spinal applications of adipose derived stem cells

... mesenchymal stem cells Stem Cells Dev 2008, 17, 681-693 Kang SK, Lee DH, Bae YC, Kim HK, Baik SY, Jung JS Improvement of neurological deficits by intracerebral transplantation of human adipose tissue -derived ... Immunophenotype of human adiposederived cells: temporal changes in stromal-associated and stem cell-associated markers Stem Cells 2006, 24, 376-385 26 Muir WW 3rd, Wiese AJ, March PA Effects of morphine, ... with isoflurane Am J Vet Res 2003, 64, 1155-1160 27 Ogawa R The importance of adipose- derived stem cells and vascularized tissue regeneration in the field of tissue transplantation Curr Stem Cell...

Ngày tải lên: 07/08/2014, 23:22

... therapeutic role of adiposederived mesenchymal stem cells (ADMSCs) in acute IR injury of the lung has not been investigated Recently, ADMSCs have been reported to have the distinct advantages of being ... Use of Laboratory Animals (NIH publication No Page of 13 85-23, National Academy Press, Washington, DC, USA, revised 1996) Animal Grouping and Isolation of Adipose- Derived Mesenchymal Stem Cells ... parenchyma from IR damage Figure Impact of Adipose- Derived Mesenchymal Stem Cells (ADMSC) Transplantation on the Severity of IR-Induced Lung Parenchymal Injury Number of alveolar sacs and crowded area...

Ngày tải lên: 18/06/2014, 22:20

... approximately 40 cm3 of packed adipose tissue, fibrous tissue, red blood cells and a small number of nucleated cells An equal volume of digestive enzyme, 0.07% collagenase type 1, composed of several collagenases, ... Mesenchymal stem cell therapy in joint disease Novartis Found Symp 2003, 249:86-96 Zhang HN, Li L, Leng P, Wang YZ, Lv CY: Uninduced adipose- derived stem cells repair the defect of full-thickness ... with autologous adipose- tissue -derived stem cells: a case series Journal of Medical Case Reports 2011 5:296 Submit your next manuscript to BioMed Central and take full advantage of: • Convenient...

Ngày tải lên: 10/08/2014, 23:21

... mesenchymal stem cells of fetal or maternal origin from human placenta Stem Cells 22, 1338–1345 Bieback K, Kern S, Kluter H & Eichler H (2004) Critical parameters for the isolation of mesenchymal stem cells ... evidence of transdifferentiation by MET in the process of hepatic differentiation of AT-MSCs No previous report has demonstrated evidence of transdifferentiation of committed adult stem cells In ... embryonic stem cells Hepatology 41, 836–846 Banas A, Teratani T, Yamamoto Y, Tokuhara M, Takeshita F, Quinn G, Okochi H & Ochiya T (2007) Adipose tissue -derived mesenchymal stem cells as a source of...

Ngày tải lên: 30/03/2014, 04:20

... there are two well defined types of stem cells – the embryonic stem cells and the adult stem cells The embryonic stem cells, which are found within inner cell mass of the embryonic blastocyst, is ... good source of multipotent adult stem cells is the adipose tissue Cells isolated from these, known as ADSCs (Adipose Derived Stromal Cells) have been shown to possess the property of being multipotent ... of stem cells and precursor cells for this purpose The two types of stem cells that are being targeted for use in tissue regeneration are the Embryonic stem cells and the Adult stem cells The...

Ngày tải lên: 08/11/2015, 17:01

... bioreactors Conflicts of interest The authors have declared that no conflict of interest exists References Becker EW The roles of ATP in the dynamics of the actin filaments of the cytoskeleton ... Bongso A An efficient and safe xeno-free cryopreservation method for the storage of human embryonic stem cells Stem Cells 2004;22(5):779-89 10 Speicher MR, Ballard SG, Ward DC Karyotyping human ... and m-FISH (Figure 3) of metaphase spreads Hence, the results of this study could have useful implications for the salvage of hESC culture during infrequent occurrences of incubator break-down...

