micro and nanoscale organizations of proteins modulate cell extracellular matrix interactions lessons for the design of biomaterials

Báo cáo khoa học: 15 N-Labelled proteins by cell-free protein synthesis Strategies for high-throughput NMR studies of proteins and protein–ligand complexes doc

Ngày tải lên : 07/03/2014, 12:20

... (e) The reaction mixture is accessible This allows the synthesis of proteins in the presence of other proteins provided in excess at the start of or during the reaction, e.g., for the purpose of ... spectrum which is the same as for a uniformly [15N]-labelled sample, the need to quantify cross-peak intensities, and the fact that the sequence speciﬁc N-labelled proteins by cell- free synthesis assignments ... general, proteins that can be produced in high yields in vivo are also suitable for efﬁcient production by cell- free synthesis In order to compensate for the increased effort and expense required for...

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biomechanics at micro- and nanoscale levels, v.iii, 2007, p.182

Ngày tải lên : 04/06/2014, 14:19

... image of the edge of the cell was positioned near the edge of the slit The slit was rotated, based on the orientation of the cell The photodiode system had a cutoff (3-dB) frequency of 1,200 Hz The ... (#1), then responded for a second time through cells #6 and #9 The magnitudes of the second calcium transient of cells #3 and #5 were similar to the magnitudes of the first responses (Fig 7), and ... behavior of bone cells in these networks modulate the signaling between osteocytic networks and osteoblasts on the surface of the bone The mean signal transmission speeds of 3.7±2.8 for 50 µm and...

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biomechanics at micro- and nanoscale levels, v.iv, 2007, p.181

Ngày tải lên : 04/06/2014, 14:42

... tensile testing After the tensile test, the distance between the micropipette and microplate L, the deflection of each microplate X, and the diameter of the cell perpendicular to the 16 T Matsumoto ... detected in the lane of WT prestin Two distinct bands of 100 kDa and 80 kDa were observed in the lane of G127A In the lanes of T128A, S129A and R130A, weak 100 kDa bands and 80 kDa and 70 kDa bands ... observed in the P-fracture face of the lateral membrane of the OHC by electron microscopy [8] and those of the particles which were observed in the cytoplasmic face of the lateral membrane of the OHC...

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Gene targeting in human pluripotent cell derived neural stem cells for the study and treatment of neurological disorders

Ngày tải lên : 26/11/2015, 09:54

... Stem cells Stem cells have the remarkable potential to differentiate into specialized cells and thereby are key factors for the development of the whole organism All types of stem cells share their ... renders them unsuitable for genetic modifications However, the use of patient-derived stem cell populations offers an unlimited source of cells and the potential to derive the cell type of interest ... divide, forming after several divisions the blastocyst The outer layer consists of the trophoblast, giving rise to the placenta, while the inner cell mass forms all three germ layers of the embryo The...

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Tài liệu Báo cáo khoa học: Hypoxia reduces the expression of heme oxygenase-2 in various types of human cell lines A possible strategy for the maintenance of intracellular heme level pdf

Ngày tải lên : 19/02/2014, 06:20

... decreased the expression levels of HO-2 mRNA and protein in Jurkat T-lymphocytes and YN-1 and K562 erythroid cell lines To further analyze the effects of hypoxia on the expression of HO-1 and HO-2, ... respect to the intensity for the a-tubulin signal Shown are the relative expression levels of HO-1 and HO-2 proteins in YN-1 cells (B) and those of HO-2 protein in K562 cells (D) The intensity ... HepG2 cells (Fig 2B) Taken together, these results indicate that hypoxia reduces the expression levels of HO-2 mRNA and protein in ﬁve out of six cell lines, with the exception of KG1 myeloid cells...

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Báo cáo khoa học: The structural comparison of the bacterial PepX and human DPP-IV reveals sites for the design of inhibitors of PepX activity pot

Ngày tải lên : 23/03/2014, 13:20

... lactis The results are presented in Table and Fig 4A For the cluster of lowest energy, both the position and the conformation of the ligand are close to those observed in the 3D structure of the ... group of the ligand and the NH2 of Asn470 (equivalent to Asn710 in DPP-IV) Another potential bond can be proposed between the OH atom of Tyr380 and the carbonyl group of the ligand Finally, the ... comparison of the active and speciﬁcity sites of the two X-PDAP enzymes, the bacterial PepX (green) and the human DPP-IV (orange) (B) Superposition of the active and speciﬁcity sites of the S9 enzymes...

