isolation and culture of nscs

báo cáo hóa học:" Isolation and culture of fibroblasts from endoscopic duodenal biopsies of celiac patients" pdf

Ngày tải lên : 18/06/2014, 15:20

... positive for FSP and negative for cytokeratin 20 in all the cultured and examined cells (Figure 3) Discussion Q-banding analysis of FBs from CD and non-CD subjects demonstrated a normal and stable ... endotoxin and exert cytotoxic effects on primary cultures with an increase of lipidic intracellular droplets [29] Liberase is a blend of highly purified enzymes used to improve the isolation and cultures ... majority of affected ones The method of cell culture here described could help in the establishment of novel experiments to study the role of FBs in the pathogenesis of the mucosal damage and to...

8
563
1

Isolation and characterization of an extracellular polymer closely related to flocculation of activated sludge

Ngày tải lên : 05/09/2013, 09:08

... polysaccharide One milligram of the partially purified polymer contained 309.7 µg of protein, 160.2 µg of neutral sugar, 58.5 µg of hexosamine, 30.8 µg of uronic acid and 3.8 µg of phosphorus Constituent ... as follows: 500 g of soil was washed times with distilled water and suspended in 1,000 ml of water The mixture was allowed to stand for 10min and the supernatant was collected and used for the ... concentration of ppm to estimate the effect of pH After min, A660 was measured and the extent of flocculation was calculated Pronase digestion of extracellular polymer Forty milligrams of the extracellular...

12
510
1

Tài liệu Báo cáo khoa học: Isolation and characterization of four type 2 ribosome inactivating pulchellin isoforms from Abrus pulchellus seeds docx

Ngày tải lên : 18/02/2014, 16:20

... for cDNA single strand synthesis, 600 ng of RNA, 0.5 lg of oligo(dT) primer and 10 mm of dNTPs were incubated for at 65 °C Subsequently, lL of the ﬁrst strand buffer X and lL of dithiothreitol ... mixture of other isoforms (B) Gel visualization of proteins eluted Lane 1, molecular weight markers Numbers on the left indicate the Mr values of the standards in thousands Lanes 2–9, lg of peak ... P III ⁄ P IV and a mixture of other isoforms (*), respectively, in the presence (lanes 2–5) or absence (lanes 6–9) of 2-mercaptoethanol (C) Elution proﬁle of P III and P IV Chromatofocusing chromatography...

12
763
0

Tài liệu Báo cáo khoa học: Phenol hydroxylase from Acinetobacter radioresistens S13 Isolation and characterization of the regulatory component docx

Ngày tải lên : 21/02/2014, 00:20

... of 50 nmÆmin)1, band-width of 1.0 nm, and a resolution of 0.1 nm in the temperature range between 10 °C and 70 °C, after preincubation for 10 at each temperature Three runs were accumulated and ... of 0.6 lM The data were fitted to Michaelis–Menten curves Squares and dotted line: data and fitting with PHI/PHO ratio of 0.5 Triangles and continuous line: data and fitting with PHI/PHO ratio of ... Asterisks and broken line: data and fitting with PHI/PHO ratio of hypothesis and therefore a direct involvement of PHI in electron transfer This conclusion is consistent with the very low degree of identity...

7
514
0

Tài liệu Báo cáo khoa học: The isolation and characterization of cytochrome c nitrite reductase subunits (NrfA and NrfH) from Desulfovibrio desulfuricans ATCC 27774 Re-evaluation of the spectroscopic data and redox properties ppt

Ngày tải lên : 21/02/2014, 00:20

... intense band of 61 kDa (NrfA) and a band of weak intensity of 19 kDa (NrfH), conﬁrming its hetero-oligomeric nature (Fig 1, lane 1) However, in the absence of boiling (Fig 1A, lanes and 4) high ... and 4) high molecular mass bands of approximately 110 kDa and > 200 kDa were visible, as well as a faint band at 37 kDa, suggesting the presence of dimers All of the bands stained positively for ... between NrfH and NrfA is discussed The reassessment of previous spectroscopic studies was undertaken, particularly regarding the assignment of spectroscopic and redox potentials of the NrfA hemes...

