... có tương quan với khối lượng thể theo phương trình : Prdt = a x W Trong a hệ số cho bảng : Bảng : Các hệ số tương ứng với trọng lượng thể Khối lượng lợn Hệ số a Khối lượng lợn (kg) Hệ số a (kg) ... Tổng số tiền chi cho thức ăn /số kg tăng trọng 3.7 Phương pháp xử lý số liệu Số liệu thu nhập xử lý phần mềm Excel xử lý thống kê theo phương pháp phân tích phương sai (ANOVA) qua mô hình tương quan ... hưởng đến chức gan tuyến tụy Nó với cystin để tạo lông, có tác dụng điều hoà trao đổi lipit, chống mỡ hoá gan, tham gia tạo nên serin, cystin Trong thể động vật, Methionine liênquan chặt chẽ đến...
... monitored using a Lambda ¨ spectrophotometer (PerkinElmer Instruments, Uberlingen, Germany) In order to quantify the precorrin-2, it was converted to sirohydrochlorin (e376 = 2.4 · 105 m)1Æcm)1 [35])...
... recombinant hMS is expressed as an apoenzyme in P pastoris at levels that enable purification of sufficient quantities for functional analysis (Table 1) A clear advantage of using Pichia as a heterologous ... 11 m formic acid and m HCl, and heated to 90 °C for 10 The acidification of the reaction mixture quantitatively converts CH3-H4-folate to CH+=H4-folate, which absorbs strongly at 350 nm (De = 26 ... was washed with · mL of water The combined radioactivity in the flowthrough and wash fractions was quantitated by scintillation counting For MSR-catalysed assays (used for measuring the Km of NADPH...
... Biochem Pharmacol 63, 381–391 44 Bradford M (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding Anal Biochem 72, ... methion43.19; H, 5.00; N, 18.31 C8H9N3Æ2HClÆO.2H2O requires: ine was eluted with mL of water and quantified using C, 42.95; H, 5.14; N, 18.78% a liquid scintillation counter (Packard Tricarb 1900CA; ... optimization Ligands were parameterized using partial atomic charges and other values obtained from quantum mechanic modelling (HartreeFock 6–31G*) of the ligand structure using pc spartan pro (Wavefunction...
... DNase I and reverse-transcribed into cDNA Quantitative analyses of CXCL12 and CXCR4 transcripts were performed by real-time PCR SYBR Green I system The quantity of transcripts was normalized with ... depleted DNMT1 Quantitative western blot showed 90% reduction of DNMT1 protein in Jurkat T cell line Total RNA was isolated, treated with DNase I and reverse-transcribed into cDNA Quantitative ... potential was observed by fluorescence microscopy and quantitative analysis Release of cytochrome C by mitochondria was determined using a semi-quantitative sandwich ELISA kit These results show,...
... that the presence of AMP in vivo does not have any quantitative consequence on the partition of the ux of Phser, at least under the physiological operating conditions dened in Table As a consequence, ... Table are unknown In order to describe the sensitivity of the system at the physiological operating point in quantitative terms, the results in Fig were used to calculate the ux response coefcients ... deaminase, respectively, coupled to lactate dehydrogenase Under these conditions, CGS and TS were operating in vitro at physiological concentration, with Phser concentration set by the system and...
... that the activities of these two enzymes in the three tumors (EAC, S180 and sarcoma tissue) were quantitatively more or less similar Arginase II activity was also quite high in these three tumors ... AGAT, GAMT, CT-1, arginase I and II, ODC, SAH hydrolase and methionine synthase expressions were quantified by RT-PCR 18S RNA was chosen as the housekeeping gene for normalization because its expression ... visualized and photographed by irradiating with UV light The band intensities were calculated with quantity one 1-D analysis software (BioRad, Hercules, CA, USA) Table Primers used for PCR amplification...
... crystallization experiments and unfolding studies was checked by SDS ⁄ PAGE and from absorbance ratios in the UV-vis spectrum; A525 ⁄ A280 ‡ 0.4 for ferric wt protein and A526 ⁄ A280 ‡ 0.4 for ... with an NMR pH electrode (Hamilton) All experiments were performed on a Bruker DMX600 spectrometer operating at a 1H frequency of 600.1 MHz and a temperature of 298 K 1D 1H spectra were acquired...
... Biochem J 301, 557–561 23 Bradford, M.M (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding Anal Biochem 72,...
... sample (20 lL) was eluted in the presence of M NaCl Polymalate in the eluate was hydrolysed and quantitated by the fluorimetric malate dehydrogenase assay An average amount of 0.069 ± 0.003 lg...
... The following supplementary material is available: Fig S1 Area quantitation of peaks obtained in polysomal profile analysis Fig S2 Quantitation of northern hybridizations Fig S3 Utp25p sequences ... from the same fractions were subjected to western blot for detection of ProtA and ProtA–Utp25p (C) Quantitation of the bands obtained from RNA co-ip by phosphorimaging Ratio of bound ⁄ input is shown ... EDTA Levels of 40S subunit are strongly decreased upon depletion of Utp25p Numbers indicate area quantitation of subunits peaks Based on the Utp25p nucleolar localization and its possible involvement...
... the presence of the dimer species (14% of the total protein analysed) The estimated frictional ratios for the monomeric species using the sedimentation coefficient distribution (c(s)), where f ... monomer; S, sedimentation coefficient; RH, radius of the structure; f ⁄ fo, estimated frictional ratios; ND, not determined; NA, not applicable R Experimental M (kDa)a M (kDa)b S020,W RHa (nm)...
... thí nghiệm, tỉ lệ sống, khối lượng cá xác định cách đếm cân toàn số cá bể Mẫu cá bể trữ lạnh nhiệt độ âm 20 oC để phân tích thành phần hóa học thể cá theo phương pháp AOAC (2000) Các giá trị trung ... tỉ lệ sống Kết cho thấy, tỉ lệ sống cá tra không ảnh hưởng mức methionine thức ăn Tuy nhiên, tỉ lệ sống thấp (76,7%) cá ăn thức ăn có hàm lượng methionine thấp (11,9 g/kg protein) Tỉ lệ sống cá ... động từ 76,7% đến 96,7% Kết nghiên cứu phù hợp với báo cáo Fagbenro (1998) cá trê (Clarias gariepinus); Coloso ctv (1999) cá chẽm (Lates calcarifer) tỉ lệ chết cá 304 liênquanđến hàm lượng...