uptake of amino acids and glucose

Báo cáo khoa học: Assimilation of excess ammonium into amino acids and nitrogen translocation in Arabidopsis thaliana – roles of glutamate synthases and carbamoylphosphate synthetase in leaves ppt

Ngày tải lên : 30/03/2014, 01:20

... ve-carbon amino acids) and asparagine and aspartate (four-carbon amino acids) (up to 80% of the total amino acids) (Fig 3) 4068 te te cc mc se Fig In situ hybridization of the transcripts of GLU1, ... biosynthesis and transport of amino acids under photorespiratory and nonphotorespiratory conditions Results Expression of the genes for GOGATs and CPSase In order to understand the physiological role of ... entry of excess ammonium and incoming primary nitrogen into glutamine and glutamate (Fig 8) These amino acids are trafcked under the ne control of amino acid transporters [32,33] Active uptake...

16
384
0

Báo cáo y học: " Effects of carbohydrate, branched-chain amino acids, and arginine in recovery period on the subsequent performance in wrestlers" potx

Ngày tải lên : 11/08/2014, 23:21

... stimulus-secretion coupling of amino acid-induced insulin release: insulinotropic action of br anched-chain amino acids at physiological concentr ations of glucose and glutamine Eur J Clin Invest ... Nishitani S Takehana K: Phar macological activities of br anched-chain amino acids: augmentation of albumin synthesis in liver and improvement of glucose metabolism in skeletal muscle Hepatol Res ... match The effect of branched-chain amino acids and arginine on glucose disposal and performance was also investigated Nine well-trained male wrestlers participated in trials in a random order Each...

46
298
0

Microbial Production of Amino Acids in Japan

Ngày tải lên : 26/10/2013, 02:20

... introduction of the technology, various methods were searched for and developed for microbial production of other amino acids Today a whole array of amino acids are produced by microbial methods and used ... Brevibacterium lactofermentum, and B flavum in the presence of high concentrations of sugar and ammonium ions at neutral pH and under aerobic condition [2] The pathway of biosynthesis of l-lysine and l-threonine ... soybea1ns contain lower amounts of lysine, which is one of Microbial Production of Amino Acids in Japan 77 the essential amino acids for those livestocks The estimated amount of l-lysine produced in...

15
758
5

Báo cáo khóa học: Vertical-scanning mutagenesis of amino acids in a model N-myristoylation motif reveals the major amino-terminal sequence requirements for protein N-myristoylation ppt

Ngày tải lên : 16/03/2014, 16:20

... examine the effect of the combination of amino acids at positions and on protein N-myristoylation, sequential vertical-scanning mutagenesis of the amino acids at positions and in a model N-terminal ... combination of amino acids at positions and might be a critical determinant for protein Nmyristoylation In the present study, to examine the effect of the combination of amino acids at positions and ... addition to the combination of amino acids at positions and 6, it was demonstrated that the combination of amino acids at positions and also affects the susceptibility of the protein to protein...

12
512
0

Báo cáo khoa học: Identiﬁcation of amino acids in antiplasmin involved in its noncovalent ‘lysine-binding-site’-dependent interaction with plasmin pptx

Ngày tải lên : 17/03/2014, 03:20

... half-lives of plasmin in these experiments (also before 50% of the plasmin activity was inhibited) Computer model of the C-terminal 40 amino acids in antiplasmin A computer model of the C-terminal 40 amino ... hydrophobic amino acid distant from the C-terminal portion of antiplasmin seems therefore to be of little importance Expression and puriﬁcation of antiplasmin variants wt-antiplasmin and seven ... added to a mixture of plasmin substrate, 6-aminohexanoic acid and anti-antiplasmin IgG as described in Material and methods After incubation for 90 at room temperature and addition of acetic acid,...

