isolation and culture of rodent bone marrow derived multipotent mesenchymal stromal cells

Báo cáo y học: "Sequestration and homing of bone marrow-derived lineage negative progenitor cells in the lung during pneumococcal pneumonia" ppsx

Ngày tải lên : 12/08/2014, 15:21

... Number of QD-labeled donor cells in BM = Fraction of donor cells in BM × total number of BMCs The number of BMCs harvested from femurs and tibias was considered to represent 18.1% of total murine marrow ... tissue injury, and showed engraftment of both type II [20,21] and type I [19] pneumocytes from BMderived cells demonstrating the ability of these cells to participate in structural repair of the lung ... measured The numbers of donor cells in circulation and BM were calculated as follows: Number of QD-labeled donor cells in circulation = Fraction of donor cells in MNCs × fraction of MNCs × white blood...

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Proteomic analysis of the response of murine bone marrow derived macrophages to IFN y stimulation and infection with staphylococcus aureus

Ngày tải lên : 12/05/2016, 21:57

... disposal of damaged or senescent red cells, wound healing and tissue repair Macrophages infiltrate tumours and lysis of tumour cells by monocytes and macrophages are thought to be a mechanism of host ... experimental system - Bone marrow derived macrophages in serum-free culture Murine bone marrow derived macrophages (BMMs) are a good model for studying macrophage biological processes and functions including ... stimulation, and S aureus infection were performed by staff of the Institute of Medical Microbiology, University of Greifswald, Germany Preparation and cultivation of mouse stem cells and their...

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báo cáo hóa học:" Isolation and culture of fibroblasts from endoscopic duodenal biopsies of celiac patients" pdf

Ngày tải lên : 18/06/2014, 15:20

... http://www.translational-medicine.com/content/7/1/40 Q-Banding Cells in log phase were cultured with 50 μL of colchicine for hours and mitotic cells were gently blown with a pipette Cells were centrifuged at 235 × g for 10 minutes and the ... positive for FSP and negative for cytokeratin 20 in all the cultured and examined cells (Figure 3) Discussion Q-banding analysis of FBs from CD and non-CD subjects demonstrated a normal and stable ... immune system and the presence of autoantibodies [1,2] Different cell types (enterocytes, lymphocytes B and T, macrophages, dendritic and mesenchymal cells) participate in the development of the CD...

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Báo cáo y học: " Peripheral infusion of rat bone marrow derived endothelial progenitor cells leads to homing in acute lung injury" pps

Ngày tải lên : 12/08/2014, 15:20

... Characteristics of ex vivo-generated bone marrow- derived rat EPC Cells were harvested from the non-adherent fraction of rat bone marrow mononuclear cells after 48 h of culture on fibronectin-coated culture ... Figure Proliferative characteristics of bone marrow- derived EPC Proliferative characteristics of bone marrow- derived EPC EPC and rPAEC were cultured in the presence of 20% FCS in EBM-2 without further ... in terms of an increase of bone marrow- derived progenitor cells in the circulation h after exposure by about a factor of four, an accumulation of those cells within inflammatory sites and then...

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báo cáo hóa học:" Biocompatibility of Poly-ε-caprolactone-hydroxyapatite composite on mouse bone marrow-derived osteoblasts and endothelial cells" doc

Ngày tải lên : 20/06/2014, 01:20

... numbers of adult EPCs may provide sufficient seeding cells for tissue engineering applications Bone marrow stromal cells (MSCs) are multipotent stem cells originating from the bone marrow stroma, and ... osteogenic and endothelial cells Methods Cell Culture and Induction Bone marrow cells were obtained from male BALB/c mice (6–8 weeks of age) Low-density bone marrow mononuclear cells (MNCs) were isolated ... 4D) Figure Morphology of osteoblasts and endothelial cells Morphology of osteoblasts and endothelial cells During the 2nd passage, the mice bone marrow- derived mononuclear cells differentiated...

