experimental procedures for the purification and characterization of the arabinanases and galactanases
Occurrence of Polyhydroxyalkanoate as Temporal Carbon Storage Material in Activated Sludge during The Removal of Organic Pollutants
... was added to it, the tube was shaken, and, the methyl ester of PHA monomers in the chloroform layer was collected The chloroform phase was washed again with 0.5mL water, and then injected to ... PHA The copolymer composed of 81% of 3HB and 19% of 3-hydroxyvalerate (3HV) (Sigma-Aldrich Japan K.K., Japan) was used as the standard of 3HV unit in PHA These standards were also digested and ... (5) (6) The CRP values were determined for the first reaction tank except for MUCT reactor As for MUCT, CRP value was calculated for the first anaerobic and first anoxic tanks The unit of measured...
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Occurrence of Tetracycline-Resistant and Tetracycline- Degrading Bacteria in Wastewater Treatment Plant Effluent and Environmental Water Systems
... growth in each sample for the analysis of the growth of tetracycline-resistant or tetracycline-degrading bacteria and the mechanism of tetracycline degradation MATERIALS AND METHODS Water samples ... under a microscope, and the size and shape of microbial cells were studied to determine whether the colonies were bacterial or fungal RESULTS AND DISCUSSION The changes in the tetracycline concentration ... affected the results of this study Tetracycline degrading ability was exhibited by of 15 colonies of resistant bacteria isolated from the Unoke River water samples, and the density of these tetracycline-degrading...
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Tài liệu WATER POLLUTION BY INSECTICIDES IN AN AGRICULTURAL RIVER .BASIN. I. OCCURRENCE OF INSECTICIDES IN RIVER AND TREATED MUNICIPAL WATER pdf
... with chloroform for 24 hr in a Soxhlet apparatus The chloroform was then cvaporated, the rcsiduc taken up in acetonitrile, and the insecticides partitioned into petroleum ether The ether fraction ... 62 km2) of the basin are in the William B Bankhead National Forest This area is on the slopes of the divide in the southwest portion of the watershed In 1961, 1,210 acres (490 ha) of the forest ... product and an impurity in formulated 2,4,5-T Along the course of the West Fork of Flint Creek, the principal stream draining the forest, there are approximately 36 miles (58 km) between the forest...
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Tài liệu Báo cáo khoa học: The occurrence of hemocyanin in Hexapoda docx
... genedoc 2.7 [41] The tools provided with the ExPASy Molecular Biology Server of the Swiss Institute of Bioinformatics (http:// www.expasy.org) were used for the analyses of DNA and amino acid sequences ... and AccuPrime Taq DNA Polymerase (Invitrogen, Karlsruhe, Germany) according to the manufacturer’s instructions For control of the efficiency of the cDNA synthesis, b-actin was amplified using the ... indication of the presence of hemocyanin (data not shown) The notion of the absence of hemocyanins from Holometabola is corroborated by the fact that no hemocyanin sequences could be identified in the...
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Tài liệu Báo cáo khoa học: The molecular surface of proteolytic enzymes has an important role in stability of the enzymatic activity in extraordinary environments pptx
... wild-type ALP I On the other hand, the Tm of D266N/Y269A-ALP I increased by only °C The denaturation temperature indicates the stability of the structure of the protein The difference of the increments ... 60% of the original activity after h, and 30% after h The D266NALP I showed 40 and 20% of the original activity after h and h of incubation, respectively The stability of Y269A/A271T/Q272R- and ... His, the side chain is larger The mutation seemed to fill in the gap between surrounding residues of the depression, and the side chain of the mutated residue might restrain the mobility of the...
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Tài liệu Báo cáo Y học: Evolution of the enzymes of the citric acid cycle and the glyoxylate cycle of higher plants pdf
... structure, and that some of the subunits of PDH, OGDH and OADH are related The functional and evolutionary relationships between the subunits of these enzymes are somewhat complicated In a nutshell, the ... [21,22] and in Euglena mitochondria [39] The E2 subunit of PDH contains the dihydrolipoamide transferase activity The mitochondrial form of the E2 subunit for PDH is related to the E2 subunits of ... analysis of the evolution of these enzymes than we have performed here However, our aim was not to perform an exhaustive analysis but to obtain an overview of the patterns of similarity for the enzymes...
