current human applications of adipose derived stem cells

... Immunophenotype of human adiposederived cells: temporal changes in stromal-associated and stem cell-associated markers Stem Cells 2006, 24, 376-385 26 Muir WW 3rd, Wiese AJ, March PA Effects of morphine, ... HE Stem cell therapy for neurologic disorders: therapeutic potential of adipose- derived stem cells Curr Drug Targets 2005, 6, 57-62 33 Satake K, Lou J, Lenke LG Migration of mesenchymal stem cells ... with isoflurane Am J Vet Res 2003, 64, 1155-1160 27 Ogawa R The importance of adipose- derived stem cells and vascularized tissue regeneration in the field of tissue transplantation Curr Stem Cell...

Ngày tải lên: 07/08/2014, 23:22

... staining of IPFPderived cells It was therefore interesting that they showed a pattern of expression on a high proportion of the IPFP -derived cells and of epitopes commonly abundant on adult stem cells ... Clonal IPFP -derived cells retained the cell surface characteristics of the parent IPFP cells, which were similar to mesenchymal stem cells The clonal populations of IPFP -derived cells appeared ... characterisation of infrapatellar fat padderived cells The rate of proliferation of the IPFP -derived cells in monolayer culture was significantly increased by FGF-2 A comparison of their proliferation...

Ngày tải lên: 09/08/2014, 10:23

... approximately 40 cm3 of packed adipose tissue, fibrous tissue, red blood cells and a small number of nucleated cells An equal volume of digestive enzyme, 0.07% collagenase type 1, composed of several collagenases, ... Mesenchymal stem cell therapy in joint disease Novartis Found Symp 2003, 249:86-96 Zhang HN, Li L, Leng P, Wang YZ, Lv CY: Uninduced adipose- derived stem cells repair the defect of full-thickness ... Alfonso ZC, Fraser JK, Benhaim P, Hedrick MH: Human adipose tissue is a source of multipotent stem cells Mol Biol Cell 2002, 13:4279-4295 13 Peterkofsky B: Bacterial collagenase Methods Enzymol...

Ngày tải lên: 10/08/2014, 23:21

... Isolation of mesenchymal stem cells of fetal or maternal origin from human placenta Stem Cells 22, 1338–1345 Bieback K, Kern S, Kluter H & Eichler H (2004) Critical parameters for the isolation of mesenchymal ... differentiation of human umbilical cord blood -derived mesenchymal stem cells into hepatocytelike cells Biochem Biophys Res Commun 330, 1153–1161 Seo MJ, Suh SY, Bae YC & Jung JS (2005) Differentiation of human ... embryonic stem cells Hepatology 41, 836–846 Banas A, Teratani T, Yamamoto Y, Tokuhara M, Takeshita F, Quinn G, Okochi H & Ochiya T (2007) Adipose tissue -derived mesenchymal stem cells as a source of human...

Ngày tải lên: 30/03/2014, 04:20

... therapeutic role of adiposederived mesenchymal stem cells (ADMSCs) in acute IR injury of the lung has not been investigated Recently, ADMSCs have been reported to have the distinct advantages of being ... Use of Laboratory Animals (NIH publication No Page of 13 85-23, National Academy Press, Washington, DC, USA, revised 1996) Animal Grouping and Isolation of Adipose- Derived Mesenchymal Stem Cells ... parenchyma from IR damage Figure Impact of Adipose- Derived Mesenchymal Stem Cells (ADMSC) Transplantation on the Severity of IR-Induced Lung Parenchymal Injury Number of alveolar sacs and crowded area...

Ngày tải lên: 18/06/2014, 22:20

... there are two well defined types of stem cells – the embryonic stem cells and the adult stem cells The embryonic stem cells, which are found within inner cell mass of the embryonic blastocyst, is ... good source of multipotent adult stem cells is the adipose tissue Cells isolated from these, known as ADSCs (Adipose Derived Stromal Cells) have been shown to possess the property of being multipotent ... is currently being focused on the use of stem cells and precursor cells for this purpose The two types of stem cells that are being targeted for use in tissue regeneration are the Embryonic stem...