7
292
0

barrett - amino acids and peptides (cambridge, 2004)

Ngày tải lên : 04/06/2014, 16:05

... atom and racemisation of ␣ -amino acids 4.4.7 Reactions of the amide group in acylamino acids and peptides Derivatisation of amino acids for analysis 4.5.1 Preparation of N-acylamino acid esters and ... Chromatographic and related methods for the separation of mixtures of amino acids, mixtures of peptides and mixtures of amino acids and peptides 4.14 Separation of amino- acid and peptide mixtures ... Sources and roles of amino acids and peptides 1.2 Deﬁnitions 1.3 ‘Protein amino acids , alias ‘the coded amino acids 1.4 Nomenclature for ‘the protein amino acids , alias ‘the coded amino acids ...

241
228
0

báo cáo khoa học: "Effective interventions to facilitate the uptake of breast, cervical and colorectal cancer screening: An implementation guideline." pps

Ngày tải lên : 10/08/2014, 11:20

... contributions MB and CD developed the original study concept and protocol MB, CD, and LB were responsible for acquisition and analysis of the data; development of the initial draft manuscript, and manuscript ... for the interpretation of the data; review of the draft versions of the manuscript; provision of feedback for important intellectual revisions; and review and final approval of the version to be ... updates and recommendations provide a valuable source of information to clinicians, policy makers, and researchers internationally who have an interest and mandate to advance quality of cancer...

28
295
0

Báo cáo khoa học: "The concentration of oxygen, lactate and glucose in the central veins, right heart, and pulmonary artery: a study in patients with pulmonary hypertension" ppt

Ngày tải lên : 13/08/2014, 03:20

... ΔSO2 and Δ[Lac] may be of little clinical interest if these gradients are produced exclusively by mixing of SVC and IVC blood streams On the other hand, if ΔSO2 and Δ[Lac] result from mixing of ... heart is the most aerobic of organs, normally deriving its energy from the oxidation of free fatty acids and lactate, and coronary venous blood normally has the lowest SO2 of any venous blood [7] ... exclusively from mixing of SVC and IVC blood streams, we measured concentrations of oxygen and [Lac] in the central veins, the right heart chambers, and the pulmonary artery of hemodynamically stable...

7
415
0

Guanidine catalyzed enantioselective mannich reaction towards the synthesis of amino acids

Ngày tải lên : 10/09/2015, 08:31

... TOWARDS THE SYNTHESIS OF  -AMINO ACIDS PAN YUANHANG (BSc., Zhejiang University) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY DEPARTMENT OF CHEMISTRY NATIONAL UNIVERSITY OF SINGAPORE To ... Mannich reactions of fluorocarbon nucleophiles VIII List of Figures Figure 1.1 Structures of compounds 46 and 47 Figure 1.2 Monitoring of the reaction in DMF-d7 as a function of time at room temperature ... consumption of 82 was incomplete Due to the similar polarity of 82 and 83, it was difficult to separate them Because of these two facts, the yield of 83 was about 30-70% 30 Chapter 2.2.2 Synthesis of...

240
360
0

Báo cáo khoa học: The ATPase activities of sulfonylurea receptor 2A and sulfonylurea receptor 2B are inﬂuenced by the C-terminal 42 amino acids doc

Ngày tải lên : 06/03/2014, 11:20

... equation using a Km of 460 lM, a Vmax of 2.52 nmol PiÆminÆmg)1 and an offset of 0.1 nmol PiÆminÆmg)1 for SUR2A, and a Km of 41 lM, a Vmax of 0.73nmol PiÆminÆmg)1 and an offset of 0.05 nmol PiÆminÆmg)1 ... values of 995 and 878 lM, and Vmax values of 31 and 27 nmol PiÆmin)1Æmg)1 protein, respectively The dashed line is the average of the ATPase activities • of NBD1 and NBD2B Table ATPase activities and ... lm), and NBD2A (529 lm) The different activities of NBD2A and NBD2B could result from an inhibitory effect of the C-terminal 42 amino acids of NBD2B or a stimulatory effect of the equivalent amino...

