... chemical glycosylation of a-CT and for the molecular modelling and molecular dynamics simulations The succinimidyl functionality serves as leaving group during the glycosylation reaction FEBS ... mechanochemical nature of a-CT catalysis A more detailed analysis oftheinfluenceof chemical glycosylation onthe dynamics of a-CT from the theoretical simulations was additionally performed ... structural motions onthe functional properties of proteins Because these calculations are traditionally performed on crystal structures their only drawback is that they not take into consideration...
... performance of HsRXR, LmRXR and the two chimeras, Hs–LmRXR (helix 1–8 of HsRXR and 9–12 of LmRXR) and Lm-HsRXR (helix and two loops on either side of helix of LmRXR were replaced with the corresponding ... at h after addition of ligand and reached peak levels of 19 000-fold induction by 48 h after addition of ligand (Fig 11A) The turn off of reporter activity after withdrawal of ligand is also fast ... as well as in the loops on either side of helix contribute to the improved activity of LmRXR Western blot analysis of proteins isolated from 3T3 cells that were transfected with RXR mutant constructs...
... shown above occurs onthe transcriptional or onthe translational level, the system of combined transcription ⁄ translation was decoupled Theinfluenceonthe transcriptional level was investigated ... vitro transcription assays and for analysis oftheinfluenceof CSPs at the transcriptory level In the latter case the reaction volume was 20 lL, where lL ofthe reaction volume were either 50 mm NaH2PO4, ... Assuming a total soluble protein concentration of 200–300 mgỈmL)1 [21,22], the concentration of CspA in the cytosol can reach concentrations in the millimolar range during cold shock The effects...
... collected to draw conclusions about the interaction of peptides with large unilamellar vesicles (LUV) The fluorescence quantum yield is dependent onthe polarity ofthe microenvironment ofthe ... amounts of dimethylsulfoxide The final concentration of dimethylsulfoxide in the samples through the experiments was at most 1.4% (v ⁄ v) The solubilization of all peptides was improved with mild bath ... cholesterol on POPC LUV may lead to the formation of a liquid-ordered phase A moderate cholesterol content onthe POPC ⁄ cholesterol LUV enables the coexistence of cholesterol-rich (in a liquid-ordered...
... variations in stability, although the more thermophilic class II XIs contain additional prolines and fewer thermally labile asparagine and glutamine residues [26,30] The crystal structures of class ... Mg2+ plus 0.5 mM Co2+ The concentration of soluble protein (% of starting concentration, h) and the thermal transitions are shown as functions ofthe temperature PAGE All samples showed a single ... the residual soluble enzyme conformation on SDS and native Excess heat capacity (kcal/mole·K) K L Epting et al 120 Fig Soluble TNXI concentration during thermal denaturation in the presence of...
... into the wells of a Bioscreen microtitre plate (100 well honeycomb; Life Sciences International) to give an inoculum size of 5.0 · 103 cellsỈml)1 as described previously [5,15] The stated concentrations ... [6] Growth of normal yeast on galactose relative to glucose slightly decreases acetate resistance (Fig 2), yet the strong Pdr12p induction with the growth ofthe GAL1-PDR12 strain on galactose decreases ... genotypes (all of mating type a, and also ura3) were spotted (undiluted; also as : 10 and : 100 serial dilutions) onto pH 4.5 YPD containing the indicated level of organic acid The plates were...
... for the oxidation phase are still faster than the overall rate constant during the steady state As the latter is the complex function of all the internal and intermolecular rate constants, the ... probably other factors Nonetheless, catalytic activity is still one ofthe few characteristics that reflect the actual role ofthe oxidase in the tissues What is that role? The oxidase catalyzes the ... energy for the overall reaction The limitation onthe steady state, catalytic rate, is a reasonably fast reaction associated with ET The inclusion of 56% (w/v) ethylene glycol changes that The individual...
... classification onthe emotional exhaustion or depersonalization subscales relates to higher professional burnout, while the personal accomplishment subscale has an inverse relationship to burnout with lower ... represent the rate per cent to the study's 311 respondents A high classification onthe emotional exhaustion or depersonalization subscales relates to higher professional burnout, while lower personal ... (menial tasks) Onthe other hand, a sizable proportion of residents (44.7%) did feel that they dedicate a considerable amount oftime to their patients' care, yet a notable fraction of these residents...
... in the presence of jitter HNRSr will perform worse, since pulse tails would not always be aligned with the adjacent pulse, and the correlation should be lower The evaluation oftheinfluenceof ... as the introduced shimmer increases Onthe other hand, the summation of tails in one pulse is not completely uncorrelated with the summation of tails in the other For this reason, the estimation ... This pulse amplitude (shimmer) normalization can help in thetime warping ofthe pulses and actually reduces the variance ofthe template in Yumoto’s HNR formula However, it still yields only an...
... degradation point ofthe DVB-H service This corresponds well with our criterion, since we also demand valid MPE tables for 95% ofthetime Only, we will use a period of 40 seconds for reasons explained ... Section 2.3) ofthe DVB-H system, based on %Valid reception The definition of R is based onthe definition of %Valid reception (formula (1); see Section 2.3) To calculate R, a window of 80 samples ... investigation) Secondly, models for networks with multiple transmitters are not yet available, we only have knowledge of path loss models for one transmitter Thirdly, unlike range calculations based on...
