... Purpose — Describe a high- throughput method for the analysisof uncertain models, e.g in biological research Methods — Generalized modeling for conceptual analysisof large classes of models Results ... Generalized Models for high- throughputanalysisof uncertain nonlinear systems Thilo Gross1,2 , Stefan Siegmund∗2 Max-Planck Technical Institute for the Physics of Complex Systems, N¨thnitzer ... efficient exploration of large parameter spaces The ongoing revolution in systems biology is revealing the structure of important systems For understanding the functioning and failure of these systems,...
... Purpose — Describe a high- throughput method for the analysisof uncertain models, e.g in biological research Methods — Generalized modeling for conceptual analysisof large classes of models Results ... Generalized Models for high- throughputanalysisof uncertain nonlinear systems Thilo Gross1,2 , Stefan Siegmund∗2 Max-Planck Technical Institute for the Physics of Complex Systems, N¨thnitzer ... efficient exploration of large parameter spaces The ongoing revolution in systems biology is revealing the structure of important systems For understanding the functioning and failure of these systems,...
... Purpose — Describe a high- throughput method for the analysisof uncertain models, e.g in biological research Methods — Generalized modeling for conceptual analysisof large classes of models Results ... Generalized Models for high- throughputanalysisof uncertain nonlinear systems Thilo Gross1,2 , Stefan Siegmund∗2 Max-Planck Technical Institute for the Physics of Complex Systems, N¨thnitzer ... efficient exploration of large parameter spaces The ongoing revolution in systems biology is revealing the structure of important systems For understanding the functioning and failure of these systems,...
... go to Prof Andrew Tay, Prof David Keezer and Prof Rao Tummala for their help and support Lastly, I would like to thank my family and friends I am thankful to my parents, Michael Tan and See Siew ... bandwidth of GHz and an insertion loss of 12 dB at GHz, the target high- speed I/Os of the specifications Therefore, it is not suited for the required wafer level packaged (WLP) vii device test and ... selection of materials were important as well It often involved a lot of trade-offs between mechanical and electrical performance However, meeting the requirements of being capable of probing...
... Ala and Glu, Glu and Asp, and Glu and Gln is efficient in wheat germ extract but can effectively be suppressed by inhibitors of transaminases and glutamine synthase [20] Among the multitude of ... produced in high yields in vivo are also suitable for efficient production by cell-free synthesis In order to compensate for the increased effort and expense required for the production and selective ... scrambling and easier sample handling compared to in vivo protein production render cell-free protein synthesis particularly attractive for high- throughput production of proteins and selective...
... platform has been designed to maintain common standards forhigh quality IS annotation used in ISfinder at both protein and nucleotide levels It is a web-based service that includes an ensemble of ... Module (a): if the file is pre-annotated, the protocol performs a BLASTP (filter off and e-value 1e-5) analysis followed by BLASTX (filter off and e-value 1e-5) to identify any ORFs that may have ... annotation is performed prior to BLASTP and BLASTX Identified ORFs are included in a candidate ORF list The replicon is then subject to BLASTN (filter off, word size and e-value 1e-5) analysis, which...
... MIP-1β The chemokine MIP-1α occurs in two highly related isoforms, designated LD78α and LD78β, and although they only differ in three amino acids, the LD78β isoform is much more potent as a CCR5 agonist ... availibity of stable and reliable in vitro models is a prerequisite for the successful setup of an accurate screening program for chemokine receptor antagonists Here, we Page of 13 (page number not for ... concentrations (IC50) of SCH-C for inhibition of LD78β-induced calcium flux were ng/ml in the single and ng/ml in the double-transfected cells For AMD3100, the IC50 values for inhibition of SDF-1-induced...
... DEVELOPMENT AND APPLICATION OF ADVANCED PROTEOMIC TECHNIQUES FOR HIGHTHROUGHPUT IDENTIFICATION OF PROTEINS HU YI (B.Sc.) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY DEPARTMENT OF BIOLOGICAL ... Tandem Affinity Purification TOF Time of Flight TOF/TOF Time -of- Flight/Time -of- Flight UV Ultraviolet Y2H Yeast Two-Hybrid xvi Chapter Introduction The complete sequence of the human genome (Lander ... study of the complete set of genetic information of an organism (Mckusick, 1997), which encompasses mapping, sequencing andanalysisof the whole genome of an organism The significance of genomics...