9
620
0

Báo cáo khoa học: Amino acids at the N- and C-termini of human glutamate carboxypeptidase II are required for enzymatic activity and proper folding pptx

Ngày tải lên : 07/03/2014, 15:20

... region (amino acids 19–43), and four extracellular domains spanning amino acids 44–150 (domain C), 151– 274 (domain D), 275–586 (domain E), and 587–750 (domain F) While the domain spanning amino acids ... the putative zinc ligands, putative substrate-binding residues and other amino acids situated in the vicinity of these residues The results conﬁrmed the importance of the amino acid residues, ... chromatography and chromatofocusing, as described previously [21] Results Expression and secretion of truncated variants of GCPII To analyze the contribution of individual domains of human GCPII...

9
414
0

Báo cáo khoa học: Transport of taurocholate by mutants of negatively charged amino acids, cysteines, and threonines of the rat liver sodium-dependent taurocholate cotransporting polypeptide Ntcp docx

Ngày tải lên : 17/03/2014, 09:20

... uptake because mutations in these amino acids caused lack of transport; the amino acids in question are Asp115, Glu257, and Cys266 All of these are conserved in SBATs and are found also in the human ... importance of negatively charged amino acids for sodium-coupled substrate cotransport or exchange [12,13,16–18] Mutations of all conserved and two nonconserved negatively charged amino acids to ... Asp115Asn and Glu257Gln are also given in a Hill plot Fig The relationship between the amount of injected cRNA and taurocholate uptake into X laevis oocytes via Ntcp and Ntcp mutants Uptake of [3H]taurocholate...

11
367
0

Báo cáo Y học: Amino acids 3–13 and amino acids in and ﬂanking the 23FxxLF27 motif modulate the interaction between the N-terminal and ligand-binding domain of the androgen receptor pdf

Ngày tải lên : 17/03/2014, 10:20

... Results of the yeast protein interaction assay are shown in Fig 8(B) Substitution of amino acids 23, 26 and 27 completely abolished interaction with GalDBD-AR.LBD and alanines at positions 24 and ... function of AR NTD and thus does not allow discrimination between loss of AR.NTD-AR.LBD binding and loss of AR.NTD transactivating function In the yeast assay, loss of transactivation function of AR ... results are presented in Fig In the absence of ligand, none of the AR NTD proteins showed LBD interaction However, in the presence of ligand, both AR.NTDwt and AR.NTDD3– 13 bound to AR LBD with similar...

12
597
0

Báo cáo khoa học: Arg143 and Lys192 of the human mast cell chymase mediate the preference for acidic amino acids in position P2¢ of substrates pdf

Ngày tải lên : 22/03/2014, 21:21

... aromatic amino acids (Phe, Tyr, and Trp); negatively charged amino acids (Asp and Glu); positively charged amino acids (Lys and Arg); small aliphatic amino acids (Gly and Ala); larger aliphatic amino ... amino acids (Val, Leu, Ile, and Pro); and hydrophilic amino acids (Ser, Thr, His, Asn, Gln, Cys, and Met) The nomenclature of Schechter and Berger [5] was adopted to designate the amino acids ... Arg143 and Lys192 are essential in conferring a strong preference for acidic amino acids at position P2¢ of the substrates Table P2¢ speciﬁcity and amino acids found in positions 40, 143 and 192 of...

13
424
0

Báo cáo khoa học: Chromophore attachment in phycocyanin Functional amino acids of phycocyanobilin – a-phycocyanin lyase and evidence for chromophore binding doc

Ngày tải lên : 23/03/2014, 10:21

... Comparisons of relative enzymatic activities of CpcE ⁄ F and their mutants, of covalent binding of PCB to CpcE and CpcF, and of their capacity for transferring PCB All test were done under standard ... CpcE(L276D) Fig (A, B) Comparison of conserved N- and C-terminal domains in CpcE and PecE from different organisms, and (C) of N-terminal amino acids in CpcF and PecF CLUSTAL W (1.8) multiple ... amounts of CPC [20,25,26] Homologous lyases of the ‘E ⁄ F-type’ are involved in the attachment of PEB and PCB to C84 Chromophore binding by a-phycocyanin lyase of the a-subunits of CPE and PEC,...

