hongxia master of science chemistry chitin hydrolysis sulfation and molecular weight analysis 2006 d pdf

Construction of bacterial artificial chromosome library for kineosphaera limosa strain lpha5t and screening of genes involved in polyhydroxyalkanoate synthesis

Ngày tải lên : 03/10/2015, 21:58

... studied in detail with Pseudomonas spp and Ralstonia spp (Anderson & Dawes, 1990) However, PHA metabolism in bacteria involved in the EBPR processes is little understood, and suspected to be different ... acetate and propionate are taken up and stored as reserved materials such as polyhydroxyalkanoates (PHAs) This is accompanied by the degradation of internal polyP and glycogen and the release of Pi ... precipitates are removed at different stages based on the adding point of salts However, the cost of chemical treatment is high, and the amount of daily wasted sludge is also largely increased Chapter From...

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The roles of DNA(cytosine 5) methyltransferase1 in carcinogenesis related to cellular factors, virus and chemicals

Ngày tải lên : 16/09/2015, 15:55

... could be demethylated by depleting DNMT1 in the CaSki cells, using 5AzadC and MMS This led to the induction of the late genes, and an inhibition of the early genes A phenomenon of TSA induced repression ... pairs of cyclins/cdks; including cyclin D/ cdk4, cyclinE/cdk2, cyclinA/cdk2 and cyclinB/cdc2 that control the entry points of G1, S, G2 and the G2/M boundary of the cell cycle (Kelman and Hurwitz, ... remodelling and histone deacetylase NuRD to methylated DNA in vitro (Feng and Zhang, 2001) Evidence for the mechanistic link between DNA methylation and histone deacetylation was demonstrated by...

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Báo cáo y học: " Helicobacter pylori induces mitochondrial DNA mutation and reactive oxygen species level in AGS cells"

Ngày tải lên : 25/10/2012, 11:18

... demonstrated the ROS levels and the frequency of mutations in the mtDNA increased and the cell viability decreased in a concentration and time dependent manner In addition, the ROS levels and ... finally mixed and freeze-dried The dry powder was dissolved in ml of sterile double-distilled water and stored at -40℃ for use Immediately before use, the solution was centri- http://www.medsci.org ... Promega Co USA), Dihydrorhdamine-123 (DHR-123) and oxidized cytochrome c (Sigma, USA) and AGS mtDNA extraction kit GenMed Scientifics Co., Ltd USA) were used in the present study Cells and Hp Culture...

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Báo cáo y học: "NITRIC OXIDE (NO), CITRULLINE – NO CYCLE ENZYMES, GLUTAMINE SYNTHETASE AND OXIDATIVE STRESS IN ANOXIA (HYPOBARIC HYPOXIA) AND REPERFUSION IN RAT BRAIN"

Ngày tải lên : 26/10/2012, 09:07

... quickly removed and the different regions (CC, CB and BS) were separated according to the procedure described by Sadasivudu and Lajtha (22) Each of the brain regions was weighed and used for the ... animals from desiccator, they were allowed to stay at normal conditions and were given normal diet for days and decapitated and the different brain regions (CC, CB and BS) were used for study It was ... Int J Med Sci 2010, leased from damaged axons and glia under hypoxic/ischemic conditions (4) and glutamate receptor-mediated excitotoxicity has been described as a predominant mechanism of hypoxic...

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Tài liệu .An ARCO Book ARCO is a registered trademark of Thomson Learning, Inc., and is used herein under pptx

Ngày tải lên : 20/01/2014, 20:20

... governments view software piracy as an industry in and of itself and look the other way U.S firms stand to lose millions of dollars in new business, and diminished U.S sales not only harm individual firms ... is a standardized exam given at various locations in the United States and Canada and around the world Throughout North America and in many international locations, the GMAT is administered only ... Thus, the CAT is “adaptive” or “interactive.” Whereas candidates taking the old paper-based test were presented with a range of questions (including easy, moderately difficult, and difficult items),...

