isolation expansion and characterization of human islet derived progenitor cells

... Isolation of HPTC and characterization of isolated and commercial HPTC 50 4.1.1 Isolation of HPTC 50 4.1.2 Characterization of HPTC by immunoblotting 51 4.1.3 Characterization of ... containing 0.1 mM of 3-isobutyl-1-methylxanthine (IBMX) (Merck) and exposure of cells to 100 nM and 1000 nM of human PTH (Sigma-Aldrich) and human AVP (SigmaAldrich) for 30 minutes at 37°C The cells were ... (p-values of < 0.05) unless stated otherwise 49 Results 4.1 Isolation of HPTC and characterization of freshly isolated and commercial HPTC 4.1.1 Isolation of HPTC HPTC are available commercially and...

Ngày tải lên: 09/09/2015, 10:19

... and mutant cells and extent of HIV Growth Growth rates of parental and mutant cells and extent of HIV integration (A) Growth rates Parental and mutant 30-2 and 42-7 cells were seeded and growth ... x 105 Figure cells with an HIV-EGFP vector at a moi = 0.5 Infection of parental and mutant HeLa cells (30-2 and 42-7) Infection of parental and mutant HeLa cells (30-2 and 42-7) cells with an ... fractionation of cytoplasmic and nuclear extracts and measurement of the amount of full product 36 hours after infection in parental (black bars) and 42-7 (grey bars) cells; (E) Parental and 42-7 cells...

Ngày tải lên: 13/08/2014, 05:22

... backbone amide and carbonyl of Cys140 form hydrogen bonds with the backbone carbonyl and amide of Trp123, the side chain of which forms one wall of the active site cavity and the replacement of which ... the uncatalyzed dismutation of superoxide [32] A least-square fit of Eqn (1) to the data of Fig yields the constants given in the legend of Fig The values of kcat ⁄ Km and kcat for catalysis by ... due to a loss of the interaction between the side chains of Asn73 and Gln143 The X-ray crystal structure of hMnSOD shows that the side chain amide ˚ of Asn73 is near to (3.1 A) and possibly hydrogen...

Ngày tải lên: 07/03/2014, 11:20

... Figure 23 Binding activity of 10.15 to various strains of DENV2 and DENV1, and 101   Figure 24 Binding activity of 12.17 to various strains of DENV2 and DENV1, and 102 ... Philippines and recent isolates from South Pacific islands Thailand, Vietnam and Bangladesh Sri Lanka, India, Africa, Samoa and 1962 strain from Thailand Puerto Rico, Latin and central America and 1965 ... CXCL10 and CXCR3 also exhibited lowered resistance to primary DENV infection as a result of faulty activation of CD8+ T cells and NK cells (Hsieh et al., 2006) On the other hand, CCR2 and CCR4...

Ngày tải lên: 09/09/2015, 08:16

... reaction 30 2.3.1 Preparation of competent E.coli cells A tube of glycerol stock of DH5-α Escherichia coli (E.coli) cells was thawed and 50 µL was used to innoculate mL of Lauria Bertiani (LB) medium ... from Brown and McNeilly, 1999) Unlike the mammalian FSH and LH, which are largely co-expressed in the same cells of the mature pituitary, teleost FSH and LH are produced in distinct cells, in ... stimulatory effects of GnRH and activin during puberty and sexual maturation 1.5 Aim The aim of this project is to initiate research on the mechanism of transcriptional regulation of the FSHβ subunit...

Ngày tải lên: 08/11/2015, 17:15

... disturb the loosely adherent cells, and ml per well of new AIM-V medium containing 560 u/ml of recombinant human GM-CSF (Research Diagnostic Inc., Flanders, NJ) and 25 ng/ml of IL-4 (R&D Systems, http://www.retrovirology.com/content/1/1/37 ... added and the cells were cultured at 37°C and 5% CO2 On Day 3, ml of fresh AIM-V medium containing 560 u/ml of GM-CSF and 25 ng/ml of IL-4 was added to the culture On Day 5, the non-adherent cells ... transduction of DCs and analysis of surface marker expression LV transduction of DCs and analysis of surface marker expression PBMC -derived DCs were infected with LV on Day (d5) and after maturation,...

