immortalization of human b cells analysis of b cell repertoire and production of human monoclonal antibodies

Báo cáo y học: " Generation and characterization of high affinity human monoclonal antibodies that neutralize staphylococcal enterotoxin B" ppsx

Ngày tải lên : 11/08/2014, 08:21

... generation and selection of fully human monoclonal antibodies (HuMAbs) specific for Page of SEB derived from human B- cell hybridomas These antibodies showed biological activity towards SEB in vitro ... individuals with anti-SEB serum antibody titers B cells were isolated from the PBMC population using an EasySep human B cell enrichment kit (StemCell Technologies) B cells were cultured for days ... blood mononuclear cells (PBMCs) were used to determine the ability of HuMAbs to inhibit SEB-induced T -cell cytokine production and measure their in vitro EC50 PBMCs isolated from blood drawn from...

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Báo cáo y học: " Reconstitution of the adult B cell repertoire after treatment with rituximab" pptx

Ngày tải lên : 09/08/2014, 07:20

... immature/transitional B cells, and that was more in accord with the levels expressed by memory cells An explanation for this could be that these cells represent a subset of memory cells lacking CD27, ... profound B cell depletion there could be a re-enactment of early B cell ontogeny and that the cells described in the report by Rouzière and coworkers [1] might represent mutated marginal zone B cell ... the interplay and competition between these cells and newly generated B cells The growing availability of patients treated with B cell depletion should allow investigators to understand the determinants...

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Báo cáo y học: " Importance of spliceosomal RNP1 motif for intermolecular T-B cell spreading and tolerance restoration in lupus" pptx

Ngày tải lên : 09/08/2014, 10:21

... activate B cells to produce anti-RNP1 antibodies (Abs) These B cells then bind and process the RNP1 epitope present within RNP1+ proteins, such as U1-70K (in blue) and U1-A (in green), but also ... 131–151 peptide, we found no antibodies to dsDNA or chromatin, no antibodies to recombinant SmBB', and no clinical sign of autoimmunity Immunization of experimental rabbits and mouse models with the ... RNP1-proteins, such as U1-C (in yellow) and SmD1 (in pink) proteins This leads to the activation of Th and B cells and results in the production of diverse sets of auto -antibodies, which then deposit in...

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Báo cáo y học: "The critical role of arginine residues in the binding of human monoclonal antibodies to cardiolipin" ppsx

Ngày tải lên : 09/08/2014, 06:22

... 41–96 B3 IS4 UK4 IU 89–376 B3 B3 B3 B3 3.5–6 B3 B3 IS4 BI 120–608 B3 B3 UK4 BU 40–68 B3 UK4 UK4 UK4 8–28 B3 UK4 IS4 UI 60–480 B3 UK4 B3 UB 2–20 IS4 B3 (Arg27aSer) B3 B3 a 48–60 B3 B3 (Arg27aSer) B3 B3 a ... one of the human monoclonal IgG antibodies IS4, B3 or UK4 and the CDR2 and CDR3 of a different one of these antibodies Two hybrid VL chains (BU and UB) had previously been made by Dr Haley and ... IS4 74–80 IS4VHi B3 B3 B3 24–54 IS4VHii B3 B3 B3 30 IS4VHiii B3 B3 B3 30–34 IS4VHiv B3 B3 B3 28–30 IS4VHx B3 B3 B3 32–34 IS4VHi&ii B3 B3 B3 32–47 IgG concentrations in COS-7 cell supernatants...

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GENERATION AND CHARACTERIZATION OF HUMAN MONOCLONAL ANTIBODIES WITH NEUTRALIZING ACTIVITY FOR DENGUE VIRUS

Ngày tải lên : 09/09/2015, 08:16

... 2004) Besides using EBV to immortalize human B cells, an alternative approach to obtain human B cell lines as a source of human mAbs involve the expression of B cell lymphoma-6 (Bcl-6) and BCL-xL ... frequency of antigen-specific cells and eliminates the need for extensive subcloning Another group combined EBV immortalization of human B cells with hybridoma-based technology to obtain human hybridomas ... antibody-secreting cells These B cells that secrete antibodies can be further separated into short-lived plasmablasts and longlived plasma cells Both memory and antibody-secreting B cells have been utilized in...

