the vapor pressure of a pure liquid depends on which of the following
Báo cáo khoa học: Mutual effects of proton and sodium chloride on oxygenation of liganded human hemoglobin Oxygen affinities of the a and b subunits potx
Ngày tải lên :
16/03/2014, 14:20
... O
2
affinity, K , can be derived for both
the a and b subunits from the averaged parameters of
HbA oxygenation (Table 1, Average). The association
and dissociation rate constants for the b subunits
are found ... after
photodissociation, d, is calculated as the ratio of the
quantum yield of BR, c, to that of the primary quan-
tum yield of photodissociation, c
0
.
Having the individual parameters of bimolecular
oxygenation ... NaCl.
Fig. 4. The parameters of O
2
binding to the a and b subunits within liganded hemoglobin as a function of NaCl concentration. Bars 1, 2 and
3 are the relative changes in the association (kÂ),...
- 11
- 577
- 0
Reliability analysis of a power system based on the multi state system theory
Ngày tải lên :
03/01/2014, 19:38
... 22.8
ss
RG=≥
}
Equation (1) indicates that when the capacity of the system
is above 22.8 Ah, the system is reliable, though the capacity of
a battery is lower than 5700 mAh. Suppose the capacity of the
first ... reliability theory defines the power
system and the batteries are b
inary, but they are all multi-state
actually. The performance of the batteries can degrade, which
results in performance degradation ...
G
is the
capacity of the battery.
2
150
()
2
~ 6000,150GN
To analyze the reliability of the system by the traditional
system reliability theory, we must gain the reliability of the
battery...
- 4
- 407
- 0
Tài liệu Báo cáo khoa học: Structural effects of a dimer interface mutation on catalytic activity of triosephosphate isomerase The role of conserved residues and complementary mutations pptx
Ngày tải lên :
18/02/2014, 11:20
... Restriction site
W11F WT W11F CA
CCATGGCTAGAAAATATTTTGTCGCAGCAAACTTCAAATGTAA NcoI
W168F WT W168F GAACCTTTATTCGCTATT
GGTACCGGTAAA KpnI
WT* W11F W11F ⁄ W168F GAACCTTTATTCGCTATT
GGTACCGGTAAA KpnI
Y74W* ... calibration standards provided by the manu-
facturer. Data processing was performed using the deconvo-
lution module of the data analysis software to detect the
multiple charge states and obtain ... maintaining the geometry of the
active site. The availability of crystal structures of
TIMs from 21 sources and the large database of TIM
sequences from various sources facilitate an analysis of
mutational...
- 15
- 635
- 0
Tài liệu Báo cáo khoa học: Application of a fluorescent cobalamin analogue for analysis of the binding kinetics A study employing recombinant human transcobalamin and intrinsic factor pdf
Ngày tải lên :
19/02/2014, 05:20
... rough
comparison of the dissociation velocities indicated at
least a 10-fold faster liberation of the fluorescent ana-
logue when compared with Cbl. The CBC dissociation
spanned at least 90% of the total ... °C was incubated over time. Free ligands
were adsorbed on charcoal, and the absorbance spectra
were recorded. Concentration of appearing IF–CNCbl was
calculated by comparison with the standards ... respects. An alternative
explanation seems to be more probable. Thus, distor-
ted shape of the analogue causes partial corruption of
its bonds with IF. As a consequence, the ligand and
the protein...
- 12
- 603
- 0
Tài liệu Báo cáo khoa học: Production and characterization of a secreted, C-terminally processed tyrosinase from the filamentous fungus Trichoderma reesei ppt
Ngày tải lên :
19/02/2014, 06:20
... TCA TCA
TCA TCA TCA GGG CAC GAC ACA CAT CCC C; and
reverse primer, GAT CGG TAC CTC ATT ACA GAG
GAG GGA TAT GGG GAA C. The PCR reaction was
done as described above. The amplified PCR product was
inserted ... was amplified from genomic T. reesei DNA
with the following primers: forward, GGG GAC AAG
TTT GTA CAA AAA AGC AGG CTA TCA TGC TGT TGT
CAG GTC CCT CTC G; and reverse, GGG GAC CAC
TTT GTA CAA GAA ... ⁄ TOF fragment ion analysis as well as
by Edman degradation of the corresponding peptide
after purification by reversed-phase chromatography.
