tổng quan điều kiện tự nhiên và kế hoạch phát triển của thành phố lào cai tỉnh lào cai

Methods in molecular biology vol 1537 oral biology molecular techniques and applications 2nd edition

Ngày tải lên : 25/12/2016, 15:08

... Preparation Kit by using 700 μL saliva aliquots per isolation DNA quantification using PicoGreen (a) Measure DNA quantity using the Quant-iT™ PicoGreen® dsDNA Assay Kit (see Note 7) ●● ●● ●● ●● Prepare ... Biosciences [46] Isolated exosomes were quantitated by a cholesteryl ester transfer protein (CETP) assay (EXOCET, System Biosciences) validated for the purification and quantification of exosomes [47, ... Kit Quant-iT™ PicoGreen® dsDNA Assay Kit (Life Technologies) 3 Methods Recently, the Pure⋅SAL™ device has been compared to whole saliva and validated for the collection of exosomes [45], quantified...

499
3.8K
0

Investigations on the roles of ubiquitin in the regulation of heat shock gene HSP70B 2

Ngày tải lên : 10/09/2015, 08:27

... 2.4.4 Stripping Western Blots 76 2.4.5 Split-ubiquitin Screens 76 2.5 Tools for mRNA Extraction and Quantification 78 2.5.1 Transfection of siRNA constructs and Treatments Prior to mRNA Isolation ... 2.5.2 Isolation of mRNA 78 2.5.3 DNAaseI Treatment and Reverse Transcription 80 2.5.4 Real-Time Quantative PCR 81 2.6 Chromatin Immunoprecipitation 83 v Chapter 3: Results 86 3.1 Introduction ... and Protein G Sepharose Beads 89 Figure 4: Knockdown of Skp1 by a pSuper construct 90 Figure 4.1: Quantification of “Knockdown of Skp1 by a pSuper construct” 91 Figure 5: Measurement of HSP90 and...

16
266
0

Báo cáo khoa học: Nuclear actin and actin-binding proteins in the regulation of transcription and gene expression docx

Ngày tải lên : 07/03/2014, 01:20

17
573
0

báo cáo khoa học: " Characterization of Vitis vinifera NPR1 homologs involved in the regulation of Pathogenesis-Related gene expression" ppsx

Ngày tải lên : 12/08/2014, 03:20

... Boostrap values were obtained from 500 replicates Gene expression analysis by semi- quantitative PCR and real-time quantitative PCR RNA extraction and DNase I treatment were performed as described ... http://www.biomedcentral.com/1471-2229/9/54 Transcript accumulation of VvNPR1.1 and VvNPR1.2 was then quantified after P viticola infection As shown in Figure 3B and 3C, no significant change in the ... amplification was revealed when omitting the reverse transcription step (data not shown) Real time quantitative PCR was used to study the expression of VvPR1 and VvSTS in grapevine leaves expressing...

14
303
0

NOVEL MECHANISMS IN THE REGULATION OF PERINUCLEAR ACTIN ASSEMBLY 2

Ngày tải lên : 09/09/2015, 08:16

... mDia2 I showed that this formin localized to the cytoplasmic side of nuclear membrane Further, quantitative measurement using fluorescence correlation spectroscopy (FCS) revealed reduced motility...

141
266
0

Investigations on the roles of ubiquitin in the regulation of heat shock gene HSP70B 1

Ngày tải lên : 10/09/2015, 08:27

3
299
0

Investigations on the roles of ubiquitin in the regulation of heat shock gene HSP70B 3

Ngày tải lên : 10/09/2015, 08:28

198
509
0

Development of cell sheet constructs for layer by layer tissue engineering using the blood vessel as an experimental model 2

Ngày tải lên : 14/09/2015, 08:49

3
198
0

An investigation of the information content of the financial policies of chinas listed companies 2

Ngày tải lên : 16/09/2015, 08:29

... Changes, Journal of Financial and Quantitative Analysis 19(2): 197–216 180 Dierkens, N (1991), Information Asymmetry and Equity Issues, Journal of Financial and Quantitative Analysis 26(2): 181-199 ... Investment Opportunities and the Market Reaction to Equity offerings, Journal of Financial and Quantitative Analysis 29 (2): 159-177 Denis, D J., Denis, D K., and Sarin, A (1994), The Information ... Changes: Cash Flow Signaling, Overinvestment, and Dividend Clienteles, Journal of Financial and Quantitative Analysis 29: 567-87 Dhatt, Kim and Mukerji (1994), Japanese Stock Price Reactions to...

