what is the trend in reactivity of the group 1 elements with water
Báo cáo khoa học: Structural characterization of the large soluble oligomers of the GTPase effector domain of dynamin potx
Ngày tải lên :
23/03/2014, 11:20
... ‘assembly’
domain and a proline-arginine rich domain at the
extreme carboxy-terminal end. The GTPase domain is
the most highly conserved domain within the members
of the dynamin family. The functional ... dynamin assembles in the form of rings
around the necks of budding vesicles, and then a con-
formational change in the dynamin collar aids the scis-
sion of the vesicle from the parent membrane. The
coiled-coil ... to bind the
middle domain of dynamin [7].
The above possibility of functional dissection of the
dynamin protein into specific domains suggests that a
detailed characterization of the intrinsic...
- 10
- 370
- 0
Báo cáo khoa học: Revisiting the 13C-label distribution of the non-oxidative branch of the pentose phosphate pathway based upon kinetic and genetic evidence doc
Ngày tải lên :
23/03/2014, 15:21
... exchange
2 21 194 13
n
6
12 1 11 9 2
n
7
18 24 36
n
8 net
913 48
n
8 exchange
410 > ;10 0
n
9 net
)3 )567
n
9 exchange
10 15 5 > 10 0
n
10 net
)6 )838
n
10 exchange
12 4 4898 > 10 0
n
11 net
)6 )838
n
11 ... v
12
A3ị
n
9b
ẳ v
9f
ỵ v
10 b
ỵ v
12
A4ị
n
10 f
ẳ v
8b
ỵ v
10 b
ỵ v
13
A5ị
n
10 b
ẳ v
8f
ỵ v
10 f
ỵ v
13
A6ị
n
11 f
ẳ v
14 b
ỵ v
15
A7ị
n
11 b
ẳ v
14 f
ỵ v
15
A8ị
n
12 f
ẳ v
14 f
ỵ v
16
A9ị
n
12 b
ẳ v
14 b
ỵ ... either f6p or p5p,
while in the traditional model they can only originate
from p5p. In a
13
C-labeling experiment with 10 0%
13
C
1
glucose this will result in the synthesis of unlabeled and
13
C
1
-labeled...
- 13
- 443
- 0
A Compilation of the Messages and Papers of the Presidents Section 3 (of 3) of Volume 8: Grover Cleveland, First Term. pptx
Ngày tải lên :
31/03/2014, 11:20
... January, 18 85,
in relation to the management and conduct of the office of district attorney of the United States for the
southern district of Alabama."
The incumbent of this office on the 1st ... promptly to inform the Commission, in writing, of the
removal or discharge from the public service of any examiner in his office, or of the inability or refusal of any
such examiner to act in that ... custody, nor is there magic
in the undefined and sacred solemnity of Department files. If the presence of these papers in the public offices
is a stumbling block in the way of the performance of Senatorial...
- 477
- 487
- 0
Báo cáo khoa học: Cellular refractoriness to the heat-stable enterotoxin peptide is associated with alterations in levels of the differentially glycosylated forms of guanylyl cyclase C pdf
Ngày tải lên :
08/03/2014, 08:20
... concentrations
of
12 5
I-labeled ST
Y72F
for 1 h at 37 °C in binding buffer
(50 m
M
Hepes, pH 7.5, 4 m
M
MgCl
2
, 0 .1% bovine serum
albumin, 10 lgặmL
)1
leupeptin, 10 lgặmL
)1
aprotinin).
Following incubation, ... 10 000 g for 1 h at 4 °C. The pellet obtained
was resuspended in buffer containing 50 m
M
Hepes,
pH 7.5, 5 lgặmL
)1
soybean trypsin inhibitor, 5 lgặmL
)1
leupeptin, 5 lgặmL
)1
aprotinin and 10 0 ... and
nonessential amino acids for 12 h in the absence of ST
peptide, and in the presence of cycloheximide (10 0 lgặmL
)1
)
or swainsonine (10 lgặmL
)1
). Cells were either re-stimulated
with ST peptide,...
- 10
- 427
- 0
Mobil 1 is original equipment in some of the world’s finest vehicles, including potx
Ngày tải lên :
08/03/2014, 19:20
... maximum
350
300
350
200
15 0
10 0
50
0
10 0%
90%
80%
70%
60%
50%
40%
30%
20%
10 %
0%
21, 000
21, 000
42,000
42,000
84,000
84,000
12 6,000
12 6,000
16 8,000
16 8,000
210 ,000
210 ,000
63,000
63,000
10 5,000
10 5,000
14 7,000
14 7,000
18 9,000
18 9,000
2 31, 000
2 31, 000
0
0
...
longer-lasting engines. In 19 74, Mobil 1
synthetic engine oil was born.
