regulation of glucocorticoid receptor signaling and the diabetogenic effects of glucocorticoid e

Báo cáo khoa học: Subcellular compartmentalization of FADD as a new level of regulation in death receptor signaling pdf

Ngày tải lên : 07/03/2014, 02:20

... receptor engagement is not dependent on caspase activation, and a significant proportion of the surface expression of CD95 observed at 30 and 60 following receptor engagement in the presence of ... for the indicated times, and surface CD95 expression was assessed by FACS (D) Changes in MFI are quantified in (E) The data shown are representative of three independent experiments (F) BJAB cells ... specific recruitment of FADD to internalized CD95 is, however, severely compromised in the presence of a caspase-8 inhibitor, even when accumulation of internalized CD95 is forced by treatment of...

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Báo cáo khoa học: Tec family kinases: Itk signaling and the development of NKT ab and cd T cells potx

Ngày tải lên : 28/03/2014, 22:21

... the conversion of negative to positive selection and a rescue of T-cell numbers in T cell receptor transgenic mice [6] More recently, Itk-deﬁcient mice were reported to have reduced development ... described as not being critical for the development of iNKT or cd T cells [69] However, given the more recent studies, a re-examination of the role of Ras in the development of these cells seems ... are similar in the fractions in the periphery [20] In the absence of Itk, there are reduced numbers of iNKT cells in the thymus and periphery [21] More detailed analysis of the development of...

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Báo cáo hóa học: "Involvement of aryl hydrocarbon receptor signaling in the development of small cell lung cancer induced by HPV E6/E7 oncoproteins" ppt

Ngày tải lên : 18/06/2014, 16:20

... are reported Interestingly, among all the genes deregulated by the E6 /E7 co-expression, 116 genes are associated to neurogenesis The list of these genes is reported in additional file These results ... esophageal cancer [29] and colorectal cancer [30] Furthermore the association between the presence of HPV 16 and the development of head and neck cancer has been recently established [31] The ... Among these, 289 genes were up-regulated and 272 were down-regulated Genes are listed in the additional file reporting for each gene, probe ID, the fold change, p-value, gene symbol, Gene Bank and...

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Báo cáo sinh học: "Wnt signaling and the developmental fate of lung cells" ppsx

Ngày tải lên : 06/08/2014, 18:21

... promoter • Cells of the early embryo are progressively segregated into different lineages and different fates: for example, the endodermal lineage derives from the innermost of the three layers of ... because we lack definitive markers for stem cells.” The findings also reveal something of the delicate interplay between the timing and nature of signaling in determining cell fate For example, ... metaplasia are progenitor or stem cells, or a more differentiated cell type Because of the way their experiment was set up, Hogan favors the notion that the aberrant cells detected in their transgenic...

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Tài liệu Báo cáo khoa học: What MAN1 does to the Smads TGFb/BMP signaling and the nuclear envelope Luiza Bengtsson pdf

Ngày tải lên : 19/02/2014, 02:20

... RNAi does not affect the early development of the embryos [37] At later stages however, the Xenopus embryos showed severe morphological anomalies: their right eyes were absent or poorly formed [14] ... with other INM proteins and the lamina and negatively inﬂuence regulation of gene expression [2] Could emerin be involved? At least in C elegans embryos, Ce-emerin seems to provide a backup mechanism ... several of them (LAP2b, emerin, lamin A) can regulate gene expression [1,9,11,12]; future studies will have to tell whether they it on orders coming from the plasma membrane Acknowledgements The...

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Báo cáo khoa học: "Protection of chicken against very virulent IBDV provided by in ovo priming with DNA vaccine and boosting with killed vaccine and the adjuvant effects of plasmid-encoded chicken interleukin-2 and interferon-g" doc

Ngày tải lên : 07/08/2014, 23:22

... expression of viral genes under the control of the CMV promoter It appeared that the effects of INF-γ on the immune response are likely to be dependent on the animal species, the types of combined ... chickens, but subcutaneous injection did not increase host immunity [21,25] Therefore, the effects of IL-2 on the immune response appear to be affected by the types and combined methods of vaccines ... this method, the appropriate expression of genes inserted into the plasmid vector is essential for the production of protective immunity in the embryos Chicken embryos in the late stage have an...