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API - 2510A - Fire-Protection Considerations for the Design and Operation of Liquefied Petroleum Gas (LPG) Storage Facilities

Ngày tải lên : 27/03/2014, 14:07

... ascertain the following basic information for all LPG Þres: a The time of the ÞreÕs inception if there is ßame impingement or engulfment of the vessel b The liquid level in the storage vessel and whether ... vessel and the center of the pool Þre at which cooling water should be applied d As an example, if the diameter of the propane pool Þre is 20 feet, the distance between the vessel and the center of ... be used if the design of the storage vessel, piping, and spill containment incorporates the Þre prevention features in Section If the design increases the risk of full engulfment of the vessel...

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Báo cáo hóa học: "Research Article WING/WORLD: An Open Experimental Toolkit for the Design and Deployment of IEEE " ppt

Ngày tải lên : 21/06/2014, 23:20

... gateway for the mesh network The latter action disables the current backhaul link and use the multihop wireless backhaul as default route to the Internet The performances of the self-configuring and ... estimate of the highest effective throughput achievable in the forward direction, and PACK is the delivery probability of the ACK signal in the reverse direction (drev ) Since rx is the estimated ... lightweight loads, and the limited radio capabilities, due to small antennas and low power WiFi cards On the other hand, embedded platforms provide high flexibility in terms of custom and off -the- shelf...

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báo cáo khoa học: " NorthStar, a support tool for the design and evaluation of quality improvement interventions in healthcare" ppt

Ngày tải lên : 11/08/2014, 05:22

... tailor the intervention (Designing the Intervention) NorthStar assists the user in the choice of appropriate study design to test the QI intervention (Choosing the Study Design) It then discusses the ... interventions and their evaluation The intention was for the tool to be useful to users with varying levels of experience in QI research, and for the text to be instructional as well as informative For ... of practice" in designing the intervention and on how to design a cluster RCT as the preferred evaluation study design Determinants of practice are the factors that affect practice patterns and...

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Báo cáo y học: " Serodiagnosis of sheeppox and goatpox using an indirect ELISA based on synthetic peptide targeting for the major antigen P32" ppt

Ngày tải lên : 12/08/2014, 01:22

... carried out the MNT and established indirect ELISA YS participated in the design of the study and performed the statistical analysis XL conceived of the study, and participated in its design and coordination ... cut-off value of 0.2, determined from a mean P/N of 1.516 with a SD of 0.056 for the 10 negative sera The specificity and sensitivity of the assay at three different cut-off values, 0.1, 0.2 and ... Page of dilutions were 1:10 for serum and 1:2000 for anti-goat HRP-IgG Exposure time was optimally 45 for serum samples and 15 for conjugate at 37°C Table The intra- and inter-assay coefficient of...

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Báo cáo y học: " Gene expression profiling of human prostate cancer stem cells reveals a pro-inflammatory phenotype and the importance of extracellular matrix interactions" pps

Ngày tải lên : 14/08/2014, 08:21

... components of the extracellular matrix- receptor system and 15 components of the focal adhesion signaling pathway It is worth noting that five members of the focal adhesion pathway and the extracellular ... data analysis and design SL performed the stem cell isolations from benign samples PB performed stem cell isolations from tumors CH performed culturing and isolation of stem cells, and NF-κB experiments ... against the consensus sequence of the known transcripts for the target gene Microarrays carry multiple probes against the same gene distributed throughout the length of the transcript, some of which...

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guide for the design and construction of fixed offshore concrete structures

Ngày tải lên : 24/10/2014, 17:40

... accordance with the results of the sea floor survey, considering the deviations from a plane surface, the force-deformation properties of the soil, and the geometry of the base of the structure The possibility ... indicated for consideration by the designer The design of offshore structures requires much creativity of the designer, and it is intended that this guide permit and encourage creativity and usage of ... Stability of the sea floor 5.2.1 Slope stability- The stability of the sea floor in the vicinity of the structure should be investigated The study should include the effects of the structure on the...

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guide for the design and construction of concrete reinforced with frp bars

Ngày tải lên : 24/10/2014, 21:59

... between the bar and the surrounding concrete The stress at the surface of the bar resulting from the force component in the direction of the bar can be considered the bond stress between the bar and ... ensure yielding of steel before the crushing of concrete The yielding of the steel provides ductility and a warning of failure of the member The nonductile behavior of FRP reinforcement necessitates ... transportation costs and may ease handling of the bars on the project site 3.1.2 Coefficient of thermal expansion The coefficients of thermal expansion of FRP bars vary in the longitudinal and transverse...