... Regional Office for the Americas (AMRO), Dr Vijay Chandra, WHO Regional Office for South-East Asia (SEARO), Dr Custodia Mandlhate, WHO Regional Office for Africa (AFRO), Dr Claudio Miranda (AMRO), ... specialist services is high in comparison with that of other forms of service delivery The reasons for this include the high level of investment required to set up dedicated units and the high staff costs ... Regional Office for the Eastern Mediterranean, Dr Wolfgang Rutz, WHO Regional Office for Europe (EURO), Dr Erica Wheeler (WHO/HQ), Dr Derek Yach (WHO/HQ), and staff of the WHO Evidence and Information...
... for the discovery of novel miRNA genes A computational approach called miRTRAP (miRNA Tests for Read Analysisand Prediction) was developed for the systematic prediction of miRNAs from high- throughput ... used as candidate loci to be tested as a possible miR Our approach for the identification of microRNAs using high- throughput sequencing reads is to compute a set of quantities for each candidate ... positions of reads The distance between a miR and moR on the same arm of the hairpin, the offset of the 5' positions of products that overlap at least nucleotides on the same arm of the hairpin, and...
... ‘mate pair’ and ‘end sequence profiling’ strategies; Figure 1) [6] The genome is broken into DNA fragments of selected size, for example 500 base pairs (bp) [5], and a short sequence, for example ... small tandem duplications, inversions and deletions were entirely within genes In many cases this affected the exon structure at the transcript level and novel isoforms were observed Some of these ... mutations, that is, random mutational noise, but some must be selected, ‘driver’ events and, as the number and variety of known mutations increases, estimates for the number of ‘driving’ mutations...
... those of binding site representationand discovery [Stormo 2000], both of which we address in this thesis The first problem is to develop a representationfor a group of known binding sites of a ... position of a group of aligned sequences and the relative frequency of each amino or nucleic acid at that position (see Figure 1.1) 1.2.1 Our contributions: a comparative analysisofrepresentationand ... These values for Centroid are 2% and 9%, andfor PSSM are 10.5% and 8% 2.3.4 Statistical significance of method comparisons To compare methods and assess whether the differences in performance (partially...
... function OBJ2 and OBJ3 are unitless, and the unit 1/ud of emission factors EMSsp, EMSpw and EMSwc, and 1/uw of risk index RKsp, RKpw and RKwc are applied in order to eliminate the units of Eqs and 3, ... purchasing costs, capacity and risk index of each supplier s, and the fixed costs, unit operational costs and capacity of the candidate locations for production plant p and warehouse w are presented ... delivery time, and defects of raw materials acquired from suppliers Amin and Zhang [39] propose a bi-objective model for closed-loop SCND with inexact input information on demands and return, and the...
... amplifiers handle signals in and out of the antenna ◗ The RF and power section handles power management and recharging, and also deals with the hundreds of FM channels Thirty years ago, all of that ... devices, and SMS (Short Message Service) for cellular phones Disruptive innovations offer a different, original and often untested solution to a larger category of needs [25] They offer better performance ... new markets for high- tech firms [26]: ◗ IBM, Sun, Hewlett-Packard, and Microsoft push for autonomic computing: Enabling a computer system to diagnose and optimize its own performance and allocate...
... to organize a review and evaluation of current practice and to develop a set of principles and guidelines for good practice in the digital representationand management of cultural heritage The ... works of art, prints, out-size material and artifacts To achieve the highest quality scans of transparent media (e.g 35mm slides and negatives, 6x4 and large format transparencies and microfilm) ... objects; the use of speech recognition for the indexing and retrieval of digitized speech archives; and research into video analysisand indexing systems that can parse and analyze hours of video, identify...
... The specific methods and software required for the analysisof these and related datasets are discussed in Chaps and 1.4.3 Understanding More About RNA by Sequencing DNA High- throughput sequencing ... different steps of a highthroughput sequencing project The “newbies” eager to learn the basics ofhighthroughput sequencing technologies and data analysis will find what they yearn for specially ... al Fig 2.1 High- throughput sequencing workflow There are three main steps in high- throughput sequencing: preparation, immobilization, and sequencing Preparation of the sample for highthroughput...