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Báo cáo khoa học: Hyperthermophilic enzymes ) stability, activity and implementation strategies for high temperature applications pot

Ngày tải lên : 07/03/2014, 05:20

... sorbent and charged and ⁄ or hydrophobic amino acid side chains Depending on the adsorbing conditions, as well as the protein and surface properties, these interactions, which individually are ... residual activity: ADH-only and ADH-chaperone yielded residual activities of 15% and 78%, respectively, after days [81] 4050 Crucial for the development and optimization of hightemperature biocatalysis ... MCL, Miranda MCS & Barbosa MA (2005) Adsorption of a therapeutic enzyme to selfassembled monolayers: effect of surface chemistry and solution pH on the amount and activity of adsorbed enzyme...

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Báo cáo khoa học: "Case markers and Morphology: Addressing the crux of the ﬂuency problem in English-Hindi SMT" pot

Ngày tải lên : 08/03/2014, 00:20

... MacCartney, B., and Manning, C., Generating Typed Dependency Parses from Phrase Structure Parses, Proceedings of LREC, 2006 Marie-Catherine de Marneffe and Manning, C., Stanford Typed Dependency Manual, ... objects of prepositions, and clausal complements, modifier relations like adjectival, adverbial, participial, and infinitival modifiers, and other relations like coordination, conjunct, expletive, and ... relations produced by the Stanford parser The relations in both UNL and the Stanford dependency parser are strictly binary and form a directed graph These relations express the semantic dependencies...

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DNA Replication and Related Cellular Processes Edited by Jelena Kušić - Tišma pdf

Ngày tải lên : 08/03/2014, 19:20

... show DNA staining by DAPI (A and A’), EdU staining (B and B’) and merged images (C and C’) Note that cells that completed S phase prior to EdU addition stain with DAPI but not incorporate EdU, ... Nterminus and are degraded by CRL4Cdt2 As PCNA is an auxiliary factor of DNA polymerases, the degradation of CDT-1 and CKI-1 can be coupled to DNA replication Inactivation of CKI-1 allows activation of ... propidium iodide staining will probably be applied more broadly in the analysis of the DNA distribution of specific tissues and cells of interest Fig EdU incorporation and staining visualizes DNA...

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Lecture 3 DNA RNA and protein synthesis great

Ngày tải lên : 13/03/2014, 16:36

... Components of genes,  Director of protein synthesis   AND- DNA is also A type of nucleic acid  A type of organic compound  A polymer {a compound made of repeating subunits}   WHAT DOES “DNA” STAND ... single-stranded and acts as a code for protein synthesis  RNA is still made of nucleotides that have a phosphate, a sugar, and a nitrogen base  The sugar is different and the basepairing is also different ... of DNA A nucleotide of DNA is the base unit  A nucleotide consists of a phosphate, a sugar, and a nitrogen base  DNA is in a double strand  The nitrogen bases have compliment partners  Adenine-Thymine...

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DNA, RNA and proteins

Ngày tải lên : 13/03/2014, 16:38

... stranded, not double stranded b) Instead of deoxyribose, it has ribose c) instead of Thymine, it uses Uracil 3) Carries the genetic code from the nucleus to the ribosome in the cytoplasm DNA and ... cytoplasm DNA and Protein Synthesis – Codons and amino acids Name the Amino Acid: UAU Tyrosine CCG Proline AGU Serine GCA Alanine DNA and Protein Synthesis But… How does the information get from the DNA ... Proteins everything, and DNA gets all the credit! • Think of them as tiny minions who all the work DNA and Protein Synthesis DNA and Protein Synthesis • Codon: three bases code for a specific protein:...

