use of e coli for the production of a single protein

Báo cáo khoa học: Dynamin-like protein-dependent formation of Woronin bodies in Saccharomyces cerevisiae upon heterologous expression of a single protein pdf

Ngày tải lên : 23/03/2014, 07:20

... matrix proteins For example, evidence for the appearance of some GFP-SKL or Cta1p was gathered in several of our localization experiments The density of these structures could thereby be lowered without ... vesicles attached Based on the gathered data, these vesicles were likely to represent peroxisomes To support this hypothesis, immunoﬂuorescence studies were performed Extended single peroxisomes were ... different orientation with respect to the plane of the section The short dimension ﬁts with the measurement of the en face view of the hexagonal structures In some rare cases, peroxisomes with areas...

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The Use of Plant Cell Biotechnology for the Production of Phytochemicals

Ngày tải lên : 25/10/2013, 05:20

... sufﬁcient visualization of the entire metabolome Multiple technologies are therefore needed to measure the entire metabolome of a given plant sample Most metabolomic approaches seek to proﬁle metabolites ... that are based on the conversion of an endogenous metabolite There are several examples that have been reported for well-characterized rate-limiting enzymes of plants and their controversial ... >50,000-fold range, and the isolation of mechanistically informative prephenate dehydratase variants from large protein libraries illustrate the potential of the engineered selection strain for characterizing...

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Tài liệu GUIDELINES FOR THE PRODUCTION, PROCESSING, LABELLING AND MARKETING OF ORGANICALLY PRODUCED FOODS docx

Ngày tải lên : 14/02/2014, 03:20

... being of the livestock having regard for the species, the breed and the age of the livestock; – take into account the behavioural needs of the livestock with respect to the size of the group and ... satisfying the requirement outlined in paragraph 13 above are not available, as a result of, for example, unforeseen severe natural or manmade events or extreme 13 GL 32-1999 climatic weather ... produce the food, in the case of processing aids in the absence of other available technology that satisfies these Guidelines; – these substances are found in nature and may have undergone mechanical/physical...

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Tài liệu GUIDELINES FOR THE PRODUCTION, PROCESSING, LABELLING AND MARKETING OF ORGANICALLY PRODUCED FOODS pdf

Ngày tải lên : 18/02/2014, 07:20

... being of the livestock having regard for the species, the breed and the age of the livestock; – take into account the behavioural needs of the livestock with respect to the size of the group and ... satisfying the requirement outlined in paragraph 13 above are not available, as a result of, for example, unforeseen severe natural or manmade events or extreme 13 GL 32-1999 climatic weather ... produce the food, in the case of processing aids in the absence of other available technology that satisfies these Guidelines; – these substances are found in nature and may have undergone mechanical/physical...

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Tài liệu Novel Design of an Integrated Pulp Mill Biorefinery for the Production of Biofuels for Transportation pot

Ngày tải lên : 26/02/2014, 14:20

... has a large heat capacity The bubbles agitate the oil solvent, and because the oil solvent has a high heat transfer rate, temperatures inside the reactor are maintained uniform, making it easier ... consumption has outpaced newfound reserves Therefore, great efforts in research and development have been made into new vehicle technology and new fuels A means of reducing or eliminating the dependency ... the catalyst is suspended in a reaction medium Because the heat capacity and thermal conductivity of the reaction medium are both large, the reaction heat is absorbed by the reaction medium and...

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The bioliq® bioslurry gasification process for the production of biosynfuels, organic chemicals, and energy pdf

Ngày tải lên : 14/03/2014, 19:20

... melting point at > 1,000°C and generate a practically tarfree, low-methane raw syngas Because of the higher temperatures in an EF gasifier, a cleaner syngas is obtained at the expense of more ... gives a brief overview The main difference between these process variants are the biomass pretreatment steps Pretreatment for PEF gasifiers requires more technical effort than that for fixed ... like formaldehyde, acetaldehyde, acetone, methanol, glyoxal, methyl, ethyl esters of formic and acetic acids, etc can escape with the gases, but have not been analyzed in detail so far They are...

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Báo cáo khoa học: "AN EXPERT SYSTEM FOR THE PRODUCTION OF PHONEME STRINGS FROM UNMARKED ENGLISH TEXT USING MACHINE-INDUCED RULES" pdf

Ngày tải lên : 24/03/2014, 05:21

... is evaluated and the proper branch is then selected on the basis of values of the word features The process is repeated until a leaf node of the tree is reached The leaf node contains the value ... The training mode operates in the same manner as the execution mode with the exception that, whenever either rule is applied (see steps and above), the user is prompted for a judgement on the accuracy ... any other example whose feature values are known or extractable I0 The training set now consists of all of the entries from the SCL and the SEL, as well as all of the features for each entry At...