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Báo cáo y học: "Antigen receptor signaling in the rheumatic diseases" doc

Ngày tải lên : 09/08/2014, 01:22

... homeostasis and tolerance to self These antigen receptor- dependent events are likely relevant for the interpretation of disease pathogenesis in signaling mutants Mouse models An extensive mouse model ... tolerance broken in these mice? The answer to this question is exceedingly complex because many of these models influence cell lineages other than B cells Indeed, genetic deletion of lymphocytes in ... closer resemblance to these relatively rare clinical entities than to common polygenic rheumatic diseases such as RA and SLE (The reader is referred to an excellent recent review [33].) Nevertheless,...

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Báo cáo sinh học: " Plasmid-encoded NP73-102 modulates atrial natriuretic peptide receptor signaling and plays a critical role in inducing tolerogenic dendritic cells" pdf

Ngày tải lên : 14/08/2014, 19:22

... The levels of expression of each of the proteins in the transfected cells were approximately the same as shown by the second set of bands in each IP Precipitates were recovered and eluted proteins ... prepared and edited the manuscript RFL reviewed the manuscript SSM directed research, reviewed the manuscript All authors have read and approved the final manuscript Competing interests The authors ... incubated at 37°C (E) Flow cytometric analysis of phenotypic markers of transfected hmDCs Results shown are from one representative experiment of three repeats Zhang et al Genetic Vaccines and Therapy...

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Báo cáo khoa học: Structure, signaling mechanism and regulation of the natriuretic peptide receptor guanylate cyclase pptx

Ngày tải lên : 28/03/2014, 23:20

... respectively The intervening sequences, the transmembrane sequence and a linker region between the PKLD and the GCD are encoded by exons and 16, respectively ANP receptor ECD – biochemical and biophysical ... presence of high natriuretic peptide levels It needs to be acknowledged that, at present, the direct evidence for the proposed chloride control of the ANP–NPRA system is limited to the observation of ... the absence of crystal contacts and in solution conditions closer to those of the native environment The three-dimensional reconstructions of the apo-ECD dimer and the ANP–ECD complex revealed...

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Tài liệu Báo cáo khoa học: Osmosensing and signaling in the regulation of mammalian cell function docx

Ngày tải lên : 18/02/2014, 16:20

... early prerequisite for the execution of apoptotic programs [25] Signaling mechanisms upstream of apoptotic volume decrease depend on the cell type and stimulus under investigation and have been ... hepatocyte to the plasma membrane [39], which depends on a ROS-mediated tyrosine phosphorylation of the epidermal growth factor (EGF) -receptor, the association of CD95 with the EGF -receptor, and phosphorylation ... cells with some focus on the hepatocyte A more general treatize on osmosensing and osmosignaling is provided elsewhere [9] Osmosensing in mammalian cells The investigation of osmosensing processes...

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Tài liệu Báo cáo khoa học: Redox regulation of dimerization of the receptor proteintyrosine phosphatases RPTPa, LAR, RPTPl and CD45 pdf

Ngày tải lên : 18/02/2014, 17:20

... quaternary structure of the chimeras compared to native RPTPa, or to the presence of low amounts of monomers Nevertheless, there was a clear difference in accessibility between oxidized and reduced ... gel, blotted and immunostained with anti-HA IgG (12CA5) These experiments were repeated at least three times and representative blots are shown here disulﬁde bond The epitope tag in wild-type ... at the )5 position relative to the TyrTrpPromotif However, in general the tryptic cleavage sites were not conserved (supplementary Fig S1) Nevertheless, it is evident from this series of experiments...

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Báo cáo khoa học: E2A participates in a ﬁne control of pre-mature B-cell apoptosis mediated by B-cell receptor signaling via transcriptional regulation of survivin, IAP2 and caspase-8 genes pot

Ngày tải lên : 16/03/2014, 04:20

... (cell growth was slightly delayed thereafter), the depletion of E2 A slightly prevented apoptotic cell death even in the presence of PMA/ ionomycin To confirm these findings, we examined the effects ... gene was used as an internal control The numbers under the panels indicate the number of cycles used for PCR of E2 A deficiency on the amounts of proteins whose mRNA levels were altered as noted ... expression of the E2 A gene and dramatically suppressed that of the Aiolos gene in DT40, but in E2 A)/) had no effects or similar effects on the expression of other disrupted genes (Fig 3A) and genes...

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Báo cáo khoa học: Structure, expression and regulation of the cannabinoid receptor gene (CB1 ) in Huntington’s disease transgenic mice ppt

Ngày tải lên : 16/03/2014, 18:20

... generate the 5¢-end of these cDNAs as the adapter sequence was present in each cloned insert and the adapter RNA was added before the reverse transcriptase reaction Several expressed sequence ... mice, we ﬁrst conﬁrmed that the rate of decrease in steady-state CB1 mRNA was dependent on the length of the CAG trinucleotide (nt) repeat and relative expression of the HD gene We then determined ... produced from the transgene Therefore, the length of the polyglutamine tract encoded by the human HD transgene or relative expression of the transgene affects the rate of HD progression in these...