12
593
0

Preparative isolation and purification of six volatile compounds from essential oil of Curcuma wenyujin using high-performance centrifugal partition chromatography doc

Ngày tải lên : 07/03/2014, 21:20

... total of mg of curdione (1), mg of curcumol (2), 10 mg of germacrone (3), 18 mg of curzerene (4), mg of 1,8-cineole (5) and 17 mg of b-elemene (6) from the essential oil (300 mg) in 2.4 mL of the ... the differences between K2 and K3, and between K4 and K5 in solvent systems (petroleum ether/acetonitrile/acetone 2:1:1 and 4:3:1 v/v/v) are 0.34 and 0.24, and 0.20 and 0.20, respectively, which ... Both of the upper and the lower phases were degassed for 30 by ultrasonication before use Aliquots of 300 mg of the essential oil were diluted by 2.4 mL of the mixed solution of lower phase and...

7
502
0

Báo cáo Y học: Isolation and characterization of a thioredoxin-dependent peroxidase from Chlamydomonas reinhardtii doc

Ngày tải lên : 08/03/2014, 16:20

... BLAST of peptide sequences of 21 kDa proteins in databases Tryptic peptides were puri®ed by RP-HPLC and some of them were totally or partially analyzed by Edman sequencing (ááá) and/ or by MALDI-TOF ... BAS1 protein of Brassica, spinach, barley and A thaliana, PR1 of Phaseolus and MHF of A thaliana These proteins belong to the 2Cys-Prx subfamily All plant 2Cys-Prx proteins, except BAS1 of barley, ... previously [21] Chloroplastic thioredoxins m and f from spinach were a kind gift of P Schurmann (University È of Neuchatel, Switzerland) Thioredoxins m and h from Ã Chlamydomonas were puri®ed as...

11
608
0

Báo cáo khoa học: Isolation and characterization of a D-cysteine desulfhydrase protein from Arabidopsis thaliana pptx

Ngày tải lên : 16/03/2014, 18:20

... presequence of 19 amino acids The mature protein would have a molecular mass of 41.7 kDa and a pI of 6.34 The YedO protein from E coli and the d-CDes from Arabidopsis showed an overall identity of 36% and ... the presence of d-cysteine in the culture medium It was suggested that d-cysteine exerts its toxicity through an inhibition of threonine deaminase On the other hand, the presence of the yedO gene ... d-CDes mRNA and protein levels in older plants, during the light phase in a diurnal light ⁄ dark rhythm and under sulfate limitation Results In silico characterization and isolation of the Arabidopsis...

14
565
0

Báo cáo khoa học: Isolation and characterization of an IgNAR variable domain speciﬁc for the human mitochondrial translocase receptor Tom70 potx

Ngày tải lên : 17/03/2014, 10:20

... respectively, and the resultant VNARs evaluated for retention of binding afﬁnity Material and methods Equipment and reagents Restriction enzymes and Vent DNA polymerase were purchased from New England ... possibility of inter- and intra CDR disulphide cross-links These strategies are summarized in Fig 1B and Table 1, and details of the library construction are given in greater detail in the Materials and ... possibility of disulphide bond formation genetically by replacement of residues Cys22 and Cys82 with alanine and valine, respectively, to give a cysteine minus mutant (12F-11DCys) Use of alanine and...

12
522
0

Báo cáo khoa học:The isolation and characterization of temperature-dependent ricin A chain molecules in Saccharomyces cerevisiae docx

Ngày tải lên : 23/03/2014, 07:20

... permissive temperatures of 30 °C and 37 °C, respectively A sample of the cells was removed from each culture, rRNAs were isolated in TRIzolÒ (Invitrogen, Paisley, Scotland), and the extent to which ... site molecules Y80, Y123 and E177 are shown in green and the position of the two mutated amino acids, F108 and L151, are shown in blue between the aromatic rings of Tyr80 and Tyr123 close to the ... diagram of wild-type RTA structure, and the positions of the altered amino acids of the double mutant, F108 and L151, within the structure are indicated Discussion RTA is the catalytic polypeptide of...

