derivation and characterization of human esc derived mesenchymal stem cells

... potential of adult human mesenchymal stem cells Science 1999, 284:143-147 Pittenger MF, Mosca JD, McIntosh KR: Human mesenchymal stem cells: progenitor cells for cartilage, bone, fat and stroma ... endothelial cells enhance osteogenesis and vascularization of mesenchymal stem cells Cells Tissues Organs 2009, 190:185-193 Rodbell M: The metabolism of isolated fat cells IV Regulation of release of ... isolation and systematic identification of human adipose -derived mesenchymal stem cells Journal of Biomedical Science 2011 18:59 Submit your next manuscript to BioMed Central and take full advantage of: ...

Ngày tải lên: 10/08/2014, 10:20

... differentiation of HUMSCs into cardiomyocytes and maturation of HUMSCs derived cardiomyocytes Admittedly, the detailed mechanism(s) of above effects of S1P on differentiation of and maturation of HUMSCs derived ... in CMCM and CMCM+S1P groups Figure 2A-C, G-I represents the fluorescent immunostaining of aactinin of cells from three groups, while, J-L shows the fluorescent immunostaining of MHC of cells from ... Review: Human Umbilical Cord Stroma with Regard to the Source of Fetus -Derived Stem Cells Stem cells 2007, 25:2886-2895 Wu KH, Mo XM, Zhou B, Lu SH, Yang SG, Liu YL, Han ZC: Cardiac potential of stem...

Ngày tải lên: 10/08/2014, 05:21

... Comparison of proliferative and multilineage differentiation potential of human mesenchymal stem cells derived from umbilical cord and bone marrow Stem Cells 2007, 25:1384-1392 Weiss ML, Anderson ... N, O’Valle F, Hernandez-Cortes P, Rico L, Büscher D, Delgado M: Human adipose -derived mesenchymal stem cells reduce inflammatory and T-cell responses and induce regulatory T cells in vitro in ... Age-related changes in human bone marrow -derived mesenchymal stem cells: consequences for cell therapies Mech Ageing Dev 2008, 129:163-173 Rao MS, Mattson MP: Stem cells and aging: expanding the possibilities...

Ngày tải lên: 12/08/2014, 15:22

... cells: MSCs, osteoprogenitor cells, osteoblasts, chondrocytes Mitogenic for mesenchymal cells and osteoblasts, chemotactic for inflammatory and mesenchymal cells Targeted cells: mesenchymal and ... inflammatory cells, osteoblasts Differentiation of undifferentiated mesenchymal cells into chondrocytes and osteoblasts and osteoprogenitor cells into osteoblasts Targeted cells: mesenchymal and osteoprogenitor ... chondrocytes maturation and bone resorption Targeted cells: mesenchymal and epithelial cells, osteoblasts and chondrocytes IGF-I: mesenchymal and osteoprogenitor cells recruitment and proliferation,...

Ngày tải lên: 11/09/2015, 09:57

... in the bottom of the tube (Fig 3D), and positive to toluidine blue staining (Figs 4E and F) Discussion Isolation and characterization of stem cells derived from various tissues and sources have ... increasing the passage number of adult stem cells often leads to a decline in the multipotent abilities [36] Human mesenchymal stem cells could be proliferated and have differentiation abilities ... Cotransplant of neural stem cells and NT-3 gene modified Schwann cells promote the recovery of transected spinal cord injury Spinal Cord 2007, 45, 15-24 12 Hipp J, Atala A Sources of stem cells for...

Ngày tải lên: 07/08/2014, 23:22

... Fanin E, Vettor R, Pagano C, Pandolfo M, Fabbri A: A real-time PCR approach to evaluate adipogenic potential of amniotic fluid -derived human mesenchymal stem cells Stem Cells Dev 2006, 15:719-726 ... HIV-1 and recombinant gp120 affect the survival and differentiation of human vessel wall -derived mesenchymal stem cells Retrovirology 2011 8:40 Submit your next manuscript to BioMed Central and ... infection of human bone marrow stromal fibroblasts Blood 1990, 76:317-322 40 Wang L, Mondal D, La Russa VF, Agrawal KC: Suppression of clonogenic potential of human bone marrow mesenchymal stem cells...