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Cocoa Processing Methods for the Production of High Quality Cocoa in Vietnam ppt

Ngày tải lên : 21/06/2014, 06:20

... leaves and jute bags are recommended Farmers should obtain a thermometer to measure the temperature on each day of the fermentation The best results are obtained in fermentations where the maximum ... the count of defective beans in the ‘cut test’ The cut test reveals the presence of certain defects which may cause off-flavours and indicates the degree of fermentation of the beans which has ... temperature reached is between 45oC to 50oC As a general rule, the closer to 50oC, that fermentations reach, the better the quality of the dried cocoa is Typical temperatures for the different...

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Báo cáo khoa học: " Effective methods for the production of immunoglobulin Y using immunogens of Bordetella bronchiseptica , Pasteurella multocida and Actinobacillus pleuropneumoniae" docx

Ngày tải lên : 07/08/2014, 15:20

... of experimental vaccines in hens Experimental vaccines were made using antigens prepared as described above Vaccine formulations are shown in Table Twenty-week-old white laying hens purchased ... important infectious pathogens in the porcine respiratory tract Although several attempts have been made to control these agents in swine industry worldwide, the diseases caused by these bacteria ... by Western blot and challenge test in mice, respectively However, some problems were experienced, such as depression, weakness, a reduction in the laying rate, and increased mortality, especially...

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Báo cáo khoa học: " Efficacy of VP2 protein expressed in E. coli for protection against highly virulent infectious bursal disease virus" pot

Ngày tải lên : 07/08/2014, 18:21

... ro/dna egahrromeh ,amede lasrub ereves ot etaredom yb deziretcarahc noitcefni VDBIvh suoivbo dah snekcihc daed eht fo lla taht demrifnoc sisylana metrom-tsoP snoitanimaxe lacigolohtapotsih dna metrom-tsoP ... eniccaV seniccav namuh dna yraniretev rof dengised stnavujdA V ennaG ,L siupuD ,J reirutuocuA iloc aihcirehcsE secnerefeR nietorp 2PV tsniaga seidobitna eht gnidivorp rof ynamreG ,slaminA fo sesaesiD ... noitamrofnoc negitna eht taht stseggus yduts tnecer A yhporta lasrub tsniaga noitcetorp ecudni ot laitnesse si ,suriv evil ni tneserp era taht sepotipe lanoitamrofnoc etairporppa eht tsniaga ,sretit...

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Báo cáo y học: "Use of a multi-virus array for the study of human viral and retroviral pathogens: gene expression studies and ChIP-chip analysis" pot

Ngày tải lên : 13/08/2014, 13:20

... We treated the Cy3 and adjusted Cy5 intensities as technical replicates and calculated the mean of these values The ratio of this mean on the average of the intensity across the array set was ... intensities was calculated for both the experimental and control and the ratio of these was used as a scaling factor to adjust the values of all fluorescence measures for the viral genes represented ... regulated by Rev and therefore these transcript levels accumulate in the absence of Rev protein and are down regulated in the presence of Rev [38] When cells were treated with CYC202, we observed...

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standard specification for producing a skid-resistant surface on concrete by the use of a multi-component epoxy s

Ngày tải lên : 24/10/2014, 22:12

... coating rate of application specified in Article 2.3.4.1 If either of these is changed the aggregate application rate may have to be adjusted and a sample should be prepared to evaluate the resulting ... the proposed aggregate and coating at their proposed rate of application prior to publication of a specification will enable the designer to anticipate the appearance and texture of the surface ... Standard Test Method for Resistance to Degradation of Small-Size Coarse Aggregate by Abrasion and Impact in the Los Angeles Machine Standard Method for Sieve Analysis of Fine and Coarse Aggregates...

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Investment project analysis of e customs for the authorized economic operator

Ngày tải lên : 24/11/2014, 01:07

... hardware replacement, there are items need to be maintained annually, that called operating cost (example: expense for maintenance, power consumption, office rental); these expenses should be considered ... to get the result green lane, green lane, red Three lanes: green lane, yellow lane, lane, but yellow lane, red lane red lane normally is green lane Archiving Freight supplier has to keep in All ... scale of the freight forwarder, and scale of some other foreign freight forwarders in market; table 3-14 also reflects the requirement of man power from relevant department during the implementation...

