... shift andthe hyperfine field However, due to the poor statistics, the spectra were fitted only with a distribution of hyperfine fields The fine structureofthe fitted spectra is probably due to ... are stronger than those in the ‘pure’ a-RFe and a-RCo alloys This was thought to be the reason for the closing ofthe cone type distribution of (Tb, Dy) moments, then the enhancement ofthe R ... between the incident γ -ray direction (being the direction normal to the film) and that ofthe hyperfine field Bhf (or the Fe magnetic moment direction) is estimated from the line-intensity ratios...
... oxidation The fraction ofthe magnetostrictive alloy ͑18% ofthe total spectrum area͒ is small As already mentioned in the beginning of this subsection, this reflects that the thickness ofthe oxidation ... magnetization of M without any contribution to magnetostriction In the second step, the spins rotate in the direction ofthe applied magnetic field, leading to the change of both magnetization and magnetostriction ... to the strong local anisotropy ofthe R atoms and their random distribution of easy axes present in such sperimagnetic systems, the coercivity is strongly affected by internal stress, microstructure,...
... catalytic activity If the substrate recognition site andthe catalytic site in the same polypeptide were responsible for catalyzing the lipid substrate, which is the expectation ofthe head-to-head ... domain of one subunit andthe substrate recognition site in the C-terminal domain ofthe other face each other, thereby bringing together the substrate-binding and catalytic functions in proximity ... configuration after cotransfection of inactive LPL mutants and recovery of catalytic activity (A) Lipase activity is not detected in COS7 cells transfected with only the S13 2T or the WWW mutant If the...
... andT are the gas constant andthe absolute temperature, respectively In order to estimate the conformational stability (DGH2 O ) U of frutalin, it was assumed that the linear dependence ofthe ... Although the transition curve of frutalin without this sugar ð2Þ The observed maximum fluorescence emission ofthe protein at any concentration ofthe denaturant is given by the sum ofthe contributions ... fluorescence intensity observed for Mfrutalin contrasted with that of Ufrutalin, possibly due to the fact that Trp is buried in the particular folding ofthe Mfrutalin structure, or is inserted into a...
... 5¢-ATTTCTGTATTCAAGCTGGAGGCAGAGCCGTG AT-3¢, antisense 5¢-CTGCCTCCAGCTTGAATACAG AAATG-3¢; N424D, sense 5¢-AGATTTGGGGATAC TTCATCTAGCTCAATTT-3¢, antisense 5¢-AGATGAAGT ATCCCCAAATCTATGTAACG-3¢; N424H, sense 5¢-AGATTTGGGCATACTTCATCTAGCTCA-3¢, ... decarboxylation activity and characterization ofthe mutants ofthe putative catalytic triad strongly support the hypothesis that the membrane-bound FAE1 KCS shares the same basic mechanism with the ... significantly reduce the decarboxylation activity ofthe chalcone synthase, thus indicating that acylation ofthe active-site cysteine is not essential for decarboxylation of malonyl-CoA substrate Therefore,...
... duration of therapy, information about patients studied and their diagnoses, study design, the number of patients with efficacy and/ or safety outcomes, and discontinuations, especially because of ... [18] transplants This provides additional reassurance of safety and tolerability of MMF, often at high doses (like the g daily in the heart transplant trial) and over a treatment duration of up to ... events by using as the denominator the total number of patients in studies mentioning the event For randomised trials in which MMF was compared with another treatment, relative benefit and relative...
... study, in the development and conduct of analyses, and in the clinical trials and data collection WLM, JJ, and DRN participated in the conception and design ofthe study, in the development and ... absolute reductions in mortality may not overcome the complications of therapy In ADDRESS, as in the PROWESS study, there was a statistically significant interaction between the observed treatment ... mortality rates between treated and placebo patients Results At the time of termination, 2,640 patients had been enrolled in the ADDRESS study at 516 centers in 34 countries Mortality data at...
... both? The goal of asking these questions is not to exclude any sort of physician from the community of intensivists, but rather to work toward identifying the optimal way to train intensivists ... accepted that most ofthe opportunities to improve the performance of complex organizations derive from improving the structures and processes that they consist of Although this vital concept is often ... optimal duration of training? What is the optimum training curriculum? Are outcomes of certain types of patients better under the care of intensivists with a certain base specialty or ICU training...
... Guyatt's Statistic Overall Problem Mean Change Guyatt's Statistic Stigma Mean Change Guyatt's Statistic Treatment Satisfaction Mean Change Guyatt's Statistic COE -t questionnaire scales at adjacent ... for their help in the preparation of this manuscript The validation study design, analysis, interpretation of results, the writing ofthe manuscript represent the joint collaboration of all authors ... study, performed the statistical analysis, and drafted the manuscript SDM assisted in the design ofthe validation study, participated in the design ofthe statistical analysis, and helped to...
... Overall structureThestructureof full-length human PRTFDC1 was ˚ determined at 1.7 A resolution with two subunits in the asymmetric unit Most ofthe polypeptide chains could be traced in theelectron ... substrate and product Phosphoribosyltransferase activity was detected with hypoxanthine and guanine and there was no detectable activity with adenine Subsequently, the kinetic constants were determined ... amount of products was quantified directly, and therefore it is a more accurate and sensitive method compared to the spectrophotometric methods Because the enzymatic activity of PRTFDC1 towards hypoxanthine...