Ngày tải lên: 13/08/2014, 13:20

... Aim and scope of the thesis Differentiation and lineage commitment of precursor cells are regulated by the expression and repression of a number of genes Preliminary transcriptome analysis of ... encompassing microvascular endothelial cells and even smooth muscle cells and a type of precursor cells called Adipose tissue derived cells (ADSCs) (Zuk PA et al., 2001) These cells have variably been known ... of stem cells and precursor cells for this purpose The two types of stem cells that are being targeted for use in tissue regeneration are the Embryonic stem cells and the Adult stem cells The...

Ngày tải lên: 08/11/2015, 17:01

... speed of the cells and analyze the behavior of the cells motility Figure 3.3 shows a screen print image of the interface of the IDL program The frames can be linked to form a continuous movie, and ... and constant support during this work I am also thankful to Shao and Jeroen for their guidance through the early years of chaos and confusion And the close cooperation from A/Prof Ge Ruowen and ... Vein Endothelial Cells (HUVEC, Figure 1.1 [4]) Figure 1.1: Micrograph of HUVE Cells plated on a flat substrate 1.2 Endothelial Cells and Angiogenesis What are Endothelial Cells and why we study...

Ngày tải lên: 26/11/2015, 12:55

... Import and assembly of proteins into mitochondria of mammalian cells Biochim Biophys Acta 1592, 97–105 19 Suzuki, H., Maeda, M & Mihara, K (2002) Characterization of rat TOM70 as a receptor of the ... respectively, and the resultant VNARs evaluated for retention of binding affinity Material and methods Equipment and reagents Restriction enzymes and Vent DNA polymerase were purchased from New England ... ampicillin and/ or 2% (w/v) glucose Solid media contained 2% (w/v) Bacto-agar Transformation of E coli was by standard procedures [24] performed using electrocompetent cells Isolation of total RNA...

Ngày tải lên: 17/03/2014, 10:20

... PBMC and tumor -derived GM-CSF, which supports expansion of myeloid progenitors and survival of MDSC, and IFNg, which contributes to MDSC activation Transactivation (*) between JAK/STAT, HIF1a, and ... CD11b+) showed an abundance of immature cells, including metamyelocytes or band cells and blast-like cells (middle left and far right panels, representative images shown of three independent experiments) ... Page 12 of 20 Figure Morphology, phenotype, and function of CD11b+ and CD33+ MDSC subsets A, Morphology of human CD33+ and CD11b+ MDSC subsets isolated after tumor cell line co-culture and normal...

Ngày tải lên: 18/06/2014, 19:20

... in the bottom of the tube (Fig 3D), and positive to toluidine blue staining (Figs 4E and F) Discussion Isolation and characterization of stem cells derived from various tissues and sources have ... condition C, E and G: Undifferentiation; D, F and H: Neuronal differentiation Nestin, Tuj-1 and NF160 were green GFAP and MAP2 were red Scale bars = 50 μm Isolation and characterization of canine ... Zhang X, Takahashi K, Mitsuru A, Yamaguchi S, Takashi TA Isolation and characterization of mesenchymal progenitor cells from chorionic villi of human placenta Cytotherapy 2004, 6, 543-553 15 Ishikawa...

Ngày tải lên: 07/08/2014, 23:22

... endothelial cells enhance osteogenesis and vascularization of mesenchymal stem cells Cells Tissues Organs 2009, 190:185-193 Rodbell M: The metabolism of isolated fat cells IV Regulation of release of ... potential of adult human mesenchymal stem cells Science 1999, 284:143-147 Pittenger MF, Mosca JD, McIntosh KR: Human mesenchymal stem cells: progenitor cells for cartilage, bone, fat and stroma ... efficient isolation and systematic identification of human adipose -derived mesenchymal stem cells Journal of Biomedical Science 2011 18:59 Submit your next manuscript to BioMed Central and take...

