frap methods for visualizing protein dynamics in living mammalian cell nuclei

optimization of protein and rna detection methodologies and a new approach for manipulating protein activity in living cells

Ngày tải lên : 13/11/2014, 10:46

... tagged protein as wells as endogenous binding partners By sequestering protein complexes in the aggregates, activity is inhibited Finally, the detection of specific RNAs in living cells remains ... useful in other contexts 11 In certain cell types, a small fraction of the tetracysteine-GFP protein becomes palmitoylated, blocking biarsenical binding and targeting the fusion protein to the ... resuspended in HBSS and sorted into 15 ml tubes containing ml media containing 30% FBS Sorted cells were pelleted and cultured in media containing 0.5 mg / ml G418 Single cells were collected in 96-well...

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Báo cáo khoa học: "Methods for Using Textual Entailment in Open-Domain Question Answering" doc

Ngày tải lên : 08/03/2014, 02:21

... entailment information when identifying answers to questions Results from this baseline are presented in Table Creating New Sources of Training Data In order to obtain more training data for our ... System Processing textual entailment, or recognizing whether the information expressed in a text can be inferred from the information expressed in another text, can be performed in four ways We ... to the original question Integrating Textual Entailment in Question Answering In this section, we describe three different methods for integrating a textual entailment (TE) system into the architecture...

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Methods for solving inverse problems in mathematical physics prilepko, orlovskiy

Ngày tải lên : 17/03/2014, 14:30

... extensively investigated by other researchers including foreign ones Additional information in such problems is provided in the integral form and admits a physical interpretation as a result of measuring ... noting that the characteristics of the boundary being an equilibria in thermodynamics lead to supplementary problems for investigating inverse problems with the final overdetermination, since in ... discovering forces makingplanets movein accordance with Kepler’s laws was one of the first inverse problems in dynamics of mechanical systems solved in the past Inverse problems in potential theory in...

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Báo cáo Y học: Barrier passage and protein dynamics in enzymatically catalyzed reactions docx

Ngày tải lên : 31/03/2014, 23:20

... starting point for any dynamics study of a protein Second, there are a number of mutants of HLADH, which allow detailed study of the in uence of protein composition on protein dynamics, and how dynamics ... similar in this respect to the result obtained in Figs and In this review, we have described work pursued in our group over the past years demonstrating the potential for protein involvement in catalysis, ... intensity It is interesting to note that in locating the transition state location, deﬁned in this instance as the position of minimum coupling of the promoting vibration to the reaction coordinate, we...

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báo cáo hóa học:" Methods for depth-map filtering in view-plus-depth 3D video representation" pdf

Ngày tải lên : 21/06/2014, 17:20

... pixels, which affects in reducing phantom colors in the resulting image In our approach, we calculate filter weights using information from color frame in RGB, while applying filtering on depth map ... neighborhoods are formed for every pixel in the image domain X Once adaptive neighborhoods are found, one must find some modeling for depth channel before utilizing this structural information Constant ... modeling is simple, it has proven quite effective for mitigating the blocking artifacts arising from quantization of transform coefficients [14] In particular, it allows for establishing a direct link...

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Báo cáo y học: "Invasive and noninvasive methods for studying pulmonary function in mice" ppsx

Ngày tải lên : 12/08/2014, 15:21

... applying them in the mouse Unfortunately, at the present time, there is no gold standard for measuring lung function in mice, since none of the available methods is optimal in all regards Some investiga- ... Racila D, Elliott DE, Weinstock JV, Kline JN: Intestinal helminths protect in a murine model of asthma J Immunol 2006, 177:1628-1635 Bates JH, Mitzner W: Point : counterpoint lung impedance measurements ... such as methacholine (MCh) are administered to the animal at increasing doses either by aerosol inhalation or systemically by intravenous administration via the tail or jugular vein Airway responsiveness...

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Innovative methods for the Kannaviou CFRD in Cyprus pptx

Ngày tải lên : 13/08/2014, 22:21

... was followed by the reinforcing mats placing team These were transported down the face with the use of a trolley travelling on two rail lines The reinforcing mats were placed in the middle of the ... per cubic metre Reinforcing of 0.4% was adopted in each direction in the center of the slab The slabs were concreted using a 15m wide slip form The sealing of the perimeter joint consists of: ... Geotechnical Engineering such as: Rock and alluvial foundation grouting, diaphragm walls, rock anchoring, dam instrumentation and monitoring Planning and quality control of all the works involved in the...

