Báo cáo khoa học: Lpx1p is a peroxisomal lipase required for normal peroxisome morphology potx

... the peroxisomal aspartate aminotransfer- ase Aat2p in HPLC fraction 7 at a molecular mass of approximately 45 kDa (Fig. 1A) [15]. The predicted molecular mass of Lpx1p is 44 kDa. It carries a peroxisomal ... preparations in triplicate. Candida rugosa lipase (CRL) was used as a positive control for lipase measurement. (Pancreas) lipase activity assays used DGR in a coup...

Ngày tải lên: 07/03/2014, 05:20

... 25 2.2 2 1.8 1.4 1.2 1.6 1 0.8 0.6 0.4 0.2 0 A 600 A 600 A 600 Time (h) Fig. 5. His41 is essential for Paracoc- cus pantotrophus NirF, but Asp129 is dis- pensable. Growth plots and time courses of nitrite appearance and disappearance for P. ... 111–127. 13 Kawasaki S, Arai H, Kodama T & Igarashi Y (1997) Gene cluster for dissimilatory nitrite reductase (nir) from Pseudomon...

Ngày tải lên: 15/02/2014, 01:20

... sulfoxide were added to each well and gently shaken for 10 min. The absorbance was determined at 492 nm with plate reader (Sunrise, Tecan, Gr ¨ odig, Austria). Data analysis Data were analyzed by ... reaction volume with rTaq DNA polymerase or LA Taq TM DNA polymerase with GC buffer (TaKaRa). Human NANOGP8 mRNA was amplified by RT-PCR using total RNAs extracted from urinary bladder cancer ti...

Ngày tải lên: 07/03/2014, 12:20

... http://mips.gsf.de/proj/ yeast/info/tools/hegemann/gfp.html) using the primers SUT-GFPfwd 5¢-GACTGTCGATGATTATGGTTGCC CGCTGGCTTCCAAACCCTTATCGATACCGTCGA CCC-3¢ and SUT-GFPrev 5¢-AACAATTTCACACACA GGAAACAGCTATGACCATGATTACGCTATAGG GCGAATTGGGTA-3¢, ... disRAS1fwd 5¢-TTCACGATTGAACAGGTAAACAAAATTTTCC CTTTTTAGAACGACATGCAGCTGAAGCTTCGTA CGC-3¢ and disRAS1rev CAAAACCATGTCATAT CAAGAGAGCAGGATCATTTTCAACAAATT...

Ngày tải lên: 07/03/2014, 15:20

... probe and a sense cDNA probe (complementary to the antisense) for mouse Slc1 2a2 mRNA were designed as follows; Slc1 2a2 antisense, 5¢-ATCTTCACAAGAAAAAT CACCTGGTACCAAGGATGT; Slc1 2a2 sense, 5¢-ACAT CCTTGGTACCAGGTGATTTTTCTTGTGAAGAT. All ... genes and connective tissue pattern- ing. Development 121, 693–705. 16 Ozaki H, Watanabe Y, Takahashi K, Kitamura K, Tanaka A, Urase K, Momoi T, Sudo...

Ngày tải lên: 07/03/2014, 17:20

... used for generating the five mutants were: C126S, 5¢-TAATTTAAAAGCCGTTGTATCCTTTGGT GAATCTT-3¢; C12 6A, 5¢-TAATTTAAAAGCCGTTGT AGCTTTTGGTGAATCTT-3¢; C126V, 5¢-TAATTTAAAA GCCGTTGTAGTTTTTGGTGAATCTT-3¢; ... site-specific mutagenesis – distal effects on dimer stability Moumita Samanta 1 , Mousumi Banerjee 1 , Mathur R. N. Murthy 1 , Hemalatha Balaram 2 and Padmanabhan Balaram 1 1 Molecular Biophysic...

Ngày tải lên: 14/03/2014, 23:20

... transvacuolar strands and maintain overall cellular architecture. As mentioned above, CRP1 may participate in the formation and ⁄ or maintenance of long actin cables [12]. Consistent with this ... proteins and interacts with skeletal a- actin in the cytoplasm. However, little is known about the mechanism whereby hhLIM interacts with skeletal a- actin and regulates the organization and rea...

Ngày tải lên: 16/03/2014, 06:20

... amylin to evaluate its effect on amylin aggregation. Samples were incubated at 37 °C for 72 h with shaking, and were taken for ThT assays, light scat- tering assays and HPLC analysis at selected ... aggregation may begin to appear. This promotional effect will then lead to enhanced amyloid formation and make amyloid degradation more difficult. We showed that this promotional effect was sig...

Ngày tải lên: 23/03/2014, 04:21

... Seligman PA & Allen RH (1978) Characterization of the receptor for transcobalamin II isolated from human placenta. J Biol Chem 253, 1766–1772. 22 Quadros EV, Nakayama Y & Sequeira JM (2005) ... Aarhus, Denmark 5 Department of Clinical Biochemistry, AS Aarhus University Hospital, Denmark Cobalamin (Cbl, vitamin B 12 ) is a cofactor for two crucial enzymes in mammals [1]. Theref...

Ngày tải lên: 19/02/2014, 05:20

... TCATCTTTTAAACTTTGGGCGAAGGCGTTT 23 TTTTCTCGAGAAAGATGCCGATTTGGGCGC 24 GGGGCTCGAGGTTTTATATTTGTTGTAAAA 25 ATATTATATATATATATAGGGTCGTATATA 26 AAATTATAGAAAGCAGTAGA TAAAACAATG 27 CTTCGAAGAATATACTAAAAAATGAGCAGG CAAGATAAACGAAGGCAAAGTTCAATTCA TCATTTTTTTTTTATTCTTTT 28 ... GCCCGGATCCTGATAGTAATAGAATCCAAA 4 CCCCGAATTCAAATTATAGAAAGCAGTAGA 5 AAGGCTCGAGAGATCTGTTTAGCTTGCCTC 6 AAAAGTCGACGAGCTCGTTTTCGACACTGG 7 TT...

Ngày tải lên: 16/03/2014, 01:20