structural basis for the stratum corneum barrier

Báo cáo khoa học: Structural basis for the erythro-stereospeciﬁcity of the L-arginine oxygenase VioC in viomycin biosynthesis docx

Ngày tải lên : 16/03/2014, 02:20

... molecules of l-serine (Fig 1A) Another striking feature of these NRPSs is related to the C-terminus of the synthetase VioG Although there is no need for a further condensation reaction, this NRPS ... site formation after substrate binding Interestingly, the disorder of the lid region appears to be increased in the product rather than in the substrate complexes In the VioC•hArg complex, the ... substrate-binding mode results from conformational control of the enzyme on the side chain rotamer of the bound substrate For example, in AsnO, a trans conformer is selected for the v1 torsion angle of bound...

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Báo cáo Y học: Structural basis for the inhibitory efﬁcacy of efavirenz (DMP-266), MSC194 and PNU142721 towards the HIV-1 RT K103N mutant doc

Ngày tải lên : 18/03/2014, 01:20

... by the K103N mutation The electron density for Cc of the K103N mutant structures is well deﬁned but the quality of the map does not allow assessment of the orientation of the amide plane There ... contact is the hydrogen bond formed between the NNRTIs and the backbone carbonyl of K101 Omit electron density maps of the RT/NNRTI complexes clearly show the orientation and conformation of the inhibitors ... (Table 3) The effects of the K103N mutation on the hydrophobic interactions reveal individual differences among the three compounds Though the electron density for the amide part of the residue...

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Báo cáo khoa học: Structural basis for the recognition of complex-type biantennary oligosaccharides by Pterocarpus angolensis lectin ppt

Ngày tải lên : 23/03/2014, 11:20

... complexes [34] Therefore, we conclude that the reason for the difference in energetics of the )1 subsite between PAL and A must be due to details in the interaction between the protein and the carbohydrate ... subsites will occur Thermodynamics of carbohydrate binding to PAL To complement our structural studies, the thermodynamic parameters for the binding to PAL for mannose, GlcNAcb(1–2)Man, the pentasaccharide ... binding in the global minimum conformation Neither in the GlcNAcb(1–2)Man complex, nor in the complexes with M592 or GlcNAcb(1–2) Mana(1–3)ManaMe is there any evidence for two conformations despite...

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Báo cáo khoa học: Structural basis for the interaction between dynein light chain 1 and the glutamate channel homolog GRINL1A docx

Ngày tải lên : 29/03/2014, 09:20

... Figure 4A shows the 20 lowest-energy conformers from the selected cluster modelled using the Haddock docking calculations The rmsd values for the 20 lowest-energy conformers of the best cluster ... 0.71 ± 0.11 A for the backbone and 1.11 ± 0.09 A for the heavy atoms The mean total energy of the ensemble is )8146 kcalÆmol)1, being )314 kcalÆmol)1 the intermolecular contribution The corresponding ... used for the peptide (C) Lowest-energy conformer of the family, using ribbon and stick representations for the protein and the peptide, respectively Selected side chains with important roles in the...

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Báo cáo khoa học: Structural basis for the changed substrate speciﬁcity of Drosophila melanogaster deoxyribonucleoside kinase mutant N64D docx

Ngày tải lên : 30/03/2014, 20:20

... bound to the 3¢-OH group of the substrate while there is no density for that interaction in the mutant structure (Fig 2B) Structural basis for altered properties of the N64D mutant The LID region ... is therefore a particularly attractive candidate for the medical gene therapy applications mentioned above, as well as for industrial synthesis of d(d)NTPs and their analogs [6,15] To further ... with the substrate dT, in red, and with AZT modeled in the substrate binding site, in blue The interactions with the substrate are the same in the wildtype and the N64D mutant except for the lack...

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Báo cáo khoa học: A structural basis for the pH-dependence of coﬁlin F-actin interactions potx

Ngày tải lên : 31/03/2014, 09:20

... by others [31], and the thrusting forward of subdomain with respect to the rest of the monomer In this report we study the pH-dependence of the actin– cofilin interface and provide evidence for ... decrease in the cofilin binding in the presence of actin suggesting that the actin–cofilin complex impedes the interaction of cofilin with the actin peptide The complex formation between the C-terminal ... environment with the antigenic epitope located within cofilin site in actin sequence Evidence for a change in the conformation of actin in the 75–103-actin region DISCUSSION The significance of the modifications...

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Structural basis for the inhibition mechanism of HUman CSE and a study on c CBL complexes

Ngày tải lên : 11/09/2015, 10:17

... absorption 46 for the PLP-lysine internal aldimine bond However the bound PLP of the GST-tagged purified hCSE was most likely lost during crystallization For the apo-hCSE, rod-shaped crystals with the ... were present in the asymmetric unit Data in the interval 19.9-2.0 Å resolution was used and at the end of the refinement the R-value was 0.158 (Rfree= 0.204) for all reflections For both hCSE-PLP ... was then plotted against the cysteine substrate concentration, [S] and fitted against the Hill equation, 50 The Hill coefficient, h was determined from the gradient of the logarithmic plot of the...