13
436
0

Characterization of new peptides and physiological amino acids present in cerebrospinal fluid of chronic pain patients

Ngày tải lên : 12/09/2015, 09:55

... Figures xiv List of Tables xvi List of Abbreviations Chapter One Introduction An Overview of Pain, its physiology, classification and molecular mechanisms of pain, role of amino acids and known peptides ... concentration of pain related amino acids 79 3.3 CSF concentration of other amino acids not related to pain 81 Chapter 4.1 Comparison of concentration of pain-related amino acids in CSF Acute labor ... Three An analysis of amino acid neurotransmitters and nitric oxide in acute labor pain Applying our new method for amino acid analysis in CSF to analysis of physiological amino acids and other pain...

218
264
0

Effects of novel purine analogs and the role of aromatic amino acids on MRP4 functions

Ngày tải lên : 05/10/2015, 13:53

... localized on chromosome 10q24 and consists of 32 exons encoding a polypeptide of 1545 amino acids The membrane topology for MRP2 is like that of MRP1 and consists of 17 TM helices, which form ... chromosome 17q21.3 and contains a single ORF of 1527 amino acids, with a predicted molecular mass of 169.4 kDa The membrane topology predicted for MRP3 is like that of MRP1 and MRP2 and comprises ... recognition and transport activity have been identified A series of studies have shown that mutation of individual aromatic, polar, and charged amino acids can affect the recognition and transport of...

139
544
0

Biodegradable Polymers Composed of Naturally Occurring α-Amino Acids

Ngày tải lên : 25/10/2013, 08:20

... Gomurashvili, Z., Katsarava, R., and Kricheldorf, H.R (2000) Amino acid based bioanalogous polymers Synthesis and study of new poly(ester amide)s composed of hydrophobic α -amino acids and dianhydrohexitoles ... C.C., and Won, C.Y (1999) Amino acid-based bioanalogous polymers Synthesis, and study of regular poly(ester amides) based on bis(α -amino acid) α,ω-alkylene diesters, and aliphatic dicarboxylic acids ... S.N., Yarmush, M.L., and Kohn, J (1993) Copolymers of lysine and polyethylene glycol: a new family of 129 130 Biodegradable Polymers Composed of Naturally Occurring α -Amino Acids 51 52 53 54 55...

26
348
0

Tài liệu Báo cáo khoa học: Modulation of cyclin D1 and early growth response factor-1 gene expression in interleukin-1b-treated rat smooth muscle cells by n-6 and n-3 polyunsaturated fatty acids pdf

Ngày tải lên : 19/02/2014, 16:20

... control of SMC proliferation 4466 S Bousserouel et al (Eur J Biochem 271) Modulation of cyclin D1 synthesis and hyperphosphorylation of Rb by n-3 and n-6 PUFAs Induction of cyclin D1 is one of the ... Modulation of smooth muscle cell (SMC) proliferation and retinoblastoma protein (Rb) phosphorylation after supplementation with n-3 and n-6 polyunsaturated fatty acids (PUFAs) (A,B) Eﬀects of n-3 and ... Rb and Egr-1, in order to determine how the incorporation of EPA and DHA modulate SMC proliferation This article describes the differing effects of n-3 and n-6 PUFAs on the proliferation of SMC...

12
499
0

Tài liệu Báo cáo khoa học: The mechanism of a-proton isotope exchange in amino acids catalysed by tyrosine phenol-lyase doc

Ngày tải lên : 19/02/2014, 16:20

... side chain of any bound amino acid The lability of the a-proton observed for a large number of amino acids [5] under the action of TPL gives evidence for the orthogonal orientation of the a-proton ... with respect to the cofactor plane [17], and shows that the pattern of binding is the same for a variety of amino acids It has been established [5] that for a number of amino acid inhibitors ... Center (TW00106) to R.S.P and T.V.D References Konnikova, A.S., Dobbert, N.N & Braunstein, A.E (1947) Labilization of a-hydrogen of amino acids under the action of aminoferase Biokhimia 12, 556–568...

7
532
0