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Báo cáo y học: " Regulation of IκBα expression involves both NF-κB and the MAP kinase signaling pathways." ppt

Ngày tải lên : 11/08/2014, 08:21

... intestine was significantly higher in the LPS-treated male mice as compared with the female mice The difference could be due to the difference of the kinetics of luciferase induction between ... shown on the right of the images Red represents the highest photons/sec while blue represents the lowest photons/sec B Quantification of the luciferase signal from the abdominal region of the body ... of the body for male and female mice respectively Data are expressed as billion photons/second Nonparametric significance levels for the difference between treatment groups were determined by...

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Báo cáo y học: " The anti-inflammatory effects of the tellurium redox modulating compound, AS101, are associated with regulation of NFB signaling pathway and nitric oxide induction in macrophages" doc

Ngày tải lên : 11/08/2014, 08:22

... to the iNOS promoter Nevertheless these effects are exerted at different kinetics I At h, AS101 probably enters the nucleus and may interfere with the DNA-binding ability of the NFB complex resulting ... abundance of tellurium in the human body Over the last decade, there has been an increased appreciation for the role of redox chemistry in the regulation of biological systems Understanding the mechanism ... effect on the other families of serine-, aspartic and metalloproteases, in good agreement with the predictions of their unique Te(IV)-thiol chemistry Furthermore, the proteolytic activity of the inactivated...

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báo cáo khoa học: " Genetic and transformation studies reveal negative regulation of ERS1 ethylene receptor signaling in Arabidopsis" pot

Ngày tải lên : 12/08/2014, 03:21

... of the single receptor genes ETR1 and ERS1, we characterized the ethylene response phenotype in (ETR1)ers1 etr2 ein4 ers2 and (ERS1)etr1 etr2 ein4 ers2 Effects of the respective loss of ETR1 and ... elusive The inhibitory effects of ERS1 on ethylene receptor signaling are unlikely to be due to its lack of the receiver domain, because ERS2 does not have the same effects as ERS1 Interestingly, etr1-7 ... imply one emergent function for ETR1 and ERS1 Positive effects of ERS1 on ethylene responses only occur in the presence of ETR1, implying another emergent function of ETR1 and ERS1 Ethylene receptor...

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Báo cáo y học: "Human receptor kinetics and lung tissue retention of the enhanced-affinity glucocorticoid fluticasone furoate" doc

Ngày tải lên : 12/08/2014, 15:21

... fluticasone propionate (FP) and mometasone furoate (MF) in human lung tissue The columns represent the mean and mean deviation of the mean from four independent experiments The left columns represent the ... protein Kinetics of receptor binding of glucocortiocids The receptor binding experiments were performed according to the procedure described earlier [1] based on [7-9] A Determination of receptor ... in the presence in human lung tissue was monitored over a period of 24 hours at an incubation temperature of 37°C (Figure 3) The incubations were performed in the presence and absence of the esterase...

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Báo cáo y học: "Parenteral versus enteral nutrition: effect on serum cytokines and the hepatic expression of mRNA of suppressor of cytokine signaling proteins, insulin-like growth factor-1 and the growth hormone receptor in rodent sepsis" pps

Ngày tải lên : 13/08/2014, 08:20

... which further influence the response to nutrients themselves Although our results may be heavily influenced by the design of the experiment and the experimental model, the complex interactions ... observed, any mechanistic influence of these on SOCS expression cannot be determined from the present study We recognize that there may be problems with interpretation of the results of the present study ... circulating levels of cytokines can explain these differences, but it seems unlikely that these effects are modulated via changes in number of GHRs While differences in cytokine levels were observed,...

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Báo cáo y học: "Genome-wide patterns of carbon and nitrogen regulation of gene expression validate the combined carbon and nitrogen (CN)-signaling hypothesis in plants" pptx

Ngày tải lên : 14/08/2014, 14:21

... which the regulation due to one cis element requires the presence of another [30] In the example examined here, the nitrogendependent cis element enhances the induction caused by the C-element, making ... genes whose promoters contain the C-element were identified The promoters of these genes were then checked for a second motif The number of genes that contained the C-element being analyzed and ... were tested to determine if any are NDEs by determining whether they are overrepresented in combination with a C-element within the promoters of the Model CN-enhanced genes Seven of the potential...

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