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Báo cáo khoa học: The structural basis for catalytic function of GMD and RMD, two closely related enzymes from the GDP-D-rhamnose biosynthesis pathway pdf

Ngày tải lên : 16/03/2014, 01:20

... requires the formation of an initial enzyme–NAD(P)H complex, whereupon the cofactor is oxidized to NAD(P)+ during the reaction Therefore, the reduced cofactor must be replaced from the solution before ... reaction was performed for h, the enzyme was removed by filtration, and then enzyme and cofactor for the second reaction were added To determine the optimal pH for enzyme-catalyzed reactions, the extent ... nearly identical to those reported for GDP-d-Rha [5] On the basis of these NMR findings, compound D was concluded to be GDP-a-d-Rha These results for His6-RMDPa therefore confirm that this enzyme is...

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Tài liệu CATIA V5 Structural Analysis for the Designer P2 doc

Ngày tải lên : 25/01/2014, 15:20

... Step Visualize the analysis results Visualize the finite element mesh in the deformed state of the system as a result of loading Steps: Select the Deformation Image Icon 2 Note the Deformed Mesh ... Apply a distributed force Steps: Select the Force icon Select the outside foot grip pads Enter -100 lbs in the Z-direction, select OK Note the Distributed Force object added to the features tree ... Steps: Select the Stress Von Mises icon Note the Image Deactivated symbol for the Deformed Mesh image CAT509, Workshop 3, March 2002 WS3-16 Step Visualize the analysis results Visualize the displacement...

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Tài liệu CATIA V5 Structural Analysis for the Designer P1 pptx

Ngày tải lên : 25/01/2014, 15:20

... concern is the Foot Peg for the passenger on the ATV The Foot Peg needs to be small due to limited space on the ATV yet able to handle the force of the passenger during the ride Analyze the Foot ... document that will contain the information for our static analysis of the Foot Peg Steps: Select the GSA workbench from the Start menu Highlight the Static Analysis case Click OK The new CATAnalysis ... Apply a force load of 550lbf to the Foot Peg top face in a direction normal to the face pushing downward Steps: Select the force icon from the GSA workbench Drag and drop the compass on to the top...

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Tài liệu Báo cáo khoa học: Structural basis for cyclodextrin recognition by Thermoactinomyces vulgaris cyclo⁄maltodextrin-binding protein ppt

Ngày tải lên : 19/02/2014, 00:20

... all of the conformations are the closed form, were compared (Fig 7A–C) The cleft of the sugar-binding site of TvuCMBP is the widest among the three sugar-binding proteins (Fig 7A) Although the side-chain ... the specificity of the bacterial sugar-binding proteins should be defined in terms not of the affinities for sugars but of whether the protein adopts the open form or the closed form TvuCMBP–c-CD ... illustrated shows the high affinities for not only CDs but also higher maltooligosaccharides It is impossible to determine, however, whether TvuCMBP adopts the open form or the closed form with the sugars...

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Đề tài " A five element basis for the uncountable linear orders " doc

Ngày tải lên : 06/03/2014, 08:21

... element basis In fact such a basis follows from the Proper Forcing Axiom, a strong form of the Baire Category Theorem The elements ∗ are X, ω1 , ω1 , C, C ∗ where X is any suborder of the reals ... cardinality ℵ1 , then X serves as a single-element basis for the class of uncountable separable linear orders PFA is a strengthening of the Baire Category Theorem and is independent of the usual axioms ... versed in set theory and the major developments in the ﬁeld in the 70s and 80s The reader is assumed to have proﬁciency in the areas of Aronszajn tree combinatorics, forcing axioms, the combinatorics...

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Báo cáo khoa học: NMR and molecular dynamics studies of an autoimmune myelin basic protein peptide and its antagonist Structural implications for the MHC II (I-Au)–peptide complex from docking calculations ppt

Ngày tải lên : 07/03/2014, 16:20

... among the peptide antigens are indicated in red) The alignment of MBP(74–85) was performed based on the highest aligned score with the three antigens, on the basis of the amino-acid preference for ... sclerosis For a better understanding of the molecular basis of the MBP(74–85) antigen– MHC II recognition, a model for the 3D structure of the MBP(74–85) antigen–MHC II complex is required The only ... of the peptide The side chain of Arg78 is locked into a conformation resembling its conformation in aqueous solution, i.e perpendicular to the plane deﬁned by the backbone of the peptide The...

