e carr b 2006 method for production of chitin and chitosan international bureau wo 2006 124779

Báo cáo khoa hoc:" Quantitative competitive reverse transcription polymerase chain reaction is not a useful method for quantification of CD4 and CD8 cell status during HIV infection" pptx

Ngày tải lên : 11/08/2014, 08:20

... Your research papers will be: available free of charge to the entire biomedical community peer reviewed and published immediately upon acceptance cited in PubMed and archived on PubMed Central ... counts and mRNA levels of CD4 and CD8 could be determined by QC-RT-PCR There was also no correlation between CD4 and CD8 mRNA levels and the number of cells expressing these proteins on their surface ... with BbsI, an enzyme with no recognition sites inside the vector and only one site in the insert The linearized plasmid was gel purified, the free ends of the plasmid were then shortened using Bal31...

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báo cáo khoa học: " Surface electromyography as a screening method for evaluation of dysphagia and odynophagia" ppt

Ngày tải lên : 11/08/2014, 20:20

... needle electrode examination EMG [37] Needle electrodes should be used within the neck area with great precaution and this procedure will be neither time-saving, nor noninvasive The needle EMG ... combination of the time needed for preparation of a patient, time needed for preparation of an instrument, actual time of a procedure, and the time needed for data interpretation and report The timing ... electrode below the lower lip (OO-location); (2) Two electrodes are to be placed parallel to the masseter muscle fibers on the left or right side of the face, preferably on the opposite side from the...

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báo cáo khoa học: " A simple and high-sensitivity method for analysis of ubiquitination and polyubiquitination based on wheat cell-free protein synthesis" potx

Ngày tải lên : 12/08/2014, 03:20

... purified recombinant E2 was detected by luminescent analysis in the presence (E1 +) or absence (E1 -) of exogenous E1 Error bars represent standard deviations from three independent experiments http://www.biomedcentral.com/1471-2229/9/39 ... anti-FLAG and antiHis antibodies, respectively Error bars represent standard deviations from three independent experiments Analysis of the Wheat Cell-free Based Ubiquitination in the Presence of Proteasome ... ATP-dependent manner by forming a high-energy thioester-bond between the carboxyl-terminal glycine residue of ubiquitin and a cysteine residue of E1 The activated ubiquitin is then transferred to...

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05 bacterial cultivation for production of proteins and other biological products (1)

Ngày tải lên : 24/04/2016, 10:44

... probes required are those for pH and DO (iii) Medium preparation: the medium is usually composed of heat-stable and heat-sensitive reagents (see section 10.2.4) The heat-stable reagents can be ... Sometimes, it is not possible to heat-sterilize certain reagents together, and there is a need to separately prepare concentrated solutions of those heat-stable ingredients and to heat-sterilize ... the outlet air of the bioreactor For calculation of specific rates, the convective flow of oxygen and carbon dioxide can be neglected and the transfer rates OTR and CTR can be considered to be...

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Optimization of chitin and chitosan extraction from by product from white leg shrimp (penaeus vannamei) industry in vietnam to improve its quality and efficiency

Ngày tải lên : 15/08/2015, 22:14

... enzymes in shrimp head, especially proteases These proteases include both endoprotease and exoprotease and their activities were equal to commercial proteases however they were easy to be lost due ... Chemical extraction were used prevalently while the procedures which were combined between chemical and biological methods have been applied in a quite limitative level HCl and NaOH were the ... long time (interval of to hours) at ambient temperature in waste house The by-products were often deteriorated seriously before transferring to the place where fishmeal and chitin were produced This...

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Extraction and characterization of chitin and chitosan from marine sources in Arabian Gulf

Ngày tải lên : 25/08/2016, 11:21

... A number of precise and sensitive methods have been derived to achieve the quantitative determination of chitosan and its degree of deacetylation (DDA) Among them, is the dye adsorption method ... acetylated, free amino groups are also present to some extent because of deacetylation during deproteinization process in the alkaline medium Therefore, chitin samples have different degrees of acetylation ... crustacean is not the same for each species, hence studied chitin resources not need the same treatments All species except for cuttleﬁsh were treated with 0.25 M HCl solution at ambient temperature...

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Functional Characterization of Chitin and Chitosan

Ngày tải lên : 25/08/2016, 11:35

... The effect of the degree of deacetylation was greater than the effect of Mw because of its effect on the zeta potential of the nanoparticles However, the uptake of chitosan molecules was less ... polyelectrolyte complexes can be prepared by different methodologies Among them, the one-step procedure and two-step procedures have been used to encapsulate proteins, mainly with the purpose of ... microparticles, as indicated by the particle-size analysis studies Therefore, the exposed surface area is reduced and the release of the entrapped drug is decreased 6.3 Gene Delivery Due to its positive...

