the essential role of protein dephosphorylation in the modulation of bdnf signaling events

Báo cáo khoa học: Essential role of the C-terminus in Melanocarpus albomyces laccase for enzyme production, catalytic properties and structure pdf

Ngày tải lên : 16/03/2014, 00:20

... show the critical role of the last amino acids in the C-terminus of MaL Deletion of the four last amino acids strongly reduced the expression of the protein in T reesei and S cerevisiae Furthermore, ... one amino acid in the C-terminus of the laccase, giving the Tr(L559G) and Sc(L559A) mutants, reduced the turnover of the mutant proteins For the substrate ABTS, the Michaelis constant of the Sc(L559A) ... preventing binding of oxygen We analyzed the azide inhibition and determined the inhibition constants for sodium azide, in order to see the effect of the mutation (L559A) on the binding properties in...

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Báo cáo y học: "Familial Polycythemia Caused by a Novel Mutation in the Beta Globin Gene: Essential Role of P50 in Evaluation of Familial Polycythemi" potx

Ngày tải lên : 08/08/2014, 16:23

... this interface All these substitutions can affect the cooperative nature of oxygen binding with heme, and in turn, can change the affinity of Hb for oxygen The majority of mutations affecting ... BPG) level in the red cell and temperature, and globin structure [13] Affinity of Hb with oxygen is expressed as the P50, which is the partial pressure of oxygen in blood at which 50% of the Hb is ... reflects an increased affinity of hemoglobin for oxygen and vice versa Elevations and reductions in 2, 3- BPG level in the Int J Med Sci 2007, erythrocyte will also lead to corresponding changes in P50...

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Báo cáo khoa học: "Small interference RNA profiling reveals the essential role of human membrane trafficking genes in mediating the infectious entry of dengue virus" pdf

Ngày tải lên : 12/08/2014, 04:21

... reductions in the levels of the respective proteins when compared to the levels in the mock-transfected cells (Figure 3) At the concentration of 25 nM of the transfected siRNA for the respective proteins, ... clathrin molecules (Additional File 3) Together, these data suggest the involvement of clathrin in the endocytosis of the DENV particles To affirm the role of clathrin-mediated endocytosis in DENV ... regulates other endocytic proteins [32] The complex interactions of these cellular proteins are believed to have important roles in controlling the endocytosis kinetics and possibly the origins and...

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Báo cáo y học: " Essential role of platelet activation via protease activated receptor 4 in tissue factor-initiated inflammation" ppt

Ngày tải lên : 09/08/2014, 10:23

... activation in the vascular lining [10] Fibrin, the final product of the coagulation cascade, can also be proinflammatory Fibrin induces endothelial expression of adhesion molecules [11], and the fibrin ... Effect of sTF injected into the footpad: μg sTF (in 10 μl phosphate-buffered saline) was injected into the intraplantar region of the right hindfootpad The contralateral footpad was injected with the ... by administration of the thrombin inhibitor hirudin and an inhibitor of FVIIa Depletion of fibrinogen by ancrod also attenuated inflammation in this model These findings confirm that fibrin formation...

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báo cáo khoa học: " Efficacy of Mesenchymal Stem Cells in Suppression of Hepatocarcinorigenesis in Rats: Possible Role of Wnt Signaling" doc

Ngày tải lên : 10/08/2014, 10:21

... evaluating the tumor suppressive effects of MSCs on the in vivo progression of HCC, and to investigate the possible role of Wnt signaling in tumor tissues by assessing the gene expression profile of ... mimicking the one of resident cells in the host tissue Insults trigger the release of chemokines from the endothelium inducing adhesion and migration of circulation BMDC into the liver parenchyma The ... development of HCC seems to be the abnormal regulation of the transcription factor b-catenin, a key component of the Wnt signaling pathway [58] In the normal state, the binding of members of a family of...

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role of wnt signaling and glycogen synthase kinase-3 beta in adipocyte biology

Ngày tải lên : 14/11/2014, 09:42

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Tài liệu Báo cáo khoa học: Mitochondrial targeting of intact CYP2B1 and CYP2E1 and N-terminal truncated CYP1A1 proteins in Saccharomyces cerevisiae ) role of protein kinase A in the mitochondrial targeting of CYP2E1 pdf

Ngày tải lên : 18/02/2014, 16:20

... Protein targeting to the microsomes requires the cotranslational insertion of the newly synthesized protein into the microsomal membrane, where the N-terminal hydrophobic signal sequence of the ... consistent with the model suggesting a transmembrane topology of CYPs with a single N-terminal membrane anchor and most of the remaining protein exposed to the cytosolic side [15,17,30,31] The intramitochondrial ... localized inside of the inner membrane Moreover, the imported + ⁄ 1A1 and + 33 ⁄ 1A1 proteins were extractable with alkaline Na2CO3 as shown in Fig 2C, suggesting that both proteins are localized in the...