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Báo cáo khoa học: Modeling hydration mechanisms of enzymes in nonpolar and polar organic solvents potx

Ngày tải lên : 16/03/2014, 10:20

... system This study has provided a detailed molecular picture of the hydration mechanism of an enzyme, and shown it to be clearly dependent on the nature of the organic solvent and water content ... replicate of cutinase hydrated with a speciﬁc amount of water was placed in a dodecahedral box with a minimum distance between the protein and box wall of 0.8 nm, and solvated with an equilibrated conﬁguration ... Acetonitrile revisited: a molecular dynamics study of the liquid phase Mol Phys 104, 477–483 53 Lide DR (2003) CRC Handbook of Chemistry and Physics, 84th edn CRC Press, London 54 Longhi S, Czjzek...

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Biomass for renewable energy, fuels and chemicals

Ngày tải lên : 16/03/2014, 18:10

... forbs, and shrubs suitable for grazing and browsing Other land areas include crop and pasture land and farmsteads, strip mines, permanent ice and snow, and land that does not fit any other land cover ... energy-containing forms of carbon and includes all land- and water-based vegetation and such materials as municipal solid wastes, forestry and agricultural residues, municipal biosolids, and some industrial ... Water Inland water Other water Total Grand total land and water 24.75 19.28 44.03 956.54 2.6 2.0 4.6 aAdapted from USDA Forest Service (1989) The data for forest, rangeland, and other land are for...

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Báo cáo khoa học: Alizarine derivatives as new dual inhibitors of the HIV-1 reverse transcriptase-associated DNA polymerase and RNase H activities effective also on the RNase H activity of non-nucleoside resistant reverse transcriptases pot

Ngày tải lên : 22/03/2014, 16:20

... molecular modeling results 3D models of docking and MD simulation results for visualization were created using the VMD and ligandscout software [49] Acknowledgements This work was supported by Fondazione ... reduction of efavirenz binding and, vice versa, the binding of efavirenz leads to a reduction of the AQ binding Docking studies These results demonstrated that the AQs and NNRTI binding sites ... whether the AQ derivatives could also bind to this site For this purpose, and to obtain a deeper understanding of the RTligand interactions, QM polarized docking (Schrodinger Inc, Portland, OR, ă USA)...

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Báo cáo khoa học: K182G substitution in DevR or C8G mutation in the Dev box impairs protein–DNA interaction and abrogates DevR-mediated gene induction in Mycobacterium tuberculosis doc

Ngày tải lên : 22/03/2014, 16:20

... Phosphorylation of WT and K182G mutant DevR proteins with DevS201P (phosphorylated cytoplasmic domain of DevS) Lane 1, DevSP; lane 2, DevSP and DevR K182G mutant protein; and lane 3, DevSP and WT DevR ... initiation of translation of FdxA 2136 In conclusion, the results of mutational analysis of protein and DNA establish the singular importance of G13–Lys182 H-bonding in DevR–DNA interaction and for downstream ... footprinting of fdxA promoter DNase I footprinting assays were performed with phosphorylated WT DevR protein and fdxA promoter DNA variants (WT and mutant) or K182G mutant DevR protein and WT fdxA promoter...

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Báo cáo khoa học: Nuclear aggregates of polyamines are supramolecular structures that play a crucial role in genomic DNA protection and conformation potx

Ngày tải lên : 23/03/2014, 15:20

... c and c¢), medium-size NAP (m-NAP; lanes d and d ) or largesize NAP (l-NAP; lanes e and e¢) and exposed to DNase I Controls were the whole genomic DNA (lane a) and the DNA fully digested by DNase ... preincubated with three different concentrations (1, 50 and 150 lM) of spermine (lanes c, c¢ and c¢¢), spermidine (lanes d, d and d ¢) or putrescine (lanes e, e¢ and e¢¢) and then exposed to DNase ... peripheral blood leukocytes donated by M di Pietro DNA was extracted and puriﬁed in phenol ⁄ chloroform and then resuspended in Tris ⁄ EDTA (TE) buffer NAPs were extracted from the nuclei of preconﬂuent...