The power of Mobil 1 technology gives you the
most advanced engine protection in three ways:
ã Keeps Your Engine Performing ...
than any other brand.
ã Consistently bring new innovations to the
market to meet the latest needs in engine oil.
Discover the
Mobil 1 Difference
What s the Synthetic Difference?
Synthetics
Uniform...
- 14
- 517
- 0
Báo cáo Y học: The role of the second binding loop of the cysteine protease inhibitor, cystatin A (stefin A), in stabilizing complexes with target proteases is exerted predominantly by Leu73 pdf
Ngày tải lên :
17/03/2014, 10:20
... [‡ 7]
1. 1 · 10
)10 b
3.2 · 10
)4c
P74G £ 2.4 · 10
)11
(7) (4.6 ± 0.2) · 10
6
(10 )
[0.9]
£ 1. 1 · 10
)4c
Q76G £ 1. 1 · 10
)11
(8) (6.3 ± 0.2) · 10
6
(9)
[1. 2]
£ 6.9 · 10
)5c
N77G £ 1. 1 · 10
)11
(8) ... · 10
)7
(3)
[0.5] [1. 2] [0.6]
Cathepsin L Wild-type £ 1 · 10
)11 a
5.2 · 10
6a
£ 5 · 10
)5a
[1] [1] [1]
L73G (1. 09 ± 0.08) · 10
)10
(10 ) (2.98 ± 0.04) · 10
6
(10 ) (3.4 ± 0.4) · 10
)4
(3)
[‡ 11 ] ... Search FixingÕ of the side chains within the
Leu73–Asn77 segment in the second binding loop of
cystatin A. After each of these steps, the conformation of
the second binding loop in the complex...
- 10
- 533
- 0
Báo cáo khoa họcRe-engineering the discrimination between the oxidized coenzymes NAD+ and NADP+ in clostridial glutamate dehydrogenase and a thorough reappraisal of the coenzyme specificity of the wild-type enzyme docx
Ngày tải lên :
06/03/2014, 00:20
... 0 .11 4 ± 0. 012 17 9 0.57 ± 0.06 0.26 ± 0. 01 2 .19 81. 7
F238S 7.0 17 .6 ± 0.3 1. 25 ± 0.46 14 .1 0 .12 5 ± 0.04 1. 1 ± 0.7 0 .11 3 12 5
P262S 7.0 23.0 ± 1. 4 1. 04 ± 0 .13 23.0 0.457 ± 0.05 1. 23 ± 0.22 0.3 71 ... Switzerland
Program in Neurosciences & Mental
Health, Hospital for Sick Children, Toronto,
Canada
(Received 5 March 2 011 , revised 21 April
2 011 , accepted 9 May 2 011 )
doi :10 .11 11/ j .17 42-4658.2 011 .0 817 2.x
Clostridial ... splitting off the nicotinamide ring, suggesting
that the covalent bond between the nicotinamide and
the ribose of the coenzyme is particularly labile. The
signal of the parental molecule is also visible...
- 9
- 526
- 0
Báo cáo khoa học: Mass spectrometric characterization of the covalent modification of the nitrogenase Fe-protein in Azoarcus sp. BH72 ppt
Ngày tải lên :
07/03/2014, 02:20
... electrophoresis.
Another striking difference of the MALDI-TOF spec-
tra from the modified Fe-protein in comparison to the
unmodified Fe-protein is the decreased intensity of peak
16 25.4 m ⁄ z and the absence of ... absence of peak 16 16.3 m ⁄ z can be
explained again by the inability of trypsin to cleave
C-terminal to R102 due to the modification at this resi-
due. However, the presence of peak 16 25.5 m ⁄ z in the
spectrum ... PAGE analysis, indicating hydrolysis of the
modification under these conditions even in the
absence of TFA (see Supporting information, Fig. S1
and Doc. S1). The LC liquid phase which contained
formic...
- 10
- 414
- 0
Báo cáo khoa học: Functional studies of crustacean hyperglycemic hormones (CHHs) of the blue crab, Callinectes sapidus – the expression and release of CHH in eyestalk and pericardial organ in response to environmental stress pot
Ngày tải lên :
16/03/2014, 06:20
... decrease in CHH expres-
sion in the ES is in proportion to that of CCAP
expression in the same tissue, as described in Chung
et al. [47], but there is no change in CCAP of TG,
indicating the tissue-specific ... (http://www.dnr.state.md.us/bay/
monitoring /water/ index.html). In particular, it is noted
that in the Bay, low temperatures during winter and
anoxia during summer, in combination with the
changes in salinity, are associated with ... ranging from 1 : 5 for Cal. sapidus,to1: 8in
Car. maenas,to1 :10 inCan. pagurus [29,30], yielding
peak 2 as the major CHH.