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nondestructive test methods for evaluation of concrete in structures

Ngày tải lên : 24/10/2014, 17:44

... methods for determining structural properties and conditions Information derived from conducting these types of tests is important in evaluating the integrity of a concrete structure Such information ... pile shaft is indicated by change in slope of line representing arrival time of stress pulse as function of depth) radiometric methods and radiographic methods Both involve gaining information about ... additional information in Carino (1992) Fig 2.5.2 is a schematic of a continuous eddy-current covermeter In the absence of a reinforcing bar, the magnitude of the alternating current in the coil...

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Models and new methods for the quayside operations in port container terminals

Ngày tải lên : 10/09/2015, 15:48

... connection and routing within the surrounding area 1.2 Port Container Terminals and Quayside Operations In general terms, the container handling activities in a typical port container terminal can be ... container terminal Quay Crane Quayside Operation Yard Crane HIT Truck Vessel Discharging container flow Loading container flow Figure 1.2: Schema for container ow in a port container terminal ... indented berths for mega-containerships At rst, the authors rewrote the formulation of the problem proposed in Nishimura et al (2001), modifying the original non-linear integer programming formulation...

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Numerical methods for viscous fluid flows in sectors, cones, and domains with corners

Ngày tải lên : 11/09/2015, 09:05

... a cone, indicating that the observed eddy could be a natural way to allow for the streamlines of the flow with both in owing and outflowing zones to be enclosed within a finite container Forty years ... algebraically decaying boundary layer near the apex of a cone, there has been no direct evidence for the algebraically decaying boundary layers in inflowing flows in cones To summarize, in owing flows in cones ... domain is in nite in ζ To deal with the in nite domain we should either transfer the boundary conditions from in nity or introduce the basis functions for a spectral method defined in the in nite...

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A comparison of different regularization methods for a Cauchy problem in anisotropic heat conduction

Ngày tải lên : 16/06/2016, 01:11

... particular problems in order to make use of the maximum amount of information available The use of any extra information available for a specific problem is particularly important in choosing the regularization ... regularization methods developed for the Cauchy problems are considered in this paper These methods are investigated and compared in order to reveal their performance and limitation All the methods ... to transform the governing partial differential equation (1) into its canonical form by changing the spatial coordinates However, after the transformation, the domain deforms and rotates and the...

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báo cáo khoa học: "EGFR and COX-2 protein expression in non-small cell lung cancer and the correlation with clinical features" pptx

Ngày tải lên : 10/08/2014, 10:21

... that in squamous carcinoma (p = 0.022), which was consistent to previous findings of other study [21] This might provide basis for applying COX-2 inhibitor in adenocarinoma patients receiving ... JH, Wang LH, Cheng CM, Ciou SC, Lin ST, Jheng MY, Lin YW: Role of repair protein Rad51 in regulating the response to gefitinib in human non-small cell lung cancer cells Mol Cancer Ther 2008, 7(11):3632-3641 ... staining, the EGFR positive colon cancer section provided with the EGFR kit was used as positive control in each staining run For COX-2 staining, the positive control used the sample itself (internal...

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báo cáo khoa học: " Correction: EGFR and COX-2 protein expression in non-small cell lung cancer and the correlation with clinical features" pps

Ngày tải lên : 10/08/2014, 10:21

... Liao Dianying, Shen Yali, Xu Feng, Wang Jin: EGFR and COX-2 protein expression in non-small cell lung cancer and the correlation with clinical features Journal of Experimental & Clinical Cancer ... et al.: Correction: EGFR and COX-2 protein expression in non-small cell lung cancer and the correlation with clinical features Journal of Experimental & Clinical Cancer Research 2011 30:32 ... Center, West China Hospital, Sichuan University, PR China 2Department of Pathology, West China Hospital, Sichuan University, PR China Table (corrected table five) COX-2 expression in tumor and...

