kinase glycogen synthase kinase and mitogen activated protein kinase signalling cascades in neuronal cell survival what the neurotrophins have taught us and implications for neuropsychiatric disorders

Tài liệu Báo cáo khoa học: The Arabidopsis protein kinase Pto-interacting 1-4 is a common target of the oxidative signal-inducible 1 and mitogen-activated protein kinases docx

Ngày tải lên : 14/02/2014, 19:20

... of the input was loaded on to the gel and represents the amount of HIS-tagged proteins used for the assay The in vitro binding assays were repeated twice using recombinant proteins prepared independently ... subdomains VII and VIII [8,9] Such as OXI, other AGC kinases of the AGC VIII subgroup have been shown to be involved in various signalling pathways, including blue light signalling [10] and auxin signalling ... group [8,9] The AGC kinases were named on the basis of their homology to the mammalian cAMP-dependent protein kinase A, cGMP-dependent protein kinase G and phospholipid-dependent protein kinase C...

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Báo cáo khoa học: Adenine nucleotides inhibit proliferation of the human lung adenocarcinoma cell line LXF-289 by activation of nuclear factor jB1 and mitogen-activated protein kinase pathways doc

Ngày tải lên : 07/03/2014, 12:20

... of the inhibitors for the kinases calcium ⁄ calmodulin-dependent protein kinase (CaMKII), extracellular signal-regulated kinase ⁄ (ERK1 ⁄ 2), p38 mitogen- activated protein kinase (MAPK), protein ... coordinated signaling involving the p38 mitogen- activated protein kinase (MAPK) pathway and caspase activation [18] Several mechanisms have been found for the inhibitory action of ATP on cancer cell ... This is indicated by the massive increase of cells in the S phase, the reduction in cell proliferation, and the decrease in bromodeoxyuridine (BrdU) incorporation into newly synthesized DNA In addition,...

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Cyclic nucleotides and mitogen-activated protein kinases: regulation of simvastatin in platelet activation pptx

Ngày tải lên : 10/08/2014, 05:21

... suggesting that the binding of fibrinogen to the αIIbβ3 integrin is the final common pathway for agonistinduced platelet aggregation Therefore, we further evaluated whether or not simvastatin directly ... approved by the Institutional Review Board of Taipei Medical University and conformed to the principles outlined in the Helsinki Declaration, and all human volunteers provided informed consent In brief, ... Simvastatin (30 and 50 μM) did not significantly affect FITC-triflavin binding to the αIIbβ3 integrin in platelet suspensions (c, 4.8 ± 0.1; d, 4.9 ± 0.1) (Fig 1C), indicating that the inhibitory...

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Báo cáo y học: "A3 adenosine receptors and mitogen-activated protein kinases in lung injury following in vivo reperfusion" pps

Ngày tải lên : 12/08/2014, 23:23

... fied: the extracellular signal-regulated kinases (ERKs), the cJun amino-terminal protein kinases (JNKs) and the p38 kinases MAPKs have been shown to be activated in animal models of reperfusion injury ... Activation of mitogen- activated protein kinases and activator protein- 1 in myocardial infarction in rats Cardiovasc Res 1998, 38:116-124 Chang L, Karin M: Mammalian MAP kinase signalling cascades ... stress -activated mitogen- activated protein kinases and c-jun N-terminal kinases are activated by ischemia/reperfusion Circ Res 1996, 79:162-173 Abe J, Baines CP, Berk BC: Role of mitogen- activated protein...

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Báo cáo khoa học: Dematin interacts with the Ras-guanine nucleotide exchange factor Ras-GRF2 and modulates mitogen-activated protein kinase pathways doc

Ngày tải lên : 17/03/2014, 11:20

... membrane-associated proteins to the actin cytoskeleton Due to the abundant expression of dematin in the brain, we searched for dematin-interacting proteins by screening a human brain cDNA library using the yeast ... Dematin and Ras-GRF2 associate in mouse brain lysate and in transfected epithelial cells To test whether Ras-GRF2 and dematin associate in vivo, we examined their association in mouse brain lysate ... binding interface and its stability within the core domain of dematin The inability of dematin to bind to the DH domain of human Ras-GRF1, as well as lack of binding between limatin (abLIM) and...