... Calculated values of local strain are shown in Table Onthe left axis of Fig 2, local strain values are shown with variation in molar cobalt concentration It can be seen that the crystallinity of doped ... nanoparticles (see Fig 1) is fairly good as the local strain values are smaller when the cobalt Nanoscale Res Lett (2010) 5:323–331 325 Table Cobalt molar concentrations in starting solution, y analysed ... due to the strong interaction of d electrons of Mn2? with s–p electrons ofthe host nanocrystalline Zn Zielinski et al [28] and Seong et al [29] reported that the sp–d exchange interactions in...
... slightly larger than the value of 0.28 C/m2 of tetragonal BTO (the other theoretical value is 0.26 C/m2 [28]), which supports the conclusion that the sharp interfaces suppress the polarization in ... function of uniaxial tensile stress For the ground state superlattice, the calculated spontaneous polarization of 0.29 C/m2 is less than the theoretical value of 0.81 C/m2 of ground state PTO, but slightly ... c11, always decrease with increasing r33, indicating that the superlattice under the loading of uniaxial tensile stress along the c axis becomes soft along the nonpolar axes To illustrate the change...
... normally unoccupied under equilibrium conditions The occupation of states (referred to as state filling) following an ultrafast laser pulse will appear as a reduction in the absorption at the probing ... diameter ofthe SnO2 NWs was found to be uniform along their lengthThe growth ofthe SnO2 NWs occurs via the formation of Au nanoparticles (NPs) from the thin layer of Au and the VLS mechanism ... corresponds to the well known Surface Plasmon Resonance (SÁP.R.) of Au nanoparticles that are required as catalysts for the formation ofthe SnO2 NWs on quartz Figure shows typical time resolved...
... In spite ofthe relatively low specific surface areas presented, the catalysts achieved significant ethanol conversion values at the beginning ofthe test The conversion of ethanol for the SnCe#550 ... annealing temperature of 1,100 °C Onthe other hand, for the samples annealed at temperatures lower than this, only the tetragonal SnO2 phase was observed, suggesting the formation of a solid solution ... range The spectra were collected using Mg Ka radiation and the overall energy resolution was around 0.8 eV The concentration ofthe surface elements was calculated using the system database after...
... functional group consists of a secondary amide attached to the MWCNTs along with a long alkyl chain The long alkyl chain helped in preventing agglomeration and bundling of MWCNTs due to repulsive ... imidization, the amide linkage converted in to imide linkage (cyclization) with the evolution of water molecules The whole scheme of nanocomposites fabrication is illustrated in Fig Nanoscale Res Lett ... show the weight loss in two steps, the initial one before 400 °C is due to decomposition ofthe functional groups, while the other one relates to the oxidation ofthe nanotubes A weight loss of...
... here) confirm the existence of a continuum of states in the spectral range ofthe QD transition energies in our samples Conclusion In conclusion, we have measured the rise and decay dynamics ofthe ... different emission peaks observed in the cw-PL spectrum on a time scale of 45 ps It is clearly visible that the rise times ofthe QD states are nearly identical We have found a rise timeof 5.9 ± 0.2 ... quantified in the right plot of Fig These results clearly indicate that first-excited-state emission is observed even at excitation densities well below the level required for state filling in the ground...
... experimental data for the two families of lignins is probably a result oftheinfluenceof other functional groups in the lignin structure [2] In this particular case [2], the effect ofthe other functional ... expander ofthe skeleton Only a small number of Pb nuclei are distinguished onthe skeleton The NAM surface here is small Hence, it can be concluded that during cycling the ‘‘energetic structure’’ of ... presents the end -of- discharge voltage at the 10th week-cycle as a function ofthe number of weeks Fig shows the dependencies ofthe number of week-cycles (giving the cycle life ofthe battery) on the...
... processes of lead sulphate in sulphuric acid solution The polymer was introduced in the paste for negative plate and in the cell electrolyte The effect of PASP onthe performance ofthe negative plate ... recrystallization process of PbSO4 and, probably, will prevent the formation of large PbSO4 crystals during partialstate -of- charge operation 3.2 Influenceof PASP additive onthe capacity ofthe negative ... established with SEM at the end of HRPSoC tests Large polyhedral crystals of PbSO4 can be observed onthe surface ofthe negative plate from R-cell (Fig 8a) The addition of PASP to the electrolyte...
... 0.12 g /L and 0.28 g /L, respectively The application of soluble fertiliser started one month after seedling germination (May) and it was stopped six months later at the end ofthe growing season (November) ... (November) Fertilisation with slow-release fertiliser (SRF) consisted ofthe application of 2.3 g /L substrate of Osmocote Plus (Scotts, Marysville, USA) 15-8-11 (totally released after 12 months, at ... irrespectively ofthe fertilisation method used (Tabs III and IV) DISCUSSION Laccaria laccata effectively colonised almost all seedlings and the formation of ECM was not affected by the fertilisation method...
... Observed results depended onthe connectedness level but also onthe replacement rate ofthe females (each year, 1/5 ofthe females was replaced) At the beginning ofthe simulation, the direct ... reliable solution could be the use of DNA markers The conditions for successful DNA fingerprinting depend not only onthe cost ofthe method, but also onthe breeding system ofthe animals (number of ... assess the relative importance of deviations from the ideal situation The second purpose of this article is to test, by simulation, theinfluenceof data structure onthe estimation of genetic parameters...