biomarkers of oxidant stress in vivo oxidative modifications of lipids proteins and dna

Tài liệu The Early Detection Research Network: Investing in Translational Research on Biomarkers of Early Cancer and Cancer Risk docx

Ngày tải lên : 14/02/2014, 21:20

... lesions; clinical utility of certain prostate cancer biomarkers; developing and maintaining a registry of individuals harboring germline mutations for hereditary cancer syndromes; and identifying preneoplastic ... CT findings in the lung An extended set of genes is being investigated including APC, Cyclin D2, b-Catenin, FHIT, DNA Methyltransferase (DNA MTase), Calcitonin-Related Peptide and Deleted in Colorectal ... multidisciplinary, investigator-initiated projects with a strong administrative and data infrastructure In making cancer biomarkers of early detection and screening ready for large-scale clinical testing,...

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báo cáo hóa học:" Short term effects of milrinone on biomarkers of necrosis, apoptosis, and inflammation in patients with severe heart failure" pot

Ngày tải lên : 18/06/2014, 15:20

... continued to be at the discretion of the attending physician, including inotrope administration and dosing It is well recognized that heart failure leads to increased circulating levels of pro-inflammatory ... for ligand binding, therefore reducing apoptotic signaling Procedures All treatments including milrinone dosing was at the discretion of the patient's attending physician, with initial dosing between ... the setting of more routine decompensated heart failure, and the effect milrinone in ischemic subset of patients deserves further investigation Conclusion Initiation of milrinone therapy in patients...

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Báo cáo y học: " Biomarkers of systemic inflammation and depression and fatigue in moderate clinically stable COPD" pdf

Ngày tải lên : 12/08/2014, 13:22

... used to examine the categorical association of systemic biomarkers and depression and fatigue The multivariate linear and binary regression analyses were used to examine the association of factors ... in the median of the inflammatory biomarkers in quartiles of physical and cognitive Borg fatigue scores at baseline had a weak positive correlation with TNF-a and CRP (rho = 0.24, p = 0.01, and ... examined using the analysis of variance (ANOVA) The Mann-Whitney and Kruskal-Wallis tests were used to examine the difference in the median value of each biomarker in two groups or quartiles of...

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Báo cáo y học: " Restriction by APOBEC3 proteins of endogenous retroviruses with an extracellular life cycle: ex vivo effects and in vivo "traces" on the murine IAPE and human HERV-K elements" pot

Ngày tải lên : 13/08/2014, 05:21

... page) Murine and human APOBEC3 proteins inhibit endogenous retroviruses Murine and human APOBEC3 proteins inhibit endogenous retroviruses (A) Rationale of the assay for detection of infection ... APOBEC3 proteins might have actually impaired the in vivo amplification of these elements in the past, by searching for evidence of APOBEC3-editing on the endogenous copies residing in the murine and ... accumulation of inactivating mutations, some of them are still functional and have been cloned, thus allowing direct ex vivo assay of the effect of APOBEC proteins on their mobility Accordingly, several...

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Báo cáo y học: "Biomarkers of inflammation, coagulation and fibrinolysis predict mortality in acute lung injury" doc

Ngày tải lên : 13/08/2014, 08:21

... and fibrinolysis Specifically, we measured the inflammatory biomarkers ICAM-1, IL-6 and IL-8 and markers of disordered coagulation and fibrinolysis, including protein C, thrombomodulin and PAI-1 ... fibrinolysis in acute lung injury and acute respiratory distress syndrome Comparison of plasma levels of biomarkers of disordered coagulation and fibrinolysis in 50 patients with acute lung injury and ... is injurious in the acutely injured lung In a rat model of acid-induced lung injury, Frank and colleagues showed that lung endothelial and epithelial injury were minimized by a reduction in tidal...

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Báo cáo y học: "Plasma and synovial fluid microRNAs as potential biomarkers of rheumatoid arthritis and osteoarthritis" pot

Ngày tải lên : 12/08/2014, 12:20

... the function of miR-16 and miR132 in RA has not been determined yet, miR-16 is present in high levels in most of cells and thought to be potentially a master miRNA involved in determining mRNA stability ... 5'-CGT-CAC-CGG-GTG-TAA-ATC-AGCTTG-3' TA Cloning of PCR products and generation of standard curve To verify the PCR products and to generate standard curves of miRNAs, thymine adenine (TA) cloning was performed The ... phosphate-buffered saline (PBS) and cultured in ml of serum-free DMEM for 48 h Serum-free medium was used to exclude the contamination of miRNAs in bovine serum Page of 14 Synovial tissues of 30 mg were incubated...

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Tài liệu Báo cáo khoa học: Structure and function of active chromatin and DNase I hypersensitive sites pdf

Ngày tải lên : 14/02/2014, 18:20

... modiﬁcation and are maintained in a constitutively active state by CXXC domain proteins that bind to non-methylated CG sequences These proteins include MLL1 and the CG-binding protein Cfp1 that itself ... containing methyl binding domains (MBDs) which assemble complexes containing repressive HMT and DNMT proteins that act in a concerted fashion to maintain both DNA methylation and H3-K9 methylation ... complexes including co-factors such as CBP and SWI ⁄ SNF [85] In vivo footprinting conﬁrmed inducible binding of NFAT, AP-1, Sp1 and Runx1 [86,233] Nuclease digestion studies have determined that...