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Methylation - From DNA, RNA and Histones to Diseases and Treatment by Anica Dricu ppt

Ngày tải lên : 24/03/2014, 02:21

... palindromic sequences (2–6 bp) in dou‐ ble-stranded DNA One or both DNA strands can be methylated The introduced methyl group is localized in the major groove of the DNA double helix and thus does ... unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited 4 Methylation - From DNA, RNA and Histones to Diseases and Treatment From DNA, RNA and Histones ... unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited 30 Methylation - From DNA, RNA and Histones to Diseases and Treatment From DNA, RNA and Histones...

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Báo cáo khoa học: DNA binding and partial nucleoid localization of the chloroplast stromal enzyme ferredoxin:sulﬁte reductase pptx

Ngày tải lên : 30/03/2014, 08:20

... isolated nucleoids [26] Radiolabeled DNA and poly(dI-dC)Æpoly(dI-dC) competed comparably for binding to SiR, and all the restriction fragments of both k and chloroplast DNA were shifted by SiR binding ... (C, D, G, H) and AlexaFluor488-tagged secondary antibodies, and stained with 4¢,6-diamidino-2-phenylindole Left-hand panels (A, C, E, G) display 4¢,6-diamidino-2-phenylindole and chlorophyll ... 17 and 12%, respectively, of that without a competitor, indicating comparable afﬁnities of SiR for the 20-mer dsDNA and the poly(dI-dC) The k DNA digested with StyI and pea chloroplast DNA digested...

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Báo cáo khoa học: Constitutive expression of the human peroxiredoxin V gene contributes to protection of the genome from oxidative DNA lesions and to suppression of transcription of noncoding DNA pdf

Ngày tải lên : 30/03/2014, 11:20

... menadione-induced DNA oxidation was also found when human recombinant full L-PRDX5 (Long-PRDX5) was added to the medium [Dulbecco’s modiﬁed Eagle’s medium (DMEM) without serum and phenol red] ... spontaneous DNA oxidation and DNA oxidation induced by CSE, and can suppress menadione-induced cell death, making a small contribution to protection from oxidation of nuclear DNA induced by menadione ... mice [16] and in transfected human tendon cells [17] Recent data indicate that this protein suppresses accumulation of etoposide-induced DNA double-strand breaks and may be involved in the global...

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Báo cáo khoa học: "Local constraints on sentence markers and focus in Somali" doc

Ngày tải lên : 31/03/2014, 01:20

... Ramsay and R Sch¨ ler 1997 Case and word ora der in English and German In R Mitkov and N Nicolo, editors, Recent Advances in Natural Language Processing John Benjamin A M Ramsay, Najmeh Ahmed, and ... 1(6):123– 191 D R Dowty, R E Wall, and S Peters 1981 Introduction to Montague Semantics D Reidel, Dordrecht Killian Foth, Wolfgang Menzel, and Ingo Schr¨ der o 2005 Robust parsing with weighted constraints ... Louisa Sadler 1996 New developments in LFG In Keith Brown and Jim Miller, editors, Concise Encyclopedia of Syntactic Theories Elsevier Science, Oxford J I Saeed 1984 The Syntax of Focus and Topic...

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Báo cáo khoa học: DNA-binding and transcription characteristics of three cloned sigma factors from mustard (Sinapis alba L.) suggest overlapping and distinct roles in plastid gene expression doc

Ngày tải lên : 31/03/2014, 07:20

... apparent, and this effect was more pronounced in the presence of SASIG1 and SASIG2 compared to SASIG3 and r70 This was most evident in the case of M-19 A/G (Fig 6B, third row, middle panel) and M-22 ... bp downstream of the transcription start site) was generated Plasmid pBS-1.7kb-B, including the promoter and both coding and noncoding regions of the ycf3 gene (AJ242660), was cleaved with DdeI ... electrophoretically separated, immunoblotted, and probed with anti-SASIG2 and anti-SASIG3 sera As shown in Fig 2A, each antiserum detected one single band, at 61 kDa (SASIG2) and 65 kDa (SASIG3), respectively,...

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