... referred
to as CPT1A, CPT1B, and CPT1C. Whereas CPT1A
is widely expressed in most tissues, CPT1B is only
expressed in muscle, adipose tissue, heart, and testis
[1], and CPT1C expression seems to be restricted ... occurring variation, Glu17, isresponsiblefor both the low malonyl-
CoA sensitivity and high carnitine affinity characteristics of the pig enzyme.
This is the first demonstration that a single ... part, for the high
degree of inhibition of the human enzyme.
Experimental procedures
Construction of deletions D18PigCPT1B and
D28PigCPT1B for CPT1B expression in P. pastoris
The deletions D18PigCPT1B...
... site and a sub-
strate-binding site for factor IX. Blood 74, 244–251.
10 Bouma BN & Griffin JH (1977) Human blood coagula-
tion factor XI. Purification, properties, and mechanism
of activation ... AA, Gilchrist GS & Owen WG (1996)
Genetic analysis and functional characterization of pro-
thrombins Corpus Christi (Arg382–Cys), Dhahran
(Arg271–His), and hypoprothrombinemia. Blood 88,
2611–2618.
30 ... FXI levelsand hemorrhagic episodes are usu-
ally associated with injury or surgery, but may be so
severe as to demand replacement therapy [16].
The genetic basis of this rare coagulation disorder...
... the
transcriptional factor that favors lysogeny and is
responsiblefor primary control of the lambda develop-
mental decision for lysis or lysogeny [4,7–9]. In the
absence of CIII, CII is rapidly ... a lack of interaction between r
32
and
CIII (Fig. 4). As for CII, competition between r
32
and
CIII for binding to HflB would also decide the extent
and efficiency of protection of r
32
by CIII, ... affinity
for r
32
–HflB interaction and CIII–HflB interaction
would play an important role in the inhibition of
proteolysis of r
32
by HflB.
Interestingly, both CII and r
32
are cytosolic sub-
strates for...
... synthesis, it might be involved in the stearic
acid incorporation after the de novo synthesis of this
lipid (i.e. the second hypothesis). According to this
hypothesis, previously raised for mammals ... t-test.
Acknowledgements
We thank Stephen Manon and Nadine Camougrand
(IBGC, Universite
´
de Bordeaux ⁄ CNRS) for helpful
discussions. This work was supported by the CNRS
and the Conseil Re
´
gional Aquitaine. Work at ... of
sn-2-acyl-1-lysoPI and 1 nmol of [
14
C]stearoyl-CoA. Incu-
bations were performed at 30 °C for 10 min. Reactions
were stopped by the addition of 2 mL of chloroform ⁄ meth-
anol (2 : 1, v ⁄ v) and 500 lL...
... site for
the hydroxylation reaction to occur. The same seems to hold
for the derivatives of mandelic acid: 3,4-dihydroxymandelic
acid is oxidized by tyrosinase, whereas 4-hydroxymandelic
acid is ... should be
poor substrates for tyrosinase. On the other hand, 3,4-
dihydroxybenzoic acid inhibited the dopachrome forma-
tion, although the exact mechanism of this reaction has not
been investigated ... reaction was assayed spectrophotometrically [13].
However, we have now shown that it does not result from
its interaction with the enzyme but from chemical reactions
in solution. What distinguishes...
... procedure.
Acknowledgements
We wish to thank Ingrid Duschanek and Elli Gerick
for excellent technical assistance, and Steen Malmmose
for assistance with growing plants. The Danish
National Research Foundation, the Danish ... containing
2% sucrose, and grown in 12 h dark ⁄ light cycles at 25 °C
and 20 l mol photonsÆm
)2
Æs
)1
and, under selective condi-
tions, 500 lgÆmL
)1
spectinomycin. For thylakoid isolation
and physiological ... nm
caused by photo-oxidation of P700 to P700
+
, followed
by a slower absorption decrease due to reduction of
P700
+
by Pc. The reaction between Pc and P700 is a
multistep reaction, which can be divided...
... side-chain mediating cyclization [18].
Substitution of this position with l-Cys1 (TL6) abol-
ished ligation and subsequent cyclization resulting in
complete hydrolysis. This observation indicates that
only ... the C-terminal amino acid is essential for recogni-
tion of the substrate and subsequent ligation and
macrocyclization. In addition, we speculated that
S-methyl-l-Cys4 is incorporated into the ... activity.
N-methylation induces conformational changes and
elevates rotational barriers [41]. This rigidification of
molecular dynamics gives rise to a preferential prefold
of the oligopeptide. The substitution...
... positioning of our client vis-à-vis competitors
Preparation of course materials:
Submission of course materials to client for approval
Psychometric Questionnaire:
Using Thomas International ... Capital Markets
and Risk
■ Risks in derivatives
■ Fixed income and swaps
■ Treasury risk management
■ Asset and liability
management
■ Credit derivatives
■ Asset securitisation
and CDOs
■ Commodity ... Training
Credit Analysis
■ Accounting concepts
and developments
■ Corporate credit analysis
■ Credit portfolio risk
management
■ Bank and country
risk analysis
■ Analysis of non-bank
financials
■...
... post-hydrolysis. Therefore, the dissoci-
ation of phosphateand ⁄ or ADP is likely to be responsiblefor resetting
of the transporter. The data indicate that, like ABCB1 and ABCC1,
the ‘power stroke’ for ... a conformation that contains
a low-affinity binding site for [
3
H]daunomycin imme-
diately post-nucleotide hydrolysis.
Discussion
A precise molecular mechanism for substrate translo-
cation by ... high-affinity
conformation for the transporter, which is supported
by the restoration of high-affinity binding in the ADP-
bound conformation. That this step of the catalytic
cycle is associated...
... mentioned b efore
except for the primer c oncentration (50 pmolÆ50 lL
)1
reac-
tion mix) and the concentration of the agarose slab gel used
to visualize the PCR products was 4% (2% Gibco-BRL and
2% ... 5¢ and 3¢ flanking
regions of each exon allowed us to identify 23 single nuc-
leotide polymorphisms and two i nsertion/deletion events in
the coding as well as the noncoding regions. A comparison ... 265/266-bp
fragment is split into two fragments (240 and 26 bp) for
each allele e xcept the M and F alleles, for wh ich no TaqIsite
has been created (Fig. 8, Step IB), because of mutations
(deletion o r...
... wavelengths. (A, excitation and emission at 340 nm
when Fura-2 is bound to calcium; B, excitation and emis-
sion at 380 nm when Fura-2 is free). Intracellular calcium
levels can then be calculated ... antibodies to SEP53 and the human SEP53
gene was cloned into a bacterial and insect cell-expres-
sion system for the purification and acquisition of
full-length protein for immunization, and to develop ... is that there is a relatively high degree of
cell and tissue specificity in HSP and related stress-acti-
vated transcription factor induction [39–44]. Consistent
with this, our initial analysis...