During the search for and confirmation of the N-ter-
minus...
- 14
- 650
- 0
Tài liệu Báo cáo khoa học: Purification and characterization of a sialic acid specific lectin from the hemolymph of the freshwater crab Paratelphusa jacquemontii pdf
Ngày tải lên :
21/02/2014, 00:20
... purification.
Erythrocyte preparation
Blood for HA assay was prepared as described by
Ravindranath et al. [12].
Hemagglutination assay
Hemagglutination assays were performed in microtiter
plates (Falcon) as recommended ... of animal glycoconjugate. They act
as an important component of the ligands recognized by the
lectins. Recognition can be affected by specific structural
variations and modifications of sialic acids ... sialic
acid may change with transformation or other alteration in
the environment of the cell [63]. The normal human tissues
contain NeuAc, while malignant tumour cells contain
O-acetyl sialic acid...
- 8
- 616
- 0
Tài liệu Báo cáo Y học: Targeting of malate synthase 1 to the peroxisomes of Saccharomyces cerevisiae cells depends on growth on oleic acid medium pptx
Ngày tải lên :
22/02/2014, 04:20
... extra-
peroxisomal Aco1p.
As mentioned previously, malate synthase catalyses the
formation of malate from glyoxylate and acetyl-CoA, the
source of the latter being either peroxisomal when breaking
down ... (Fig. 4A) .
To examine whether a cytosolic malate synthase was as
efđcient as a peroxisomal one for m aintaining a functional
glyoxylate cycle on oleic acid, liquid growth assays were
conducted. The ... cytosolic
supernatant. Equal fractions of each of the protein prepa-
rations (10% of total vol) were i mmobilized on replicate
membranes to which were applied antibodies against Mls1p
or yeast peroxisomal...
- 8
- 444
- 0
Tài liệu Báo cáo Y học: Interallelic recombination is probably responsible for the occurrence of a new as1-casein variant found in the goat species potx
Ngày tải lên :
22/02/2014, 04:20
... amplication
betweenC7UandC8L.Primersinitalicswereusedinthegenotyping
of allele M.
C1U
5Â GAG AGG AAC TGA ACA GAA CAT TG 3Â
C1L 5Â CAA CTG CGT ATT AGT GAA GAA TG 3Â
C2U 5Â AAT CAA ATT TTA TTA TAA GAC C 3Â
C2L
5Â AAT AGC TAA TTA GAG ACC AT 3Â
C3U 5Â GGT GTC AAA ... 5Â ATA CAC ACA AAC TCA CAA GG 3Â
MWU 5Â CAA CAT ATT TTA AAT AAA ATT GAC AAT 3Â
C9LM* 5Â ATA AAA ATG GTA TAC CTC ACT TGT*C 3Â
C9UM1 5Â TAA CAA TGA TTC TCT TTC TTT TAG 3Â
C9LM1 5Â AAT CTT TAT TTT ... TGG CAC AAC ATT GTA CAT TCT TGG G 3Â
C9U 5Â GTA TGG AAG TGT GGA ATA GTT T 3Â
C9L 5Â GGA CAC CAC AGA TAT CCA ATA G 3Â
C1011U 5Â CAT AAA ACT AAC AAT ACA TGT 3Â
C1011L 5Â TAG CAG ATA TTG AAA AGG AG...
- 11
- 568
- 0
Tài liệu Báo cáo Y học: Exploring the role of a glycine cluster in cold adaptation of an alkaline phosphatase pdf
Ngày tải lên :
22/02/2014, 04:20
... value almost 2.5-fold higher than
the native cold adapted enzyme (Table 1). The mutant
G26 1A/ Y26 9A exhibits an E
a
almost the same as in the case
ofthenativeenzyme(Table1).
Thermal inactivation ... and
overexpression of the gene encoding alkaline phosphatase
from the Antarctic strain TAB5 [16]. Based on the crystal
structure (at 2.4 A
˚
)ofanEscherichia coli alkaline phospha-
tase variant ... with the aromatic ring of Tyr269, and these
unfavorable interactions could lead to a decrease of local
flexibility and an increased E
a
value.
The validity of the above interpretation was further
reinforced...