14
411
0

Báo cáo Y học: Isolation and characterization of MUC15, a novel cell membrane-associated mucin pot

Ngày tải lên : 24/03/2014, 04:21

... Switzerland), thermolysin, chymotrypsin or elastase (Sigma, St Louis, MO, USA)] and Cloning of the bovine MUC15 cDNA by PCR with degenerate primers Isolation of total RNA from the mammary gland of ... PCR screening of bovine and human cDNA libraries showed the presence of MUC15 mRNA in human breast, bovine mammary gland, bovine lymph nodes and lungs of both species (Table 1) Of the identiﬁed ... part of the coding sequence was then cloned and sequenced using PCR as described above with 0.4 lM and lM of the speciﬁc P7 primer and degenerate primers (P4-P6), respectively The cDNA sequence of...

9
614
0

the dialects and culture of the kinki region - kinki japanese

Ngày tải lên : 27/03/2014, 12:26

... Asi iN -U 橋 d h ensa oH-O -AYU 篭車一 F-u Uk ル KU ー K-s Utu b-U uS cuUk h a n E anD? k-S uTU B-u Us CUu H - ・ka N e And? 月 R 靴ぶす rn a I cny ad big rde cosik hptcs dg o tI rn a g o mrig o d onn ... a δi ， l s ocsoal，o h u o t i rvr w e h dos o of r o h c r w o ie y u hne f o l e h n e rp y u f o t t l k h gvs o cag.Iy u i e e v rt i ae i Koof g aot aig o o r rgt otd o Jpns c s s n yt ，o ... te ci n a s ses ts i Nvrhls，ery vr aaya ad fie ay n s k ad eetees nal eeyslrmn n ofc ld i O a a n truhu asihsland osek tnad aaee o ue n hogotKna a ere t pa sadr Jpns f s i r bsns，lhuh e r rnnito...

173
444
0

Báo cáo khóa học: Isolation and characterization of the Xenopus HIVEP gene family ppt

Ngày tải lên : 30/03/2014, 13:20

... of degenerate and speciﬁc primers and by rescreening the cDNA libraries The partial clones of XHIVEP2 and -3 covered 5.9 and 3.2 kb of the respective 3¢ ends and included 3¢-UTRs and poly(A)-tails ... upper strands with [32P]ATP[cP] and T4 polynucleotide kinase The labeled oligomers were annealed by heating to 90 °C an equimolar mixture of the upper and lower strands in reaction buffer and cooling ... -2 and -3) The Xenopus XHIVEPs are characterized with respect to temporal and spatial expression, nuclear import/export activity and DNA binding speciﬁcity Materials and methods Isolation and...

10
414
0

temple university press customizing the body the art and culture of tattooing mar 2008

Ngày tải lên : 11/06/2014, 15:14

... (Laumann and Derick, 2006) The movement of tattooing into the realm of popular culture displays certain features of the contemporary culture industry and reveals how fad-like phenomena emerge Culture ... art, prison art, tiki art, anime and manga, pulp art, and tattooing Inspired by the dadaists and surrealists of the 1920s and 1930s, advocates and practitioners of lowbrow art reject the constraints ... provide an account of the changes that have occurred within the artistic and cultural world of tattooing and the larger world of body alteration in the past decade and a half One change and a fairly...

272
698
0

Báo cáo hóa học: " Isolation and characterization of cidofovir resistant vaccinia viruses" pot

Ngày tải lên : 20/06/2014, 01:20

... Domains of E9 and locations of mutations responsible for drug resistance Domains of E9 and locations of mutations responsible for drug resistance The location of the putative exonuclease and polymerase ... streptomycin and non-essential amino acids Vero Cells were obtained from ATCC and were maintained in MEM with Earl's salts and the addition of 10% FBS and standard concentrations of L-glutamine, ... Mapping and marker rescue of recombinant viruses A Map of PCR fragments in the E9L region that were used for marker rescue mapping experiments and reconstruction of resistant viruses B Results of...