Ngày tải lên: 13/08/2014, 01:20

... backbone amide and carbonyl of Cys140 form hydrogen bonds with the backbone carbonyl and amide of Trp123, the side chain of which forms one wall of the active site cavity and the replacement of which ... the development of a selection system for improving hMnSOD activity based on the ability of Escherichia coli to grow in the presence of the toxic compound paraquat; and (b) the use of the paraquat-based ... the uncatalyzed dismutation of superoxide [32] A least-square fit of Eqn (1) to the data of Fig yields the constants given in the legend of Fig The values of kcat ⁄ Km and kcat for catalysis by...

Ngày tải lên: 07/03/2014, 11:20

... development of a cost effective production process for consistent supplies of HPLC-purified therapeutic human exosomes List of abbreviations MSC: Mesenchymal Stem cells; ESC: Embryonic stem cells; ... A: Role of stromal -derived factor-1 in the hematopoietic-supporting activity of human mesenchymal stem cells Eur J Haematol 2006, 76(6):488-493 Caplan AI, Dennis JE: Mesenchymal stem cells as ... cells to repair the heart: mesenchymal stem cells for the treatment of heart failure American journal of nephrology 2007, 27(3):301-307 Behfar A, Terzic A: Optimizing adult mesenchymal stem cells...

Ngày tải lên: 20/06/2014, 03:20

... concentration and annulus cells adiposederived mesenchymal stem of AD-MSCfor 3D co cultured alone Increase in separate culture cells (AD-MSC) and annulus cells comderived mesenchymal stem cells (AD-MSC) and ... localisation of the multipotent mesenchymal stem cell markers CD105 and CD29 and negative localisation of CD45 and CD34 [23] For cluster of differentiation (CD) immunohistochemical assessment of stem ... interactions of adult mesenchymal stem cells and disc cells by Le Visage et al [20] showed that annulus, but not nucleus, cells co-cultured in chondrogenic pellets with mesenchymal stem cells had...

Ngày tải lên: 09/08/2014, 10:23

... Page of 10 Figure Characterization of bone marrow -derived MSCs Flow cytometric analyses of bone marrow -derived MSCs Cells were uniformly negative for CD34, CD45, and positive for CD44, and CD29 ... Marshak DR: Multilineage potential of adult human mesenchymal stem cells Science 1999, 284:143-147 Dimmeler S, Leri A: Aging and disease as modifiers of efficacy of cell therapy Circ Res 2008, 102:1319-1330 ... of cell therapies Semin Thorac Cardiovasc Surg 2008, 20:94-101 Chen L, Tredget EE, Wu PY, Wu Y: Paracrine factors of mesenchymal stem cells recruit macrophages and endothelial lineage cells and...

Ngày tải lên: 10/08/2014, 05:21

... mechanism of action of MSCs, and KGF and angiopoietin-1 are Figure Bacterial Growth in vivo (A and B) and in vitro (C) Bacterial colony forming units in BAL fluid (A) and blood (B) at 1, 3, and days ... V, Lee JW, Matthay MA: Antibacterial effect of human mesenchymal stem cells is mediated in part from secretion of the antimicrobial peptide LL-37 Stem Cells 2010, 29:2229-2238 11 Rocha V, Wagner ... 11 of 11 34 Fang X, Neyrinck AP, Matthay MA, Lee JW: Allogeneic human mesenchymal stem cells restore epithelial protein permeability in cultured human alveolar type II cells by secretion of angiopoietin-1...

Ngày tải lên: 12/08/2014, 13:22

... and mutant cells and extent of HIV Growth Growth rates of parental and mutant cells and extent of HIV integration (A) Growth rates Parental and mutant 30-2 and 42-7 cells were seeded and growth ... x 105 Figure cells with an HIV-EGFP vector at a moi = 0.5 Infection of parental and mutant HeLa cells (30-2 and 42-7) Infection of parental and mutant HeLa cells (30-2 and 42-7) cells with an ... fractionation of cytoplasmic and nuclear extracts and measurement of the amount of full product 36 hours after infection in parental (black bars) and 42-7 (grey bars) cells; (E) Parental and 42-7 cells...

Ngày tải lên: 13/08/2014, 05:22

... Figure 23 Binding activity of 10.15 to various strains of DENV2 and DENV1, and 101   Figure 24 Binding activity of 12.17 to various strains of DENV2 and DENV1, and 102 ... Philippines and recent isolates from South Pacific islands Thailand, Vietnam and Bangladesh Sri Lanka, India, Africa, Samoa and 1962 strain from Thailand Puerto Rico, Latin and central America and 1965 ... CXCL10 and CXCR3 also exhibited lowered resistance to primary DENV infection as a result of faulty activation of CD8+ T cells and NK cells (Hsieh et al., 2006) On the other hand, CCR2 and CCR4...