57
423
0

$Tài liệu Characterization of the Polymorphic Behavior of an Organic Compound Using a Dynamic Thermal and X-ray Powder Diffraction Technique pptx$

Tài liệu Characterization of the Polymorphic Behavior of an Organic Compound Using a Dynamic Thermal and X-ray Powder Diffraction Technique pptx

Ngày tải lên : 14/02/2014, 03:20

... 257–259 (17) Rocco, W L.; Morphet, C.; Laughlin, S M Int J Pharm 1995, 122, 17–25 (18) Chang, L.-C.; Caira, M R.; Guillory, J K J Pharm Sci 1995, 84, 1169–1179 (19) Ghodbane, S.; McCauley, J A Int ... Brittain, H G Spectroscopy 2001, 16, 14–16–18 (24) Fawcett, T G.; Martin, E J.; Crowder, C E.; Kincaid, P J.; Strandjord, A J.; Blazy, J A.; Armentrout, D N.; Newman, R A AdV X-Ray Anal 1986, 29, ... degradation immediately follows the melt and creates an uncertain baseline Second, the varying quantities of water in the starting material (Form I) result Table Summary of DSC data, suggested...

16
549
0

Tài liệu Báo cáo khoa học: Understanding the binding properties of an unusual metal-binding protein ) a study of bacterial frataxin pdf

Ngày tải lên : 18/02/2014, 16:20

... with which to explore ion binding We ﬁrst revisited in a systematic way the effects of increasing quantities of iron (Fe2+ and Fe3+) on the [1H,15N]-HSQC NMR spectrum of CyaY, and then compared...

12
704
0

Tài liệu Báo cáo khoa học: Coordination chemistry of iron(III)±porphyrin±antibody complexes In¯uence on the peroxidase activity of the axial coordination of an imidazole on the iron atom ppt

Ngày tải lên : 21/02/2014, 03:20

11
470
0

Báo cáo khoa học: The catalytic mechanism of dye-decolorizing peroxidase DyP may require the swinging movement of an aspartic acid residue potx

Ngày tải lên : 06/03/2014, 00:20

8
392
0

Báo cáo khoa học: Regulation of Cyr61/CCN1 gene expression through RhoA GTPase and p38MAPK signaling pathways Role of CREB and AP-1 transcription factors doc

Ngày tải lên : 08/03/2014, 08:20

... with a GAPDH cDNA probe The filters were analyzed by phosphorimaging and hybridization signals were quantified to determine the relative amounts of mRNA (Molecular Dynamics, CA, USA) The mRNA levels ... autoradiograms are shown in the left panels while a graphical representation of the hybridization signals as quantified by phosphorimager scanning is shown in the right panel To compare mRNA expression from ... Alberts, A.S & Sahai, E (1998) Regulation of SRF activity by Rho family GTPases Cold Spring Harb Symp Quant Biol 63, 643–651 Mack, C.P., Somlyo, A.V., Hautmann, M., Somlyo, A.P & Owens, G.K (2001) Smooth...

14
415
0

the accidental death of an anarchist

Ngày tải lên : 21/03/2014, 22:52

3
402
0

Báo cáo khoa học: Identiﬁcation of domains involved in the allosteric regulation of cytosolic Arabidopsis thaliana NADP-malic enzymes ppt

Ngày tải lên : 23/03/2014, 04:20

13
360
0

Báo cáo khoa học: The role of residues R97 and Y331 in modulating the pH optimum of an insect b-glycosidase of family 1 pdf

Ngày tải lên : 23/03/2014, 15:21

... on the pKa values of the b-glycosidase catalytic glutamates still remains to be determined and quantied To ll these gaps, residues Y331, R97 and E187 of Sfbgly50 were replaced through site-directed...

10
522
0

hack the stack - using snort & ethereal to master the 8 layers of an insecure network

Ngày tải lên : 25/03/2014, 11:19

... Valuation 390 Threat Assessment 392 Impact Definition and Quantification 394 Control Design and Evaluation 395 Residual Risk Management...

468
729
0

Báo cáo khoa học: The subcellular localization of vaccinia-related kinase-2 (VRK2) isoforms determines their different effect on p53 stability in tumour cell lines pdf

Ngày tải lên : 30/03/2014, 11:20

... those who will respond to imatinib treatment from those who will not [21] In B cells, analysis by quantitative MS indicates that it is downregulated when Myc expression is induced [22] VRK1 is also ... and puriﬁed isoforms (Fig 3B) The relative concentration of mRNA was also determined by real-time quantitative PCR in the H1299 and FEBS Journal 273 (2006) 2487–2504 ª 2006 The Authors Journal compilation ... VRK2 isoforms were detected in the western blot with a rabbit VRK2-speciﬁc polyclonal antibody (C) Quantitative detection by real time RT-PCR of total VRK2 (isoform A plus B) messages (solid lines)...

18
333
0

numerical analysis of the spectral response of an nsom measurement

Ngày tải lên : 06/05/2014, 08:58

... magnetic ﬁelds are, uE = εE /2 and uH = μH /2, respectively [9] In a propagating wave, these two quantities are equal; however, this is not necessarily true in an evanescent ﬁeld [9] From our near...

9
258
0