issues beyond the debate on the moral status of the human embryo stem cell biology and regenerative medicine

Screen of nuclear receptors for the enhanced and alternative generation of induced pluripotent stem cells

Ngày tải lên : 09/09/2015, 18:56

... ablation of Klf5 prevented the successful derivation of mouse ESCs from the ICM, and on the other hand, the overexpression of Klf5 in mouse ESCs suppressed differentiation in the absence of LIF60 ... regulation… 1.2 Embryonic stem cell, the common precursor cell …………………… ……….3 1.3 Important transcription factors governing the ESC fate: Oct4, Sox2 and Nanog 1.4 The first derivations of mouse and human ... assume distinct cellular functions and different morphologies 1.2 Embryonic stem cell, the common precursor cell Intriguingly, regardless of the dissimilarities of the myriad somatic cell types present...

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báo cáo khoa học: "Epigenomics of human embryonic stem cells and induced pluripotent stem cells: insights into pluripotency and implications for disease" potx

Ngày tải lên : 11/08/2014, 12:21

... embryonic stem cell; H3K4me1, methylation of lysine of histone H3; H3K4me3, trimethylation of lysine of histone H3; H3K27ac, acetylation of lysine 27 of histone H3; H3K27me3, trimethylation of ... CTCF Figure The combination of stem cell models and epigenomics in studies of the role of non-coding mutations in human disease Epigenomic analyses of cells derived through in vitro stem cell differentiation ... pluripotent stem cell; H2AZ, histone variant H2AZ; H3ac, acetylation of histone H3; H4ac, acetylation of histone H4; H3K4me1/2/3, (mono-, di- and tri) methylation of lysine of histone H3; H3K27ac,...

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Báo cáo y học: " Induced pluripotent stem cells free of exogenous reprogramming factors" pot

Ngày tải lên : 14/08/2014, 21:20

... integration included the repeated transient transfection of plasmidbased vectors into mouse embryonic fibroblasts [5], and the use of adenoviruses in mouse liver cells [6] However, in both cases, the ... When these iPSCs were induced to differentiate under neuronal differentiation protocols, dopaminergic neurons were derived regardless of the age of the donor, thus highlighting the feasibility of ... integration of transgenes into the genome, and the exogenous DNA can be removed by gradual loss of the episomes during extended culture without drug selection, and without the need for further genetic...

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Generation of porcine induced pluripotent stem cells and their differentiation into cardiac lineages

Ngày tải lên : 30/09/2015, 10:13

... until the edges of the cell colony starts to curl of the surface of the dish The dispase solution is then aspirated and fresh hESCmedia added The cell colonies are then lifted of the surface of the ... expressed in the pluripotent lineage of the mouse embryo, and it is also expressed in the multipotent cells of the ectoderm layer in the extraembryonic regions and also in the precursor cells of the developing ... Culture of Mouse Embryonic Fibroblast (MEF) cells as feeder cells for hESCs 37 4.2 Culture of Human Embryonic Stem Cells (hESCs) 38 4.2.1 Culture of Human Embryonic Stem Cells on MEF...

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Atlas of Human Pluripotent Stem Cells doc

Ngày tải lên : 22/03/2014, 09:20

... amenable for long-term culture, expansion and manipulation of human pluripotent stem cells The culmination of two events has enabled the production of human embryonic stem cells: the birth of the ﬁrst ... of our team’s experience, skill and knowledge in the derivation, culture and expansion of human embryonic stem cells, and more recently, of human induced pluripotent stem cells The exploration ... translational research in the ﬁeld of embryonic and induced pluripotent stem cells It commences with practical aspects of the derivation and growth of human embryonic stem cells from inner cell mass...

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Báo cáo toán học: "Adult neurogenesis, neuroinflammation and therapeutic potential of adult neural stem cells" ppsx

Ngày tải lên : 08/08/2014, 17:20

... implications for cellular therapy On the one hand, it may limit the therapeutic potential of adult NSCs in vivo and ex vivo On the other hand, it may interact with the neurogenic niches to promote the ... Newborn neuronal cells may represent a regenerative attempt and contribute to the plasticity of the nervous system [73] (fig 1) There are however debates and controversies over the modulation of adult ... unraveling the molecular mechanisms governing the interaction between neural progenitor and stem cells and the immune system, and it implications for cellular therapy Conflict of interest The author...

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báo cáo khoa học: " Capturing Alzheimer’s disease genomes with induced pluripotent stem cells: prospects and challenges" pps

Ngày tải lên : 11/08/2014, 12:21

... purify NPCs and neurons from differentiated hESCs and iPSCs For the purification of neurons, the methods of both Pruszak et al [46] and Yuan et al [47] rely on neuronal expression of the heat-stable ... further characterization of the additional cell types present in cultures (both neuronal and nonneuronal) or the development of methods to isolate cell types of interest iPSCs and hESCs generally ... characterize the differentiation process and the resultant cultures For neuronal cultures, informative measurements include the proportion of neurons in culture, the subtypes of neurons present, and the...

