... indicate the position
and orientation ofthe primers. Bold font
represents the sequence of mature miR9*
and italic fonts the sequence of mature
miR9. (C) RT-PCR analysis of pri-miR9s dur-
ing the ... vector. The pro-
moter sizes ofthe construct are indicated
by numbers in parentheses. )44 means
located 44 bp upstream ofthe rst base pair
at the 5Â-end of pre-miR-9-1 dened as +1.
FLuc is the ... miR9-1PN5_Fluc, were amplified
using the primer pairs listed in Table 1, and then sequenced
to determine the orientation ofthe fragments in the repor-
ter vector. These constructs were then transfected into...
... G
4
and the sidechain of Asn122. In the second model
(Fig. 1B) the structure ofthe hexanucelotide is, on the
whole, unchanged and the majority ofthe interactions that
were seen in the first model are ... attributed solely to the change in
charge and size ofthe sidechain of this single residue, the
crystal structure of RTA N122A was solved. The RMSD
from the C
a
atoms ofthe wild-type RTA crystal ... and, as such, these mutants
could not be purified. To assess the effect of substitutions
made at each ofthe arginyl residues on catalytic activity of
RTA, the ability of each ofthe purified RTA...
... [11,12]. The elevation of
these sand ridges is about 2-7m, their width
varies from 100 to 3000m. They distributed
parallel to the shore in the form of bows with a
distance from each other about ... in coastal zone of Mekong delta
consist of 18 facies which are expressed on the
map ofthe Holocene deposital environments
(fig.7). However, in this paper ones important
of them were only ... facies at the end.
A group of submarine Middle-Early
Holocene marine facies composed of two
facies: a shallow marine sands and gravelly
sands are spread from 25 m water depth
shallow sea. They...
... this segment ofthe business accounted for just over 73 percent of
the fund market at end June 2011, with the remaining 27 percent composed of non-UCITS.
Table 7. Net Assets ofthe European ... billion during the first half of 2011, slightly behind
the EUR 55 billion recorded in the first half of 2010. Long-term UCITS attracted EUR 87 billion during the
first half ofthe year, down ... including funds of funds, except for France, Germany and Italy for which the funds of funds data are
included in the other fund categories; (3) net sales of non-UCITS are included in "other"...
... this segment ofthe business accounted for almost 71 percent ofthe
fund market at end June 2012, with the remaining 29 percent composed of non-UCITS.
Table 7. Net Assets ofthe European ... including funds of funds, except for France, Germany and Italy for which the funds of funds data are
included in the other fund categories; (3) net sales of non-UCITS are included in "other" ... assets of balanced funds reduced by 2.1 percent. Funds of funds registered an increase
in net assets of 1.7 percent during the quarter.
Over the first half of 2012, total net assets of UCITS...
... between the projection ofthe tilt ofthe z axis on the xÂ,y plane (dened direction of tilt of z and the x axis), and j % a + c denes the
location ofthe rhombic (x, y) axes. The orientation ofthe ... at position E7 as
the source ofthe H-bond to ligand on the basis of a
partial sequence, which had indicated a Tyr on the distal
E-helix. The similarity in the
1
HNMRspectraofthe
three globin ... ligand.
Comparison ofthe strengths of dipolar interactions in
solution with the alternate crystal structures of methemo-
globin establish that the solution structure of wild-type Hb
more closely resembles the...
... affected the level ofthe mRNA
for the other ADAM, confirming the specificity of these
ASOs [19]. The ASOs reduced the level of their respective
proteins in cell lysates but had little effect on the other
protein ... IC
50
of 80 n
M
[14], we considered whether the
APMA-induced shedding of ACE was mediated by TACE.
The effect ofthe TACE ASO, along with the ADAM10
ASO, on the APMA-induced shedding of ACE was
therefore ... in the presence or absence
of APMA. In the majority of cases there was little difference
between the effect of inhibitors on the constitutive shedding
of ACE and the APMA-induced shedding of...
... and appropriate. The power of the
photographs was the result of plain scenic attrac-
tiveness ofthe landscape seen through the eyes of
the general Icelandic public, as well as of refer-
ences ... about
the politics of landscape. The question I would like
to pose next is: What kind of aesthetic philosophy
might best further the cause of an invigorated poli-
tics of landscape?
Reasserting the ... had the unfor-
tunate side-effect of stifling serious discussion
among geographers about the aesthetic politics of
landscape.
There is no denying the importance ofthe visual.
What is more, there...
... [15].
The second electron rereduces Cu
A
thereby forming the
initial two-electron reduced oxidase. In the absence of
oxygen or the presence of CO, there is probably a two-
electron reduction ofthe ... other factors. None-
theless, catalytic activity is still one ofthe few characteristics
that reflect the actual role ofthe oxidase in the tissues.
What is that role? The oxidase catalyzes the ... for the samples to freeze;
during the freezing process, the sample undergoes osmotic
stress. The vapor pressure ofthe ice surrounding the
oxidase is lower than the vapor pressure of water in the
protein....
... Whether these two bodies of law conflict or co-exist is beyond
the scope of this chapter, but the question is important to an understanding of the
law relating to theprotectionofthemarineenvironment ... themarineenvironment and the land
environment. This is scarcely surprising, given that the greatest impact on the
marineenvironment comes not fr
om the use ofthe sea but fr
om the use ofthe ... any definition of a marine region. It does,
however introduce the idea of integrating theprotectionofthemarine and coastal
environment, requiring states to manage themarineenvironment and...
... Overview ofthe biological effects of lost and
discarded plastic debris in themarine environment. Marine
Pollution Bulletin 18, 319–326.
Laist, D.W., 1997. Impacts ofmarine debris: entanglement of marine
life ... 1992).
Since the use of plastics continues to increase, so does
the amount of plastics polluting themarine environ-
ment. Robards et al. (1995) examined the gut content of
thousands of birds in ... waters
and the pore waters ofthe sediments, and the resulting
hypoxia or anoxia in the benthos can interfere with the
normal ecosystem functioning, and alter the make-up
of life on the sea floor...
... reefs off the continental United States.
244,245
The Keys
comprise 88 percent of coral reefs in Florida, excluding the reefs ofthe Middle Grounds
situated 100 miles off the coast in the Gulf of ... Indiana, covers the bulk of Marion County in the central part ofthe state, pop-
ulation 860,454. The city is situated in the upper part ofthe White River watershed—a
system of rivers draining ... enforcement of environmental laws. The
organization was founded by Eric Schaeffer, former director ofthe U.S. Environmental
Protection Agency’s Office of Regulatory Enforcement, with support from the...