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Báo cáo y học: "Comparative analysis of processed ribosomal protein pseudogenes in four mammalian genomes" pot

Ngày tải lên : 14/08/2014, 21:20

... transporter protein is regulated by the expression of its pseudogene in the human genome [15] Thus, processed pseudogenes are emerging as interesting elements in the genomic landscape capable of being ... factors, including a 3% indel difference between the two species and the poorer quality of the chimpanzee genome Figure organism RP protein in one number and its corresponding ortholog in another ... pseudogenes in rodents are of recent origin This is in line with the reported increased retrotranspositional activity in rodents relative to humans and in accordance with research that indicates...

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Báo cáo khoa học: Analysis of the contribution of changes in mRNA stability to the changes in steady-state levels of cyclin mRNA in the mammalian cell cycle doc

Ngày tải lên : 30/03/2014, 20:20

... the cell cycle [11–15] Certain transacting proteins such as HuR were proposed as regulators of changes in mRNA stability during the cell cycle [15] In this context it is of interest that during ... in EL4 cells Cyclin E1 mRNA increased slightly in late G1 and then diminished about 2-fold in G2 ⁄ M phase The clearest induction in G2 ⁄ M compared to early G1 cells was observed for cyclin A2 ... speciﬁc mRNA-binding proteins interacting with AU-rich regions, notably HuR [15], it is conceivable that different cell lines express these proteins differently Alternatively, HuR might be induced...

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Tài liệu Báo cáo khoa học: Nop53p, an essential nucleolar protein that interacts with Nop17p and Nip7p, is required for pre-rRNA processing in Saccharomyces cerevisiae pdf

Ngày tải lên : 20/02/2014, 01:20

... Analyzing protein protein interactions using two-hybrid system Methods Enzymol 254, 241–263 Zanchin NIT & Goldfarb DS (1999) Nip7p interacts with Nop8p, an essential nucleolar protein required for ... [6,7] In this study, Nop53p was isolated in a two-hybrid screen as a protein interacting with Nop17p, which is involved in the early steps of prerRNA processing [28] Nop17p and Nop53p interacted in ... the interaction of Nop53p with other proteins (A) Test for positive interactions between Nop53p and other proteins fused to the Gal4p activation domain (AD), or to the lexA DNA binding domain...

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Tài liệu Báo cáo khoa học: "Generalization Methods for In-Domain and Cross-Domain Opinion Holder Extraction" pdf

Ngày tải lên : 22/02/2014, 02:20

... Christopher Manning 2005 Incorporating Non-local Information into Information Extraction Systems by Gibbs Sampling In Proceedings of the Annual Meeting of the Association for Computational Linguistics ... FICTION contains more out-ofdomain language Therefore, RB (which exclusively uses domain-independent knowledge) outperforms both learning-based methods including the ones incorporating generalization ... is, indeed, an improvement by adding information from RB resulting in a large boost in recall Already the application of our induction approach Induc results in an increase of more than 8% points...

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Báo cáo khoa học: Structural and functional roles for b-strand 7 in the a-crystallin domain of p26, a polydisperse small heat shock protein from Artemia franciscana pdf

Ngày tải lên : 07/03/2014, 12:20

... Siddique M & Vierling E (2001) The expanding family of Arabidopsis thaliana small heat stress proteins and a new family of proteins containing a-crystallin domains (Acd proteins) Cell Stress Chaperones ... a-crystallin domain residues and molecular modeling of protein structure The p26 a-crystallin domain contains nine b-strands arranged predominantly as paired b-sheets and possesses residues conserved in ... synthesized in mammalian and bacterial cells, and oligomerization is unaffected by protein puriﬁcation, observations important for subsequent analysis of the protein in in vitro assays Single-site...

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Báo cáo khoa học: "Experiments in Graph-based Semi-Supervised Learning Methods for Class-Instance Acquisition" docx

Ngày tải lên : 07/03/2014, 22:20

... reproducible research in this area Topics for future work include the incorporation of other kinds of semantic constraint for improved class-instance acquisition, further investigation into per-node ... class-instance acquisition performance can be signiﬁcantly improved by incorporating additional semantic constraints in the class-instance acquisition process, which for the experiments in this ... end up acquiring all the labels injected into the graph This is undesirable for two reasons: (1) for experiments involving a large numbers of classes (as in the previous section and in the general...

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Báo cáo khoa học: "Combining Stochastic and Rule-Based Methods for Disambiguation in Agglutinative Languages" pptx

Ngày tải lên : 08/03/2014, 05:21

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