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Báo cáo khoa học: Structural basis for recognition of Co2+ by RNA aptamers pot

Ngày tải lên : 16/03/2014, 06:20

... oligomers hybridize to the 5¢-end of the loops and propagate to nucleotides involved in the formation of the helix The strong preference for the 5¢-side of the loops in comparison with the 3¢-side may ... are labeled on the right Sites of interference are denoted with arrows moieties in the formation of the Co2+ binding site architecture in the aptamers Therefore, replacement of the nonbridging ... and A41 conﬁrm that the N7 atom of these adenine bases is involved in the formation of nonstandard H-bonding Additionally, in the proposed loop E-like motif structure, the aforementioned N7 position...

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Báo cáo khoa học: Structural basis for substrate recognition by Erwinia chrysanthemi GH30 glucuronoxylanase potx

Ngày tải lên : 22/03/2014, 16:20

... takes place at the second glycosidic linkage from the MeGlcA side group towards the reducing end of the xylan chain The elucidation of the three-dimensional structure of the E chrysanthemi GH30 enzyme ... emerged concerning the basis for the recognition of the MeGlcA and GlcA residues by the enzyme We have postulated an ionic interaction between the uronic acid carboxylate and the positively charged ... however, the best diffraction data were recorded with the crystal of the complex obtained by cocrystallization (Fig S3C) These data are reported here The crystals of the complex belonged to the P3221...

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Báo cáo khóa học: Acyl-CoA dehydrogenases and acyl-CoA oxidases Structural basis for mechanistic similarities and differences pot

Ngày tải lên : 23/03/2014, 12:20

... to the active-site and the dimeric structure rather than a tetrameric form and (b) the si-face of the ﬂavin ring in ACO being further away from the surface of the molecule than in MCAD due to the ... required for the decarboxylation reaction Thus, Arg94 appears to be involved in the binding of the substrate and in the alignment of the glutaryl-CoA substrate for optimum orientation for the dehydrogenation ... addition, the positive charge of Arg94 appears to be involved in the stabilization of the anionic intermediate, crotonyl-CoA anion, during catalysis The exact mechanism and the structural basis for the...

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Báo cáo khoa học: Structural basis for stereo-speciﬁc catalysis in NAD+-dependent (R )-2-hydroxyglutarate dehydrogenase from Acidaminococcus fermentans pptx

Ngày tải lên : 23/03/2014, 13:20

... clariﬁed how the protein backbone controls the substrate stereorecognition for each member of the protein family as their substrates range from the small formate (nonchiral) up to the more structurally ... at the surface of the protein were modelled according to the more stable conformer The electron density for the cispeptide bond between residues Tyr289 and Pro290 is clearly deﬁned Statistics for ... red for arginine, blue for glutamate and aspartate, green for histidine, cyan for polar, orange for aromatic and aliphatic side chains, brown for Thr77–Gly79 and dark red for Gly153-Asp176 The...

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Báo cáo khoa học: "A Logical Basis for the D Combinator and Normal Form in CCG" pptx

Ngày tải lên : 31/03/2014, 00:20

... two theorems about the set of semantically equivalent parses that a CCG parser will generate for a given string (see (Eisner, 1996) for proofs and discussion of the theorems): (31) Theorem : For ... Wallace) ate)? Finally, the unary combinator basis for CCG provides an interesting additional speciﬁcation for generating CCG rules Like the CTL basis, the unary combinator basis can produce a much ... de los Anillos cl.1p made.3s read the Lord of the Rings “He made us read The Lord of the Rings.” The aspect of the construction that is relevant here is that the causative verb hacer appears to...

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Báo cáo khoa học: Structural requirements for the apical sorting of human multidrug resistance protein 2 (ABCC2) potx

Ngày tải lên : 31/03/2014, 09:20

... (bold), at the same time removing the stop codon and the BsrGI site at the 3¢ end of the GFP coding sequence PCRampliﬁed GFP was cloned into the pDisplay vector (Invitrogen) using the BglII and the ... in the mammalian expression vector pcDNA3.1(+) (Invitrogen) After translation, GFP was attached to the N-terminus of the proteins, so that the GFP moiety was in the lumen of the ER or on the ... the MRP1 cDNA, inserted into the vector pcDNA3.1(+), as template and the T7 vector primer as forward primer The reverse primer ochimrp1.rev was used to generate the XbaI restriction site in the...

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Báo cáo khoa học: Structural basis for poor uracil excision from hairpin DNA An NMR study pptx

Ngày tải lên : 31/03/2014, 09:20

... of the four, is located in an environment wherein both the backbone and the base conformation mimic the B-form of DNA Thus the structural features of the U2-hairpin provided an explanation for ... addition, the protrusion of the U towards the minor groove side of the hairpin stem may also lead to steric hindrance in the approach of UDG to DNA On the other hand, U15 in the U4-hairpin, the best ... straightforward for both of the hairpins The degeneracy between these H6 protons could be resolved by the observation of intra-nucleotide and sequential nOes Ó FEBS 2002 Structural basis for poor...

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