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Báo cáo khoa học: Functional classiﬁcation of scaffold proteins and related molecules pptx

Ngày tải lên : 06/03/2014, 22:21

... simple proteins binding two partners together (adaptors), larger multidomain proteins targeting and regulating more proteins in complex ways (scaffolds ⁄ anchoring proteins) and proteins specialized ... of their activity and subcellular localization, which is achieved through anchoring proteins, originally deﬁned as proteins targeting the action of protein kinase A (A-kinase anchoring proteins; ... phosphatase to myosin II [13] Docking proteins As outlined in the accompanying minireview by Brummer et al [6], docking proteins were originally deﬁned as accessory proteins in RTK signaling with a membrane-targeting...

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Interaction of EcoRI with noncognate DNA sequences computational investigation of dynamics of protein water and DNA conformation

Ngày tải lên : 09/09/2015, 18:49

... etc) and some bind to a class of DNA sequences (e.g., regulatory proteins binding to a TATA box) These proteins (the regulatory proteins and the DNA cleavage proteins) are instrumental in gene ... binding to the DNA The choice of a minimally restructuring protein allows one to isolate and examine the issues of interest (here, the intrinsic dynamics of the protein, water dynamics and DNA ... the use of these class of agents is underway (see [35] for further details) Zinc finger proteins, or DNA- binding proteins in general, are the other class of DNA- binding agents This class of agents...

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Báo cáo khoa học: Oxidative stress induces a reversible ﬂux of cysteine from tissues to blood in vivo in the rat pptx

Ngày tải lên : 16/03/2014, 02:20

... LMM-SH and protein sulfhydryls [19,20], without producing free radicals or other ROS, as indicated by the lack of increase in lipid peroxidation observed in in vitro and in vivo experiments [21] In ... in peripheral blood as biomarkers of oxidative stress Clin Chem 52, 1406–1414 22 Di Semplicio P, Giannerini F, Giustarini D, Lusini L & Rossi R (1998) The role of cysteine in the regulation of ... Cysteine ﬂux under oxidative stress 31 32 33 34 35 36 37 38 39 40 41 42 Ames BN, Basu S et al (2005) Biomarkers of Oxidative Stress Study II Are oxidation products of lipids, proteins, and DNA...

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báo cáo hóa học: " Biomarkers of oxidative stress and its association with the urinary reducing capacity in bus maintenance workers" pptx

Ngày tải lên : 20/06/2014, 00:20

... response of the organism to particle-induced oxidative stress The observed increase of reducing species in urine would mirror an increased level in blood originating from a response to oxidative stress ... urinary levels of 8OHdG (in μg/g creatinine) and reduced species (in μW/g creatinine) for all collected samples (b) Correlation between within-shift variation of 8OHdG (% of initial value) and ... Levels of 1-OHP, 8OHdG and reducing species in urine Concentrations of 1-OHP (a), 8OHdG (b) and reducing capacity (c) in urine samples of workers, presented as a function of their smoking status and...

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Báo cáo khoa học: " The effect of methyl sulphonyl methane supplementation on biomarkers of oxidative stress in sport horses following jumping exercise" doc

Ngày tải lên : 12/08/2014, 18:22

... combined with vitamin C (Vit C), on training -induced decrease of reduced Effect of methyl sulphonyl methane (MSM), alone or combined with vitamin C (Vit C), on training -induced decrease of reduced ... combined with vitamin C (Vit C), on training -induced increase of lipid Effect of methyl (LPO) levels Effect of methyl sulphonyl methane (MSM), alone or combined with vitamin C (Vit C), on training ... by increases in lipid peroxidation, glutathione oxidation, and oxidative protein damage [28] In our study exercise induced an increase in cellular oxidative stress (as shown by the increase in...

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Tài liệu Báo cáo Y học: Identiﬁcation and characterization of the Escherichia coli stress protein UP12, a putative in vivo substrate of GroEL pptx

Ngày tải lên : 22/02/2014, 07:20

... other means as an in vivo substrate of GroEL [27] Interestingly, GatY is also included in a list of proteins that aggregate at 42 °C in E coli containing a dnaK deletion mutation (DnaK is a Hsp70 ... members of this family including UspA itself (Fig 2), and one larger protein consisting of two UspA domains in tandem [17] The small members of this family are proteins of 142–144 amino-acids ... that UP12 is a stress protein DISCUSSION In this work, we have identiﬁed two proteins GatY and UP12 as putative in vivo substrates of the chaperonin GroEL In addition to its in vivo interaction...

