biomechanics at micro- and nanoscale levels, v.iii, 2007, p.182

[...]... Voltage-dependent Outer Hair Cell Motility and Capacitance J Neurosci 11, 3096-3110 32 Armstrong, C.M., Bezanilla, F., 1974 Charge movement associated with the opening and closing of the activation gates of the Na channels J Gen Physiol 63, 533-552 33 Kakehata, S., Santos-Sacchi, J., 1996 Effects of salicylate and lanthanides on outer hair cell motility and associated gating charge J Neurosci 16, 48814889... substrate, and a grid of adhesive ECM islands and lines limits cell attachment to those islands Although other modified silanes have been used to pattern bone cells [18, 24] into relatively thick lines or islands (several cells wide), alkanethiol SAMs have not been previously used to pattern bone cells, and bone cells have never been cultured in network patterns that closely mimic osteocyte networks in... create bone cell networks, 2) examine calcium wave propagation from a single bone cell indented using an atomic force microscope (AFM) to neighboring cells in this bone cell network, and 3) examine the effect of separation distance on calcium signal propagation 2 Materials and Methods 2.1 Microcontact printing for the formation of controlled bone cell networks Fibronectin (FN) patterns were created... on an IBM-compatible computer and a 16-bit A/D converter (Digidata 1322A, Axon Instruments) 3 Results 3.1 Extracellular application of streptomycin and gentamicin OHC motility was measured from isolated cells before and after streptomycin and gentamicin were applied to the extracellular solution through a puffer pipette positioned 60 µm away from the cells The cells were held at -70 mV and voltage steps... spontaneously form ordered aggregates on metal-coated surfaces (e.g., gold, platinum), and SAM modified surfaces allow strict control of cell-surface interactions through the creation of micropatterns of ECM proteins, surrounded by non-adhesive SAM regions such that individual cells will attach and spread only to the ECM patterned adhesive regions The micropatterning of SAMs can be accomplished either... adsorb proteins and promote cell adhesion, while SAMs that present ethylene glycol moieties such as tris-(ethylene glycol)-terminated alkanethiols (HS-(CH2)11(OCH2CH2)3OH) effectively resist protein absorption and cell adhesion [15, 17, 18, 20-23] Thus, the patterning of these two SAMs on a substrate defines the pattern of ECM proteins that are adsorbed from solution onto the substrate, and a grid of... examples of two OHCs before and 2-minutes after 100 µM streptomycin and gentamicin were applied The motile response was asymmetric, with contraction being larger than the elongation The response was also nonlinear, with saturation at both directions We measured a total of 10 cells (5 cells each) for streptomycin and gentamicin at the concentration of 100 µM Streptomycin and gentamicin did not change... was ruptured, and at 30 and 200 seconds after the cell was ruptured Steady-state responses (peak-to-peak) at different moments during perfusion were measured and plotted in the bottom panel The Effect of Streptomycin and Gentamicin on Outer Hair Cell Motility 9 was observable immediately after the cell was ruptured We measured the magnitude of motility at different times during perfusion and the magnitude... (CS-FBS; Hyclone Laboratories Inc., Logan, UT) and allowed to migrate onto patterns for 24 hours 2.2 Optimization of geometric parameters for bone cell network formation To confirm good micropatterning, coverslips patterned with FN but not seeded with cells were subjected to immunofluorescence staining for FN using an anti-FN primary antibody (Chemicon, Temecula, CA) and a FITC conjugated secondary antibody... fabrication of the stamp features, the nominal features of stamps with a fixed 50 µm separation and variable line widths and circle diameters were measured 3 times and averaged from images obtained using a light microscope and Scion Image (Frederick, MD), image analysis software Mechanotransduction in Bone Cell Networks 17 To determine the most optimal line widths and circle diameter sizes for the formation . Street, Covent Garden, London WC2H 9HE Printed in Singapore. BIOMECHANICS AT MICRO- AND NANOSCALE LEVELS Volume III v PREFACE A project on Biomechanics at Micro- and Nanoscale Levels, . alt="" BIOMECHANICS AT MICRO- AND NANOSCALE LEVELS VOLUMEII Biomechanics at Micro- and Nanoscale Levels Editor-in-Charge: Hiroshi Wada (Tohoku University, Sendai, Japan) Published Vol. I: Biomechanics. Biomechanics at micro- and nanoscale levels 1. Cell mechanics 2. Molecular biomechanics 3. Mechanobiology at micro- and nanoscale levels 4. Computational biomechanics We are planning to publish