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Báo cáo Y học: Rum1, an inhibitor of cyclin-dependent kinase in ﬁssion yeast, is negatively regulated by mitogen-activated protein kinase-mediated phosphorylation at Ser and Thr residues pptx

Ngày tải lên : 17/03/2014, 23:20

... determines the cell- cycle timing of the G1–S transition (Start), maintaining cells in the pre-Start state until they have attained the minimal critical mass required to initiate the cell cycle In addition, ... sequentially activated protein kinases, namely, MAPK kinase kinase (MAP3K), MAPK kinase (MAP2K), and MAPK The extracellular signals are mediated intracellularly as an activation of MAP3K that further ... Cdc2 kinase inhibitor The p25rum1 protein is known to inhibit the kinase activity of a complex of Cdc2 and B type cyclin and thereby regulate the cell cycle of ﬁssion yeast Consistently, GST fusion...

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Báo cáo Y học: CTGF/Hcs24 induces chondrocyte differentiation through a p38 mitogen-activated protein kinase (p38MAPK), and proliferation through a p44/42 MAPK/extracellular-signal regulated kinase (ERK) doc

Ngày tải lên : 24/03/2014, 04:21

... (1999) Mitogenactivated protein (MAP) kinases are involved in interleukin-1 (IL-1)-induced IL-6 synthesis in osteoblasts: modulation not of p38 MAP kinase, but of p42/p44 MAP kinase by IL-1 -activated ... MEK and MKK isoforms Science 267, 682 –685 Enslen, H., Raineaud, J & Davis, R.J (1998) Selective activation of p38 mitogen- activated protein (MAP) kinase isoforms by the MAP kinase kinase MKK3 and ... ERK and p38 MAP kinase in HCS-2/8 cells We analyzed the effects of rCTGF/Hcs24 on the phosphorylation of two major types of MAP kinases, ERK and p38 MAP kinase, by Western blotting in HCS-2/8 cells...

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Báo cáo y học: "Strong inhibition of TNF-α production and inhibition of IL-8 and COX-2 mRNA expression in monocyte-derived macrophages by RWJ 67657, a p38 mitogen-activated protein kinase (MAPK) inhibitor" ppt

Ngày tải lên : 09/08/2014, 01:23

... in endothelial cells and in the lining layer [10] Inhibition of p38 MAPK therefore could provide an interesting target for intervention in inflammation, as it occurs in the synovia in RA In vitro ... N-terminal kinase, and p38 mitogen- activated protein kinase, in synovial tissue and cells in rheumatoid arthritis Arthritis Rheum 2000, 43:2501-2512 Ebringer A, Wilson C: HLA molecules, bacteria and ... are rare, and these drugs have side effects [7,8] The importance of mitogen- activated protein kinases (MAPKs) in cell biology has been reported in many studies concerning different inflammatory...

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Báo cáo y học: "CD95-induced osteoarthritic chondrocyte apoptosis and necrosis: dependency on p38 mitogen-activated protein kinase" ppsx

Ngày tải lên : 09/08/2014, 07:20

... by increasing cell proliferation in addition to decreasing cell death Our observations in vitro using OA chondrocytes have strong implications for our understanding of the mechanisms underlying ... ± standard deviation Results Expression of CD95 and CD95 ligand To determine whether CD95 and its ligand were involved in induction of cell death in cartilage, we examined their expression in ... & Therapy Vol No Wei et al p38 Mitogen- activated protein kinase (MAPK) belongs to a family of stress kinases that are activated by proinflammatory cytokines and environmental stresses including...

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Báo cáo y học: "CD95-induced osteoarthritic chondrocyte apoptosis and necrosis: dependency on p38 mitogen-activated protein kinase" pps

Ngày tải lên : 09/08/2014, 07:20

... and findings on conventional MRI Nine patients tested positive for IgM aCLs, nine for IgG aCLs and 13 for Lac, yielding a comparison of nine versus nine patients for IgM aCL, nine versus nine ... aCLs, in addition to contributing to overt brain infarcts, may also contribute to widespread microscopic damage in the brain Competing interests The authors declare that they have no competing interests ... [26-31], spin-spin relaxation time measurements [32] and diffusion-weighted imaging [33] A recent study combining these MRI techniques [10] indicated that the presence of neuronal and axonal injury,...