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báo cáo hóa học: " Comparison of immature and mature bone marrow-derived dendritic cells by atomic force microscopy" potx

Ngày tải lên : 21/06/2014, 02:20

... streptomycin, and adjusted to × 109/L Induction and separation of bone marrow- derived dendritic cells The above cells were seeded into a 6-well plate to the end volume of mL per well, and 10.0 μg/L of rmGM-CSF ... comparison and a P value of less than 0.05 was considered statistically significant Results and discussion Morphologic and functional characteristics of BMDCs The bone marrow cells were cultured and ... μg/L of rmIL4 (PeproTech) was added to the corresponding wells in the plate and cultured at 37.0°C in an incubator containing 5% CO2 to induce differentiation of bone marrow cells into bone marrow- derived...

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Augmentation of neovascularization in murine hindlimb ischemia by combined therapy with simvastatin and bone marrow-derived mesenchymal stem cells transplantation pptx

Ngày tải lên : 10/08/2014, 05:21

... Page of 10 Figure Characterization of bone marrow- derived MSCs Flow cytometric analyses of bone marrow- derived MSCs Cells were uniformly negative for CD34, CD45, and positive for CD44, and CD29 ... with NIH image software Effect of simvastatin on the cell viability of bone marrowderived MSCs in vitro To examine whether simvastatin has anti-apoptotic effect on bone marrow- derived MSCs under ... Effect of simvastatin on the cell viability of bone marrow -derived MSCs in vitro In vitro, serum starvation induced bone marrow- derived MSCs apoptosis, as indicated by flow cytometry and MTT...

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Báo cáo y học: "Phenotypic and functional abnormalities of bone marrow-derived dendritic cells in systemic lupus erythematosus" pot

Ngày tải lên : 12/08/2014, 12:20

... viability of the feeder cells in long-term culture assays, thus reducing the interference of continued growth of these cells on the proliferation of the co-cultured responder cells [19] Cell cultures ... controls BMDCs, bone marrow- derived dendritic cells; BMCs, bone marrow cells; FLDCs, dendritic cells induced by FL; mDCs, myeloid dendritic cells; FL, FMS-like tyrosine kinase ligand; GM-CSF, granulocyte ... Gershwin ME: Expansion of bone marrow IFN-alpha-producing dendritic cells in New Zealand Black (NZB) mice: high level expression of TLR9 and secretion of IFNalpha in NZB bone marrow J Immunol 2004,...

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Báo cáo y học: "Effects of bone marrow-derived cells on monocrotaline- and hypoxia-induced pulmonary hypertension in mice" pps

Ngày tải lên : 12/08/2014, 15:20

... recipient) number means marrow- derived cells recipient), Effect of the number of bone marrow- derived cells on the increases in right ventricular systolic pressure (RVSP) and right ventricle to ... by a single bone marrow- derived stem cell Cell 2001, 105:369-377 Kotton DN, Ma BY, Cardoso WV, Sanderson EA, Summer RS, Williams MC, Fine A: Bone marrow- derived cells as progenitors of lung alveolar ... Representative panel of a control mouse lung (A and B) constitutively expressing GFP and wild-type mouse lung (C) injected with eGFP bone marrow- derived cells (BMDCs) Panels D, E, F and G are representative...

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Báo cáo y học: "ntravenous transplantation of allogeneic bone marrow mesenchymal stem cells and its directional migration to the necrotic femoral head"

Ngày tải lên : 25/10/2012, 11:18

... therapy of hip osteonecrosis with autologous bone marrow grafting Indian J Orthop 2009 Jan;43(1):40-45 Sun Y, Feng Y, Zhang C The effect of bone marrow mononuclear cells on vascularization and bone ... experience and a review Cancer, 2003;97(10):2453-2461 Lazarus HM, Haynesworth SE, Gerson SL, et al Ex vivo expansion and subsequent infusion of human bone marrow- derived stromal progenitor cells (mesenchymal ... Chen B, et al Ex vivo expansion and in vivo infusion of bone marrow- derived Flk-1+CD31-CD34- mesenchymal stem cells: feasibility and safety from monkey to human Stem Cells Dev,2006,15(3):349-357...

