characterization of human adipose derived stem cells using flow cytometry

... Isolation of mesenchymal stem cells of fetal or maternal origin from human placenta Stem Cells 22, 1338–1345 Bieback K, Kern S, Kluter H & Eichler H (2004) Critical parameters for the isolation of mesenchymal ... differentiation of human umbilical cord blood -derived mesenchymal stem cells into hepatocytelike cells Biochem Biophys Res Commun 330, 1153–1161 Seo MJ, Suh SY, Bae YC & Jung JS (2005) Differentiation of human ... embryonic stem cells Hepatology 41, 836–846 Banas A, Teratani T, Yamamoto Y, Tokuhara M, Takeshita F, Quinn G, Okochi H & Ochiya T (2007) Adipose tissue -derived mesenchymal stem cells as a source of human...

Ngày tải lên: 30/03/2014, 04:20

... Immunophenotype of human adiposederived cells: temporal changes in stromal-associated and stem cell-associated markers Stem Cells 2006, 24, 376-385 26 Muir WW 3rd, Wiese AJ, March PA Effects of morphine, ... HE Stem cell therapy for neurologic disorders: therapeutic potential of adipose- derived stem cells Curr Drug Targets 2005, 6, 57-62 33 Satake K, Lou J, Lenke LG Migration of mesenchymal stem cells ... with isoflurane Am J Vet Res 2003, 64, 1155-1160 27 Ogawa R The importance of adipose- derived stem cells and vascularized tissue regeneration in the field of tissue transplantation Curr Stem Cell...

Ngày tải lên: 07/08/2014, 23:22

... there are two well defined types of stem cells – the embryonic stem cells and the adult stem cells The embryonic stem cells, which are found within inner cell mass of the embryonic blastocyst, is ... good source of multipotent adult stem cells is the adipose tissue Cells isolated from these, known as ADSCs (Adipose Derived Stromal Cells) have been shown to possess the property of being multipotent ... of stem cells and precursor cells for this purpose The two types of stem cells that are being targeted for use in tissue regeneration are the Embryonic stem cells and the Adult stem cells The...

Ngày tải lên: 08/11/2015, 17:01

... cryopreserved human adipose- derived stem cells Cryobiology 2008, 57:18-24 Chen MY, Lie PC, Li ZL, Wei X: Endothelial differentiation of Wharton’s jelly -derived mesenchymal stem cells in comparison ... endothelial cells enhance osteogenesis and vascularization of mesenchymal stem cells Cells Tissues Organs 2009, 190:185-193 Rodbell M: The metabolism of isolated fat cells IV Regulation of release of ... High-potential human mesenchymal stem cells Stem Cells Dev 2005, 14:70-80 Aust L, Devlin B, Foster SJ, Halvorsen YD, Hicok K, du Laney T, Sen A, Willingmyre GD, Gimble JM: Yield of human adipose- derived...

Ngày tải lên: 10/08/2014, 10:20

... Reliable procurement of autologous ADSC • Characterization of cells in in vitro culture plastic environment • Characterization of cells in in vitro 3-D environment • Characterization of cells in in vivo ... characterization of other potential sources of stem cells which might avoid these issues altogether 1.1.2.1 Adult stem cells Stem cells are loosely defined as self-renewing progenitor cells that can ... that cells actually thrive in One of the promising adult stem cell sources is a culture plastic-adherent cell population from the human adipose tissue In order to bring adipose derived stem cells...

Ngày tải lên: 12/09/2015, 09:55

... expression profile of DCs, we suspected that LV infection might also affect DC activation of T cells To test this, we set up an in vitro immunity assay using co-culture of human DCs and naïve T cells ... dilutions of DCs, from 10,000 cells per well to 313 cells per well, with × 105 allogeneic CD4 T cells in a 96-well U-bottomed plate in a total volume of 200 µl for days The proliferation of T cells ... function of DCs Polarization of naïve Th cells into Th1 cells is critical for the induction of cellular immunity against intracellular pathogens and cancer cells The observed impairment of the...