Ngày tải lên: 09/09/2015, 08:16

... Isolation of HPTC and characterization of isolated and commercial HPTC 50 4.1.1 Isolation of HPTC 50 4.1.2 Characterization of HPTC by immunoblotting 51 4.1.3 Characterization of ... containing 0.1 mM of 3-isobutyl-1-methylxanthine (IBMX) (Merck) and exposure of cells to 100 nM and 1000 nM of human PTH (Sigma-Aldrich) and human AVP (SigmaAldrich) for 30 minutes at 37°C The cells were ... maintenance of renal epithelia 101 Discussion 105 5.1 Characterization of isolated and commercial HPTC 105 5.2 Characterization of effects of BMP-7 on HPTC and generation of a...

Ngày tải lên: 09/09/2015, 10:19

... Isolating Sternum -Derived Mesenchymal Stem Cells 46 3.1.1 Isolation of Bone Marrow Mesenchymal Stem Cells (MSCs) 46 3.1.2 Patient -derived cells can differentiate into osteoblasts and adipocytes ... 1.5.2 Adult stem cells 10 1.5.2.1 Bone marrow stem cells 10 1.5.2.1.1 Hematopoietic stem cells 11 1.5.2.1.2 Mesenchymal stem cells 11 1.5.3 Bone marrow stem cell-transplantation ... Shim Myocardial Matrix-driven Cardiac Differentiation and Integration of Human Mesenchymal Stem Cells [Manuscript submitted to Stem Cells and Development] Genevieve MY Tan, Jack WC Tan, Yee Jim...

Ngày tải lên: 12/09/2015, 09:42

... Chen B, et al Ex vivo expansion and in vivo infusion of bone marrow -derived Flk-1+CD31-CD34- mesenchymal stem cells: feasibility and safety from monkey to human Stem Cells Dev,2006,15(3):349-357 ... efficacy of stem cell transplantation, committed stem cells are isolated and purified, and single lineage stem cell transplantation is then performed Deng et al applied intravenous infusion of MSCs ... Arterial Perfusion of Marrow Stem Cells Chin J Integr Trad West Med 2004,24(11):999-1002 Yan Z, Hang D, Guo C, Chen Z Fate of mesenchymal stem cells transplanted to osteonecrosis of femoral head...

Ngày tải lên: 25/10/2012, 11:18

... neuron stem cells, bone marrow -derived mesenchymal stem cell, and peripheral blood -derived stem cells have been frequently investigated for their feasibility and safety in the treatment of stroke ... studies and, to a lesser extent, in humankind, the use of adipose -derived mesenchymal stem cells (ADMSCs) for the treatment of stroke has seldom been discussed [19] Compared with embryonic stems cells ... proliferation, and differentiation of stem cells [18,25] Recently, SDF-1α and its receptor CXCR4 are proven crucial in bone marrow retention of hematopoietic stem cells, angiogenesis, and recruitment of...

Ngày tải lên: 18/06/2014, 16:20

... As compared with embryonic stems cells and bone marrow -derived mesenchymal stem cells, adiposederived mesenchymal stem cells (ADMSCs) have the distinct advantages of being abundant, easy to obtain ... Ratajczak J, Ratajczak MZ: Trafficking of normal stem cells and metastasis of cancer stem cells involve similar mechanisms: pivotal role of the SDF-1-CXCR4 axis Stem Cells 2005, 23:879-894 Kee HJ, Sohn ... troponin I-positive myogenic-like cells (pink arrows) and undifferentiated adipose -derived mesenchymal stem cells (ADMSCs) (yellow arrows) in LV myocardium Merged image (F) of double staining [troponin-I...

Ngày tải lên: 18/06/2014, 16:20

... percentages of all EPC surface markers were similar between day and day 14 of cell culture Additionally, the percentages of MSC surface markers of CD45+ and CD271+ cells did not differ between day and ... IR damage Figure Impact of Adipose -Derived Mesenchymal Stem Cells (ADMSC) Transplantation on the Severity of IR-Induced Lung Parenchymal Injury Number of alveolar sacs and crowded area (was defined ... Therapeutic potential of mesenchymal stem cells for severe acute lung injury Chest 2010, 138:965-972 20 Chistiakov DA: Endogenous and exogenous stem cells: a role in lung repair and use in airway...

Ngày tải lên: 18/06/2014, 22:20