- 6
- 488
- 0
Báo cáo khoa học: Identification and characterization of a nuclear receptor subfamily I member in the Platyhelminth Schistosoma mansoni (SmNR1) pot
Ngày tải lên :
07/03/2014, 11:20
... DR2:
5Â-CCGTAAGGTCACAAGGTCACTCG-3Â,DR3:5Â-CCG
TAAGGTCACAGAGGTCACTCG-3Â, DR4: 5Â-CCGTAA
GGTCACAGGAGGTCACTCG-3Â, DR5: 5Â-CCGTAAGG
TCACCAGGAGGTCACTCG-3Â. PAL0: 5Â-CGCAAGGT
CATGACCTCG-3Â. One strand of each oligonucleotide was
annealed after ... [21]. A SmNR1 specific probe of 497 bp was
produced by PCR amplification with TOPO 2.1-SmNR1 as
a template (forward primer: 5Â-ATTTCAGAAGTTGAAC
AAACACAC-3Â, reverse primer: 5Â-AAGATGGTATT
GAAGATGATGGTTGA-3Â), ... gene in mammalian cells suggests that
SmNR1 ⁄ SmRXR1 can interact with mammalian coac-
tivators of transcription, and S. mansoni coactivators
of transcription may have a similar mechanism to
SmNR1...
- 16
- 542
- 0
Báo cáo khoa học: O-MACS, a novel member of the medium-chain acyl-CoA synthetase family, specifically expressed in the olfactory epithelium in a zone-specific manner doc
Ngày tải lên :
08/03/2014, 02:20
... to
produce acyl-CoA on the outer membrane of mitochondria.
Acyl-CoA is then transported into the matrix for
b-oxidation of acyl-group. Among the MACS family
proteins with the acyl-CoA synthetase activity, ... (5Â-GAATTCatgaaggttctcctccactg-3Â)
and rO-MACS-XhoI-D (5Â-CTCGAGtgcccgtccccactcctggt-3Â)
for o-macs; EcoRI-mMACS1-U (5Â-GAATTCatgcagtggc
tgaagagttt-3Â) and mMACS1-XbaI-D (5Â-TCTAGAtagct
gaccaaactccttg-3Â)forMACS1, ... Yoshihara, Y., Kawasaki, M., Tamada, A. , Fujita, H., Hayashi,
H., Kagamiyama, H. & Mori, K. (1997) OCAM: a new member of
the neural cell adhesion molecule family related to zone-to-zone
projection...
- 10
- 393
- 0
Báo cáo khoa học: Characterization of a high-affinity binding site for the pea albumin 1b entomotoxin in the weevil Sitophilus ppt
Ngày tải lên :
08/03/2014, 02:21
... than the susceptible
weevil strains. Surprisingly, the microsomal fraction of an
extract of the resistant insect D. melanogaster also displayed
a strong binding capacity.
The determination of the ... increasing
concentrations of
125
I-labelled PA1b (0.35–3.2 n
M
).
Table 2. Effect of proteinase K on the
125
I-labelled PA1b binding
activity on the microsomal fraction of S. oryzae WAA42 extract.
Treatment
a
Binding ... post-translational cleavage of the
albumin proprotein PA1, also releasing a second peptide
(PA 1a) . PA1b consists of 37 amino acids, with six cysteines
involved in three disulfide bonds which confer the...
- 7
- 604
- 0
Báo cáo khoa học: On the mechanism of a-amylase Acarbose and cyclodextrin inhibition of barley amylase isozymes pdf
Ngày tải lên :
08/03/2014, 08:20
... corresponding
dissociation constants. In this equation, it was easier for a
calculated constant to compare its value relative to zero
rather than to obtain a large value. When the association
constant value was ... discrepancy was explained by the higher affinity
of acarbose for PPA as indicated by the lower dissociation
constant of the acarbose-PPA complex (1.7 l
M
)[24] .The
dissociation constant of the acarbose-AMY ... l
M
decreasing to 37.3 l
M
by addition of acar-
bose. A
0
is the AMY1 absorbance without acarbose, A is the
absorbance measured at the above acarbose concentrations.
(B) Reciprocal plot of the difference...
- 9
- 437
- 0
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- tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam
- điều tra đối với đối tượng giảng viên và đối tượng quản lí
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- phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ
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