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Báo cáo y học: "Hypertrophy is induced during the in vitro chondrogenic differentiation of human mesenchymal stem cells by bone morphogenetic protein-2 and bone morphogenetic protein-4 gene transfer" pps

Ngày tải lên : 09/08/2014, 14:22

... by the proximity of the cells to the joint space and the bone Page 10 of 15 (page number not for citation purposes) respectively, alignment of the chondrocytes along the arcades of Benninghoff ... and CS4, one of the monomers of the polysaccharide portion of proteoglycan, showed significantly enhanced production of these cartilage matrix proteins at days 10 and 21 of culture in the aggregates ... BMP-4 and approximated the size of lacunae noted in the calcifying zone of the human growth plate In contrast, the lacunae that formed in the presence of BMP-4 were closer in size to those of the...

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báo cáo khoa học: " Transcriptomic analysis of pluripotent stem cells: insights into health and disease" potx

Ngày tải lên : 11/08/2014, 12:21

... group proteins Non-coding RNA network Active Figure The embryonic stem cell transcriptional regulatory circuit The embryonic stem cell (ESC) transcription factors Oct4, Sox2 and Nanog form an ... dysregulation of gene expression [29] ESC, embryonic stem cell; hiPSC, human induced pluripotent stem cell; iPSC, induced pluripotent stem cell; miRNA, microRNA complex to control neural crest formation ... cell; hESC, human embryonic stem cell; hiPSC, human induced pluripotent stem cell; H3K4me3, histone H3 lysine trimethylation; iPSC, induced pluripotent stem cell, lincRNA, long intergenic non-coding...

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Báo cáo y học: "Discrepancy between the in vitro and in vivo effects of murine mesenchymal stem cells on T-cell proliferation and collagen-induced arthritis" doc

Ngày tải lên : 12/08/2014, 11:23

... isolation and characterization of MSCs; MSC stimulation, quantitative PCR, and Bio-Plex; CIA induction and evaluation; humoral and cellular responses; analysis of T -cell proliferation; design of the ... study; and manuscript preparation TM and HK contributed to MSC stimulation, quantitative PCR, and Bio-Plex; CIA induction and evaluation; humoral and cellular responses; and analysis of T -cell ... proliferation LG contributed to CIA induction and evaluation and to analysis of T -cell proliferation PM contributed to the design of the study and to manuscript preparation All authors contributed...

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Báo cáo y học: "Low temperature tolerance of human embryonic stem cells"

Ngày tải lên : 31/10/2012, 16:57

... Becker EW The roles of ATP in the dynamics of the actin filaments of the cytoskeleton Biol Chem 2006;387(4):401-6 Lopez LA, Bertini F The in vivo effect of low body temperatures on the degree of polymerization ... emphasized the use of temperature-heated stage and pre-warmed and pre-equilibrated culture media Nevertheless, the results of this study would prove otherwise hESC of the H9 line isolated by Thomson and ... Jones JM Embryonic stem cell lines derived from human blastocysts Science 1998;282(5391):1145-7 Reubinoff BE, Pera MF, Fong CY, Trounson A, Bongso A Embryonic stem cell lines from human blastocysts:...

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Báo cáo khoa học: Recruitment of transcription complexes to the b-globin locus control region and transcription of hypersensitive site 3 prior to erythroid differentiation of murine embryonic stem cells docx

Ngày tải lên : 07/03/2014, 12:20

... and was only detected at the core regions [14,15] Localization of RNA Pol II to the LCR was independent of active transcription elongation; the addition of the elongation inhibitor DRB did not ... Discussion B Fig Transcription of LCR hypersensitive site in undifferentiated murine embryonic stem cells and in human CD 133+ bone marrow cells (A) Transcription of LCR regions and the bmaj-globin gene ... leukemia inhibitory factor (LIF) These cells express the Rex-1 and b-actin genes but not the embryonic and adult globin genes (Fig 2) Upon differentiation the embryonic- and adult-speciﬁc b-globin...

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báo cáo hóa học:" MicroRNA and gene expression patterns in the differentiation of human embryonic stem cells" doc

Ngày tải lên : 18/06/2014, 15:20

... expression profiles of miRNAs and transcripts using three hES cell lines, embryonic bodies (EB) produced from one of the cell lines and five types of terminally differentiated adult cells The integration ... Journal of Translational Medicine 2009, 7:20 similar inferred function in modification of chromatin structure Methods Cell culture and embryoid body differentiation Human embryonic stem cell lines ... transcriptional network for pluripotency of embryonic stem cells Cell 2008, 132(6):1049-1061 Heo JS, Lee MY, Han HJ: Sonic hedgehog stimulates mouse embryonic stem cell proliferation by cooperation of...