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The generation of native human monoclonal antibodies with neutralising activity for dengue virus 1

Ngày tải lên : 11/09/2015, 09:56

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The generation of native human monoclonal antibodies with neutralising activity for dengue virus 2

Ngày tải lên : 11/09/2015, 09:56

... of transformation of B cells with EBV 94 3.2 Optimization of cloning of EBV immortalized B cells 95 3.3 Markers of immortalized B cells 97 3.4 Heat-killed DV neither enhanced B cell growth nor ... Sequences of IgG genes extracted from EBV-infected B cells suggest reactivation of SHM 5.3 Upregulation of AID in EBV infected B cells 117 119 Chapter – Production of neutralizing recombinant human monoclonal ... blot of 14C10 mAb binds to E protein of DV1 Figure 7.10 Western blot and native gel of 14C10 mAb against DIII of E protein of DV 138 139 xiii Figure 7.11 Graph of binding affinities of 14C10 Fab,...

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The generation of native human monoclonal antibodies with neutralising activity for dengue virus 3

Ngày tải lên : 11/09/2015, 09:56

... example, up to 50 % of all CD8+ cells in the blood are cytotoxic T cells that are against cells replicating EBV The major targets for these cells are most probably memory B cells which have initiated ... attachment of EBV to B cells EBNA-LP is encoded by the leader of each of the EBNA mRNAs Molecular genetic analysis indicates that although not required for B cell immortalization in vitro, EBNA-LP ... engagement of the lytic or latent program of the virus The proteins are six EBV nuclear antigens (EBNA1, EBNA2, EBNA3A, EBNA 3B, EBNA3C) and EBNA leader protein (EBNA-LP)) and three latent membrane...

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Báo cáo khoa học: "The use of crossreactive monoclonal antibodies to characterize the immune system of the water buffalo (Bubalus bubalis)" pdf

Ngày tải lên : 07/08/2014, 15:20

... subset WC1-N3 subset cluster 105 106 W C Davis et al Table Continued mAb Ig isotype Specificity CACTB7A CACTB15A BAQ76A BAQ89A BAQ159A BAS2A BAS3A BAS9A BAQ44A BAQ155A LCTB16A PIG45A2 BIG73A BIG501E ... BIG73A BIG501E BIG43A RH1A TH2A B1 8A MM12A MM13A BAQ153A BAQ30A BAT75A FW4-101 BAG40A BAT31A CACTB51A GB58A CAPP2A GB84A 218 BAQ92A LCTB19A LCTB39A TH1A TH18A CH138A MM20A DH5 9B TH17A BAGB27A ANA8A ... BAT18A BAT42A BAT76A LCTB36A MM1A CACT9 3B CACT138A GC50A ILA11A CC17 BAQ82A BAQ91A CACT141A BAQ111A TH82D1 CACT80C CACT88C 7C 2B ST8 GB21A CACT18A CACT19A GB22A CACTB6A CACTB14A CACTB81A CACTB44A...

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Báo cáo y học: " Anti-T cell immunoglobulin and mucin domain-2 monoclonal antibody exacerbates collageninduced arthritis by stimulating B cels" pdf

Ngày tải lên : 12/08/2014, 15:22

... influences both proliferation and antibody production of B cells during the early phase of CIA, but not induction of Th1 or Th17 cells Materials and methods Animals and cells Male DBA/1 mice and female ... mAbs not inhibit H-ferritin binding to activated B cells Purified splenic B cells were stimulated with the combination of anti-IgM, anti-CD40 monoclonal antibody (mAb), and IL-4 for 72 hours Cells ... Expression of TIM-2 on CD4 T and B cells (a) Expression of T cell immunoglobulin and mucin domain (TIM)-2 on activated CD4 T cells Purified splenic CD4 T cells were stimulated by immobilized anti-CD3 monoclonal...