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Báo cáo y học: "The S100A8/A9 heterodimer amplifies proinflammatory cytokine production by macrophages via activation of nuclear factor kappa B and p38 mitogen-activated protein kinase in rheumatoid arthritis" potx

Ngày tải lên : 09/08/2014, 07:20

... the lining layer However, S100A8/A9 staining was negligible in CD68-negative cells in the sublining layer, including lymphocytes and vascular endothelial cells Immunostaining of the S100A8 and ... ampicillin for 24 hours; the cells produced the proteins as inclusion bodies The harvested cells were solubilized with BPER™ Bacterial Protein Extraction Reagent (Pierce, Rockford, IL, USA) The inclusion ... (TNF-α) and interleukin (IL-1) [15] These two cytokines, produced at high levels by macrophages localized in the lining layer and at the pannus lesion, induce the synthesis of numerous inflammatory...

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Tài liệu Báo cáo khoa học: Mitogen-activated protein kinase phosphatase-1 modulated JNK activation is critical for apoptosis induced by inhibitor of epidermal growth factor receptor-tyrosine kinase pdf

Ngày tải lên : 18/02/2014, 13:20

... on them for their survival and, consequently, undergo apoptosis following inhibition of EGFR tyrosine kinase by geﬁtinib Geﬁtinib has been shown to inhibit cell survival and growth signaling ... selective protein kinase C inhibitor, Ro-31-8220, inhibits mitogen- activated protein kinase phosphatase-1 (MKP1) expression, induces c-Jun expression, and activates Jun N-terminal kinase J Biol ... responsible for the AG1478-induced apoptosis in PC-9 cells Stress stimuli that induce apoptosis, including UV- and c-irradiation, heat shock, protein synthesis inhibitors, DNA-damaging agents and the...

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Tài liệu Báo cáo khoa học: Hypoxia downregulates farnesoid X receptor via a hypoxia-inducible factor-independent but p38 mitogen-activated protein kinase-dependent pathway doc

Ngày tải lên : 18/02/2014, 13:20

... (2000) The pyridinyl imidazole inhibitor SB203580 blocks phosphoinositide-dependent protein kinase activity, protein kinase B phosphorylation, and retinoblastoma hyperphosphorylation in interleukin-2-stimulated ... promoting binding of the von Hippel–Lindau tumor suppressor protein, which acts as the ubiquitin ligase for HIF-a proteins, leading to the degradation by the proteasome [20] Any HIF-a escaping this ... disorders by activating two signaling pathways: one is the HIF-dependent pathway that leads to the induction of VEGF, resulting in hypervascularity in the liver; and the other is the HIF-independent...

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Báo cáo khoa học: Identiﬁcation of mitogen-activated protein⁄extracellular signal-responsive kinase kinase 2 as a novel partner of the scaffolding protein human homolog of disc-large docx

Ngày tải lên : 22/03/2014, 16:20

... hDlg in ERK cascade O Maıga et al ¨ Introduction The mitogen- activated protein kinases (MAPKs) are a family of S ⁄ T -protein kinases, including p38, c-Jun N-terminal kinase and extracellular ... C-terminus of MEK2 fused to the GAL4 activating domain (C) The PDZ-1-2 domains of hDlg fused to the GAL4 DNA-binding domain were co-expressed in yeasts with the C-terminus of MEK2, encompassing the ... adenomatous polyposis coli, and the motor proteins kinesin and dynein, has also been previously highlighted in different cell types [19,21,22] In this context, hDlg controls intracellular trafﬁcking and...

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Báo cáo Y học: Atlantic salmon possess three mitogen activated protein kinase kinase 6 paralogs responding differently to stress pot

Ngày tải lên : 24/03/2014, 00:21

... mitogen- activated protein kinase kinase kinases in signal integration Oncogene 26, 3159–3171 Zarubin T & Han J (2005) Activation and signaling of the p38 MAP kinase pathway Cell Res 15, 11–18 ... Hansen et al cascade consisting of the MAPK kinase kinase (MAP3K), the MAPK kinase (MKK or MAP2K) and the MAPK The MAPKs are activated upon phosphorylation of Thr and Tyr in the activation loop by ... MAP kinase kinases by speciﬁc MAP kinase kinase kinases Mol Cell 18, 295–306 Winter-Vann AM & Johnson GL (2007) Integrated activation of MAP3Ks balances cell fate in response to stress J Cell...

