 to drive the differentiation of Page of (page number not for citation purposes) Effect of chordin...

Ngày tải lên: 09/08/2014, 10:23

... staining of IPFPderived cells It was therefore interesting that they showed a pattern of expression on a high proportion of the IPFP -derived cells and of epitopes commonly abundant on adult stem cells ... FGF2 is routinely used in the culture of bone marrow -derived mesenchymal stem cells, and we have determined a baseline for the use of FGF-2 in the culture of fat pad -derived cells Conclusion The ... Clonal IPFP -derived cells retained the cell surface characteristics of the parent IPFP cells, which were similar to mesenchymal stem cells The clonal populations of IPFP -derived cells appeared...

Ngày tải lên: 09/08/2014, 10:23

... mesenchymal stem cells for osteoarthritis therapy Nat Clin Pract Rheumatol 2008, 4:371-380 Estes BT, Wu AW, Guilak F: Potent induction of chondrocytic differentiation of human adipose- derived adult stem ... members of the TGF-β superfamily reveal considerable hypertrophy and high levels of COL X expression Although the use of COL X as a marker of chondrogenic hypertrophy in MSC-based systems has ... arcades of Benninghoff [29] and by the appearance of hypertrophic cells Analyses of lacunae surface areas in the different growth plate zones revealed mean lacunae surface areas ± SD of 100.8...

Ngày tải lên: 09/08/2014, 14:22

... differentiation of size-sieved stem cells into electrically active neural cells Stem Cells 2002, 20(6):522-529 Tzeng SF, Tsai MJ, Hung SC, Cheng H: Neuronal morphological change of size-sieved stem cells ... and survival of adherent cells [17] FN and LAM constitute the major ECM of pancreas islet cells Laminin-1 has been reported to promote the differentiation of fetal mouse pancreatic b -cells [18] ... differentiation of human mesenchymal stem cells into insulin producing cells J Biomed Mater Res A 2008, 86(4):1097-1105 Chao KC, Chao KF, Fu YS, Liu SH: Islet-like clusters derived from mesenchymal stem cells...

Ngày tải lên: 10/08/2014, 05:21