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Tài liệu Báo cáo khoa học: The role of the ESSS protein in the assembly of a functional and stable mammalian mitochondrial complex I (NADH-ubiquinone oxidoreductase) pptx

Ngày tải lên : 19/02/2014, 16:20

... domain extending into the intermembrane space In the third mutant (V79-G35) one might expect a protein to be inserted into the inner membrane, but it is missing a major portion of the domain localized ... served as another identiﬁcation of the complex at the position of the histochemical stain in the left panel Antisera against the SDHC subunit of complex II and against the Rieske protein of complex ... N-terminal domain is involved in protein protein interactions with other hydrophilic domains of neighboring integral membrane subunits, and interspecies interactions are incompatible From the comparative...

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Tài liệu Báo cáo khoa học: The role of the SEA (sea urchin sperm protein, enterokinase and agrin) module in cleavage of membrane-tethered mucins pdf

Ngày tải lên : 19/02/2014, 18:20

... fragments were then cloned into the SpeI ⁄ XhoI sites of the polylinker in MUC1FDTR The remaining portions of the polylinker introduced leucine and valine residues N-terminal to the MUC3 and MUC12 ... domain in the processing of membrane-tethered mucins The mechanism by which the heavily glycosylated portion of these molecules is released at epithelial surfaces within the airway, the intestine ... containing the transmembrane region and the cytoplasmic tail These two parts of the protein remain associated by noncovalent interaction, probably involving sequences within the SEA domain The...

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Báo cáo khoa học: Studies on the role of the receptor protein motifs possibly involved in electrostatic interactions on the dopamine D1 and D2 receptor oligomerization pdf

Ngày tải lên : 07/03/2014, 03:20

... trafﬁcking properties The binding experiments also pointed to a decrease in the density of the D2R variants in the cellular membrane; the number of D2 receptor binding sites decreased with the number ... cytoskeletal protein 4.1 N, a member of the 4.1 family, facilitates the transport of D2R to the cell surface by interacting with the N-terminal portion of the ic3 loop of D2R via its C-terminal domain ... Measuring receptor dimer- ization by monitoring changes in the ﬂuorescence lifetime of probes linked to the receptors of interest seems the best approach in this kind of the study Although the...

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Báo cáo khoa học: The essential tyrosine-containing loop conformation and the role of the C-terminal multi-helix region in eukaryotic phenylalanine ammonia-lyases docx

Ngày tải lên : 07/03/2014, 12:20

... is involved in the biosynthesis of the photoactive yellow protein chromophore of these bacteria The two recently discovered aminomutases, the phenylalanine 2,3-aminomutase (PAM) involved in taxol ... date The rarity of PAL in bacteria may be explained by the infrequency of phenylpropanoids in these species The bacterial PALs seem to be involved in biosynthesis of the antibiotics enterocin by ... of the Tyr110-loop in PAL in more detail Tyr-loop in phenylalanine ammonia-lyases Results and discussion Modeling the active conformation of the essential Tyr110-loop of parsley PAL Because of...

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Báo cáo khoa học: Death inducer obliterator protein 1 in the context of DNA regulation Sequence analyses of distant homologues point to a novel functional role docx

Ngày tải lên : 07/03/2014, 21:20

... regulation These homologues include members of the CpG binding protein (CGBP) family of proteins that bind DNA at unmethylated CpG islands [7], which are always located to active chromatin Other proteins ... maintain transcription The PHF3 protein was recovered in initial analyses and also contains a TFS2M domain showing the same architecture as the DIDO1 long isoform, although lacking the dPHD The ... alignment of death inducer obliterator protein (DIDO), PHF3 and yeast proteins Additional proteins were included Naming conventions are as in Fig S1 The solid purple box indicates the TFS2M domain Pink-boxed...

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Báo cáo khoa học: The role of Tyr71 in Streptomyces trypsin on the recognition mechanism of structural protein substrates ppt

Ngày tải lên : 16/03/2014, 00:20

... placed in the same direction, and a cassette containing the Gmr and E coli rpsL+ genes was inserted between them rpsL+ encodes the ribosomal protein S12 [23], the target of Sm The transformation of ... are shown The recombination sites of the chimeras are boxed The names of the chimeras are indicated in bold capital letters The catalytic residues are indicated in red Identiﬁcation of amino acid ... equivalent in ninhydrin colour to 1.0 lmol of leucine in h at pH 7.4 at 37 °C in the presence of CaCl2 Data are expressed as the mean ± SD of three independent experiments (C) The ratio of hydrolytic...

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Báo cáo khoa học: Role of the structural domains in the functional properties of pancreatic lipase-related protein 2 pot

Ngày tải lên : 16/03/2014, 06:20

... N2Cc In conclusion, the accessibility of the active site was better in the protein bearing the N2 domain than in the protein bearing the Nc domain Thus, the accessibility of the active site in the ... sequences of each protein The chimeric protein composed of the N-terminal domain of hoPL and of the C-terminal domain of hoPLRP2 was named NcC2 Conversely, the other chimeric protein, bearing the N-terminal ... both the N-terminal and C-terminal domains of hoPL were involved in the inactivation of the protein at the water–triglyceride interface The inactivation of N2Cc in the absence of bile salts was...