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Advantages and challenges of the spray drying technology for the production of pure drug particles and drug loaded polymeric carriers

Ngày tải lên : 02/09/2015, 13:31

... limitation of the device is that the PT vibrating mesh causes mechanical shear of atomization and shear-sensitive substances RI can be altered [65] Heng et al reviewed on the advances of Nano Spray ... point For example, the use of MA dichloromethane (40C) or acetone (56C) avoids problems of sticking and agglomeration The reason is that they evaporate at a temperature that is lower than the D melting ... increases the MA collection extent Furthermore, these MPs are more physically stable than the NPs because the smaller size of the NPs could lead to strong inter-particle interactions and D subsequent...

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Microencapsulation of clostridium acetobutylicum cells and utilisation of samanea saman leaves for the production of biobutanol

Ngày tải lên : 08/09/2015, 18:41

... have been associated due to the large size of the beads For instance, low cell viability has been reported at the centre of the larger beads after a relatively short operation time due to depletion ... is also affected by the presence of cations (Bajaj et al., 2007) There are two main types of gellan gum, the acetylated and the deacetylated gellan gum Acetylated gellan gum is obtained by partial ... year The removal of this waste leaf litter is either done manually or mechanically The collected leaves are then either used as manure, animal feed or destroyed in incinerators by the local authorities...

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Báo cáo khoa học: Differential tissue-speciﬁc distribution of transcripts for the duplicated fatty acid-binding protein 10 (fabp10) genes in embryos, larvae and adult zebraﬁsh (Danio rerio) docx

Ngày tải lên : 16/03/2014, 00:20

... fabp1 0a cDNA sequence [22] as a query, a gene sequence (ENSDARG00000069449) and a transcript (ENSDART00000101095) were retrieved from a blastn search of the zebraﬁsh genome sequence database at the ... transcripts determined in each tissue sample, and represented as the relative abundance of mRNA for the fabp10b gene in each tissue Acknowledgements The authors wish to thank Dr Marc Ekker for the provision ... gene, is a duplicated copy of the previously described zebraﬁsh fabp10 (hereafter referred to as fabp1 0a) gene These duplicated copies of the fabp10 gene most probably arose as a result of a...

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The German Implementing Act for the AIFM Directive: A Critical Survey of the Draft Bill ppt

Ngày tải lên : 23/03/2014, 06:20

... European level On the contrary, there are already further drafts of directives and regulations in the area of investment and capital market law at the European level Some specific examples would ... restriction of external financing to 30% of the fund capital Grandfather Clause for Existing Open-ended Real Estate Funds For real estate investment funds established before the time of the cabinet decision ... which — separated from the investment capital — encompass the capital required for the management of operations The partnership agreement of an open-ended InvestmentKG may permit the creation of sub-funds...

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Báo cáo khoa học: A strategy for the generation of speciﬁc human antibodies by directed evolution and phage display An example of a single-chain antibody fragment that neutralizes a major component of scorpion venom docx

Ngày tải lên : 23/03/2014, 13:20

... cells The clones selected with this procedure were evaluated by ELISA as soluble proteins Expression of single- chain antibodies The scFv inserts from the selected clones, were ligated into the ... displayed on ﬁlamentous phages The scFv 6009F could be used as a potential component of a recombinant antiserum against Centruroides stings These results open new avenues for the generation of recombinant ... fraction was used The BIACORE analysis (Fig 3; Table 2) showed a KD value of 410 pm, the best afﬁnity value for the evolved variants Table Neutralization assays Results of mice groups challenged...

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Báo cáo khoa học: " Development and Use of a Gold-Standard DataSet for Subjectivity Classifications" pdf

Ngày tải lên : 23/03/2014, 19:20

... conditionally independent of one another, given the value of the latent variable The latent variable represents the true state of the object, and is the source of the correlations among the observed ... observed variables As applied here, the observed variables are the classifications assigned by the judges Let B, D, J, and M be these variables, and let L be the latent variable Then, the latent class ... Kappa values, and raises the average agreement among the judges to a Kappa value of over 0.87 for the sentences that can be tagged with certainty Using only simple features, the classifier achieves...

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báo cáo hóa học: " A pilot study evaluating use of a computer-assisted neurorehabilitation platform for upper-extremity stroke assessment" pptx

Ngày tải lên : 19/06/2014, 08:20

... Success Percentage The percentage of the targets that have been successfully reached by the subject Overall Reaching Capability Error_Mean The mean of the displacement outside of the holding area ... Reaction Quickness Movement Time The time window from the end of the reaction time to the time after which the user has stably reached the target Movement Quickness Deviation The mean of the ... UniTherapy applied none or varying levels of force-feedback to physical therapeutic interfaces, depending on the settings and the task; these were derived from a series of force effects such as...

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