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Báo cáo Y học: Mitogen-activated protein kinase kinase negatively modulates ciliary neurotrophic factor-activated choline acetyltransferase gene expression pdf

Ngày tải lên : 31/03/2014, 21:21

... MAP kinase kinase kinase, MAP kinase kinase and MAP kinase Curr Opin Genet Dev 4, 82±89 15 Hill, C.S & Treisman, R (1995) Transcriptional regulation by extracellular signals: mechanisms and speci®city ... to increase intracellular ACh levels via increasing ChAT protein levels Furthermore, even though the MAPK signaling is required for CNTF-mediated activation of certain genes in the CNS [26], in ... speci®c for JAK kinases in vitro and signi®cantly reduces the DNA binding activity of Stat3 in vivo [32] First, we tested the ability of the inhibitor to block the Jak2/Stat3 pathway in SN56T17 cells...

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Báo cáo y học: "Chondrocyte response to growth factors is modulated by p38 mitogen-activated protein kinase inhibition" pdf

Ngày tải lên : 09/08/2014, 01:23

... Q, Steiner G: Activation, differential localization, and regulation of the stress -activated protein kinases, extracellular signal-regulated kinase, c-JUN N-terminal kinase, and p38 mitogen- activated ... et al protein kinase activity, protein kinase B phosphorylation, and retinoblastoma hyperphosphorylation in IL-2-stimulated T cells independently of p38 mitogen- activated protein kinase J Biol ... human inducible nitric oxide synthase gene (AdiNOS) were used in some studies to facilitate evaluation of the effects of NO independent of other actions of iNOS inducing cytokines on the cell The...

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Báo cáo y học: "Octacalcium phosphate crystals directly stimulate expression of inducible nitric oxide synthase through p38 and JNK mitogen-activated protein kinases in articular chondrocytes" pps

Ngày tải lên : 09/08/2014, 06:23

... containing a serine/threonine protein kinase that interacted with the cytoplasmic domain of β1 integrin [51] Furthermore, Liu and Lioté [37] found that, within a few minutes, MSU crystals activated ... pepstatin, aprotinin and leupeptin) After sonication, the cells were incubated on ice for 15 and centrifuged at 14,000 rpm for 10 at 4°C The supernatants containing cell lysates were collected, and ... treated with various pharmacological inhibitors for 60 min, and then stimulated by various doses of OCP crystals or IL-1β For the indicated times in the cell cultures and days later in the cartilage...

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Báo cáo y học: "The p38 mitogen-activated protein kinase signaling cascade in CD4 T cells" docx

Ngày tải lên : 09/08/2014, 07:20

... protein kinase (MAPKAPK2 or MK2) [126] MAP kinase- activated protein kinase (MK3) [127] MAP kinase interaction protein kinase (MNK1) [128] p38 regulated /activated kinase (PRAK) [129] Mitogen- and ... After ligand binding, STAT proteins are recruited to the cytokine receptors and phosphorylated on tyrosine residues by Janus tyrosine kinases (Jaks) [76] Interestingly, in addition to tyrosine phosphorylation, ... LoGrasso P: Signalling for survival and death in neurones: the role of stress -activated kinases, JNK and p38 Cell Signal 2001, 13:299-310 Rincon M, Flavell RA, Davis RA: The JNK and p38 MAP kinase...

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Báo cáo y học: "Glucosamine affects intracellular signalling through inhibition of mitogen-activated protein kinase phosphorylation in human chondrocytes" pps

Ngày tải lên : 09/08/2014, 10:21

... amino-terminal kinase (JNK) and total JNK, (b) p-p38 and total p38, and (c) p-extracellular signal-regulated kinase (ERK)1/2 and total ERK1/2 were used to visualize mitogen- activated protein kinase ... interleukin-1 beta-stimulated matrix metalloproteinase-13 gene expression in human chondrocytes: suppression of c-Jun NH2-terminal kinase, p3 8mitogen- activated protein kinase and activating protein- I ... explaining the increased level of phosphorylation of ERK1/2 Nevertheless, glucosamine was ineffective in preventing phosphorylation of this kinase JNK and p38 are involved in cellular stress; they...

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