Ngày tải lên: 10/08/2014, 10:20

... may be derived from subsequent isolation and re-plating of the ICM cells, which are then screened for stemness by a variety of recognized markers Once in stable culture, ESCs are capable of either ... substantial ethical obstacle for the advancement of human ESC research With the vast therapeutic promise of human ESC seen against the destruction of human embryos required to realize such aspirations, ... support of and in opposition to human ESC research [3,4] It must be admitted that thus far human ESCs have provided no reproducible, safe, unique and previously unattainable treatment for any human...

Ngày tải lên: 13/08/2014, 23:20

... treatment of a type diabetes mouse model   xiv LIST OF FIGURES AND TABLES Figure 3.1 Schematic of human umbilical cord and the isolation of cord lining cells 51 Figure 3.2 Schematic of mixed leukocyte ... review will focus on the immunogenicity of various types of stem cells and progenitor cells Based on their time of isolation during ontogenesis, these cells can be loosely classified into three ... Fetal and neonatal stem cells come in between embryonic and adult stem cells in the developmental continuum Studies of these cells have provided information and insights into the biology of stem cells...

Ngày tải lên: 11/09/2015, 10:02

... study of isolation and characterization of a novel human gene was initiated The objectives of this study were as follow: To isolated full length cDNA; To analyze the genomic structure of MOST-1; ... information 167 vi LIST OF TABLES Types of virus-induced cancers 16 HPV gene products and their functions 18 List of cells with respective growth media used 28 List of primers and their respective ... cancers and prostate cancers, and is also the candidate site of potential oncogene(s) other than c-myc located at 8q24.1 Analysis of paired biopsies of invasive ductal breast cancer and adjacent...

Ngày tải lên: 16/09/2015, 17:13

... polysaccharide One milligram of the partially purified polymer contained 309.7 µg of protein, 160.2 µg of neutral sugar, 58.5 µg of hexosamine, 30.8 µg of uronic acid and 3.8 µg of phosphorus Constituent ... concentration of ppm to estimate the effect of pH After min, A660 was measured and the extent of flocculation was calculated Pronase digestion of extracellular polymer Forty milligrams of the extracellular ... as follows: 500 g of soil was washed times with distilled water and suspended in 1,000 ml of water The mixture was allowed to stand for 10min and the supernatant was collected and used for the...

Ngày tải lên: 05/09/2013, 09:08

... for cDNA single strand synthesis, 600 ng of RNA, 0.5 lg of oligo(dT) primer and 10 mm of dNTPs were incubated for at 65 °C Subsequently, lL of the first strand buffer X and lL of dithiothreitol ... III and P IV isoforms from the eluate P III ⁄ P IV (Fig 1C) Isoelectric focusing gave pI of 5.8, 5.7, 5.5 and 5.2 for the four isoforms respectively Secondary structure of the pulchellin isoforms ... mixture of other isoforms (B) Gel visualization of proteins eluted Lane 1, molecular weight markers Numbers on the left indicate the Mr values of the standards in thousands Lanes 2–9, lg of peak...

Ngày tải lên: 18/02/2014, 16:20

... activity of the human enzyme was not regained upon removal of EDTA and addition of metal-ions Figure shows the hydrophobicity profiles of human and P fluorescens ACMSD, performed according to Kyte and ... and biochemical characterization of human ACMSD Results Cloning of ACMSD transcripts The complete coding sequence of human ACMSD was obtained from reverse-transcribed human kidney Human ACMSD RNA, ... [21] From the sequence alignment of human and bacterial ACMSD and the comparison of their three-dimensional structures, the presence of the metallocofactor in the human enzyme can be reliably predicted...

Ngày tải lên: 19/02/2014, 02:20

... (2 lg) of QGP-1 cells using random hexamers and Superscript III (Invitrogen Corp.) One and two of 20 lL of the resulting single strand cDNA reaction were used as templates for RT-PCR and quantitative ... in exon of fragment C represents a deletion of 39 bp The thick lines indicate intron of 75 bp in fragments B and C, 3¢ portion of 35 bp in intron in fragments C and D, and 3¢ portion of 25 bp ... RNA of QGP-1 cells and pancreas (Fig 2A) Determination of the nucleotide sequences of the RT-PCR products revealed A Confirmation of utilization of exon 1a as a transcription initiation exon and...

Ngày tải lên: 19/02/2014, 06:20