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Báo cáo khoa học: Isothermal unfolding studies on the apo and holo forms of Plasmodium falciparum acyl carrier protein Role of the 4¢-phosphopantetheine group in the stability of the holo form of Plasmodium falciparum acyl carrier protein docx

Ngày tải lên : 16/03/2014, 10:20

... crucial role in stabilizing the proteinprotein interface [31] All of the interactions reported in Fig in the range between and 6.0 A are identical in the major and minor fractions of the holoprotein ... between the two proteins (as indicated by the values of DGwater, Cm and Tg) hence arise from the changes in the surface of the protein because of the interactions of the cofactor with the protein ... The chaotrope-induced unfolding was almost fully reversible in both forms of the protein Removal of the perturbation makes the protein regain its native form The unfolding reactions of both forms...

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Báo cáo khoa học: Role of CCP2 of the C4b-binding protein b-chain in protein S binding evaluated by mutagenesis and monoclonal antibodies docx

Ngày tải lên : 17/03/2014, 09:20

... on the b-chain in immobilized C4BP In uence of protein S binding of monoclonal antibodies To investigate whether protein S and MoAb 15/ MoAb 44 had overlapping binding sites on the b chain, the ... immobilized recombinant b-chain was tested The binding of both MoAb 15 and MoAb 44 to the b-chain was Ó FEBS 2003 The role of CCP2 of the C4BP b-chain in binding of protein S (Eur J Biochem 270) ... for binding to the antibody (Fig 6) (d) In the fourth assay, the effect of MoAb 44 on the binding of protein S to immobilized b-chain was investigated In this assay protein S was found to bind...

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A Single-Molecule Perspective on the Role of Solvent Hydrogen Bonds in Protein Folding and Chemical Reactions pptx

Ngày tải lên : 22/03/2014, 18:20

... rapidly, causing the unfolding of one of the I27 modules in the chain Unfolding then extends the overall length of the protein, relaxing the pulling force to a low value As the slack in the length ... bridging the A’ and G b-strands of the I27 protein during the main unfolding barrier.[39] To further validate this view and gain insight into the role of solvent hydrogen bonds in protein unfolding, ... trajectories of individual proteins Indeed, using these techniques, it becomes possible to explore the role of the solvent environment in protein collapse[42] and the dynamics of protein folding.[47] Therefore,...

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Báo cáo khoa học: Characterization of the role of a trimeric protein phosphatase complex in recovery from cisplatin-induced versus noncrosslinking DNA damage potx

Ngày tải lên : 30/03/2014, 04:20

... not in the immunopellets from the control cells that did not express the tagged proteins (Fig 3C, top panel) HA3–Pph3p interacted with increasing amounts of cH2AX generated in the presence of increasing ... role in the cisplatin response [9] Cisplatin interferes with DNA function by causing intrastrand and interstrand crosslinking of nucleotide bases and, in replicating cells, DNA damage usually induces ... and the presence of ionizing radiation [12], and in the present study, in the absence and the presence of cisplatin In addition, we showed that Pph3p directly or indirectly binds to cH2AX, indicating...

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Báo cáo khoa học: Dissecting the role of protein–protein and protein–nucleic acid interactions in MS2 bacteriophage stability potx

Ngày tải lên : 30/03/2014, 11:20

... changes in protein conformation in the presence of denaturing agents The tryptophan residues buried in the hydrophobic interior of the protein emit ﬂuorescence when excited at 280 nm When the protein ... produced when coat protein is overexpressed in E coli from a plasmid [11] To examine the stability of the coat protein dimer in the absence of the intersubunit interactions found in the capsid, we ... questions in this work We sought to determine the role of protein RNA and protein protein interactions in virus stability, measuring the effects of urea, GdnHCl and high pressure on the structure...

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Báo cáo khoa học: The role of ADAM10 and ADAM17 in the ectodomain shedding of angiotensin converting enzyme and the amyloid precursor protein ppt

Ngày tải lên : 30/03/2014, 14:20

... both ADAM10 and TACE are involved in the shedding of APP, neither ADAM is involved in the shedding of ACE Furthermore we show that APMA can distinguish between the shedding of ACE and APP Materials ... dose-dependent increase in the shedding of ACE (Fig 3B,C), with a 12-fold increase in the amount of soluble ACE in the CHO cell medium observed with 500 lM APMA The effect of APMA on the shedding of ACE ... cells in the absence or presence of APMA Following this second incubation, the level of sAPPa in the medium was examined (Fig 4A,B) There was no signiﬁcant difference in the level of sAPPa in the...

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