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Báo cáo khoa học: " Isolation and characterization of canine umbilical cord blood-derived mesenchymal stem cells" pps

Ngày tải lên : 07/08/2014, 23:22

... Anghileri E, Andreini A, Pizzolo G, Sbarbati A, Bonetti B Induction of neural-like differentiation in human mesenchymal stem cells derived from bone marrow, fat, spleen and thymus Bone 2007, 40, ... in the bottom of the tube (Fig 3D), and positive to toluidine blue staining (Figs 4E and F) Discussion Isolation and characterization of stem cells derived from various tissues and sources have ... measured and calculated and drawn as a graph A Fig Identification of the cumulative population doubling level (CPDL) and culture of canine umbilical cord blood (cUCB) -mesenchymal stem cells (MSCs) Cells...

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Báo cáo y học: "Transcriptional profiles discriminate bone marrow-derived and synovium-derived mesenchymal stem cells" ppsx

Ngày tải lên : 09/08/2014, 07:20

... expressed in bone marrow- derived or synovium -derived cells Genes whose mRNA expression is differentially expressed in bone marrow- derived or synovium -derived cells Gene Upregulated in bone marrow- derived ... whereas it was negative Figure derived adherent cells Immunosuppressive properties of bone marrow- derived and synoviumderived adherent cells (a) Proliferative activity of T cells in a mixed lymphocyte ... derived adherent cells In-vitro-induced differentiation of bone marrow- derived and synoviumderived adherent cells (a) Chondrogenic differentiation was evaluated after 21 days in micropellet culture...

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Báo cáo y học: "Biochemical markers of bone turnover and their association with bone marrow lesions" docx

Ngày tải lên : 09/08/2014, 13:21

... knees On bone histology, areas of bone affected by BMLs exhibit reversal lines indicative of bone remodeling [10] Small areas of bone necrosis are also present, as is scar The extensive bone remodeling ... Crutchfield M, Jannausch ML: The associations of bone mineral density and bone turnover markers with osteoarthritis of the hand and knee in pre- and perimenopausal women Arthritis Rheum 1999, ... Heart Study Offspring cohort included surviving descendants (and spouses of descendants) of the original Framingham Heart Study cohort [25] As part of a study of the inheritance of OA, selected...

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Báo cáo y học: " Reconstitution of the myeloid and lymphoid compartments after the transplantation of autologous and genetically modified CD34+ bone marrow cells, following gamma irradiation in cynomolgus macaques" potx

Ngày tải lên : 13/08/2014, 05:21

... gamma-radiation Figure Recovery of bone marrow clonogenic activity Recovery of bone marrow clonogenic activity Bone marrow- derived colony-forming units following sublethal irradiation of cynomolgus monkeys ... Transplantation of modified CD34+ bone marrow cells After the coculture of CD34+ cells with the lentiviral vector, four animals underwent intramedullary infusion, of whole immunoselected CD34+ cells into ... Immunoselection of non human primate CD34+ bone marrow progenitor cells Bone marrow mononuclear cells were obtained from the iliac crest or by aspiration from the humerus and isolated by standard Ficoll...

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Differentiation of bone marrow derived mesenchymal stem cells (BM MSCs) using engineered nanofiber substrates

Ngày tải lên : 11/09/2015, 09:57

... Types of cells used in bone tissue engineering for osteogenic differentiation 59 2.3.1 Potential of mesenchymal stem cells (MSCs) for bone healing 60 2.3.2 Potential of bone marrow derived mesenchymal ... cells: MSCs, osteoprogenitor cells, osteoblasts, chondrocytes Mitogenic for mesenchymal cells and osteoblasts, chemotactic for inflammatory and mesenchymal cells Targeted cells: mesenchymal and ... chondrocytes maturation and bone resorption Targeted cells: mesenchymal and epithelial cells, osteoblasts and chondrocytes IGF-I: mesenchymal and osteoprogenitor cells recruitment and proliferation,...