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Báo cáo sinh học: " Phosphoproteomic analysis of apoptotic hematopoietic stem cells from hemoglobin E/b-thalassemia" pot

Ngày tải lên : 18/06/2014, 19:20

... characterization of phosphorylation is critical to the elucidation of signal transduction pathways, the understanding of the mechanism of disease progression, and the development of therapeutic applications ... populations of these cells for in vitro study of the cellular processes in thalassemic stem cells While previous attempts have focused on the cell culture system, in this study the phosphoproteome of the ... using fresh human hematopoietic stem cells for studying cell signaling in the apoptotic pathway because of the limited amount of sample from patients and normal donors On the other hand, in vitro...

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báo cáo hóa học:" Elevated adipogenesis of marrow mesenchymal stem cells during early steroid-associated osteonecrosis development" pptx

Ngày tải lên : 20/06/2014, 00:20

... further divided into sub-single-lesion group (SON group: with one ON lesion) and sub-multiple-lesion group (MON group: with more than one ON lesion) Statistics The differences between ON+ and ON- ... period Of the 25 rabbits, 15 were found ON+ (60%) and 10 were ON- (40%) Of the 15 ON+ rabbits, rabbits had only one ON lesion and were classified to SON group, rabbits had more than one lesion and ... prevention modalities There are many postulations about the pathogeneses of steroid-associated ON One of them is the theory of lipid deposition, i.e the deposited marrow fat would compress on blood...

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o cáo hóa học:" Phosphoproteomic analysis of apoptotic hematopoietic stem cells from hemoglobin E/b-thalassemia" pptx

Ngày tải lên : 20/06/2014, 04:20

... characterization of phosphorylation is critical to the elucidation of signal transduction pathways, the understanding of the mechanism of disease progression, and the development of therapeutic applications ... populations of these cells for in vitro study of the cellular processes in thalassemic stem cells While previous attempts have focused on the cell culture system, in this study the phosphoproteome of the ... using fresh human hematopoietic stem cells for studying cell signaling in the apoptotic pathway because of the limited amount of sample from patients and normal donors On the other hand, in vitro...

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Báo cáo y học: "Biology of adult mesenchymal stem cells: regulation of niche, self-renewal and differentiation" potx

Ngày tải lên : 09/08/2014, 10:20

... their pleiotropic effects Examples of canonical Wnt functions include the promotion of long-term culture expansion of stem cells, increased in vivo reconstitution of hematopoietic lineages, and ... maintains the stem state of MSCs [14] and other stem cells [15] LIF also activates and represses osteoblast and osteoclast activities [20] The bipotency of LIF suggests that the cellular environment and ... all of these tissues, or MSCs function autonomously, in a manner that is independent of their environment? Since Schofield first introduced the concept of a stem cell ‘niche’ in 1978 [65], the...

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Báo cáo y học: "Chordin knockdown enhances the osteogenic differentiation of human mesenchymal stem cells" pdf

Ngày tải lên : 09/08/2014, 10:23

... all donors tested Another factor to consider is whether the age of the donors had an influence on the results of this study Four donors were over 70 years old and one donor was aged 19 The relative ... Cheng SL: Regulation of bone matrix protein expression and induction of differentiation of human osteoblasts and human bone marrow stromal cells by bone morphogenetic protein-2 J Cell Biochem 1997, ... Expression of BMP-2 and chordinstem cells during osteogenic differentiation of human mesenchymal stem cells (a) Temporal progression of bone morphogenetic protein (BMP)-2 and chordin expression during...

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Fibronectin and laminin promote differentiation of human mesenchymal stem cells into insulin producing cells through activating Akt and ERK pptx

Ngày tải lên : 10/08/2014, 05:21

... LAM on the phosphorylation status of Akt and ERK for stage III cells There was a baseline of Akt and ERK phosphorylation without adding ECM FN and LAM increased phosphorylation of Akt and ERK, and ... serine/threonine kinase promotes cell cycle progression, cell survival, and tumour cell invasion [23] The b -cell proliferation and differentiation are regulated by various growth factors and hormones, ... contributions HYL and CCT assisted with conception and design, collection and assembly of data, data analysis and interpretation, and manuscript writing, LLC assisted with collection and assembly of...

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Báo cáo y học: "Hedgehog overexpression leads to the formation of prostate cancer stem cells with metastatic property irrespective of androgen receptor expression in the mouse model" pps

Ngày tải lên : 10/08/2014, 05:21

... metastatic loci The data evidently demonstrated both cancer cell and stem cell characteristics of the transformed P63+ basal /stem cells, supporting that they could be the origin of PCSCs under the influence ... based on several lines of observations Firstly, the entire process of prostate tumorigenesis was reconstituted in vivo and the effects of Hh overexpression on the normal prostate stem cells was ... http://www.jbiomedsci.com/content/18/1/6 Page of 11 Figure Differentiation status and AR expression profile of PCSCs under the influence of Hedgehog overexpression The boxed areas in the pictures are further magnified...

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