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guide for the design and construction of externally bonded frp systems for strengthening concrete structures

Ngày tải lên : 24/10/2014, 21:59

... orientation of the fibers, and the quantity of fibers primarily govern the tensile properties of the FRP material Due to the primary role of the fibers and methods of application, the properties of an ... Composite Fiber volume fraction The ratio of the volume of fibers to the volume of the composite Fiber weight fraction The ratio of the weight of fibers to the weight of the composite Filament—See ... as to develop the strength of the FRP system The development length is a function of the strength of the substrate and the rigidity of the bonded FRP Durability, FRP The ability of a material...

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guide for the design, construction, (reapproved 1999) and repair of ferrocement

Ngày tải lên : 24/10/2014, 22:11

... state of the art The procedure used on a particular project should be based on the experience and the ingenuity of the builders and the judgment of the engineer DESIGN, CONSTRUCTION, AND REPAIR OF ... placing the mortar The chemical composition of the cement, the nature of the aggregate, the aggregate-cement ratio, and the watercement ratio are the major parameters governing the properties of the ... fraction, the specific surface of reinforcement, and the effective modulus of the reinforcement sections, pg 549.1R-25 2.1.1 Volume fraction of reinforcement Vf-Vf is the total volume of reinforcement...

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Development and implementation of efficient segmentation algorithm for the design of antennas and arrays

Ngày tải lên : 11/09/2015, 09:02

... CONDITION NUMBER OF THE BANDPASS FILTER VERSUS THE MATRIX STAGES 79 FIG.3.15: SPECTRAL RADIUS OF THE BANDPASS FILTER VERSUS THE MATRIX STAGES 79 FIG.3.16: REFLECTION COEFFICIENTS OF THE BANDPASS FILTER ... scheme for the Green’s function for fast evaluation of the MoM matrix elements and the computation of the radiation patterns Finally, a patch antenna is analyzed to demonstrate the accuracy of the ... BETWEEN THE SPECIFICATIONS AND THE MEASUREMENTS OF THE BANDPASS FILTER 82 TABLE 3.6: SPECIFICATIONS OF THE SERIES-FED ARRAY 84 TABLE 3.7: COMPARISON OF THE RELATIVE ERROR AND THE...

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Working PaPer SerieS no 1150 / January 2010: Do bank loanS anD creDit StanDarDS have an effect on outPut? a Panel aPProach for the euro area doc

Ngày tải lên : 15/03/2014, 10:20

... countries only entered the euro area towards the end of the sample and therefore in the earlier part of the sample were not exposed to the single monetary policy to the same degree as the original euro ... in the euro area These …ndings continue to hold even when we control for the impact of the stance of bank credit standards on lending and activity Overall, the …ndings of this paper highlight the ... substituted with other forms of funding; a particularly realistic hypothesis at the current juncture The second assumption regards the peculiarity of loans for …rms (and households), in the sense that...

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Recruitment and Retention - Lessons for the New Orleans Police Department docx

Ngày tải lên : 15/03/2014, 20:20

... Like the rest of the citizens, many of the police lacked the basics of life: food, clean water, and facilities for personal sanitation For many of the citizens of New Orleans, the aftermath of the ... recruiting; the mix of oﬃcers and civilians; and ways to improve the morale of the NOPD For each topic, we discuss the problem facing the NOPD and the lessons RAND has learned from previous studies and ... departure from the force and an inability to recruit new oﬃcers The Superintendent of Police of the City of New Orleans asked the RAND Corporation for help in addressing the recruiting and retention...

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Recent Large Service Acquisitions in the Department of Defense - Lessons for the Office of the Secretary of Defense pot

Ngày tải lên : 23/03/2014, 01:20

... review to ensure high standards for research quality and objectivity Recent Large Service Acquisitions in the Department of Defense Lessons for the Office of the Secretary of Defense FRANK CAMM ... Supported by the Office of the Secretary of Defense Approved for public release, distribution unlimited The research described in this report was sponsored by the Office of the Secretary of Defense ... additional information and to expand the number of acquisitions covered in the study to six to test the robustness of the issues identified in the December briefing In March 2002, OSD also asked RAND...

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Child health nurses in the Solomon Islands: lessons for the Pacific and other developing countries docx

Ngày tải lên : 24/03/2014, 00:20

... panic.’ Other comments related to the structure of the course and rotation between theoretical units and the university and clinical components undertaken in both the hospital and the community ‘There ... community ‘There was theory and clinical practice We learned the theory and then we did the clinical practice and the competencies for assessment as well as theory exams There was a lot of clinical practice, ... to remote and very remote regions of their provinces Nurses’ perceptions of their roles A common theme was a feeling of being overwhelmed by the scope of their role without the support of similarly...

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