... type-3
coppers) is responsible ofthe adsorption of light at 330 nm.
The T1 c opper domain i s the primary electron acceptor
from the reducing substrate and electrons are transferred
from this copper to the ... activity (%)
pH
Fig. 8. Effect ofthe pH on the activity ofthe purified laccase. pH in the
range of 2.5–8 were tested with 500 l
M
ABTS (d )and1 7l
M
of
syringaldazine ( j) as the su bstrate.
Sd 1
94 ... ammonium
sulfate, and a concentration b y ultrafiltration followed by a
Mono Q column. All the characteristics ofthe recombinant
laccase are in agreement with those ofthe native laccase. This
is the first...
... conclusion is consistent with the very
low degree of identity between the N-terminal sequence of
PHI and those of ferredoxin-like proteins belonging to other
oxygenases. On the contrary, the N-terminus ... possibility is that PHI interacts with one
PHR and one PHO (abc) protomer. The hypothesis of a
direct PHI–phenol interaction is quite unlikely, because of
the fact that the addition of phenol does ... operating in the case of PH from A. radioresistens S13,
as shown by the lack of alteration in the PHO-ANS
fluorescence upon addition of PHI.
In summary, while the regulatory components of MMOs
act...
... avor the displacement ofthe equilibrium
toward the neutral state of His290.
To attest this hypothesis, an Arg213Ala mutant has been
expressed and characterized. The specific activity of the
Ala213 ... alcoholate. The shape of
the titration curve ofthe catalytic histidine shows an
apparent pKa value ofthe essential histidine estimated to
4.1 (Fig. 2C). This acidic pKa shift ofthe histidine ... Indeed, the
presence of Arg213 along with the proximity ofthe charge
relay Asp260 within the active site could be responsible for
the modification ofthe catalytic properties ofthe catalytic
histidine....
... transcription and translation of the
reporter gene. Alternatively the cells were left in the presence
of the antibody for t he complete length of this period.
In both cases the result was the same. As shown ... those of CD53, CD9
or CD37 [15]. However, despite the knowledge of some of
the eff ects induced by tet raspanin proteins andthe proteins
with which they interact, the identification ofthe s ... the top is a gel
from one experiment, showing incorporation of phosphate detected
with the PY99 mA b andthe total amount of Vav protein det ected by
Western blot. The ratio ofthe PY99 to the Vav...
... resolutions ofthe VIth andthe VIIth congresses ofthe Communists Party and
those ofthe important meeting ofthe National Assembly, the Government andthe Central
Party Committee emphasized the importance ... turn
improve the lives of individuals, their families and society as a whole.
The attainment of this objective requires the improvement ofthe educational and
professional level of human resources, ... planning and
management with expertise in both theoretical and practical aspects of their field is a key
issue for accelerating the renovation ofthe education system in Vietnam. This core group of
specialists...
... understand
the strategic position ofthe organization. There is strategic choice which is to do with the
formulation of possible courses of action, their evaluation andthe choice between them.
Finally ... mechanical
85
Chapter 4
Internal Analysis
The final goal of this chapter is that, through the deep analysis of Hanoi Mechanical
Company to reveal the strengths and weaknesses ofthe company in order to find ... non-
synchronous and understandardized products, high production costs and faced the
rejection ofthe market. All of these attributes are differentfrom conditions for the
development of Machinery...
... be
proposed on the basis ofthe results presented here.
Two ofthe mutants (D217E and E221Q) showed no
activity in any ofthe assays. These mutants (and wild-
type) were assessed for their ability ... each of these in order to dissect their contributions to the
isomerase and reductase activities. Several mutations result in loss of the
isomerase activity with retention of reductase activity. However, ... aeruginosa KARI [11]. The tertiary and
quaternary organization of this enzyme is substantially
different from that ofthe spinach enzyme with the
active site constructed from two monomers of a dode-
camer....
... coffee at the price it is now? I guess she doesn’t,”
said her father. He poured the coffee-pot full of boiling water from
the tea-kettle, then he tipped the coffee canister into his hand, and ... that isthe coffee canister,” said
she, pointing.
“Why didn’t you say so, then?” asked her father, viciously, and
again Maria made no reply. Her father seized the coffee canister and
By the ... softly, and crept across the hall to the spare
room, which Aunt Maria had occupied ever since she had been
there. She listened, and heard a soft sob. Then she turned the knob of
the door softly....
... the intracellular segment ofthe b
1
subunit is
required for the interaction with the a subunit, probably a
crucial step for the regulation of channel properties.
The tissue distribution ofthe ... where G
max
is the maximum
conductance, z isthe valence ofthe process and V
ẵ
is the
midpoint voltage of activation.
Ionic current expression levels were determined from
batches of oocytes injected ... the
N-terminal sequence ofthe human b
1
. However, the
C-terminal sequence was significantly differentfrom that
of the human b
1
, suggesting that it might encode a novel
splice variant of the...
... Characterization ofthe TRH-DE from serum and liver (Eur. J. Biochem. 269) 1281
Western blot analysis. To verify the hypothesis that the
brain T RH-DE, the serum TRH-DE andthe liver TRH-DE
arederivedfromthesamegene,theenzymepreparations
were ... is very similar or
identical. These results s trongly support the hypothesis that
the serum TRH-DE andthe membrane-bound TRH-DE
from brain and liver are derived fromthe same gene,
whereby the ... between the particulate liver enzyme
and the serum enzyme were noticed [9,15].
To verify the hypothesis that the serum TRH-DE is of
liver origin we analyzed the TRH-DE i n serum and
tissue ho...
... FSW and improve knowledge and pratising STD
managing for health workers, base on that to expand this intervention model
for other centers over the nation.
4. The layout ofthe thesis
The thesis ... characteristics of FSW is
the nature mobility, due to the inferiority of their career, they do not want to
live in a place fixed for a long time. On the other hand they also have to
move from ...
LRTIs in FSWs. The results of some other domestic and foreign studies
were comformed with the results of this study to point out the high rate of
LRTIs.
Reported by WHO, the incidence of sexually...