Ngày tải lên: 08/11/2015, 17:01

... 2.2 Stem cells in tissue regeneration 2.2.1 Embryonic stem cells 2.2.2 Adult stem cells 2.2.3 Adipose derived stem cells Chapter Chapter v 2.2.4 Hepatic differentiation of adipose derived stem ... of stemness marker genes on gelatin 49 microspheres 4.2.4 Adipogenic and osteogenic differentiation of 49 adipose derived stem cells 4.2.5 Hepatic differentiation of adipose derived stem 51 cells ... Pro-angiogenic activity of adipose derived stem 53 cell – gelatin microsphere constructs 4.3 Discussion 54 4.4 Conclusions 59 Chapter Osteogenic induction of human adipose 60 derived stem cells in a collagen...

Ngày tải lên: 09/09/2015, 11:18

... cases except that of the Rex-1 gene, primer sets span introns Day cells are composed of ES cells and MEF cells grown in ES media containing leukemia inhibitory factor (LIF) These cells express the ... Discussion B Fig Transcription of LCR hypersensitive site in undifferentiated murine embryonic stem cells and in human CD 133+ bone marrow cells (A) Transcription of LCR regions and the bmaj-globin ... the differentiation of hematopoietic stem cells to erythroid cells Transcription of HS3 could be important in maintaining this accessible structure, particularly in light of the fact that RNA...

Ngày tải lên: 07/03/2014, 12:20

... (Table 2) The number of CD4+/CD25+ T cells and of CD3+/CD16/56+ NK-like T cells was always below 5% Like in healthy human individuals the proportion of CD3-/CD16+CD56+ NK cells in the peripheral ... A- Photomicrograph view of the patient -derived cell line of the anastomotic relapse A- Photomicrograph view of the patient -derived cell line of the anastomotic relapse Cells were cultured and ... cytolytic activity of patient -derived PBMNC derived from L1 to L6 and L7 to L9 against K562 cells (A) and autologous In vitro (B) In vitro cytolytic activity of patient -derived PBMNC derived from L1...

Ngày tải lên: 18/06/2014, 15:20

... isolation of relatively pure populations of these cells for in vitro study of the cellular processes in thalassemic stem cells While previous attempts have focused on the cell culture system, in ... The isolated HSCs/CD34+ cells were harvested for phosphoproteomic analysis and cultivation of erythroid cells The cultivation of erythroid cells derived from HSCs/CD34+ cells was performed according ... vitro culture is capable of sustaining a large number of cells, which can confer specific advantages and disadvantages The use of freshly isolated human hematopoietic stem cells from thalassemic...

Ngày tải lên: 18/06/2014, 19:20

... of the adipogenesis of marrow mesenchymal stem cells (MSCs) [7] Results of a previous study showed increased number of small size fat cells in the early steroid-associated ON, that might be derived ... trabecular bone area); fat cells diameter = the total diameter of fat cells in selected field/the number of fat cells in selected field; fat cells area = the area of all fat cells in selected field/(the ... mean fat cells size and fat cells area would be measured with imaging process software Image-Pro Plus 5.1 (Media Cybernetics Inc., Silver Spring, MD) Fat cells density = marrow fat cells number...

Ngày tải lên: 20/06/2014, 00:20

... isolation of relatively pure populations of these cells for in vitro study of the cellular processes in thalassemic stem cells While previous attempts have focused on the cell culture system, in ... The isolated HSCs/CD34+ cells were harvested for phosphoproteomic analysis and cultivation of erythroid cells The cultivation of erythroid cells derived from HSCs/CD34+ cells was performed according ... vitro culture is capable of sustaining a large number of cells, which can confer specific advantages and disadvantages The use of freshly isolated human hematopoietic stem cells from thalassemic...

Ngày tải lên: 20/06/2014, 04:20

... stimulation of endogenous neural progenitor or stem cells and the transplantation of adult -derived neural progenitor and stem cells [39] Self-renewing multipotent neural progenitor and stem cells have ... brain originate from residual stem cells The existence of stem cells in the adult brain suggests that it has the potential for self-repair and that newborn neuronal cells may contribute to the ... progenitor or stem cells locally would represent a strategy to promote regeneration of the diseased and injured nervous system Alternatively, new neuronal cells are generated at sites of degeneration...

