... carnitine: 1 mm (for standard comparison withother published data), and a concentration equal tothe Km for carnitine of each enzyme (for comparisonbetween mutants). In this article, we show ... observedbetween pig and human (Asp17 for human and Glu17 for pig) might be responsiblefor the kinetic charac-teristics of both CPT1B enzymes. Consequently, wegenerated two single mutant (pig E17D and humanD17E) ... concentrations(from 0 to 15 lm for P50H, H50P, P128H, H128P,PigE17DCPT1B and HumanD17ECPT1B, and from 0 to500 lm for D 18PigCPT1B and D28PigCPT1B). The assaywas performed at 1 mm carnitine as standard. To analyzePigE17DCPT1B...
... active conformation, Val371 forms contactswith neighboring residues that are important for stabi-lizing the active state (e.g. Asp189, whichis part of theS1 pocket responsiblefor the binding ... FXI levelsand hemorrhagic episodes are usu-ally associated with injury or surgery, but may be sosevere as to demand replacement therapy [16].The genetic basis of this rare coagulation disorder ... wild-type and mutant FXI cleavage by thrombin.Accordingly:Vt¼ V1Ãð1 À expðÀk à tÞÞwhere Vt and V¥are the velocities of S-2366 hydrolysis byformed FXIa at time t and ¥, respectively, and k is...
... factor that favors lysogeny and is responsiblefor primary control of the lambda develop-mental decision for lysis or lysogeny [4,7–9]. In theabsence of CIII, CII is rapidly degraded by the ... onproteolysis of the heat shock sigma factor r32, anothersubstrate of HflB. Like proteolysis of CII, r32proteol-ysis requires ATP. Both CIII and CIIIC inhibit thisproteolysis, with CIIIC ... ofproteolysis of r32by HflB.Interestingly, both CII and r32are cytosolic sub-strates for HflB. In addition, HflB also acts on severalmembrane-associated substrates, forwhich the mecha-nism of...
... synthesis, it might be involved in the stearicacid incorporation after the de novo synthesis of thislipid (i.e. the second hypothesis). According to thishypothesis, previously raised for mammals ... (PIS1 and PIS2) differing in theirsubstrate specificity in vitro: PIS1 prefers CDP-DAGspecies containing palmitic and oleic acids, whereasPIS2 prefers CDP-DAG species containing linoleic and linolenic ... t-test.AcknowledgementsWe thank Stephen Manon and Nadine Camougrand(IBGC, Universite´de Bordeaux ⁄ CNRS) for helpfuldiscussions. This work was supported by the CNRS and the Conseil Re´gional Aquitaine....
... procedure.AcknowledgementsWe wish to thank Ingrid Duschanek and Elli Gerick for excellent technical assistance, and Steen Malmmose for assistance with growing plants. The DanishNational Research Foundation, the Danish ... plants; one for assembly of the PSI core complex and the other for integrity and stabilization of a luminal domain invol-ving at least PSI-N and the N-terminal part of PSI-F which is required for efficient ... 20 l mol photonsÆm)2Æs)1 and, under selective condi-tions, 500 lgÆmL)1spectinomycin. For thylakoid isolation and physiological measurements, wild-type and transformedplants (originating...
... (Ecm17) is found within the biosynthetic operon proposed to be responsible for disulfide formation [35]. This oxidore-ductase, although lacking in the gene cluster enablingthiocoraline biosynthesis, ... pharmaceuticalproperties.ResultsExpression and isolation of TioS T-TE as activeapo-form proteinTioS T-TE was heterologously expressed in Escherichiacoli M15 ⁄ pREP4 cells at 20 °C and isolated as a C-ter-minally His6-tagged apo-form ... hydrolysis was detected. Thisresult indicated that the steric demand or the polarityof the C-terminal amino acid is essential for recogni-tion of the substrate and subsequent ligation and macrocyclization....
... prior to hydrolysis. The low-affinity state wasobserved to be retained immediately post-hydrolysis. Therefore, the dissoci-ation of phosphate and ⁄ or ADP is likely to be responsiblefor resettingof ... high-affinityconformation for the transporter, whichis supportedby the restoration of high-affinity binding in the ADP-bound conformation. That this step of the catalyticcycle is associated ... electron microscopy [12]. Thisreport indicated that soluble purified ABCG2 dis-played a propensity to form a higher order oligomer, atetramer of dimers, whichis consistent with the obser-vations...
... exon 6 and exon 8 ofalleles F and M, whichis lacking in the A allele, giving atotal of 25 polymorphic sites useful for phylogenetic allelecomparisons. Taking into account these data, it is worthnoting ... indicatedpolymorphic a mino-acid residues and their position in t he p eptide chain. A sim plified haplotype formula is thus provided (e.g. HPSPERT and HLSPQRT for alleles B2 and A , r espect ively) . The ... Grosclaude and Martin [6] for theas1-Cas alleles and differences between the corresponding variants. Thephylogenetic t ree proposed is based on the existence of a singleancestra l allele ( B1), which...
... wavelengths. (A, excitation and emission at 340 nmwhen Fura-2 is bound to calcium; B, excitation and emis-sion at 380 nm when Fura-2 is free). Intracellular calcium levels can then be calculated ... is that there is a relatively high degree ofcell and tissue specificity in HSP and related stress-acti-vated transcription factor induction [39–44]. Consistentwith this, our initial analysis ... cells(Fig. 3E) andis consistent with data published recentlyin a different oeopshageal cancer cell line [25].DCA-mediated apoptosis is mediated by aPKC-dependent pathway andis p53 independentOne...