The immunohistochemistry studies show that the
intrinsically staining cells...
- 12
- 474
- 0
Báo cáo khoa học: Structure and topology of the transmembrane domain 4 of the divalent metal transporter in membrane-mimetic environments docx
Ngày tải lên :
16/03/2014, 16:20
... found in most of the structures
of the 16 conformers at pH 7.5 in the segment of Leu9–
Val17, except Ile12 and Phe16 in some of the conformers.
Table 1. Structural statistics for DMT1-TM4 in the ... to
explore the molecular biology aspects of DMT1 since the
discovery of this gene, there has so far been no structural
characterization of either this integral protein or a segment
of it. Analysis of the ... 2004 Structure and topology of TM4 of DMT1 (Eur. J. Biochem. 2 71) 19 51
peaks of Ile10, Ile12 and Val17. This suggested that the
peptide was inserted in the interior of micelles. However,
little...
- 14
- 486
- 0
Báo cáo khoa học: The starch-binding capacity of the noncatalytic SBD2 region and the interaction between the N- and C-terminal domains are involved in the modulation of the activity of starch synthase III fromArabidopsis thaliana pdf
Ngày tải lên :
22/03/2014, 21:20
... confirmed the results obtained in the
pull-down assays, showing that there is a physical
interaction between the N- and C-terminal domains of
SSIII in vitro.
Mapping of the CD-binding region in the
N-terminal ... starch-binding domain;
CD, catalytic domain; D123, N-terminal
domain containing the three SBDs; D23,
truncated isoform lacking the D1 domain;
CD–St2 .1, CD–2.2 and CD–2.3, truncated
proteins lacking ... starch-binding domains, particularly
the D( 316 –344) and D(495–535) regions, with the catalytic domains, as well
as the full integrity of the starch-binding capacity of the D2 domain, are
involved in...
- 13
- 457
- 0
Báo cáo khoa học: Change in structure of the N-terminal region of transthyretin produces change in affinity of transthyretin to T4 and T3 pdf
Ngày tải lên :
23/03/2014, 10:21
... revised 14 June
2006, accepted 3 July 2006)
doi :10 .11 11/ j .17 42-4658.2006.05404.x
The relationship between the structure of the N-terminal sequence of trans-
thyretin (TTR) and the binding of ... to
0
2
4
6
8
10
12
14
0 10 20304050607080
0.0
0.5
1. 0
1. 5
2.0
2.5
3.0
0 5 10 15 20 25 30 35 40 45 50
0.0
0 .1
0.2
0.3
0.4
0.5
0 5 10 15 20 25
0.0
0 .1
0.2
0.3
0.4
0246 810 1 214 16
0.0
0.5
1. 0
1. 5
2.0
2.5
3.0
0 ... 7G.
Discussion
The binding of thyroid hormones is one of the main
functions of TTR, which is functionally integrated with
albumin and thyroxine-binding globulin as a network
system to ensure the appropriate...
- 11
- 457
- 0
báo cáo hóa học: " Initial validation of the Argentinean Spanish version of the PedsQL™ 4.0 Generic Core Scales in children and adolescents with chronic diseases: acceptability and comprehensibility in low-income settings" pdf
Ngày tải lên :
18/06/2014, 19:20
... 0.5 18 6 0.80
Emotional 65 ± 21. 31 0 10 0 0.5 5.3 208 0.59
Social 69 .1 ± 21. 67 10 10 0 0 11 .1 203 0.59
School 65.6 ± 21. 3 10 10 0 0 5.2 18 9 0.62
Proxy-Report
Total 73.36 ± 16 .09 14 10 0 0 1. 7 18 3 ... 72.72
(14 . 21)
66.87
(16 .74)
0. 011 71. 64
(17 .13 )
58.54
(15 .8)
71. 31
(17 .07)
65 .16
(15 .45)
65.29
(19 .96)
68.98
(15 .22)
SCT, HIV >
COPD*
Physical 75.42
(15 .93)
67.76
(19 .60)
0.004 74.24
(17 .08)
58 .16
(19 .84)
74.58
(18 .05)
60.98
(19 .44)
67 .17
(1. 32)
72.03
(15 .84)
SCT, ... 4 10 0 0 10 .1 272 0.78
Psychosocial 72. 41 ± 16 .45 18 10 0 0 2.4 18 9 0. 81
Emotional 69 .16 ± 19 .6 5 10 0 0 6.3 285 0.66
Social 77.78 ± 20.73 5 10 0 0 20.2 283 0. 71
School 68.74 ± 24 5 10 0 0 1. 7 19 2...
- 15
- 585
- 0
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