Ngày tải lên: 31/10/2012, 16:57

... cases except that of the Rex-1 gene, primer sets span introns Day cells are composed of ES cells and MEF cells grown in ES media containing leukemia inhibitory factor (LIF) These cells express the ... the differentiation of hematopoietic stem cells to erythroid cells Transcription of HS3 could be important in maintaining this accessible structure, particularly in light of the fact that RNA ... both hematopoi- Fig Sequential activation of globin gene transcription during in vitro erythroid differentiation of murine embryonic stem cells PCR analysis of DNase I treated and reverse-transcribed...

Ngày tải lên: 07/03/2014, 12:20

... expansion of human embryonic stem cells, and more recently, of human induced pluripotent stem cells The exploration and realization of the incredible potential of these pluripotent stem cells for ... culture, expansion and manipulation of human pluripotent stem cells The culmination of two events has enabled the production of human embryonic stem cells: the birth of the first IVF test-tube baby ... translational research in the field of embryonic and induced pluripotent stem cells It commences with practical aspects of the derivation and growth of human embryonic stem cells from inner cell mass...

Ngày tải lên: 22/03/2014, 09:20

... human [16,17] and mouse embryonic stem cells [18-20] The unique patterns of miRNA expression in embryonic stem cells suggest they are involved in maintaining "stemness" Identifying mRNAs that ... expression profiling We assessed global hES gene expression profiles on separate passages of cells from different hES cell lines, EB samples at different time points, and types of adult cells, HUVEC, ... Adult cells and EB shared genes (B) hES cells upregulated genes (C) EB upregulated genes (D) Adult cells upregulated genes hESC cells EB adult cells Figure supervised hierarchical clustering of...

Ngày tải lên: 18/06/2014, 15:20

... A- Photomicrograph view of the patient -derived cell line of the anastomotic relapse A- Photomicrograph view of the patient -derived cell line of the anastomotic relapse Cells were cultured and ... cytolytic activity of patient -derived PBMNC derived from L1 to L6 and L7 to L9 against K562 cells (A) and autologous In vitro (B) In vitro cytolytic activity of patient -derived PBMNC derived from L1 ... The presence of CD3+ and CD45+ cells was used as an indicator of T cell infiltration and the prevalence of CD4+, CD8+, CD56+ cells as indicators of T helper, T cytotoxic and NK cells, respectively...

Ngày tải lên: 18/06/2014, 15:20

... isolation of relatively pure populations of these cells for in vitro study of the cellular processes in thalassemic stem cells While previous attempts have focused on the cell culture system, in ... The isolated HSCs/CD34+ cells were harvested for phosphoproteomic analysis and cultivation of erythroid cells The cultivation of erythroid cells derived from HSCs/CD34+ cells was performed according ... the elucidation of signal transduction pathways, the understanding of the mechanism of disease progression, and the development of therapeutic applications [12] The phosphoproteome of HSCs in HbE/b-thalassemic...

Ngày tải lên: 18/06/2014, 19:20

... of the adipogenesis of marrow mesenchymal stem cells (MSCs) [7] Results of a previous study showed increased number of small size fat cells in the early steroid-associated ON, that might be derived ... trabecular bone area); fat cells diameter = the total diameter of fat cells in selected field/the number of fat cells in selected field; fat cells area = the area of all fat cells in selected field/(the ... mean fat cells size and fat cells area would be measured with imaging process software Image-Pro Plus 5.1 (Media Cybernetics Inc., Silver Spring, MD) Fat cells density = marrow fat cells number...

Ngày tải lên: 20/06/2014, 00:20

... isolation of relatively pure populations of these cells for in vitro study of the cellular processes in thalassemic stem cells While previous attempts have focused on the cell culture system, in ... The isolated HSCs/CD34+ cells were harvested for phosphoproteomic analysis and cultivation of erythroid cells The cultivation of erythroid cells derived from HSCs/CD34+ cells was performed according ... the elucidation of signal transduction pathways, the understanding of the mechanism of disease progression, and the development of therapeutic applications [12] The phosphoproteome of HSCs in HbE/b-thalassemic...