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Isolation and characterization of an extracellular polymer closely related to flocculation of activated sludge

Ngày tải lên : 05/09/2013, 09:08

... polysaccharide One milligram of the partially purified polymer contained 309.7 µg of protein, 160.2 µg of neutral sugar, 58.5 µg of hexosamine, 30.8 µg of uronic acid and 3.8 µg of phosphorus Constituent ... as follows: 500 g of soil was washed times with distilled water and suspended in 1,000 ml of water The mixture was allowed to stand for 10min and the supernatant was collected and used for the ... concentration of ppm to estimate the effect of pH After min, A660 was measured and the extent of flocculation was calculated Pronase digestion of extracellular polymer Forty milligrams of the extracellular...

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Tài liệu Báo cáo khoa học: Isolation and characterization of four type 2 ribosome inactivating pulchellin isoforms from Abrus pulchellus seeds docx

Ngày tải lên : 18/02/2014, 16:20

... for cDNA single strand synthesis, 600 ng of RNA, 0.5 lg of oligo(dT) primer and 10 mm of dNTPs were incubated for at 65 °C Subsequently, lL of the ﬁrst strand buffer X and lL of dithiothreitol ... mixture of other isoforms (B) Gel visualization of proteins eluted Lane 1, molecular weight markers Numbers on the left indicate the Mr values of the standards in thousands Lanes 2–9, lg of peak ... P III ⁄ P IV and a mixture of other isoforms (*), respectively, in the presence (lanes 2–5) or absence (lanes 6–9) of 2-mercaptoethanol (C) Elution proﬁle of P III and P IV Chromatofocusing chromatography...

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Tài liệu Báo cáo khoa học: Phenol hydroxylase from Acinetobacter radioresistens S13 Isolation and characterization of the regulatory component docx

Ngày tải lên : 21/02/2014, 00:20

... of 50 nmÆmin)1, band-width of 1.0 nm, and a resolution of 0.1 nm in the temperature range between 10 °C and 70 °C, after preincubation for 10 at each temperature Three runs were accumulated and ... of 0.6 lM The data were fitted to Michaelis–Menten curves Squares and dotted line: data and fitting with PHI/PHO ratio of 0.5 Triangles and continuous line: data and fitting with PHI/PHO ratio of ... Asterisks and broken line: data and fitting with PHI/PHO ratio of hypothesis and therefore a direct involvement of PHI in electron transfer This conclusion is consistent with the very low degree of identity...

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Tài liệu Báo cáo khoa học: The isolation and characterization of cytochrome c nitrite reductase subunits (NrfA and NrfH) from Desulfovibrio desulfuricans ATCC 27774 Re-evaluation of the spectroscopic data and redox properties ppt

Ngày tải lên : 21/02/2014, 00:20

... intense band of 61 kDa (NrfA) and a band of weak intensity of 19 kDa (NrfH), conﬁrming its hetero-oligomeric nature (Fig 1, lane 1) However, in the absence of boiling (Fig 1A, lanes and 4) high ... and 4) high molecular mass bands of approximately 110 kDa and > 200 kDa were visible, as well as a faint band at 37 kDa, suggesting the presence of dimers All of the bands stained positively for ... the ﬁrst time the isolation and biochemical characterization of D desulfuricans ATCC 27774 ccNiR subunits The stoichiometry between NrfH and NrfA is discussed The reassessment of previous spectroscopic...

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Preparative isolation and purification of six volatile compounds from essential oil of Curcuma wenyujin using high-performance centrifugal partition chromatography doc

Ngày tải lên : 07/03/2014, 21:20

... total of mg of curdione (1), mg of curcumol (2), 10 mg of germacrone (3), 18 mg of curzerene (4), mg of 1,8-cineole (5) and 17 mg of b-elemene (6) from the essential oil (300 mg) in 2.4 mL of the ... the differences between K2 and K3, and between K4 and K5 in solvent systems (petroleum ether/acetonitrile/acetone 2:1:1 and 4:3:1 v/v/v) are 0.34 and 0.24, and 0.20 and 0.20, respectively, which ... Both of the upper and the lower phases were degassed for 30 by ultrasonication before use Aliquots of 300 mg of the essential oil were diluted by 2.4 mL of the mixed solution of lower phase and...

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