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Báo cáo y học: "CD23+/CD21hi B-cell translocation and ipsilateral lymph node collapse is associated with asymmetric arthritic flare in TNF-Tg mice" potx

Ngày tải lên : 12/08/2014, 17:22

... follicular B cells (Fo), the marginal zone B cells (MZ), and the B- in population The percentage of each population is shown The percentage of B- in cells within this B2 20+/IgM+ fraction from PLN (C), and ... expanding and collapsed PLN, we investigated these features in ipsilateral PLN and ILN Tissue sections were immunostained for both B cells and lymphatic endothelial cells with labeled antibodies ... antibody FITC-anti-rabbit IgG (Invitrogen, Carlsbad, CA, USA) or PE-anti-rabbit IgG (Invitrogen) To assess colocalization of B cells within lymphatic vessels, IHC photographs were taken and then...

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Tài liệu Báo cáo khoa học: Constitutive oligomerization of human D2 dopamine receptors expressed in Spodoptera frugiperda 9 (Sf9 ) and in HEK293 cells Analysis using co-immunoprecipitation and time-resolved ﬂuorescence resonance energy transfer pdf

Ngày tải lên : 21/02/2014, 00:20

... plasmid pBlueBac4.5 (Invitrogen), to produce the recombinant plasmids pBBHD2L and pBBHD2S These plasmids were then co-transfected with Bac-N-BlueTM DNA (Invitrogen) in Sf9 insect cells, and underwent ... after exposure of the membranes to Hybond-ECL X-ray ﬁlm (Amersham) Binding of Eu3+ chelate-labelled antibodies Experiments were conducted using whole Sf9 and HEK293 cells or by using membranes prepared ... Eu3+-labelled anti-FLAG Ig and XL665-labelled anti-FLAG Ig (CIS bio international) antibodies (2.5 nM each) The experiments were conducted as described above for whole cells and cell membranes...

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Báo cáo sinh học: " Spectratyping analysis of the islet-reactive T cell repertoire in diabetic NOD Igμnull mice after polyclonal B cell reconstitution" potx

Ngày tải lên : 18/06/2014, 19:20

... polyclonal profile of induced clonotypes, with BV2, BV10, BV12 and BV14 being present on over 60% of the animals, followed next in appearance by BV8S3 and BV11, present in 50% of the mice B) As disease ... mice reconstituted with B cells (white bars), and diabetic NOD.Igμnull mice reconstituted with B cells (grey bars) subsets of T cells, in comparison to age-matched B- cell reconstituted NOD.Igμ ... number of CD19+ B cells circulating in blood post-reconstitution in NOD.Igμnull mice varied from to 13%, with no correlation of higher blood B cells and diabetes onset (data not shown) B cell...

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o cáo hóa học:" Spectratyping analysis of the islet-reactive T cell repertoire in diabetic NOD Igμnull mice after polyclonal B cell reconstitution" docx

Ngày tải lên : 20/06/2014, 04:20

... polyclonal profile of induced clonotypes, with BV2, BV10, BV12 and BV14 being present on over 60% of the animals, followed next in appearance by BV8S3 and BV11, present in 50% of the mice B) As disease ... mice reconstituted with B cells (white bars), and diabetic NOD.Igμnull mice reconstituted with B cells (grey bars) subsets of T cells, in comparison to age-matched B- cell reconstituted NOD.Igμ ... number of CD19+ B cells circulating in blood post-reconstitution in NOD.Igμnull mice varied from to 13%, with no correlation of higher blood B cells and diabetes onset (data not shown) B cell...

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Báo cáo y học: "Differential expression of chemokine receptors on peripheral blood B cells from patients with rheumatoid arthritis and systemic lupus erythematosus" ppsx

Ngày tải lên : 09/08/2014, 06:23

... total B cells (b) (***) naive B cells (**) (**) (**) 60,4 % CD20 75 75 50 memory B cells 50 25 naive B cells cell frequency [%] CD27 38,4 % plasma cells 100 25 1,2% 100 OA RA SLE memory B cells ... on B cell subpopulations The percentage of total B cells, naive B cells, memory B cells and plasma cells subpopulations expressing CXCR5+ are given Box plots show the median values, 25th and ... The percentage of CXCR3hi and CXCR5+ B cells is shown for total B cells and the subpopulation of naive B cells Box plots show the median values, 25th and 75th quartile and the range of values Significant...