Ngày tải lên: 20/06/2014, 04:20

... stimulation of endogenous neural progenitor or stem cells and the transplantation of adult -derived neural progenitor and stem cells [39] Self-renewing multipotent neural progenitor and stem cells have ... brain originate from residual stem cells The existence of stem cells in the adult brain suggests that it has the potential for self-repair and that newborn neuronal cells may contribute to the ... progenitor or stem cells locally would represent a strategy to promote regeneration of the diseased and injured nervous system Alternatively, new neuronal cells are generated at sites of degeneration...

Ngày tải lên: 08/08/2014, 17:20

... the induction of MSC adipogenesis Myogenesis Most investigations of myogenesis in adult stem cells are based on a small population of skeletal muscle -derived stem cells, or satellite cells A recent ... critical for self-renewal of human multipotent adipose- derived stem cells Stem Cells 2006, 24:2412-2419 Kleber M, Sommer L: Wnt signaling and the regulation of stem cell function Curr Opin Cell Biol ... mesenchymal stem cells Stem Cells 2004, 22:849-860 Gronthos S, Franklin DM, Leddy HA, Robey PG, Storms RW, Gimble JM: Surface protein characterization of human adipose tissue -derived stromal cells...

Ngày tải lên: 09/08/2014, 10:20

... number of cells was determined spectrophotometrically entiation of human mesenchymal transcripts Expression of BMP-2 and chordinstem cells during osteogenic differentiation of human mesenchymal stem ... suppression of chordin led to an increase in the bioavailability of endogenously produced BMP-2 to drive the differentiation of Page of (page number not for citation purposes) Effect of chordin ... extrapolated to cells from a large population of donors because there is consistently a significant and large increase in expression of osteogenic markers, as a result of chordin knockdown in cells from...

Ngày tải lên: 09/08/2014, 10:23

... staining of IPFPderived cells It was therefore interesting that they showed a pattern of expression on a high proportion of the IPFP -derived cells and of epitopes commonly abundant on adult stem cells ... Clonal IPFP -derived cells retained the cell surface characteristics of the parent IPFP cells, which were similar to mesenchymal stem cells The clonal populations of IPFP -derived cells appeared ... characterisation of infrapatellar fat padderived cells The rate of proliferation of the IPFP -derived cells in monolayer culture was significantly increased by FGF-2 A comparison of their proliferation...

Ngày tải lên: 09/08/2014, 10:23

... mesenchymal stem cells for osteoarthritis therapy Nat Clin Pract Rheumatol 2008, 4:371-380 Estes BT, Wu AW, Guilak F: Potent induction of chondrocytic differentiation of human adipose- derived adult stem ... arcades of Benninghoff [29] and by the appearance of hypertrophic cells Analyses of lacunae surface areas in the different growth plate zones revealed mean lacunae surface areas ± SD of 100.8 ... differentiation of cultured human mesenchymal stem cells from marrow Tissue Eng 1998, 4:415-428 35 Mueller MB, Tuan RS: Functional characterization of hypertrophy in chondrogenesis of human mesenchymal stem...

Ngày tải lên: 09/08/2014, 14:22

... differentiation of size-sieved stem cells into electrically active neural cells Stem Cells 2002, 20(6):522-529 Tzeng SF, Tsai MJ, Hung SC, Cheng H: Neuronal morphological change of size-sieved stem cells ... differentiation of human mesenchymal stem cells into insulin producing cells J Biomed Mater Res A 2008, 86(4):1097-1105 Chao KC, Chao KF, Fu YS, Liu SH: Islet-like clusters derived from mesenchymal stem cells ... and survival of adherent cells [17] FN and LAM constitute the major ECM of pancreas islet cells Laminin-1 has been reported to promote the differentiation of fetal mouse pancreatic b -cells [18]...

Ngày tải lên: 10/08/2014, 05:21