... synthesis of pantothenate from low amounts of pantoate [9] Here, we report the results ofthe structural studiesofthe pantoate-bound form ofthe N-terminal domain of E coli PS (nPS) Thestructure ... which the second molecule of pantoate binds to the pantoyl adenylate-bound form ofthe protein, at a site other than the active site, which then has a regulatory role in the kinetics ofthe reaction ... important to note that, at the level of sensitivity and resolution available for these studies, pantoate was not found to bind to any other site on the protein, leading us to conclude that there...
... experimental thermograms without fitting to any particular model The thermogram yields the overall enthalpy directly as the integral ofthe thermogram: Z 1ị DH ẳ Cp dT The overall entropy may be calculated ... understand such allosteric effects, the energetics ofthe underlying conformational transitions in quadruplex structures must be determined The thermodynamics and kinetics ofthe transition ofthe ... rearrangements to generate the final K+-bound product(s) CD studies showed that addition ofthe porphyrin TmPyP4 promoted conversion ofthe basket to the hybrid form The kinetics ofthe TmPyP4-induced...
... time t, A and k are the amplitude andthe observed rate constant, respectively, and F1 is the fluorescence at infinite time The validity ofthe fitting was evaluated by an inspection of residuals and ... (Poznan, Poland) The DNA sequences are as follows: 5¢-AAAAGTGTGACATGGAATAAATTAGT-3¢ for gal (26 bp), 5¢-ATTAATGTGAGTTAGCTCACTCATTA3¢ for lac (26 bp), and 5¢-AATTAATGTGACATATGTCA CATTAATT-3¢ for ... spectrum at 338 nm These two forms ofthe protein account for 55% and 45% ofthe total fluorescence emission, respectively In contrast to the Trp13 residue, the tryptophan located at position...
... However, the crystal structureofthe Phe35fiTyr mutant shows that the ˚ hydroxyl oxygen atom ofthe side chain of Tyr35 is 3.21 A away from the atom CHB ofthe pyrrole group ofthe heme, making strong ... concentration constant increased sharply after the urea concentration exceeded M These results reflect the tightness ofthe heme attaching to the polypeptide of cyt b5 For the Phe35fiTyr mutant the ... Waals’ contacts with the heme Obviously, the introduction of hydroxyl group in the heme pocket strengthens the interactions between Tyr35 andthe heme with the iron in the oxidative state The increased...
... complementation studies demonstrate that photobacterial sPPases are functionally equivalent to that ofthe host organism, and that the promoters seem to be regulated by the same factors Analysis ofthe ... (Table 1) They display, however, greater heterogeneity in the molecular mass ofthe detected protein band, as is also the case for metal cation dependence, as the sPPase activity of many of these ... Pyrophosphatases from photosynthetic bacteria Results and Discussion Detection of sPPase activity in photosynthetic prokaryotes ) enzymatic features of isolated sPPases Family I is the most widespread and...
... surface ofthe pellets andthe thermalcouple to become liquid-phase catalytic growth center The third step is that the vapor of silicon and silicon monoxide is transported to the catalytic center to ... stability is only relative Amoeba-shaped nanowires are attributed to monocentric nucleation and periodic unstable growth to exhibit a typical periodic instability of diameter The block dots of ... deposit on the surface ofthe trunks to become many new growth centers, namely, polycentre, resulting in the formation of branch-shaped nanowires The branch-shaped nanowires can grow stably, but the...
... 3(a)) and no metal particles are seen at either end ofthe wires The TEM investigations ofthe material further confirm our view that growth of nanowires is essentially non-catalytic in the present ... present study are amorphous across the diameter instead of crystalline Si core andamorphous SiO2 outer shell structure as confirmed by HRTEM image and SAED pattern On the other hand, the formation ... deposited on Ni-coated Si wafers The nanowires have diameter in the range 30–100 nm with center ofthe distribution at $50 nm The diameters remain nearly constant throughout the length ofthe nanowires...
... shows the high-magnification TEM image of a catalyst tip, and Figs 2(c) and (d) are the corresponding SAED and high-resolution TEM image ofthe catalyst, respectively The catalyst is crystallized and ... speculate that the peak shifts for nanowires with different diameter are due to the fabrication temperature, at which these nanowires grow The temperature directly affects the chemical reactions ... diameter of 50 nm is too thick to show any distinct quantum effect on PL Thus the relatively small shift in emission peaks should not be directly related to size reduction There are several nanostructure...
... C-terminus), one at the N- andthe other at the C-terminus; this is sufficient to ensure the cationic nature ofthe peptide at its terminus Thestructureof BM-1 is very compact, like the PG-1 structure, ... differences between the structural families of these peptides applied mainly to the varied structures ofthe N- and C-termini All the features ofthe structures calculated for all three peptides were ... The distribution of hydrophobic and hydrophilic residues at the peptide surface is a structural feature required for the cytolytic activity of PG-1 Structure activity relationship studiesof several...
... formation of a loop that protrudes away from the protein surface into the solvent There is yet another interesting feature ofthe XAIP structure which is related to the conformation of loop b3–a3 This ... unlikely to change to accommodate the substrates This means that the size ofthe carbohydrate-binding channel is not only reduced in width, but is also terminated at the subsite just before the ... respectively XAIP characteristic loop The structural determination of XAIP revealed the presence of a novel loop that protrudes sharply away from the surface ofthe protein The longest helix a3 in the structure...