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Extraction and characterization of chitin and chitosan from nigerian shrimps ISA 2012

Ngày tải lên : 30/08/2017, 16:30

... be done to improve on the degree of deacetylation probably through size reduction of the chitin, increase in concentrations of the reagents, reaction time and increase in temperature of deacetylation ... were burned in a furnace at temperature of 555 oC and weighed to determine the ash content The fibre and protein content were determined by standard method (AOAC, 1990) RESULTS Percentage composition ... machine The prepared samples were prepared and held on a sample holder and beams of electron passed through The intensity was measured at Bragg’s 2θ angle The Crystallinity of the chitin and chitosan...

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Extraction and characterization of chitin and chitosan limam et al

Ngày tải lên : 30/08/2017, 16:30

... Gram-negative and Gram-positive bacteria This study has been conducted to assess inhibitory effects of chitosan in terms of MIC The effectiveness of chitosan bacteriastatic properties were tested ... the by-products released from seafood processing because of its richness in compounds of high value added such as chitin products Therefore, chitin and chitosan were extracted from Parapenaeus ... than chitin The exact mechanism of the antimicrobial action of chitin, chitosan, and their derivatives is still unknown, but different mechanisms have been proposed Interaction between positively...

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Development of the Quantitative PCR Method for Candidatus ‘Accumulibacter phosphatis’ and Its Application to Activated Sludge

Ngày tải lên : 05/09/2013, 09:38

... were designed for quantitative PCR as shown in Table Sequence of PAO651f is the reverse complement sequence of PAO651 probe Sequence of PAO846r is the same sequence of PAO846 probe For the quantitative ... (EUB338, EUB338 II and EUB338 III) probe (Table 1) The targets of PAOMIX and EUBMIX probe are Candidatus ‘Accumulibacter phosphatis and most eubacteria, respectively The activated sludge samples ... successively for each The hybridization of oligonucleotide probes was performed as described by Amann et al (1995) The slides were observed under a fluorescence microscope (BX-51, Olympus) equipped...

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a rapid method for estiminating of noise expouse workplace

Ngày tải lên : 05/09/2013, 13:23

... 2: Relation between mean SPL values and noise ranking number in study workp laces Table 3: The general representation of the screening matrix Measurement by sound level meter Screening by the noise ... reliance before of noise measuring without necessity to use the instrument in workplaces Therefore, this method is a proper rapid method for exploiting and reducing the expenses by separation of workplaces ... R et al: A Rapid Method for Screening checklist for estimati ng of noise exposure Work place Name: Number of worker: producti on: Main Work place code: Date: Name of screener: Effecti ve items...

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Tài liệu Báo cáo khoa học: "An Ensemble Method for Selection of High Quality Parses" pdf

Ngày tải lên : 20/02/2014, 12:20

... to an ensemble of bagged models to increase parser performance, and (Becker and Osborne, 2005) suggested an active learning technique in which the agreement among an ensemble of bagged parsers ... are not smaller than T We refer to these sentences as T-sentences The ﬁrst measure is the average f-score of the parses of T-sentences Note that we compute the f-score of each of the selected ... set and the baseline sets increases as well The graphs on the right show the number of sentences in the sets selected by SEPA for each T value As expected, this number decreases as T increases...

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Tài liệu Báo cáo khoa học: Molecular characterization and allergenic activity of Lyc e 2 (b-fructofuranosidase), a glycosylated allergen of tomato pdf

Ngày tải lên : 21/02/2014, 00:20

... induce the mediator release even at a high concentration of 10 lgỈmL)1 The CD spectra of the natural and the recombinant Lyc e proteins were highly superimposed and revealed the existence of secondary ... histamine release experiments; to date our results give strong evidence for the allergenic activity of the carbohydrates in a subgroup of tomato allergic patients Therefore the presence of highly ... essential event of the type I allergenic reaction, i .e speciﬁc degranulation of basophils The protein backbone of the allergen does not seem to play a role because the recombinant protein did not...

