hormone responsible for increasing glucose uptake by cells
Tài liệu Báo cáo khoa học: Is ATP binding responsible for initiating drug translocation by the multidrug transporter ABCG2? docx
... post-hydrolysis. Therefore, the dissoci-ation of phosphate and ⁄ or ADP is likely to be responsible for resettingof the transporter. The data indicate that, like ABCB1 and ABCC1,the ‘power stroke’ for translocation ... 108plaque-forming unitsÆmL)1) at a multiplicity ofinfection of five. After 1 h of incubation with virus, the cells were diluted to a density of 1.5 · 106 cells mL)1andmaintained in suspension for ... conformation that containsa low-affinity binding site for [3H]daunomycin imme-diately post-nucleotide hydrolysis.DiscussionA precise molecular mechanism for substrate translo-cation by...
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Báo cáo khoa học: Identification of the structural determinant responsible for the phosphorylation of G-protein activated potassium channel 1 by cAMP-dependent protein kinase pdf
... determinant responsible for PKAphosphorylation in vitroTo identify the structural determinants that are respon-sible for phosphorylation of GIRK1 by PKA, fusionproteins comprising truncated forms ... eliminate the effects of cAMP by mutatingS385, S401 and T407 may be that isoforms other thanGIRK1 may be also targets for protein phosphoryla-tion in the protein complex: for example, GIRK2 hadbeen ... oocyte). After incubation of cells for 6 days at 19 °C,the oocytes were checked for expression with the two elec-trode voltage clamp technique as described below and the cells were injected with...
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Báo cáo khoa học: Connection of transport and sensing by UhpC, the sensor for external glucose-6-phosphate in Escherichia coli ppt
... encodes the mutated UhpC-H222Q protein.The cells were induced for 15 min with given Glc6P concentrations. For quantification of uptake cells were incubated for 1 min with 10 lM[14C]Glc6P. Inset: ... coli cells BL21(DE3) (A) and E. coli cells BL21(DE3) (uhpC::Tn1000) harbouring plasmid uhpC/pET16b (B) were induced for 15minwithgivenGlc6P concentrations. For quantification of uptake, cells ... increased affinity for sensing. Taken together, thesedata show that transport by UhpC is not required for sensing, that conserved arginine residues are important for sensing and not for transport,...
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Báo cáo y học: "High-Resolution Flow Cytometry: a Suitable Tool for Monitoring Aneuploid Prostate Cancer Cells after TMZ and TMZ-BioShuttle Treatment"
... to kill cells regard-less of intrinsic cellular sensitivity and cell DNA-index. Therefore, we reformulated the TMZ by ligation to a peptide-based carrier system called TMZ-BioShuttle for intervention. ... 4-diamidino-2-phenylindole (DAPI) for staining the cell suspension was performed. Each histogram represents 30.000 cells for measuring DNA-index and cell cycle. For histogram analysis we used ... CO2 for 144 hours. Treatment of lymphocytes was followed by identical procedures according to the DU 145 cells. The suspension culture was harvested by centrifuga-tion at 800rpm for 10min,...
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Flavor enhancement as a tool for increasing pleasantness and intakeof a snack product among the elderly
... given differentinformation on the samples for the home-use. One group inboth age cohorts was informed about the organic quality ofthe sample and another group received information aboutthe ... presence of health-related information. Since the effectsof information were negligible, and not relevant enough for this study, the data were pooled together over theinformation conditions.Olfactory ... mean scores(SEM, range) for the young subjects were 15.8 (0.18, 14–16) for detection and 13.5 (0.30, 9–16) for identification ofa possible 16. The corresponding scores for the elderly were14.7...
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PHP/MySQL Programming for the Absolute Beginner by Andy Harris
... this book). information. Building an HTML Page with a Form Forms are very useful when you want to get information from the user. To illustrate how this is done, look at the code for the whatsName.html ... information into a plain HTML form and hits the submit button. The PHP program doesn't execute until after the user has submitted a form. The program takes the information from the form ... text styles. You might want to have one kind of paragraph for quotes, for example, and another type for ordinary text. To use the special form of the tag, just use the class attribute in the...
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Cost Allocation for Joint Products and By-Products
... Inventory for July? What amount of cost for towels is transferred to Finished GoodsInventory for July?42. (Joint products; by- product) Valley Mangoes runs a fruit-packing business insouthern California. ... added to company income by managing the costs andrevenues related to the by- products/scrap. The entries for the processing and saleof the by- products/scrap under this method for the Ballad Beef ... ac-counting for by- product income for the Ballad Beef Company. Some assumedamounts have been included to provide complete income statements.Chapter 9 Cost Allocation for Joint Products and By- Products359(a)Net...
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Tài liệu Investigation of Organic Chemicals Potentially Responsible for Mortality and Intersex in Fish of the North Fork of the Shenandoah River, Virginia, during Spring of 2007 ppt
... described by Alvarez and others (in press).HormonesFour common natural and synthetic hormones were targeted in this study. Extracts selected for hormone analy-sis required derivatization of the hormones ... individual samples designated for processing for agricultural pesticides, hormones, pharmaceuticals, and the YES assay.The procedures used for preparing SPMD samples for analysis were similar ... measured by polar organic chemical integrative samplers (POCIS) in the North Fork of the Shenandoah River, Virginia 15 6. Estimated water concentrations of select hormones measured by polar...
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Tài liệu Báo cáo khoa học: Identification of two late acyltransferase genes responsible for lipid A biosynthesis in Moraxella catarrhalis doc
... except for UDP-GlcNAc acyltransferase, responsible for lipid A biosynthesis inM. catarrhalis, have been identified. By bioinformatics, two late acyltrans-ferase genes, lpxX and lpxL, responsible for ... identified by digestion with BamHI andSacI as well as by sequence analysis, and named pWlpxXand pWlpxL. pWlpxX and pWlpxL were used to transformO35ElpxX and O35ElpxL competent cells by electropora-tion, ... used for electroporation to O35E competent cells [19].The resulting Kanrcolonies were selected for PCR analysisusing primers b2E and b2B, and the inactivated lpxLmutant was verified by sequencing.Southern...
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Tài liệu Báo cáo khoa học: Functional studies of active-site mutants from Drosophila melanogaster deoxyribonucleoside kinase Investigations of the putative catalytic glutamate–arginine pair and of residues responsible for substrate specificity docx
... analmost 2000-fold decrease in the kcatvalue for dThd,and a 270-fold decrease for dCyd. The Kmvalue wasincreased sevenfold for dThd and 49-fold for dCyd.The large decrease in kcatvalue ... BVDU. Km for AraC wasincreased 59-fold, but this was less pronounced thanthe increase of 106-fold for dCyd. Vmaxwas fairlyunchanged compared with the wild-type. The Kmvalue for BVDU was ... was determined by SDS ⁄ PAGE [20] and the protein concentrations were deter-mined using Bradford reagent [21].Enzyme assaysDeoxynucleoside kinase activities were determined by initialvelocity...
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