Ngày tải lên: 08/08/2014, 17:20

... the induction of MSC adipogenesis Myogenesis Most investigations of myogenesis in adult stem cells are based on a small population of skeletal muscle -derived stem cells, or satellite cells A recent ... mesenchymal stem cells Stem Cells 2004, 22:849-860 Gronthos S, Franklin DM, Leddy HA, Robey PG, Storms RW, Gimble JM: Surface protein characterization of human adipose tissue -derived stromal cells ... critical for self-renewal of human multipotent adipose- derived stem cells Stem Cells 2006, 24:2412-2419 Kleber M, Sommer L: Wnt signaling and the regulation of stem cell function Curr Opin Cell Biol...

Ngày tải lên: 09/08/2014, 10:20

... biology of the prostate: stem cell biology Urology 2003, 62:11-20 Bonkhoff H: Role of the basal cells in premalignant changes of the human prostate: a stem cell concept for the development of prostate ... basal/ stem cells Patch1 and Smo were found highly expressed in the P63+ basal /stem cells of the pCX-shh-IG-injected prostates, being located in the cell membrane of P63+ basal cells in regions of ... P63+ basal /stem cells were transformed into AR+ or ARcancer cells but rarely into ChgA+ neuroendocrine cancer cells Conventional human prostate cancer cells include malignant cells of luminal, basal...

Ngày tải lên: 10/08/2014, 05:21

... According to the hierarchical model of cancer, the origin of the cancer stem cells may be long-lived somatic stem cells Therefore, markers of “normal” stem cells are being sought with the expectation ... hsa-miR-935 SP cells are enriched for markers of HSCs To examine whether SP cells are enriched for characteristics of stem cells compared to the non-SP cells, we further characterized the SP cells from ... carcinogenesis and differentiation of normal stem cells strongly suggest that miRNA may be involved in the transformation of normal stem cells into cancer stem cells Therefore, screening for differences...

Ngày tải lên: 10/08/2014, 10:20

... Page of 11 Figure Mesenchymal stem cells (MSCs) inhibit the proliferation of CD4 + T cells in vitro by induction of nitric oxide and prostaglandin E2 (PGE2) (a) CD4+ T cells, in the presence of ... tolerance with human adipose- derived mesenchymal stem cells Arthritis Rheum 2009, 60:1006-1019 Mao F, Xu WR, Qian H, Zhu W, Yan YM, Shao QX, Xu HX: Immunosuppressive effects of mesenchymal stem cells ... immunomodulatory activity of human bone marrow mesenchymal stem cells Stem Cells 2006, 24:386-398 Meisel R, Zibert A, Laryea M, Gobel U, Daubener W, Dilloo D: Human bone marrow stromal cells inhibit allogeneic...

Ngày tải lên: 12/08/2014, 11:23

... chondrogenic potential of fibrous synovium- and adipose synovium -derived cells compared with subcutaneous fatderived cells: distinguishing properties of mesenchymal stem cells in humans Arthritis Rheum ... http://arthritis-research.com/content/12/6/R206 Page of 13 Figure Synovial MSCs affect gene profile of nucleus pulposus cells in co-culture system (a) Morphology of mono-culture of rat nucleus pulposus cells and human synovial MSCs ... gene profile of rat nucleus pulposus cells in mono-culture and in co-culture with human MSCs (d) Number of altered rat genes seven days after coculture of rat nucleus pulposus cells with human...

Ngày tải lên: 12/08/2014, 15:22

... synthesis of blood cells Erythrocytes, leukocytes, platelets and other blood cells are produced via haematopoiesis by the haemapoietic stem cells found within the medullary cavity of long bones ... significant number of cells for use in various applications [21-23] Mesenchymal Stem Cell (MSC) Bone marrow was the first tissue described as a source of plastic-adherent, fibroblast-like cells that ... Optimization of PEMF parameters 64 3.1.14 Statistical analysis 65 3.2 Results 66 3.2.1 Demonstration of osteogenic potential 66 3.2.2 Proof of concept of PEMF...

Ngày tải lên: 09/09/2015, 17:52