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Báo cáo khoa học: Calcium, mitochondria and oxidative stress in neuronal pathology Novel aspects of an enduring theme pdf

Ngày tải lên : 07/03/2014, 12:20

... Ca2+ overloading and other factors including oxidative stress [88,89] In neurons the presence of PTP in situ has not gained wide acceptance among investigators and results published in the literature ... buffer the incoming calcium resulting in an abrupt, sustained and irreversible increase in [Ca2+]c, probably coincides with the cleavage of the exchanger (but see [164]) Therefore, inhibition of the, ... transients during reversible brain ischemia Exp Brain Res 104, 462–466 188 Silver IA & Erecinska M (1992) Ion homeostasis in rat brain in vivo: intra- and extracellular [Ca2+] and [H+] in the hippocampus...

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Báo cáo hóa học: " Oxidative stress in NSC-741909-induced apoptosis of cancer cells" potx

Ngày tải lên : 18/06/2014, 16:20

... LOX inhibitors, including aesculetin (a nonselective LOX inhibitor), MK886 (an inhibitor of the 5-LOX-activating protein), zileuton (a 5-LOX inhibitor), baicalein (a 12/15-LOX inhibitor), and ... [11,12], and promote apoptosis In fact, ROS-induced oxidative stress and cell death play important roles in the efficacy of many antineoplastic agents [13,14] To investigate whether oxidative stress ... sustained JNK activation associated with decreased protein levels of MKP1, one of MAP kinase phosphatases that inactivate JNK and p38 MAP kinases [2] Interestingly, NSC-741909 induced an increase...

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báo cáo hóa học: " Neuronal oxidative damage and dendritic degeneration following activation of CD14-dependent innate immune response in vivo" doc

Ngày tải lên : 19/06/2014, 22:20

... Hull M: Mechanisms of prostaglandin E2-induced interleukin-6 release in astrocytes: possible involvement of EP4-like receptors, p38 mitogen-activated protein kinase and protein kinase C J Neurochem ... transcription and increased cytokine secretion occur rapidly and peak within a few hours of LPS exposure As with oxidation of lipoproteins, it is likely that this delay in neuronal oxidative damage ... element of secondary signaling pathways, also completely blocks ICV LPS-induced neuronal oxidative damage Finally, mice lacking prostaglandin E2 receptor subtype (EP2), one of four prostaglandin E2...

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báo cáo hóa học: " Brain inflammation and oxidative stress in a transgenic mouse model of Alzheimer-like brain amyloidosis" pptx

Ngày tải lên : 19/06/2014, 22:20

... indomethacin plus vitamin Effect of1 brain oxidative stress E supplementation on Effect of indomethacin plus vitamin E supplementation on markers of brain oxidative stress Total cerebral cortex homogenates ... animals were divided in two groups (n = 10 each), and randomized to receive placebo, or simultaneously indomethacin (10 mg/liter) in their drinking water, and vitamin E (α-tocopherol) in their diet ... that coincidental suppression of brain oxidative stress further augments the anti-amylodogenic effect of indomethacin Materials and Methods Animals The genotype and phenotype features of the...

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báo cáo hóa học: " The microglial NADPH oxidase complex as a source of oxidative stress in Alzheimer''''s disease" ppt

Ngày tải lên : 19/06/2014, 22:20

... global inhibition of src-family tyrosine kinases or inhibition of phosphatidylinositol-3 kinase (PI3K) attenuates ROS production These findings suggest that these kinases are involved in upstream ... http://www.jneuroinflammation.com/content/3/1/30 kinases Lyn and Fyn as well as the tyrosine kinase Syk [30,32,52] Activation of these signaling cascades are linked to the synthesis and secretion of proinflammatory molecules and cytokines ... the initiation of complex signaling events leading to protein tyrosine phosphorylation and activation of the src-family In both human AD brain tissue [27] and fAβ-treated cultured monocytes and...

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Báo cáo khoa học: "Comparative study of PM2.5 - and PM10 - induced oxidative stress in rat lung epithelial cells" pptx

Ngày tải lên : 07/08/2014, 17:22

... suggesting that PM2.5 induced more oxidative stress than PM10 In the single strand breakage assay using pBR322 DNA, the ratio of changed form II/total plasmid DNA (form Comparative study of PM2.5 ... Briefly, µg of pBR322 DNA (TaKaRa, Japan) was suspended in 50 µl of TE buffer (pH 7.4) containing PM2.5 and PM10 stock, and then incubated at 37oC for day The exposed DNA samples were examined for ... remarkable change of expression level in Nrf-2, proteins related with many transcriptional and anti -oxidative stress enzyme, was observed in the groups of three concentration of PM2.5 and PM10 (5,...

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Báo cáo lâm nghiệp: "Response to an ozone gradient of growth and enzymes implicated in tolerance to oxidative stress in Acer saccharum (Marsh.) seedlings" pot

Ngày tải lên : 08/08/2014, 00:22

... xation of CO2 by PEPC increased in response to O3 in both groups of seedlings (Figs 5E5H) during the growing season The level of activity decreased in the neoformed ush of the episodic seedlings ... processes of injured leaves [23] However, phenol content of truncated seedlings remained unchanged with increasing O3 and even decreased with increasing O3 for episodic seedlings Large progression of ... immersing the tubes for in boiling water The colorimetric determination of NO2 was done by mixing 0.25 mL of incubation medium with 0.25 mL 0.02% N-(1-Naphtyl) ethylenediamine and 0.25 mL of sulfanilamide...

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