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Báo cáo khoa học: Degradation of chitosans with chitinase B from Serratia marcescens Production of chito-oligosaccharides and insight into enzyme processivity docx

Ngày tải lên : 07/03/2014, 16:20

... are performed after the enzyme-substrate complex has been formed The latter two mechanisms are often referred to as ‘processive’ Carbohydrate degrading enzymes with tunnel-like active site clefts ... the longer initial products would be equally divided between odd-numbered and even-numbered oligomers The enzyme does not have more than three subsites ()3, )2 and )1) on the glycon side of the ... or even-numbered oligomer, respectively If the enzyme would act processively, all subsequent products coming out of the same binding event will be even-numbered regardless of the initial binding...

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Báo cáo khoa học: "A Nonparametric Method for Extraction of Candidate Phrasal Terms" docx

Ngày tải lên : 08/03/2014, 04:22

... the number of these candidate terms Table Some Lexical Association Measures the overlap between WordNet and the Lexile corpus A list of 53,764 two-word phrases were extracted from WordNet, and ... further pattern (of meaning and usage and thus heightened frequency) superimposed on the normal linguistic base There are, of course, entirely aberrant phrasal terms, but they constitute the exception ... needs to be made must apply in principle to the entire set of word sequences that must be distinguished from phrasal terms, including longer sequences, subsequences, and overlapping sequences,...

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Báo cáo khoa học: "Project for production of closed-caption TV programs for the hearing impaired" docx

Ngày tải lên : 08/03/2014, 06:20

... most of the TV news programs today, the scripts (written text) are available electronically before they are read out by newscasters Japanese news texts are read at the speed of between 350 and ... in a sentence using Japanese language-specific rules 3.1.1 1) Shotening and deletion of sentence ends We find some of phrases which come at the end of the sentence can be shortened or deleted If ... training and selected for the evaluation of the model We used the speech of four people for the evaluation The evaluation results are shown in Table They are the accuracy (detection rate) and false...

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Estimation method for contribution of emission to trace metal concentrations in urban atmosphere based o

Ngày tải lên : 16/03/2014, 00:07

... of the National Air Surveillance Network (from literature) ＊ Mean Value at Refuse Incineration Plants (from literature) The ratios of the Pb and Cd concentrations in the atmosphere obtained by ... from Refuse Incineration (n = 7, measured) While the lead concentration in the atmosphere substantially fluctuates due to different meteorological conditions, the lead isotope ratios in the atmosphere ... atmosphere originates from refuse incineration as in the case of Pb Dust from Other Sources Lead Concentration Lead Concentration Trace Metal Concentrations Trace Metal Concentrations Emission Sources...

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Báo cáo khoa học: A new and efﬁcient method for inhibition of RNA viruses by DNA interference pptx

Ngày tải lên : 16/03/2014, 02:20

... simply because DNA resembles a modiﬁed siRNA molecule deprived of the 2¢-OH group of ribose We showed the silencing effect of targeted RNA in HeLa cells to be at the level of approximately 78% when ... observable in the uppermost systemic leaves of N benthamiana The weaker response of infected tobacco plants to dsDNA treatment in these experiments could be explained by the length of the cDNA ... 100 and 250 nm, respectively Cells with green ﬂuorescence expressed the gp41–EGFP fusion protein After treating the cells with increasing concentrations of siDNA, we observed a decrease in the expression...

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SYSTEMATIC METHOD FOR ANALYSIS OF PERFORMANCE LOSS WHEN USING SIMPLIFIED MPC FORMULATIONS pptx

Ngày tải lên : 31/03/2014, 22:20

... IB type = [1 2 3] and DOF = 4………….…… 69 Figure 28: IB types for same degree of freedom – free inputs at the beginning… 70 Figure 29: IB types for same degree of freedom – free inputs in the end…… ... the controller since only the difference between successive inputs and not the actual inputs are blocked As the previously presented IB scheme, the DIB has one drawback too Both of these strategies ... there is always some constant difference between two consecutive inputs It means that, in this case, the differences between consecutive inputs are free dibtype = [number1, number2, ] Example 1:...

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APPLYING OF ULTRASOUND FOR PRODUCTION OF NANOMATERIALS

Ngày tải lên : 11/06/2014, 12:29

... liquid Effective means of deagglomerating and dispersing are needed to overcome the bonding forces after wettening the powder The ultrasonic breakup of the agglomerate structures in aqueous and ... discrete - single-dispersed – carbonnanotubes in few minutes LLC «Ultrasonic technique - INLAB» develops and produces ultrasonic equipment for realizing of all above-mentioned technologies, both ... bonds or chemical interactions with the substrate and cannot be removed by washing • The formation of proteinaceous micro- and nanospheres It have been demonstrated that any protein (e. g., polyglutamic...

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