8
325
0

Báo cáo hóa học: " Isolation and characterization of a new simian rotavirus, YK-1" docx

Ngày tải lên : 20/06/2014, 01:20

... the G and P serotype specificities of the YK-1 strain, the sequences of the genes encoding both VP7 and VP4 were determined and compared with those of representatives of established G and P serotypes ... infectivity, immunity, and pathogenesis of rotavirus and for evaluating candidate rotavirus vaccines [16,17] Results Rotavirus isolation and characterization High titers of rotavirus antigen measured ... rotavirus RNA segment 11, and (c) schematic diagram of the sequences of gene segment 11 Arrows indicate segment 11 of RRV and YK-1, or rearranged segment 11 of variant 311 The YK-1 and its variant clone...

8
530
0

Báo cáo khoa học: "Isolation and identification of Escherichia coli O157:H7 using different detection methods and molecular determination by multiplex PCR and RAPD" docx

Ngày tải lên : 07/08/2014, 18:21

... strains), C1-6 (strains of Cornell Univ.) and P1-7 (strains from E coli reference center of Pennsylvania State Univ.) b The presence of Stx1 and Stx2 “-” and “+” indicate negative and positive, respectively ... determination of stx1, stx2, eaeA, and hlyA in multiplex PCR assay, (iii) to compare the genetic patterns of Korean isolates and U.S isolates, and (iv) to compare the efficiency among conventional culture ... Switzerland) Once suspicious colonies are identified, confirmation of the isolates as E coli O157:H7 is dependent upon biochemical identification and demonstration of the presence of somatic and...

13
456
0

Báo cáo khoa học: " Isolation and characterization of Streptococcus sp.from diseased flounder (Paralichthys olivaceus )in Jeju Island" ppt

Ngày tải lên : 07/08/2014, 18:21

... eht fo esahp suoeuqa reppu eht no detcelloc saw AND lairetcaB nim rof 000,6 ta degufirtnec dna nim rof sllec lairetcab gniliob yb detcartxe neht saw AND lairetcab ;retaw dellitsid elbuod dezilirets ... 817( rof evitisop ,9~4 senal ;)pb 001,1( rof evitisop ,3 dna senal ;noitcefni laccocotperts rof evitagen ,1 enal ;lortnoc evitagen ,N enal ;)pb 003 ,M4040 ( lortnoc evitisop ,P senal ;reddal AND ... enilav ,esapil rof snoitcaer evitagen dah setalosi llA nispyrtomyhc -α dna esatahpsohp enilakla rof snoitcaer evitisop dah fo setalosi 81 dna esadinimasoculg-β-lyteca -N dna esaretse rof snoitcaer...

6
348
0

Báo cáo khoa học: " Isolation and characterization of canine umbilical cord blood-derived mesenchymal stem cells" pps

Ngày tải lên : 07/08/2014, 23:22

... conditions and basic settings for the application of gene delivery were also investigated Materials and Methods Cell isolation and culture Canine umbilical cord blood (cUCB) and blood of the canine ... in the bottom of the tube (Fig 3D), and positive to toluidine blue staining (Figs 4E and F) Discussion Isolation and characterization of stem cells derived from various tissues and sources have ... the cell number The CPDL was measured and calculated and drawn as a graph A Fig Identification of the cumulative population doubling level (CPDL) and culture of canine umbilical cord blood (cUCB)-mesenchymal...

7
439
0

Báo cáo khoa học: " Isolation and identification of a canine coronavirus strain from giant pandas (Ailuropoda melanoleuca)" ppt

Ngày tải lên : 07/08/2014, 23:22

... Canada) Bacteriology failed to grow organisms in cultures inoculated with liver and spleen after 48 h culture Fig Isolation and culture of giant panda virus (GPV) in Felis catus whole fetus (FCWF) ... logarithm of the highest dilution of serum that could neutralize 100 TCID50 of the virus Total RNA was extracted from the giant pandas’ spleens, infected and non-infected FCWF cells (as positive and ... indicates the cytoblast of an infected FCWF cell The black arrow indicates the inclusion bodies of GPV in the cytoplasm of cells Scale bars = 200 nm Isolation and identification of a canine coronavirus...

3
308
0