Ngày tải lên: 13/08/2014, 13:20

... Validation of selected genes by flow cytometry and immunocytochemistry Validation of selected genes by flow cytometry and immunocytochemistry (a) Flow cytometry analysis of prostate cancer cells co-stained ... signature Stem cells Stem cells (c) Committed basal Committed basal Stem Committed cells basal (d) Malignancy signature Tumour Birnie et al R83.3 Gleason included Benign Figure Distinctive stem cell ... level using a combination of flow cytometry and immunocytochemistry (Figure 4) Using antibodies to CD133 and NF-κB on primary tumor cultures demonstrated that both progenitor and stem cells expressed...

Ngày tải lên: 14/08/2014, 08:21

... Isolation of HPTC and characterization of isolated and commercial HPTC 50 4.1.1 Isolation of HPTC 50 4.1.2 Characterization of HPTC by immunoblotting 51 4.1.3 Characterization of ... proximal tubular cells of human origin Therefore, human primary renal proximal tubular cells (HPTC) would be most suitable for such applications However, the application of primary human cells is also ... transfection The percentage of dead cells was calculated by expressing total number of PI-stained cells as a percentage of total number of DAPI-stained cells which were counted using Image J 1.4 In...

Ngày tải lên: 09/09/2015, 10:19

... 2.2 Stem cells in tissue regeneration 2.2.1 Embryonic stem cells 2.2.2 Adult stem cells 2.2.3 Adipose derived stem cells Chapter Chapter v 2.2.4 Hepatic differentiation of adipose derived stem ... osteogenic differentiation of 49 adipose derived stem cells 4.2.5 Hepatic differentiation of adipose derived stem 51 cells 4.2.6 Pro-angiogenic activity of adipose derived stem 53 cell – gelatin ... by minimally invasive means which contain large numbers of stem cells 2.2.3 Adipose derived stem cells ADSCs are adult stem cells found in adipose tissues with very similar characteristics to BMSCs...

Ngày tải lên: 09/09/2015, 11:18

... approximately 40 cm3 of packed adipose tissue, fibrous tissue, red blood cells and a small number of nucleated cells An equal volume of digestive enzyme, 0.07% collagenase type 1, composed of several collagenases, ... Mesenchymal stem cell therapy in joint disease Novartis Found Symp 2003, 249:86-96 Zhang HN, Li L, Leng P, Wang YZ, Lv CY: Uninduced adipose- derived stem cells repair the defect of full-thickness ... Alfonso ZC, Fraser JK, Benhaim P, Hedrick MH: Human adipose tissue is a source of multipotent stem cells Mol Biol Cell 2002, 13:4279-4295 13 Peterkofsky B: Bacterial collagenase Methods Enzymol...

Ngày tải lên: 10/08/2014, 23:21

... publication using Adobe Photoshop software (Adobe, http://www.adobe.com) ii Flow cytometry analyses of cell phenotypes The phenotype of in vitro-generated MDSC was examined for expression of myeloid, ... (Sigma) then analyzed by flowcytometry Nitrites were measured in supernatants of cells cultured × 105 cells/ mL overnight in complete medium using Promega’s Griess Reagent System (Madison, WI) per ... analyzed on a FACSCalibur flow cytometry using CellQuestPro software and collecting 50,000 live leukocyte events CD33+HLA-DRlowHIF1a+ cells were found to be 15.78-16.23% of myeloid cells in cancer patients...

Ngày tải lên: 18/06/2014, 19:20

... the creation of human embryonic stem cells J Clin Invest 2004, 114:1184-1186 Voullaire L, Slater H, Williamson R, Wilton L: Chromosome analysis of blastomeres from human embryos by using comparative ... substantial ethical obstacle for the advancement of human ESC research With the vast therapeutic promise of human ESC seen against the destruction of human embryos required to realize such aspirations, ... stage, a human embryo at 2–3d post fertilization consists of just 4–8 cells, all of which are totipotent In contrast to the pluripotent cells obtained from the blastocyst ICM, one or two of these...