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Tài liệu Báo cáo Y học: Interallelic recombination is probably responsible for the occurrence of a new as1-casein variant found in the goat species potx
... F, while components e and d seem to be in common with the A variant Therefore, band b represents the only component specific to the M variant The intensities of the bands in the MF pattern indicate ... indicate the position of the introns The boxes indicate amino-acid substitutions skipping of three successive exons during the course of the processing of the primary transcripts [9] At the peptide ... wonder whether the donor allele of the 5¢ part is the F allele or another allele belonging to the same B allelic lineage (excluding B1 and C), as they share the same simplified haplotype formula,...
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Báo cáo khoa học: The occurrence of riboflavin kinase and FAD synthetase ensures FAD synthesis in tobacco mitochondria and maintenance of cellular redox status docx
... (B) Dependence of the rate of FMN synthesis on Rf concentrations (C,D) Time courses of FAD synthesis and Rf appearance after addition of either lM or 50 lM FMN Values are the mean of three replicates ... [2022,27,28,3342] The dependence of the rate of FMN synthesis on Rf concentration shows saturation characteristics with a sigmoidal shape The S0.5 value of RK is in the same order of magnitude as the Km ... described in Experimental procedures The y-axis represents the avin transport synthesis rates expressed as pmolặmin)1ặmg)1 protein Values are the mean of three replicates ( SD) performed using the same...
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Low Occurrence of Tuberculosis Drug Resistance among Pulmonary Tuberculosis Patients froman Urban Setting, with a Long-Running DOTS Programin Zambia docx
... Geneva, Switzerland, 4th edition, 2008 [10] S A Petroff., “A new and rapid method for the isolation and cultivation of tubercle bacilli directly form the sputum and feces,” The Journal of Experimental ... levels of any resistance to first-line drugs (8.8%) in Ndola, and for the first time systematically investigated and documented absence of resistance to second-line drugs (OFL and KAN) These levels of ... results and treatment outcome at the end of treatment The study registers were checked against the clinic TB registers at the end of the study period to complete any missing data and for verification...
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Báo cáo khoa học: MAP2 prevents protein aggregation and facilitates reactivation of unfolded enzymes ppt
... a-crystallin, TRiC, thermosome and many other chaperones [32] Deletion of the tail abolishes the chaperone function of these proteins The importance of the acidic residue-rich tails of tubulin in its ... about 120 residues of the C-terminus of MAP2 (Fig 5) Therefore, the role of a large portion of the MAP2 remains unknown To pinpoint the structural elements responsible for the observed chaperone-like ... at their C-terminal region, which constitutes the tubulin-binding domain Each of these repeats has more homology to the corresponding repeats of the other two than to the other repeats in the...
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Báo cáo khoa học: b3-Adrenoceptor knockout in C57BL/6J mice depresses the occurrence of brown adipocytes in white fat pptx
... occurrence of brown adipocytes in WAT of C57BL/6J mice at 24 °C and after cold exposure We therefore measured (a) the density of multilocular cells in WAT, and (b) the levels of UCP1 mRNA and protein ... activity of PPARc, of the thyroid hormone receptors and of UCP1 in BAT The expression of PGC-1 mRNA was measured in interscapular BAT and in genital WAT, using the same Northern blots as for UCP1 ... °C) for either two or 10 days The fat pads along the uterus and fallopian tube (parametrial fat) and those around the ovaries (periovarian) were dissected and pooled They are referred to in the...
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Báo cáo Y học: Implications of the simultaneous occurrence of hepatic glycolysis from glucose and gluconeogenesis from glycerol pdf
... determination of the rate of glycolysis from glucose [2] and 1.0 lCi [2-3H]glucose for determination of the rate of glucose phosphorylation [1] For the measurement of glycerol metabolism, the incubation ... concentrations fell The extent of these changes and the rate of glycerol uptake and glucose synthesis were dependent on the initial concentration of added substrate and were maximal by mM (Table ... weight))1 (n 5) Thus in the presence of glycerol, there was an apparent net synthesis of glucose of 0.62 0.05 lmolámin)1á(g wet weight))1 The rate of glycogen synthesis of 0.13 0.01 lmolámin)1á(g...