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Báo cáo y học: "B cell-activating factor of the tumor necrosis factor family (BAFF) is expressed under stimulation by interferon in salivary gland epithelial cells in primary Sjögren''''s syndrome" ppt

Ngày tải lên : 09/08/2014, 07:20

... Immunocytochemical analysis of salivary epithelial cells Cells were obtained from minor salivary glands (a–e) and the HSG cell line (f–i) Positive cells staining for cytokeratin (a) and cytokeratin 19 (b) and ... whether soluble forms of BAFF could be secreted by epithelial cells At baseline and after stimulation with IL-10 and with TNF-α, ELISA detected no soluble BAFF in cells from patients with pSS and from ... excluded the possibility of contamination with myeloid cells, B cells, T cells or myoepithelial cells (Figure 2d–h) The HSG cell line had exactly the same staining pattern as epithelial cells from patients...

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Báo cáo y học: "Rituximab therapy reduces activated B cells in both the peripheral blood and bone marrow of patients with rheumatoid arthritis: depletion of memory B cells correlates with clinical response" potx

Ngày tải lên : 09/08/2014, 14:22

... HLA-DR+ B cells both in the PB and the BM of RA patients Favorable response to rituximab therapy preceded by a decrease in CD19+CD27+ memory B cells in peripheral blood and bone marrow of RA patients ... cytometry analysis of PB CD19+ B cells in a RA patient at baseline (left) and after 12 weeks of rituximab treatment (right) (c) Rituximab depletes activated CD19+HLA-DR+ B cells both in the PB and ... population of memory B cells both in the PB and in the BM of RA patients Depletion of total and activated B cells is a specific effect of rituximab therapy To test whether B- cell depletion is specific...

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Báo cáo y học: "Synergistic chondroprotective effects of curcumin and resveratrol in human articular chondrocytes: inhibition of IL-1β-induced NF-κB-mediated inflammation and apoptosis" potx

Ngày tải lên : 09/08/2014, 14:22

... RG: Stimulation by human interleukin of cartilage breakdown and production of collagenase and proteoglycanase by human chondrocytes but not by human osteoblasts in vitro Biochim Biophys Acta 1984, ... activation of NF- B is the phosphorylation and degradation of I B , the natural blocker of NF- B [53,54] To test whether inhibition of IL-1β-induced NF- B activation occurs through inhibition of I B ... Singh S, Myers JN, Aggarwal BB: Inhibition of growth and survival of human head and neck squamous cell carcinoma cells by curcumin via modulation of nuclear factor-kappaB signaling Int J Cancer...

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báo cáo khoa học: "eneration of diffuse large B cell lymphomaassociated antigen-specific Va6/Vb13+T cells by TCR gene transfer" pdf

Ngày tải lên : 10/08/2014, 22:21

... CD3+T cells from PB of healthy individuals stained with TCR Vb13-specific antibody and imaged by LSM (×630) A) CD3+T cells transduced with Vb13-IRES-TCR Va6 recombinant plasmid and stained by FITC-TCR ... vector bIa yielded a better function of human antigen-specific TCR-redirected T cells than did vector aIb Cytotoxicity of TCR gene-transduced CD3+T cells against Toledo cells by two types of TCR ... in human CD3+T cells TCR gene-transduced CD3+T cells were stained with a TCR Vb13-specific antibody and analyzed by flow cytometry The numbers indicate the percentage of TCR Vb13-positive cells...

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Báo cáo y học: " Expression of parathyroid hormone-related protein during immortalization of human peripheral blood mononuclear cells by HTLV-1: Implications for transformation" pps

Ngày tải lên : 13/08/2014, 05:20

... assays of PBMCs from healthy human donors with irradiated HTLV-1 producer cells (SLB-1) in the presence or absence of IL-2 Viable cells were counted by trypan blue exclusion and the results are shown ... presence of IL-2 (A), in the absence of IL-2 (B) and for MT-2 cells (C) The data showed that PTHrP was up-regulated in PBMCs following HTLV-1 infection by the activation of both the P2 and P3 promoters ... Up-regulation of PTH1R in PBMCs at weeks 1, 3, 5, 7, 9, 11, and 13 following HTLV-1 infection in the presence or absence of IL-2 compared to day 0; controls and are PBMC-1 and PBMC-2; controls and are PBMC-1...

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