Ngày tải lên: 13/08/2014, 23:20

... the topic of this thesis 2.1 Embryonic stem cells Embryonic stem cells (ESCs) are pluripotent cells derived from the inner cell mass of the blastocyst Pluripotent diploid ESCs have been derived ... neonatal stem cells come in between embryonic and adult stem cells in the developmental continuum Studies of these cells have provided information and insights into the biology of stem cells in ... unless autologous cells are used The major obstacle of using autologous cells is to obtain sufficient number of cells, which may potentially be solved by the reprogramming of somatic cells into iPSCs...

Ngày tải lên: 11/09/2015, 10:02

... Vainzof M, Zatz M: SJL dystrophic mice express a significant amount of human muscle proteins following systemic delivery of human adipose- derived stromal cells without immunosuppression Stem Cells ... new potential source of pluripotent cells for regenerative medicine The identification of niches of tissue-specific stem cells capable of replacing damaged differentiated cells in the hFTs may ... Discussion The possibility of using stem cells for regenerative medicine has opened a new field of investigation to find the best sources for obtaining multipotent stem cells, in particular through...

Ngày tải lên: 18/06/2014, 15:20

... staining of IPFPderived cells It was therefore interesting that they showed a pattern of expression on a high proportion of the IPFP -derived cells and of epitopes commonly abundant on adult stem cells ... Clonal IPFP -derived cells retained the cell surface characteristics of the parent IPFP cells, which were similar to mesenchymal stem cells The clonal populations of IPFP -derived cells appeared ... characterisation of infrapatellar fat padderived cells The rate of proliferation of the IPFP -derived cells in monolayer culture was significantly increased by FGF-2 A comparison of their proliferation...

Ngày tải lên: 09/08/2014, 10:23

... retroviral vectors (that enables infection of a wide variety of cells) have revealed differences in the efficiency of single round infection in cells of differing types and species [11,12] Therefore, ... x 105 Figure cells with an HIV-EGFP vector at a moi = 0.5 Infection of parental and mutant HeLa cells (30-2 and 42-7) Infection of parental and mutant HeLa cells (30-2 and 42-7) cells with an ... parental and mutant cells and extent of HIV Growth Growth rates of parental and mutant cells and extent of HIV integration (A) Growth rates Parental and mutant 30-2 and 42-7 cells were seeded and...

Ngày tải lên: 13/08/2014, 05:22

... percentage ratio of the hematocrite value of the final loaded cells to that of the initial packed cells, both measured using equal suspension volumes In vitro characterization of pravastatin loaded ... total amount of pravastatin entrapped in 0.1 ml of the final packed erythrocytes Efficiency of entrapment The percentage of the loaded amount of pravastatin to the total amount of that added ... delivery system of primaquine: in vitro characterization J Microencapsul 1992; 9(3):357-364 34 Hamidi M, Tajerzadeh H, Dehpour AR, Rouini MR, Ejtemaee-Mehr S In vitro characterization of human intact...

Ngày tải lên: 25/10/2012, 11:10

... biochemical characterization of human ACMSD Results Cloning of ACMSD transcripts The complete coding sequence of human ACMSD was obtained from reverse-transcribed human kidney Human ACMSD RNA, by using ... recombinant isoforms Lane M, molecular mass standards production of the human recombinant isoforms might be enhanced by the presence of metal ions in the growth medium We found that addition of 200 ... set in the presence of increasing molar amounts of the other isoform (1 : 1, : 1000 and : 10 000) Detection of ACMSD I was not influenced by the presence of up to Recombinant human ACMSD I was purified...

Ngày tải lên: 19/02/2014, 02:20