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Báo cáo Y học: Changes in ultrastructure and the occurrence of permeability transition in mitochondria during rat liver regeneration ppt
... observed when the mitochondrial preparation was performed either in the presence or in the absence of ruthenium red and EDTA in isolation buffer Matrix proteins release assay For the assay of the in ... succinate and phosphate Mitochondria were suspended in the swelling medium and the absorbance of the mitochondrial suspension as a function of time was monitored either in the absence or in the presence ... PH, the activities of the enzymes released in the supernatant (1.8 ± 0.1 and 0.8 ± 0.04% of the total mitochondrial activity of GDH and AAT, respectively), were as low as those found in the supernatant...
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Báo cáo khóa học: Cloning and expression of murine enzymes involved in the salvage pathway of GDP-L-fucose ppt
... pyrophosphorylase The concentration of the double labelled probe was 200 nM for the long variant of fucokinase and pyrophosphorylase, and 300 nM for the short fucokinase splice variant Relative amounts of the ... levels of L-fucokinase and pyrophosphorylase The primer and probe sequences and their positions in the mRNA sequence, for GDP-L-fucose pyrophosphorylase and the short and long splice variants of ... nM/300 nM (forward/reverse) for the long splice variant of fucokinase, 900 nM/900 nM (forward/ reverse) for the short splice variant of fucokinase, and 300 nM/900 nM (forward/reverse) for pyrophosphorylase...
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Báo cáo khoa học: "Utilizing Co-Occurrence of Answers in Question Answering" pdf
... some of the questions in the series are mapped to the attributes of the topic and thus grouped together according to the attribute clusters Then questions in the same group are processed together ... Proceedings of the 42nd ACL 2004 The Authors wish to thank BBN for the use of NE tagging software IdentiFinder, CIIR at University of Massachusetts for the use of Inquery search engine, Stanford University ... “Organization”, “Event” and “Things” The topic can be viewed as an object and the series of questions can be viewed as asking for the attributes of the object In this point of view, to find out...
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báo cáo hóa học: " Occurrence of post traumatic stress symptoms and their relationship to professional quality of life (ProQoL) in nursing staff at a forensic psychiatric security unit: a cross-sectional study" potx
... TP and KN conceived and designed the study CL collected the data CL and TP performed statistical analysis and drafted the manuscript All authors revised the manuscript critically and approved the ... in the central and northern parts of Norway with about 1.1 million inhabitants The unit consists of wards with a total number of 21 beds One of the wards (ward F) is specialised to serve offenders ... consulted and the committee considered this study not liable for formal approval as only staff members participated and the study was part of the hospitals internal actions to initiate Page of (page...
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Báo cáo sinh học: " Comparison of amplification enzymes for Hepatitis C Virus quasispecies analysis" docx
... of the 176 bp E2-HVR1 is indicated with arrows MW represents the 100 base pair DNA molecular weight marker Below each lane is the result of testing of the same dilution of the standard with the ... (W20) of pegylated IFN plus ribavirin therapy The box plots represent the means and ranges of the HMR and complexity scores for 400 HVR1 clones amplified by each polymerase, for a total of 800 ... dividing the distance in millimeters (mm) from the origin of the gel to the heteroduplex by the distance in mm from the origin to the homoduplex control In cases where both strands of the heteroduplex...
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báo cáo hóa học:" The occurrence of osteoarthritis at a minimum of ten years after reconstruction of the anterior cruciate ligament" pdf
... reached for follow-up There were 11 women and 17 men The mean age at the time of surgery was 30.5 years (range 16 to 42) The left side was involved in 58% and the right in 42% of the patients The ... risk of further knee injury due to the degree of laxity and the desired level of activity [27] In order to minimise the development of arthrofibrosis, reconstruction was carried out only after the ... to the contralateral side (Table and 4) The Fairbank classification showed an increase in osteoarthritis of grade in 52% of the patients, 35% of the patients had an increase of grades and 3% of...
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Báo cáo lâm nghiệp: "On the occurrence of web-spinning sawflies of the genus Cephalcia (Hymenoptera, Pamphiliidae) in the Czech Republic" potx
... from the end of April to the end of October and emptied in two-week or one month intervals during the period 2001–2006 The sawflies were determined according to the key of Viitasaari (2002) and the ... regions of the Czech Republic; (i) Euhercynicum (the Šumava Mts., Slavkovský les, Český les, Brdy Upland and Žďárské vrchy Upland), (ii) Sudeten (the Krkonoše Mts and the Krušné hory Mts.) and (iii) ... periods of the Cephalcia species flight activities (cf Martinek 1988) The flight activity started in the studied localities at the end of May or at the beginning of June depending on the actual weather...
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